scholarly journals In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.)

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Laila Muñoz Castellanos ◽  
Nubia Amaya Olivas ◽  
Juan Ayala-Soto ◽  
Carmen Miriam De La O Contreras ◽  
Miriam Zermeño Ortega ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Essential Oils ◽  
Fusarium Oxysporum ◽  
Tomato Fruit ◽  
Ethyl Esters ◽  
Piper Nigrum ◽  
Fruit Samples

In this study, hydrodistillation was used to obtain essential oils (EOs) from pepper (Piper nigrum L.) and clove (Eugenia caryophyllata) and co-hydrodistillation (addition of fatty acid ethyl esters as extraction co-solvents) was used to obtain functional extracts (FEs). Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger. The results showed that pepper (Piper nigrum) and clove (Eugenia caryophyllata) essential oils and their functional extracts are effective in vitro at concentrations from 400 to 500 ppm after 10 days of culturing. The essential oils and functional extracts were used on tomato fruit samples at three different concentrations: 350, 400, and 450 ppm5. Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%. The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C10), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi. Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β-caryophyllene in pepper oil.

In vivo and in vitro control activity of plant essential oils against three strains of Aspergillus niger

2017 ◽  
Vol 24 (27) ◽  
pp. 21948-21959 ◽  
Author(s):  
Peeyush Kumar ◽  
Sapna Mishra ◽  
Atul Kumar ◽  
Sanjeev Kumar ◽  
Chandra Shekhar Prasad
Keyword(s):  
Aspergillus Niger ◽  
Essential Oils ◽  
Control Activity ◽  
Plant Essential Oils

scholarly journals Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust

2020 ◽  
pp. 1379-1384
Author(s):  
Alex Rodrigues Silva Caetano ◽  
Sara Maria Chalfoun ◽  
Mario Lúcio Vilela Resende ◽  
Caroline Lima Angélico ◽  
Wilder Douglas Santiago ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Chemical Characterization ◽  
Eucalyptus Species ◽  
Hemileia Vastatrix ◽  
Antifungal Activities

Essential oils, also known as volatile oils, are substances produced through the secondary metabolism of plants. In this study, we determined the chemical composition and the in vitro and in vivo antifungal activity of the essential oils from four species of Eucalyptus, Eucalyptus citriodora, Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus microcorys, against the Hemileia vastatrix fungus. The essential oils from these four species of Eucalyptus were extracted from their leaves by the hydrodistillation technique using a modified Clevenger apparatus. The chemical characterization was performed by gas chromatography coupled with a mass spectrometer detector and by gas chromatography using a flame ionization detector. The antifungal activities of the essential oils against H. vastatrix were studied by evaluating the percentage of spore germination using the microdilution test for in vitro assays. The curative and preventive effects were evaluated in in vivo tests. The principal constituents of the essential oil from E. citriodora were citronellal, citronellol and isopulegol, while E. camaldulensis produced 1,8-cineole, α-terpineol and α-pinene. 1,8-cineole, α-pinene and α-terpineol were obtained from E. grandis and 1,8-cineole, α-pinene and trans-pinocarveol were the principal components in the essential oil of E. microcorys. In vitro and in vivo antifungal activities against the fungus under study were observed for most of the essential oils, except the essential oil from E. microcorys, for which no preventive antifungal activity was observed. Only the curing of infection by the H. vastatrix fungus was observed with this oil.

scholarly journals Effet de l’association de certains fongicides avec le chlorure de calcium sur le développement d’un complexe fongique responsable de la pourriture des pommes en conservation

2007 ◽  
Vol 88 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Khaled Attrassi ◽  
Rachid Benkirane ◽  
Benaissa Attrassi ◽  
Allal Douira
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Alternaria Alternata ◽  
Penicillium Expansum ◽  
Rhizopus Stolonifer ◽  
Cladosporium Herbarum ◽  
Trichothecium Roseum

L’utilisation du bénomyl et du thiabendazole (famille chimique des benzimidazoles), de l’azoxystrobine et du pyriméthanil montre, autant in vitro qu’in vivo, que ces fongicides sont faiblement à moyennement efficaces contre les agents pathogènes suivants : Rhizopus stolonifer, Penicillium expansum, Aspergillus niger, A. fumigatus, Alternaria alternata, Cladosporium herbarum, Fusarium oxysporum, Saccharomyces cerevisiae, Monilia fructigina, Cryptosporiopsis malicorticis, Spilocaea pomi et Trichothecium roseum. Utilisé in vitro, le chlorure de calcium seul à plus de 4 % est toléré par les douze espèces fongiques. In vivo et à basse température, le chlorure de calcium est efficace lorsqu’utilisé à 4 et 6 %. L’association de ces fongicides avec le chlorure de calcium (4 %) permet de diminuer l’intensité des lésions causées par la pourriture des pommes en conservation. Le chlorure de calcium apparaît intervenir en renforçant les liens avec les principaux sites d’action des fongicides de l’agent pathogène évalué.

