scholarly journals Bulleyaconitine A Inhibits Visceral Nociception and Spinal Synaptic Plasticity through Stimulation of Microglial Release of Dynorphin A

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Sheng-Nan Huang ◽  
Jinbao Wei ◽  
Lan-Ting Huang ◽  
Pei-Jun Ju ◽  
Jinghong Chen ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Receptor Antagonist ◽  
Opioid Receptor ◽  
Rat Model ◽  
Visceral Pain ◽  
Visceral Hypersensitivity ◽  
Trinitrobenzene Sulfonic Acid ◽  
Dependent Manner ◽  
Dynorphin A ◽  
Opioid Receptor Antagonist

Background. Visceral pain is one of the most common types of pain and particularly in the abdomen is associated with gastrointestinal diseases. Bulleyaconitine A (BAA), isolated from Aconitum bulleyanum, is prescribed in China to treat chronic pain. The present study is aimed at evaluating the mechanisms underlying BAA visceral antinociception. Methods. The rat model of chronic visceral hypersensitivity was set up by colonic perfusion of 2,4,6-trinitrobenzene sulfonic acid (TNBS) on postnatal day 10 with coapplication of heterotypic intermittent chronic stress (HeICS). Results. The rat model of chronic visceral hypersensitivity exhibited remarkable abdominal withdrawal responses and mechanical hyperalgesia in hind paws, which were dose-dependently attenuated by single subcutaneous of administration of BAA (30 and 90 μg/kg). Pretreatment with the microglial inhibitor minocycline, dynorphin A antiserum, and κ-opioid receptor antagonist totally blocked BAA-induced visceral antinociception and mechanical antihyperalgesia. Spontaneous excitatory postsynaptic currents (sEPSCs) in spinal dorsal horn lamina II neurons were recorded by using whole-cell patch clamp. Its frequency (but not amplitude) from TNBS-treated rats was remarkably higher than that from naïve rats. BAA (1 μM) significantly reduced the frequency of sEPSCs from TNBS-treated rats but not naïve rats. BAA-inhibited spinal synaptic plasticity was blocked by minocycline, the dynorphin A antiserum, and κ-opioid receptor antagonist. Dynorphin A also inhibited spinal synaptic plasticity in a κ-opioid receptor-dependent manner. Conclusions. These results suggest that BAA produces visceral antinociception by stimulating spinal microglial release of dynorphin A, which activates presynaptic κ-opioid receptors in afferent neurons and inhibits spinal synaptic plasticity, highlighting a novel interaction mode between microglia and neurons.

The δ1-opioid receptor antagonist, 7-benzylspiroindanylnaltrexone, prolongs renal allograft survival in a rat model

1998 ◽  
Vol 354 (1) ◽  
pp. R3-R5 ◽  
Author(s):  
Kristin M Linner ◽  
Barbara J Stickney ◽  
Heidi E Quist ◽  
Burt M Sharp ◽  
Philip S Portoghese
Keyword(s):  
Receptor Antagonist ◽  
Opioid Receptor ◽  
Rat Model ◽  
Renal Allograft ◽  
Allograft Survival ◽  
Opioid Receptor Antagonist

scholarly journals Protease inhibitor-induced nausea and vomiting is attenuated by a peripherally acting, opioid-receptor antagonist in a rat model

2009 ◽  
Vol 6 (1) ◽  
pp. 19 ◽  
Author(s):  
Chun-Su Yuan ◽  
Chong-Zhi Wang ◽  
Sangeeta R Mehendale ◽  
Han H Aung ◽  
Adela Foo ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Receptor Antagonist ◽  
Opioid Receptor ◽  
Rat Model ◽  
Nausea And Vomiting ◽  
Opioid Receptor Antagonist

Opioid Receptors Contribute to Antinociceptive Effect of Tianeptine on Colorectal Distension-Induced Visceral Pain in Rats

Pharmacology ◽  
2017 ◽  
Vol 101 (1-2) ◽  
pp. 96-103 ◽  
Author(s):  
S. Sırrı Bilge ◽  
Fatih İlkaya ◽  
Özge Darakcı ◽  
Engin Çiftcioğlu ◽  
Ayhan Bozkurt
Keyword(s):  
Receptor Antagonist ◽  
Opioid Receptor ◽  
Opioid Receptors ◽  
Visceral Pain ◽  
Antinociceptive Effect ◽  
Colorectal Distension ◽  
Implanted Electrodes ◽  
Opioid Receptor Antagonist ◽  
Before And After ◽  
Peripheral Opioid Receptors

Tianeptine is a clinically effective atypical antidepressant with distinct neurochemical properties. In this study, we aimed to investigate the contribution of opioid receptors in the antinociceptive effect of tianeptine on visceral pain in awake rats and to differentiate the subtype and the localization (central and/or peripheral) of these opioid receptors involved in this antinociception. Visceromotor response to noxious colorectal distension (CRD) was quantified with electromyographic recordings, obtained from previously implanted electrodes into the external oblique musculature of rats under anesthesia, before and after tianeptine administration. The opioid receptor antagonist naloxone hydrochloride (NLX) and peripherally restricted opioid receptor antagonist naloxone methiodide (NLXM) were administered intravenously 10 min before tianeptine (10 mg/kg, i.v.). The antinociceptive effect of tianeptine was abolished by NLX (1 and 2 mg/kg, i.v.), but was partially reduced by NLXM (1 and 2 mg/kg, i.v.). A µ-opioid receptor-selective dose (0.03 mg/kg, i.v.) of NLX, but not NLXM, significantly inhibited the antinociceptive effect of tianeptine. Our results suggest that antinociceptive effect of tianeptine on CRD-induced visceral nociception in rats involves the activation of both central and peripheral opioid receptors.

Sign in / Sign up

Export Citation Format

Share Document