scholarly journals Comparative Evaluation of Efficacy of Chlorhexidine and Herbal Mouthwash as a Preprocedural Rinse in Reducing Dental Aerosols: A Microbiological Study

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Sangeeta Umesh Nayak ◽  
Anushka Kumari ◽  
Valliammai Rajendran ◽  
Vijendra Pal Singh ◽  
Ashwini Hegde ◽  
...  
Keyword(s):  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Study Patient ◽  
Microbial Culture ◽  
Mouth Rinse ◽  
Mouth Rinses ◽  
Intergroup Comparison ◽  
Viable Bacteria ◽  
Post Hoc

Objective. The risk to dentists, assistants, and patients of infectious diseases through aerosols has long been recognized. The aim of this study was to evaluate and compare the efficacy of commercially available preprocedural mouth rinses containing 0.2% chlorhexidine (CHX) gluconate, Befresh™ herbal mouthwash, and water in reducing the levels of viable bacteria in aerosols. Materials & Methods. This was a single-center, double-masked, placebo-controlled, randomized, three-group parallel design study. 30 patients (10 patients in each group) were recruited in the study. Patient rinsed mouth with 10 ml of CHX, 10 ml Befresh™, or 10 ml water. All the patients underwent supragingival ultrasonic scaling for a minimum of 30 min. The aerosol collection was done using a blood agar plate. The blood agar plates were kept approximately 12 inches from the patient’s mouth. The microbial culture was analyzed. The colony-forming unit (CFU) counting in all three groups was assessed using one-way ANOVA test to compare among the groups (p value <0.001). The intergroup comparison was done using the post hoc Tukey test. Result. There was a marked reduction in the CFU in the CHX group in all three areas. This was followed by Befresh™ (Sagar Pharmaceuticals) mouthwash. There was no reduction in the CFU of the water group. Conclusion. This study proves that a regular preprocedural mouth rinse could significantly reduce the majority bacteria present in aerosols generated by the use of an ultrasonic unit, and Befresh™ mouth rinse was found to be equally effective in reducing the aerosol contamination to 0.2% CHX gluconate.

scholarly journals Diagnostic Performance of Various Methodologies for Group B Streptococcus Screening in Pregnant Woman in China

2021 ◽  
Vol 11 ◽  
Author(s):  
Kankan Gao ◽  
Qiulian Deng ◽  
Lianfen Huang ◽  
Chien-Yi Chang ◽  
Huamin Zhong ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Agar Plate ◽  
Reference Method ◽  
Antigen Detection ◽  
The Other ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Chromogenic Agar ◽  
Group B ◽  
Detection Reagent

Maternal vaginal/rectal colonization of group B streptococcus (GBS) is a main risk for neonatal invasive infection. Efficient determination of GBS colonization in pregnant women is crucial. This study aimed to investigate the prevalence of GBS carriage and evaluate the diagnostic performance of six methodologies for GBS screening conducted in China, including blood agar plate, liquid chromogenic medium, and loop-mediated isothermal amplification (LAMP) without pre-enrichment, chromogenic agar plate with pre-enrichment, and GBS antigen detection without and with pre-enrichment in comparison with the standard reference method (Lim broth-enriched subculture with plating on 5% sheep blood agar). Vaginal/rectal swabs were collected from 1,281 pregnant women at 35–37 weeks of gestation. Of them, 309 were taken in triplicate, one for Lim broth-enriched subculture, one for blood agar plate, and the third for GBS antigen detection (Reagent W); 177 were acquired in duplicate, one for Lim broth-enriched subculture and the other for GBS antigen detection (Reagent H); 502 were obtained in duplicate, one for Lim broth-enriched subculture and the other for liquid chromogenic medium; 158 were collected in duplicate, one for Lim broth-enriched subculture and the other for LAMP; and 135 were inoculated in Lim broth-enriched for GBS antigen detection (Reagent W) and subculture with chromogenic agar plate and 5% blood agar plate. The overall prevalence of GBS carriage was 10.1% (130/1,281, 95% CI: 8.5–12.1%) according to the standard reference method. Compared with the standard reference method, the LAMP had excellent performance of sensitivity (100%, 95%CI: 83.4–100%), specificity (94%, 95%CI: 88.1–97.1%), and Yoden index (0.940); as well as the blood agar plate with sensitivity (81.5%, 95%CI: 61.3–93.0%), specificity (100%, 95%CI: 98.3–100.0%), and Yoden index (0.815). The other four methods were not sufficient to reach the threshold in terms of sensitivity or specificity compared to the standard reference method. Furthermore, for LAMP, results can be obtained within 0.5–1 h, while for blood agar plate, which needed 24–48 h, and further identification was required. Our data suggested that the performance of LAMP was highly comparable to the standard Lim broth-enriched subculture and LAMP is considered as an alternative for fast and accurate GBS screening.

