scholarly journals 3D Nanostructures for Tissue Engineering, Cancer Therapy, and Gene Delivery

2020 ◽  
Vol 2020 ◽  
pp. 1-24 ◽  
Author(s):  
Ahmad Gholami ◽  
Seyyed Alireza Hashemi ◽  
Khadije Yousefi ◽  
Seyyed Mojtaba Mousavi ◽  
Wei-Hung Chiang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Organic Semiconductors ◽  
Self Assembly ◽  
Critical Role ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Self Assembling

The self-assembling is a spontaneous progression through which objects of nanophase/molecules materialize into prepared collections. Several biomolecules can interact and assemble into highly structured supramolecular structures, for instance, proteins and peptides, with fibrous scaffolds, helical ribbons, and many other functionalities. Various self-assembly systems have been established, from copolymers in blocks to three-dimensional (3D) cell culture scaffolds. Another advantage of self-assembly is its ability to manage a large variety of materials, including metals, oxides, inorganic salts, polymers, semiconductors, and various organic semiconductors. The most basic self-assembly of 3D nanomaterials is three primary forms of nanostructured carbon-based materials that perform a critical role in the progress of modern nanotechnologies, such as carbon nanotubes (CNTs), graphene, and fullerene. This review summarized important information on the 3D self-assembly nanostructure, such as peptide hydrogel, graphene, carbon nanotubes (CNTs), and fullerene for application in gene delivery, cancer therapy, and tissue engineering.

scholarly journals A Self-Assembling Amphiphilic Peptide Dendrimer-Based Drug Delivery System for Cancer Therapy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1092
Author(s):  
Dandan Zhu ◽  
Huanle Zhang ◽  
Yuanzheng Huang ◽  
Baoping Lian ◽  
Chi Ma ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Drug Resistance ◽  
Cancer Therapy ◽  
Self Assembly ◽  
Cancer Drug ◽  
Acquired Drug Resistance ◽  
Self Assembling ◽  
Amphiphilic Peptide ◽  
Peptide Dendrimer

Despite being a mainstay of clinical cancer treatment, chemotherapy is limited by its severe side effects and inherent or acquired drug resistance. Nanotechnology-based drug-delivery systems are widely expected to bring new hope for cancer therapy. These systems exploit the ability of nanomaterials to accumulate and deliver anticancer drugs at the tumor site via the enhanced permeability and retention effect. Here, we established a novel drug-delivery nanosystem based on amphiphilic peptide dendrimers (AmPDs) composed of a hydrophobic alkyl chain and a hydrophilic polylysine dendron with different generations (AmPD KK2 and AmPD KK2K4). These AmPDs assembled into nanoassemblies for efficient encapsulation of the anti-cancer drug doxorubicin (DOX). The AmPDs/DOX nanoformulations improved the intracellular uptake and accumulation of DOX in drug-resistant breast cancer cells and increased permeation in 3D multicellular tumor spheroids in comparison with free DOX. Thus, they exerted effective anticancer activity while circumventing drug resistance in 2D and 3D breast cancer models. Interestingly, AmPD KK2 bearing a smaller peptide dendron encapsulated DOX to form more stable nanoparticles than AmPD KK2K4 bearing a larger peptide dendron, resulting in better cellular uptake, penetration, and anti-proliferative activity. This may be because AmPD KK2 maintains a better balance between hydrophobicity and hydrophilicity to achieve optimal self-assembly, thereby facilitating more stable drug encapsulation and efficient drug release. Together, our study provides a promising perspective on the design of the safe and efficient cancer drug-delivery nanosystems based on the self-assembling amphiphilic peptide dendrimer.

