scholarly journals The Pungent and Hot Chinese Herbs Cause Heat Syndrome in Rats by Affecting the Regulatory T Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
De-Hong Wu ◽  
Li Xu ◽  
Guan-Qun Xie ◽  
Yong-Sheng Fan ◽  
Jia Zhou
Keyword(s):  
Zingiber Officinale ◽  
Treg Cells ◽  
Chinese Herbs ◽  
Control Group ◽  
Antibody Array ◽  
Increasing Trend ◽  
Cytokine Antibody Array ◽  
Spleen Lymphocytes ◽  
Heat Syndrome ◽  
Immunosuppressive Cytokines

Heat syndrome is a folk saying in China, which is used to describe people with symptoms such as aphtha, oral ulcer, glossitis, swelling and aching of gingiva, and dry eye. Aconitum carmichaelii Debx. (A), Zingiber officinale Rosc. (Z), and Cinnamomum cassia Presl (C) are the representatives of pungent and hot Chinese herbs which may cause heat syndrome. In order to explore the mechanism of pungent herbs-induced heat syndrome, rats were treated with AZC extracts at different concentrations and at different time periods. A series of cytokines were determined using the cytokine antibody array; some immunosuppressive cytokines, including TGF-β, IL-10, and IL-35, significantly increased in AZC group as compared with control group. Higher mRNA expressions of Foxp3, TGF-β, IL-10, and IL-35 were found in the spleen and thymus of rats after treatment for 18 days based on RT-PCR. Flow cytometry result revealed that the percentage of CD4+CD25+ Treg cells and Foxp3+CD4+CD25+ Treg cells in spleen lymphocytes showed an increasing trend from the 3rd day to the 18th day after treatment with middle dose of AZC extracts. It is speculated that extracts of AZC herbs may affect the development of heat syndrome by influencing Treg cells and immunosuppressive cytokines.

Expression and Significance of Th17 Cells and Related Factors in Patients with Autoimmune Hepatitis

2019 ◽  
Vol 22 (4) ◽  
pp. 232-237 ◽  
Author(s):  
Jihong An
Keyword(s):  
Autoimmune Hepatitis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Treg Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Total Bilirubin ◽  
Study Group ◽  
Related Factors ◽  
Tnf Α

Objective: This study aims to investigate the expression and clinical significance of Th17 cells and related factors in peripheral blood of patients with Autoimmune Hepatitis (AIH). Methods: A retrospective selection of 100 patients with AIH were included as a study group, and 100 healthy volunteers in the outpatient clinic were selected as the control group. The levels of IL- 17, IL-6, IL-21 and TNF-α in peripheral blood of all subjects were detected by enzyme-linked immunosorbent assay and the frequency of Th17 cells and Treg cells was detected by flow cytometry. Results: Results showed that the study group had higher levels of serum total bilirubin (TBil), alkaline phosphatase (ALP), γ -glutamyltranspeptidase (γ-GT), immunoglobulin G (IgG), immunoglobulin M (IgM), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) than the control group, as well as higher levels of IL-17, IL-6, IL-21 and TNF-α in serum. The frequency of Th17 cells in peripheral blood was higher in the study group, while the frequency of Treg cells was lower. Also, serum IL-17, TNF-α levels and Th17 cells frequency were positively correlated with ALT and AST, whereas Treg cells frequency were negatively correlated with ALT and AST levels. Conclusion: Our finding demonstrates that Th17 cell frequency and their related factors IL-17 and TNF-α, are associated with liver damage, which might be used to monitor AIH disease severity.

scholarly journals The Effect of Zingiber officinale on the Spleen Tissue and Antibody Titer of Broiler Chickens

2019 ◽  
Vol 36 (01) ◽  
pp. 046-050
Author(s):  
Alireza Taghdisi ◽  
Sajjad Hejazi
Keyword(s):  
Broiler Chickens ◽  
Zingiber Officinale ◽  
Control Group ◽  
White Pulp ◽  
Broiler Chicks ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Serum Factors ◽  
Spleen Tissue ◽  
Significant Difference

