scholarly journals Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Hyun-Woo Joo ◽  
Yi-Sun Song ◽  
In-Hwa Park ◽  
Guang-Yin Shen ◽  
Jin-Hee Seong ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Hepatic Steatosis ◽  
Hepg2 Cells ◽  
Diabetic Rats ◽  
Standard Diet ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Protein Levels ◽  
Associated Proteins ◽  
Stimulating Factor

Background. We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of β-oxidation-related gene expression. However, the mechanisms underlying this process remain unclear. This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes. Methods. Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks. All HFD-fed rats were then injected with streptozotocin (STZ). One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 μg/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days. Four weeks later, serum biochemical and histology analyses were conducted. The expression of autophagy-associated proteins was determined by Western blotting. The mRNA expression of β-oxidation-related genes was determined by quantitative real-time polymerase chain reaction. HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids. Results. Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats. Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats. The mRNA expression of β-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats. Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences. Conclusions. Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats.

scholarly journals Adhesion of NFS-60 myeloid leukemia cells to MC3T3-G2/PA6 stromal cells induces granulocyte colony-stimulating factor production

Blood ◽  
1994 ◽  
Vol 84 (2) ◽  
pp. 415-420 ◽  
Author(s):  
T Yoshikubo ◽  
K Ozawa ◽  
K Takahashi ◽  
M Nishikawa ◽  
N Horiuchi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Stromal Cells ◽  
Myeloid Leukemia ◽  
Myeloid Cell ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor ◽  
Csf Production

Abstract To examine the interaction between immature myeloid cells and stromal cells in the induction of granulocyte colony-stimulating factor (G-CSF) production, stromal cells of the MC3T3-G2/PA6 (PA6) murine cell line, which has preadipocyte characteristics and can support hematopoiesis, were cocultured with various myeloid cell lines and G-CSF mRNA expression was examined by Northern and reverse transcriptase- polymerase chain reaction analyses. A significant amount of G-CSF mRNA was induced by the culture of an interleukin-3/G-CSF-dependent murine myeloid leukemia cell line, NFS-60, on PA6 stromal cells for 16 hours. Using a G-CSF-dependent subline of DA-1 (DA-1N), the biologic activity of G-CSF was also detected in PA6/NFS-60 coculture supernatants, but not in the culture supernatant of PA6 or NFS-60 alone. Direct contact of NFS-60 cells with the PA6 stromal layer was essential for the induction of G-CSF mRNA, as indicated by the following observations: (1) NFS-60 cells efficiently adhered to PA6 cells; (2) medium conditioned by NFS-60 cells did not contain the activity to induce G- CSF mRNA in PA6 cells; and (3) induction of G-CSF mRNA was not observed when NFS-60 cells were separated from PA6 cells by a microporous membrane (0.45-microns pore size). Several other myeloid cell lines, including FDC-P2, 32Dcl3, WEHI-3, and DA-1, did not induce G-CSF mRNA expression after the coculture with PA6 cells, although significant numbers of these cells adhered to PA6 cells. Therefore, NFS-60 cells may express or overexpress a molecule that is involved in adhesion- mediated induction of G-CSF production.

scholarly journals Characterization of Isoforms of the Ovine Granulocyte Colony Stimulating Factor

2019 ◽  
Author(s):  
Runting Li ◽  
Longxin Chen ◽  
Yuqin Wang ◽  
Limeng Zhang ◽  
Ting Liu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Mammalian Cells ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Proliferation And Differentiation ◽  
Candidate Target ◽  
Stimulating Factor

ABSTRACTThe granulocyte colony-stimulating factor (GCSF) regulates the maturation, proliferation, and differentiation of precursor cells of neutrophilic granulocytes, and has been widely studied in several species. To investigate the function of variants of sheep GCSF (sGCSF), this study compared difference in their mRNA expression levels. Both the activity and mRNA expression level of GCSFv2 were higher than those of GCSFv1. Their sequences were aligned, which showed that they had the highest homology with bovine GCSF. Then, predicted ovine GCSF isoforms and their constant C-terminals were cloned and expressed, which were stably expressed in mammalian cells. After purification, all GCSF functions were different both in vitro and in vivo, and the GCSF C-terminal was best. These results indicated that the ability to stimulate both the proliferation and differentiation of progenitor cells and to activate the maturation of neutrophils could be used for research of efficacious non-antibiotic protein drugs. Furthermore, GCSF can be used as candidate target of genetic breeding to specifically improve sheep immunity.

scholarly journals Therapeutic effects of granulocyte-colony stimulating factor on non-alcoholic hepatic steatosis in the rat

2013 ◽  
Vol 12 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Yi-Sun Song ◽  
Cheng-Hu Fang ◽  
Byung-Im So ◽  
Jun-Young Park ◽  
Dae Won Jun ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Therapeutic Effects ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

scholarly journals Granulocyte-colony stimulating factor prevents the development of hepatic steatosis in rats

2015 ◽  
Vol 14 (2) ◽  
pp. 243-250 ◽  
Author(s):  
Yi-Sun Song ◽  
Hyun-Woo Joo ◽  
In-Hwa Park ◽  
Guang-Yin Shen ◽  
Yonggu Lee ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

scholarly journals Adhesion of NFS-60 myeloid leukemia cells to MC3T3-G2/PA6 stromal cells induces granulocyte colony-stimulating factor production

Blood ◽  
1994 ◽  
Vol 84 (2) ◽  
pp. 415-420 ◽  
Author(s):  
T Yoshikubo ◽  
K Ozawa ◽  
K Takahashi ◽  
M Nishikawa ◽  
N Horiuchi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Stromal Cells ◽  
Myeloid Leukemia ◽  
Myeloid Cell ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor ◽  
Csf Production

To examine the interaction between immature myeloid cells and stromal cells in the induction of granulocyte colony-stimulating factor (G-CSF) production, stromal cells of the MC3T3-G2/PA6 (PA6) murine cell line, which has preadipocyte characteristics and can support hematopoiesis, were cocultured with various myeloid cell lines and G-CSF mRNA expression was examined by Northern and reverse transcriptase- polymerase chain reaction analyses. A significant amount of G-CSF mRNA was induced by the culture of an interleukin-3/G-CSF-dependent murine myeloid leukemia cell line, NFS-60, on PA6 stromal cells for 16 hours. Using a G-CSF-dependent subline of DA-1 (DA-1N), the biologic activity of G-CSF was also detected in PA6/NFS-60 coculture supernatants, but not in the culture supernatant of PA6 or NFS-60 alone. Direct contact of NFS-60 cells with the PA6 stromal layer was essential for the induction of G-CSF mRNA, as indicated by the following observations: (1) NFS-60 cells efficiently adhered to PA6 cells; (2) medium conditioned by NFS-60 cells did not contain the activity to induce G- CSF mRNA in PA6 cells; and (3) induction of G-CSF mRNA was not observed when NFS-60 cells were separated from PA6 cells by a microporous membrane (0.45-microns pore size). Several other myeloid cell lines, including FDC-P2, 32Dcl3, WEHI-3, and DA-1, did not induce G-CSF mRNA expression after the coculture with PA6 cells, although significant numbers of these cells adhered to PA6 cells. Therefore, NFS-60 cells may express or overexpress a molecule that is involved in adhesion- mediated induction of G-CSF production.

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