scholarly journals Sterility Duration of Preprimed Extracorporeal Membrane Oxygenation Circuits

2018 ◽  
Vol 23 (4) ◽  
pp. 311-314 ◽  
Author(s):  
Vi Ean Tan ◽  
Alan T. Evangelista ◽  
Dominick M. Carella ◽  
Daniel Marino ◽  
Wayne S. Moore ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Room Temperature ◽  
Agar Plate ◽  
Fungal Growth ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Macconkey Agar ◽  
Pilot Data ◽  
Sheep Blood ◽  
Membrane Oxygenation

OBJECTIVES There is a lack of standardization and supporting data regarding the duration preassembled and preprimed extracorporeal membrane oxygenation (ECMO) circuits are expected to be sterile. Therefore, the purpose of this study was to prospectively evaluate whether preassembled and preprimed ECMO circuits could maintain sterility for a period up to 65 days. DESIGN Four ECMO circuits (2 neonatal/pediatric¼” and 2 adolescent/adult ⅜ ”) were assembled and primed under sterile conditions and maintained at room temperature. Culture samples were obtained from each circuit and plated within 1 hour. Culture samples were obtained on day 0 when assembled and primed then every 5 days up to day 65. Samples were plated on several different media including the following: blood agar plate: trypticase soy agar with 5% sheep blood, MacConkey agar, and thioglycollate broth then incubated at 35°C for 3 days. RESULTS All cultures obtained from the priming solution from of the¼” and ⅜ ” ECMO circuits produced no microbial or fungal growth for the 65-day study period. CONCLUSION These pilot data suggest preprimed ECMO circuits may maintain sterility for a period up to 65 days. Additional studies evaluating a larger number of ECMO circuits are needed to confirm these findings.

scholarly journals Improved reliability of the primary plate bacitracin test on throat cultures with sulfamethoxazole-trimethoprim blood agar plates

1979 ◽  
Vol 9 (1) ◽  
pp. 144-146 ◽  
Author(s):  
T Kurzynski ◽  
C Meise ◽  
R Daggs ◽  
A Helstad
Keyword(s):  
Agar Plate ◽  
Susceptibility Test ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Group A Streptococci ◽  
Sheep Blood Agar Plate ◽  
Group A ◽  
Primary Plate ◽  
Disk Susceptibility

The primary plate bacitracin differentiation disk susceptibility test identified 85% of group A streptococci from throat cultures on SXT-BA(CO2) plates within 24 h, as compared to only 26% on a conventional sheep blood agar plate.

scholarly journals Self Prepared Herbal Mouthwash Used As Pre-procedural Rinse in Reducing Dental Aerosol: A Substitute to Chemical Mouthrinse: A Clinico-Microbiological Study

2021 ◽  
Vol 12 (3) ◽  
pp. 593-598
Author(s):  
Chinnu Mary Varghese ◽  
Vidushi Sheokand ◽  
Amit Bhardwaj ◽  
Harender Sehrawat ◽  
Ridhima Uppal ◽  
...  
Keyword(s):  
Blood Agar ◽  
Mouth Rinse ◽  
Microbiological Study ◽  
Mouth Rinses ◽  
Group A

Aim:To evaluate  and compare the efficacy of pre-procedural mouth rinses in reducing microbial content of aerosol product during ultrasonic-scaling procedures by viable bacterial count.Materials And Methods:5 patients were assigned in each group: A- Neem, B -CHX, C-Triphala,  D - Control Group.In Group A, B, C -Patient were asked to rinse their mouth with 10 ml mouthwash for 30 seconds  before SRP, of which A and C are self- prepared herbal mouthwashes i.e Triphala and Neem. Aerosol will be collected, cultured and incubated on blood agar plates at specified sites from operator. CFU will be counted and result will be assessed statistically. Conclusion: The study suggests that 10 ml of Neem Mouth rinse when used 10 minutes prior to ultrasonic scaling is more potent in reducing the aerosol contamination as compared to the Triphala mouth rinse and commercially available 0.2 % Chlorhexidine mouthrinse. Also the reduction in aerosol content was seen in Tray location  when rinsed with CHX and aerosol reduction  in Spitoon and Chest location  while rinsing with Neem mouthrinse.

scholarly journals THE BLOOD-AGAR PLATE FOR SPORE-FORMING ANAËROBES

1926 ◽  
Vol 11 (5) ◽  
pp. 305-321
Author(s):  
Luther Thompson
Keyword(s):  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate
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