Introduction Increasing the immune system's function of fighting infectious diseases is very important in the poultry industry. Ginger, scientifically known as Zingiber officinale, belongs to the Zingiberaceae family. The use of ginger in the diet of poultry increases serum levels of superoxide dismutase enzymes and glutathione peroxidase, which are considered to be important antioxidant enzymes. The main objective of the present study is to evaluate the effect of ginger on the spleen tissue of broiler chickens. Material and Methods The specimens comprised 2 groups of 20 Ross breed broiler chicks, for 42 days and were then, examined and tested. The diet was supplemented with 1 g/kg of ginger powder from the beginning of the rearing period. Blood samples of the chicks were randomly collected to measure the levels of hemagglutination (HI). The removed spleens were fixed with 10% formalin buffer. The specimens were cut in 5-micron diameters and stained with hematoxylin and eosin. Results and Conclusion There was a statistically significant difference in the mean of HI blood titers between the chicks in the growth period and final period groups (p < 0.05). The white-pulp tissue samples were more clearly seen in the treatment group than in the control group, and also, it was observed that the wall of the central artery of the white pulp was thicker in the ginger-treated group as compared with the control group. The nutritional value of ginger may vary. Thus, it is necessary to investigate the effect of this plant final on weight gain; the serum factors associated with the metabolic chart, and the response of the immune system to this plant.

scholarly journals Effects of Rapamycin Combined with Low Dose Prednisone in Patients with Chronic Immune Thrombocytopenia

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Jiaming Li ◽  
Zhaoyue Wang ◽  
Lan Dai ◽  
Lijuan Cao ◽  
Jian Su ◽  
...  
Keyword(s):  
Immune Thrombocytopenia ◽  
Low Dose ◽  
Group Versus ◽  
Treg Cells ◽  
Significant Rise ◽  
Control Group ◽  
Foxp3 Mrna ◽  
Experimental Group ◽  
Dose Prednisone ◽  
Chronic Immune Thrombocytopenia

We conducted this randomized trial to investigate the efficacy and safety of rapamycin treatment in adults with chronic immune thrombocytopenia (ITP). Eighty-eight patients were separated into the control (cyclosporine A plus prednisone) and experimental (rapamycin plus prednisone) groups. The CD4+CD25+CD127lowregulatory T (Treg) cells level, Foxp3 mRNA expression, and the relevant cytokines levels were measured before and after treatment. The overall response (OR) was similar in both groups (experimental group versus control group: 58% versus 62%,P=0.70). However, sustained response (SR) was more pronounced in the experimental group than in the control group (68% versus 39%,P<0.05). Both groups showed similar incidence of adverse events (7% versus 11%,P=0.51). As expected, the low pretreatment baseline level of Treg cells was seen in all patients (P<0.001); however, the experimental group experienced a significant rise in Treg cell level, and there was a strong correlation between the levels of Treg cells and TGF-beta after the treatment. In addition, the upregulation maintained a stable level during the follow-up phase. Thus, rapamycin plus low dose prednisone could provide a new promising option for therapy of ITP.

scholarly journals Yupingfeng Pulvis Regulates the Balance of T Cell Subsets in Asthma Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Zhigang Wang ◽  
Xueting Cai ◽  
Zhonghua Pang ◽  
Dawei Wang ◽  
Juan Ye ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Th17 Cells ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Treg Cells ◽  
T Cell Subsets ◽  
Control Group ◽  
Group Model ◽  
Therapy Effect ◽  
Tract Infections

Background. Yupingfeng Pulvis (HFBP) had played an active role in many diseases, especially respiratory tract infections. Exploring the possible prevention mechanism of HFBP may provide new ideas in clinical applications for this well-known herbal formula.Purpose. To study the possible mechanisms of therapy effect of HFBP on asthma mice via regulating the balance of Tregs and Th17 cells.Method. The female BALB/c mice were divided into five groups: control group, model group, prednisone (5.5 mg/kg) group, and 22 g/kg HFBP and 44 g/kg HFBP groups. Ovalbumin was used to make the asthma model of mice; the drug was ig administered daily after atomization for consecutive 15 d. The mice were killed after the last administration. The paraffin-embedded tissue sections of the lungs were stained by H&E. Tregs and Th17 cells in bronchoalveolar lavage fluid were detected by flow cytometry. IL-4, TGF-β, and TNF-αin the serum were detected by ELISA assay.Results. HFBP could alleviate the inflammation in the lung tissue of mice, decrease the proportion of Th17 cells, and increase the proportion of Treg cells in bronchoalveolar lavage fluid. HFBP could decrease IL-4 and TNF-αlevel and increase TGF-βlevel in blood.Conclusion. HFBP could treat the asthma through impacting the balance of Th17 cells and Treg cells as well as the levels of related inflammatory cytokines in asthma mice.