Antifungal Activity of Essential Oil and Hydrosol Extract of Ballota nigra L. and their Protective Effects Against the Black Rot of Tomatoes

2019 ◽  
Vol 15 (7) ◽  
pp. 662-671 ◽  
Author(s):  
Nabila A. Sebaa ◽  
Amina T. Zatla ◽  
Mohammed E.A. Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Alternaria Alternata ◽  
Penicillium Expansum ◽  
Protective Effects ◽  
Antifungal Activities

Background: Bellota species are used to treat various diseases in traditional folk medicine. Objectives: This study aimed to chemically characterize the essential oils and the hydrosol extract and regional specificity of the major components of Ballota nigra essential oil and to evaluate their in vitro and in vivo antifungal activities. Methods: Essential oils were obtained by a Clevenger-type apparatus and analyzed by using Gas Chromatography (GC) and Gas Chromatography Mass Spectroscopy (GC/MS). The antifungal activities were tested to three phytopathogenic stains (Penicillium expansum, Aspergillus niger and Alternaria alternata). Results: Altogether, 38 compounds were identified in the essential oils, representing 92.1-96.8% of the total oil composition. Their main constituents were E-β-caryophyllene (4.8-24.6%), E-β-farnesene (3.3-22.9%), β-bisabolene (7.6-30.2%), α-humulene (2.1-13.3%) and geranyl linalool (1.1-8.2%). The statistical methods deployed confirmed that there is a relation between the essential oil compositions and the harvest locations. Hydrosol extract was constituted by seven components, represented principally by methyl eugenol (75.2%) and caryophyllene oxide (12.5%). The results of in vitro antifungal activity with essential oil and hydrosol extract have shown very interesting antifungal activities on Penicillium expansum and Alternaria alternata strains with percentage reductions up to 80%. Additionally, in in vivo assays, Ballota nigra essential oil and hydrosol extract significantly reduce decay in artificially inoculated tomato by Alternaria alternata. Conclusion: The essential oil and hydrosol extract can be used as a potential source of sustainable eco-friendly botanical fungicides to protect stored tomatoes from pathogens, saprophytic fungi causing bio-deterioration to a variety of food commodities.

In vitro and in vivo antifungal activity of some essential oils against feline isolates of Microsporum canis

2012 ◽  
Vol 22 (2) ◽  
pp. 179-184 ◽  
Author(s):  
L. Mugnaini ◽  
S. Nardoni ◽  
L. Pinto ◽  
L. Pistelli ◽  
M. Leonardi ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Microsporum Canis ◽  
In Vivo Antifungal Activity

scholarly journals Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 286 ◽  
Author(s):  
Van Anh Ngo ◽  
San-Lang Wang ◽  
Van Bon Nguyen ◽  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Endophytic Bacteria ◽  
Black Pepper ◽  
Antagonistic Activity ◽  
Piper Nigrum ◽  
Control Group ◽  
Vitro Assay ◽  
16S Rna

In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45–11.21%) and lower fatal rate (11.11–15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

Ammoides verticillata Essential Oil as Biocontrol Agent of Selected Fungi and Pest of Olive Tree

2020 ◽  
Vol 11 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Hanane Senouci ◽  
Nassira G. Benyelles ◽  
Mohammed EA Dib ◽  
Jean Costa ◽  
Alain Muselli
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Essential Oil ◽  
Biological Activities ◽  
Bactrocera Oleae ◽  
Penicillium Crustosum ◽  
Ammoides Verticillata ◽  
In Vitro Antifungal Activity

Background: Ammoides verticillata Briq is an aromatic and medicinal herb that has been widely used in folk medicine for treatment of several types of pathologies such as respiratory problems, colds, fever, headache, gastric disorders and renal infections. Objective: Essential oils have been subjected of numerous works for their antimicrobial and insecticidal properties which have been valued by hundreds of publications and patents. The main objective of this work was to evaluate the chemical composition, and the in vitro and in vivo antifungal, and insecticidal properties of essential oil of Ammoides verticillata. Methods: In this work, the air-dried aerial parts of Ammoides verticillata were hydrodistilled in a Clevenger-type apparatus. The essential oil isolated was analyzed using gas chromatography (GC) and mass spectrometry (GC/MS). The in vitro antifungal activity of the essential oil was investigated against four plant fungi using radial growth technique. The effect of the essential oil on disease development of olive caused by Penicillium crustosum and Aspergillus niger in the in vivo conditions. The fumigation test of the essential oil was evaluated against L3 stages, pupas and adults of Bactrocera oleae known as the olive fly. Results: The essential oil of A. verticillata was characterized mainly by carvacrol (44.3%), limonene (19.3%) and p-cymene (19.2%). The result of in vitro antifungal activity of essential oil showed an interesting antifungal inhibition against Alternaria alternata and Fusarium solani strains with percentage inhibition of 89%. Furthermore, oil of A. verticillata has demonstrated promising in vivo antifungal activity to control infections of olives caused by Aspergillus niger and Penicillium crustosum. In addition, the essential oil exhibited fumigation toxicity against Bactrocera oleae with mortality percentage of 100% at 2 μL/L air. Conclusion: The results showed that essential oil of A. verticillata has interesting biological activities, which suggests that oil have the potential to be used as biocide to protect olives of pathogenic fungi and pests.

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