Tandem Molecular Self-assembly for Selective Lung Cancer Therapy with Two Orders of Magnitude Increase in Efficiency

Nanoscale ◽  
2021 ◽  
Author(s):  
Debin Zheng ◽  
Jingfei Liu ◽  
Yinghao Ding ◽  
Limin Xie ◽  
Yingying Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Therapy ◽  
Anticancer Drugs ◽  
Self Assembly ◽  
Therapeutic Efficacy ◽  
Self Assembling ◽  
Lung Cancer Therapy ◽  
Magnitude Increase

In situ self-assembling of prodrug molecules into nanomedicine can elevate the therapeutic efficacy of anticancer medications by enhancing the targeting and enrichment of anticancer drugs at tumor sites. However, the...

scholarly journals Mitochondria-Targeted Self-Assembly of Peptide-Based Nanomaterials

2021 ◽  
Vol 9 ◽  
Author(s):  
Zhen Luo ◽  
Yujuan Gao ◽  
Zhongyu Duan ◽  
Yu Yi ◽  
Hao Wang
Keyword(s):  
Clinical Trials ◽  
Cancer Therapy ◽  
Self Assembly ◽  
Recent Progress ◽  
Assembly Systems ◽  
Targeted Cancer Therapy ◽  
Cancer Treatments ◽  
Self Assembling ◽  
Stimuli Response

Mitochondria are well known to serve as the powerhouse for cells and also the initiator for some vital signaling pathways. A variety of diseases are discovered to be associated with the abnormalities of mitochondria, including cancers. Thus, targeting mitochondria and their metabolisms are recognized to be promising for cancer therapy. In recent years, great efforts have been devoted to developing mitochondria-targeted pharmaceuticals, including small molecular drugs, peptides, proteins, and genes, with several molecular drugs and peptides enrolled in clinical trials. Along with the advances of nanotechnology, self-assembled peptide-nanomaterials that integrate the biomarker-targeting, stimuli-response, self-assembly, and therapeutic effect, have been attracted increasing interest in the fields of biotechnology and nanomedicine. Particularly, in situ mitochondria-targeted self-assembling peptides that can assemble on the surface or inside mitochondria have opened another dimension for the mitochondria-targeted cancer therapy. Here, we highlight the recent progress of mitochondria-targeted peptide-nanomaterials, especially those in situ self-assembly systems in mitochondria, and their applications in cancer treatments.

Photodegradable self-assembling PAMAM dendrons for gene delivery involving dendriplex formation and phototriggered circular DNA release

2016 ◽  
Vol 40 (3) ◽  
pp. 2601-2608 ◽  
Author(s):  
Yu-Sen Lai ◽  
Chai-Lin Kao ◽  
Ya-Pei Chen ◽  
Chia-Chia Fang ◽  
Chao-Chin Hu ◽  
...  
Keyword(s):  
Dna Binding ◽  
Gene Delivery ◽  
Self Assembly ◽  
Circular Dna ◽  
Self Assembling ◽  
Dna Release

Photoresponsive amphiphilic dendron bearing a photolabile o-nitrobenzyl group possesses self-assembly, DNA binding and photo-induced release.

Porous and strong three-dimensional carbon nanotube coated ceramic scaffolds for tissue engineering

2015 ◽  
Vol 3 (42) ◽  
pp. 8337-8347 ◽  
Author(s):  
P. Newman ◽  
Z. Lu ◽  
S. I. Roohani-Esfahani ◽  
T. L. Church ◽  
M. Biro ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Three Dimensional ◽  
Biological Properties ◽  
Porous Structures ◽  
High Quality

A method to coat high-quality uniform coatings of carbon nanotubes throughout 3D porous structures is developed. Testing of their physical and biological properties demonstrate their potential for application in tissue engineering.

Peptide α-helices for synthetic nanostructures

2007 ◽  
Vol 35 (3) ◽  
pp. 487-491 ◽  
Author(s):  
M.G. Ryadnov
Keyword(s):  
Self Assembly ◽  
De Novo ◽  
Three Dimensional ◽  
Functional Materials ◽  
Protein Assemblies ◽  
Helical Peptides ◽  
Design Rules ◽  
Natural Protein ◽  
Self Assembling ◽  
Recent Developments

