scholarly journals Zingiber Officinale Roscoe Prevents Cellular Senescence of Myoblasts in Culture and Promotes Muscle Regeneration

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Nur Fatin Nabilah Mohd Sahardi ◽  
Faizul Jaafar ◽  
Mariam Firdhaus Mad Nordin ◽  
Suzana Makpol
Keyword(s):  
Cell Cycle ◽  
Cellular Senescence ◽  
Muscle Regeneration ◽  
Zingiber Officinale ◽  
Antioxidant Property ◽  
Cellular Morphology ◽  
Myoblast Differentiation ◽  
Regenerative Capacity ◽  
Maturation Index ◽  
Fusion Index

Background. Ageing resulted in a progressive loss of muscle mass and strength. Increased oxidative stress in ageing affects the capacity of the myoblast to differentiate leading to impairment of muscle regeneration. Zingiber officinale Roscoe (ginger) has potential benefits in reversing muscle ageing due to its antioxidant property. This study aimed to determine the effect of ginger in the prevention of cellular senescence and promotion of muscle regeneration. Methods. Myoblast cells were cultured into young and senescent state before treated with different concentrations of ginger standardised extracts containing different concentrations of 6-gingerol and 6-shogaol. Analysis on cellular morphology and myogenic purity was carried out besides determination of SA-β-galactosidase expression and cell cycle profile. Myoblast differentiation was quantitated by determining the fusion index, maturation index, and myotube size. Results. Treatment with ginger extracts resulted in improvement of cellular morphology of senescent myoblasts which resembled the morphology of young myoblasts. Our results also showed that ginger treatment caused a significant reduction in SA-β-galactosidase expression on senescent myoblasts indicating prevention of cellular senescence, while cell cycle analysis showed a significant increase in the percentage of cells in the G0/G1 phase and reduction in the S-phase cells. Increased myoblast regenerative capacity was observed as shown by the increased number of nuclei per myotube, fusion index, and maturation index. Conclusions. Ginger extracts exerted their potency in promoting muscle regeneration as indicated by prevention of cellular senescence and promotion of myoblast regenerative capacity.

scholarly journals Chlorella vulgaris Improves the Regenerative Capacity of Young and Senescent Myoblasts and Promotes Muscle Regeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
Nurhazirah Zainul Azlan ◽  
Yasmin Anum Mohd Yusof ◽  
Ekram Alias ◽  
Suzana Makpol
Keyword(s):  
Chlorella Vulgaris ◽  
Muscle Regeneration ◽  
Myogenic Differentiation ◽  
Myoblast Differentiation ◽  
Regenerative Capacity ◽  
Maturation Index ◽  
Myoblast Cells ◽  
And Function ◽  
Pharmaceutical Uses

Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. With increasing life expectancy, greater attention has been given to counteracting the effects of sarcopenia on the growing elderly population. Chlorella vulgaris, a microscopic, unicellular, green alga with the potential for various pharmaceutical uses, has been widely studied in this context. This study is aimed at determining the effects of C. vulgaris on promoting muscle regeneration by evaluating myoblast regenerative capacity in vitro. Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with C. vulgaris, followed by the induction of differentiation. The ability of C. vulgaris to promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA-β-gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the G0/G1 phase. Treatment with C. vulgaris resulted in decreased SA-β-gal expression and promotion of myogenic differentiation, as observed via an increased fusion index, maturation index, myotube size, and surface area and an increased percentage of cells that stained positive for myogenin. In conclusion, C. vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration.

scholarly journals The Consequences of Budding versus Binary Fission on Adaptation and Aging in Primitive Multicellularity

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 661
Author(s):  
Hanna Isaksson ◽  
Peter L. Conlin ◽  
Ben Kerr ◽  
William C. Ratcliff ◽  
Eric Libby
Keyword(s):  
Cell Cycle ◽  
Growth Rate ◽  
Cellular Senescence ◽  
Carrying Capacity ◽  
The Other ◽  
Binary Fission ◽  
Fundamental Aspect ◽  
Accumulated Damage ◽  
Cell Age ◽  
Multicellular Organisms