Supramolecular structures arising from a broad range of chemical archetypes are of great technological promise. Defining such structures at the nanoscale is crucial to access principally new types of functional materials for applications in bionanotechnology. In this vein, biomolecular self-assembly has emerged as an efficient approach for building synthetic nanostructures from the bottom up. The approach predominantly employs the spontaneous folding of biopolymers to monodisperse three-dimensional shapes that assemble into hierarchically defined mesoscale composites. An immediate interest here is the extraction of reliable rules that link the chemistry of biopolymers to the mechanisms of their assembly. Once established these can be further harnessed in designing supramolecular objects de novo. Different biopolymer classes compile a rich repertoire of assembly motifs to facilitate the synthesis of otherwise inaccessible nanostructures. Among those are peptide α-helices, ubiquitous folding elements of natural protein assemblies. These are particularly appealing candidates for prescriptive supramolecular engineering, as their well-established and conservative design rules give unmatched predictability and rationale. Recent developments of self-assembling systems based on helical peptides, including fibrous systems, nanoscale linkers and reactors will be highlighted herein.

Using organic solvent absorption as a self-assembly method to synthesize three-dimensional (3D) reduced graphene oxide (RGO)/poly(3,4-ethylenedioxythiophene) (PEDOT) architecture and its electromagnetic absorption properties

RSC Advances ◽  
2014 ◽  
Vol 4 (91) ◽  
pp. 49780-49782 ◽  
Author(s):  
Fan Wu ◽  
Yuan Wang ◽  
Mingyang Wang
Keyword(s):  
Organic Solvent ◽  
Self Assembly ◽  
Three Dimensional ◽  
Absorption Properties ◽  
Self Assembling ◽  
Assembly Method ◽  
Electromagnetic Absorption ◽  
Solvent Absorption ◽  
Reduced Graphene ◽  
Promising Application

A novel self-assembling 3D-RGO/PEDOT architecture has been synthesized through organic solvent absorption and gentle heating. It gives a promising application in electromagnetic absorption.

scholarly journals A stimuli-responsive, pentapeptide, nanofiber hydrogel for tissue engineering

2019 ◽  
Author(s):  
James D. Tang ◽  
Cameron Mura ◽  
Kyle J. Lampe
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Amyloid Fibrils ◽  
Culture Media ◽  
Solvent Accessibility ◽  
Three Dimensional ◽  
Building Blocks ◽  
Weight Percent ◽  
Oligodendrocyte Progenitor Cells ◽  
Self Healing

ABSTRACTShort peptides are uniquely versatile building blocks for self-assembly. Supramolecular peptide assemblies can be used to construct functional hydrogel biomaterials—an attractive approach for neural tissue engineering. Here, we report a new class of short, five-residue peptides that form hydrogels with nanofiber structures. Using rheology and spectroscopy, we describe how sequence variations, pH, and peptide concentration alter the mechanical properties of our pentapeptide hydrogels. We find that this class of seven unmodified peptides forms robust hydrogels from 0.2–20 kPa at low weight percent (less than 3 wt. %) in cell culture media, and undergoes shear-thinning and rapid self-healing. The peptides self-assemble into long fibrils with sequence-dependent fibrillar morphologies. These fibrils exhibit a unique twisted ribbon shape, as visualized by TEM and Cryo-EM imaging, with diameters in the low tens of nanometers and periodicities similar to amyloid fibrils. Experimental gelation behavior corroborates our molecular dynamics simulations, which demonstrate peptide assembly behavior, an increase in β-sheet content, and patterns of variation in solvent accessibility. Our Rapidly Assembling Pentapeptides for Injectable Delivery (RAPID) hydrogels are syringe-injectable and support cytocompatible encapsulation of oligodendrocyte progenitor cells (OPCs), as well as their proliferation and three-dimensional process extension. Furthermore, RAPID gels protect OPCs from mechanical membrane disruption and acute loss of viability when ejected from a syringe needle, highlighting the protective capability of the hydrogel as potential cell carriers for trans-plantation therapies. The tunable mechanical and structural properties of these supramolecular assemblies are shown to be permissive to cell expansion and remodeling, making this hydrogel system suitable as an injectable material for cell delivery and tissue engineering applications.

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