Early multicellular organisms must gain adaptations to outcompete their unicellular ancestors, as well as other multicellular lineages. The tempo and mode of multicellular adaptation is influenced by many factors including the traits of individual cells. We consider how a fundamental aspect of cells, whether they reproduce via binary fission or budding, can affect the rate of adaptation in primitive multicellularity. We use mathematical models to study the spread of beneficial, growth rate mutations in unicellular populations and populations of multicellular filaments reproducing via binary fission or budding. Comparing populations once they reach carrying capacity, we find that the spread of mutations in multicellular budding populations is qualitatively distinct from the other populations and in general slower. Since budding and binary fission distribute age-accumulated damage differently, we consider the effects of cellular senescence. When growth rate decreases with cell age, we find that beneficial mutations can spread significantly faster in a multicellular budding population than its corresponding unicellular population or a population reproducing via binary fission. Our results demonstrate that basic aspects of the cell cycle can give rise to different rates of adaptation in multicellular organisms.

scholarly journals Bach1 promotes muscle regeneration through repressing Smad-mediated inhibition of myoblast differentiation

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0236781
Author(s):  
Katsushi Suzuki ◽  
Mitsuyo Matsumoto ◽  
Yasutake Katoh ◽  
Liang Liu ◽  
Kyoko Ochiai ◽  
...  
Keyword(s):  
Muscle Regeneration ◽  
Myoblast Differentiation

scholarly journals Palmdelphin promotes myoblast differentiation and muscle regeneration

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yaping Nie ◽  
Hu Chen ◽  
Cilin Guo ◽  
Zhuning Yuan ◽  
Xingyu Zhou ◽  
...  
Keyword(s):  
Muscle Regeneration ◽  
Myoblast Differentiation

scholarly journals Cellular Senescence in Liver Disease and Regeneration

2021 ◽  
Author(s):  
Sofia Ferreira-Gonzalez ◽  
Daniel Rodrigo-Torres ◽  
Victoria L. Gadd ◽  
Stuart J. Forbes
Keyword(s):  
Cell Cycle ◽  
Liver Disease ◽  
Cell Cycle Arrest ◽  
Genomic Instability ◽  
Cellular Senescence ◽  
Cell Populations ◽  
Cycle Arrest ◽  
Relevant Role ◽  
Extent Of Damage

AbstractCellular senescence is an irreversible cell cycle arrest implemented by the cell as a result of stressful insults. Characterized by phenotypic alterations, including secretome changes and genomic instability, senescence is capable of exerting both detrimental and beneficial processes. Accumulating evidence has shown that cellular senescence plays a relevant role in the occurrence and development of liver disease, as a mechanism to contain damage and promote regeneration, but also characterizing the onset and correlating with the extent of damage. The evidence of senescent mechanisms acting on the cell populations of the liver will be described including the role of markers to detect cellular senescence. Overall, this review intends to summarize the role of senescence in liver homeostasis, injury, disease, and regeneration.

Pax3 and Pax7 expression during myoblast differentiation in vitro and fast and slow muscle regeneration in vivo

2009 ◽  
Vol 33 (4) ◽  
pp. 483-492 ◽  
Author(s):  
Edyta Brzóska ◽  
Marta Przewoźniak ◽  
Iwona Grabowska ◽  
Katarzyna Jańczyk-Ilach ◽  
Jerzy Moraczewski
Keyword(s):  
Muscle Regeneration ◽  
Slow Muscle ◽  
Myoblast Differentiation ◽  
Fast And Slow Muscle

scholarly journals Kinin-B2 Receptor Activity in Skeletal Muscle Regeneration and Myoblast Differentiation

2018 ◽  
Vol 15 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Janaina M. Alves ◽  
Antonio H. Martins ◽  
Claudiana Lameu ◽  
Talita Glaser ◽  
Nawal M. Boukli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Receptor Activity ◽  
Skeletal Muscle Regeneration ◽  
Myoblast Differentiation
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