scholarly journals Potensi Ekstrak Jahe Merah (Zingiber Officinale Var. Rubrum) Sebagai Larvasida Alami Aedes Albopictus

2021 ◽  
Vol 8 (2) ◽  
pp. 106
Author(s):  
Qatrinida Qatrinida ◽  
Norfai Norfai ◽  
Kasman Kasman
Keyword(s):  
Aedes Albopictus ◽  
Zingiber Officinale ◽  
Lethal Concentration ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Lethal Time

DBD masih merupakan masalah kesehatan masyarakat utama di Indonesia. Upaya pencegahan yang dilakukan saat ini menggunakan insektisida berbahan dasar kimia yang dapat merusak lingkungan juga menimbulkan resistensi. Penggunaan larvasida alami dari tumbuhan dapat menjadi alternatif insektisida, salah satunya adalah jahe merah (Zingiber officinale var. rubrum) yang dapat dijadikan sebagai insektisida alami karena adanya kandungan senyawa aktif. Tujuan penelitian ini adalah untuk mengetahui tingkat kematian, perbedaan rerata kematian larva Aedes albopictus serta mengetahui Lethal Time dan Lethal Concentration. Jenis penelitian ini merupakan penelitian true eksperimental dengan posttest only control group design. Hasil penelitian menunjukkan bahwa kematian larva 100% lebih cepat pada konsenrasi 8% yaitu setelah 6 jam pengujian. Hasil Uji Kruskal Wallis untuk melihat perbedaan rerata kematian larva setiap jam kelompok perlakuan ekstrak jahe merah dengan kelompok kontrol menunjukkan terdapat perbedaan secara signifikan rerata kematian larva (0,000 < 0,05). Hasil uji probit Lethal Time menunjukkan bahwa diperlukan waktu 2,196 jam dalam mematikan 50% larva dan 4,788 jam dalam mematikan 99% larva dengan konsentrasi 8% sedangkan Lethal Concentration menunjukkan bahwa diperlukan konsentrasi sebesar sebesar 0,772% dalam mematikan 50% larva dan 1,973% dalam mematikan 99% larva setelah 9 jam pengukuran. Perlu dilakukan penelitian lebih lanjut untuk mengembangkan ekstrak jahe merah yang aman dan layak pakai namun tidak menghilangkan senyawa yang ada pada ekstrak jahe merah.

scholarly journals Mechanical Growth Factor Promotes FOXP3 Expression in Regulatory T Cells and Enhances Its Function in Treating Ankylosing Spondylitis

2021 ◽  
Author(s):  
Hongyu Qin ◽  
Shuangshuang Yuan ◽  
Hao Zeng ◽  
Hao Li ◽  
Jinsong Yang
Keyword(s):  
Ankylosing Spondylitis ◽  
Growth Factor ◽  
Bone Destruction ◽  
Foxp3 Expression ◽  
Treg Cells ◽  
Control Group ◽  
Related Factors ◽  
Cell Proliferation And Differentiation ◽  
Tnf Α ◽  
Proliferation And Differentiation

Abstract Objective: We aimed to verify whether mechanical growth factor (MGF) may be an effective target for treating ankylosing spondylitis. Methods: FOXP3 expression was measured in Treg cells from healthy male subjects administered MGF. A rat model of ankylosing spondylitis was established, and the level of ankylosing spondylitis-related factors (tumor necrosis factor [TNF]-α, interleukin [IL]-2, and IL-10) was measured. Results: We found that the proliferation and total number of Treg cells, as well as FOXP3 expression, significantly increased in the MGF-treated groups compared with those in the control. The level of inflammation, bone destruction, and new bone formation significantly decreased in rats treated with MGF compared with those in the control group. TNF-α expression significantly decreased, whereas the IL-2 and IL-10 levels significantly increased in the MGF group compared with those in the control. Conclusions: MGF may delay disease progression in ankylosing rats by inducing FOXP3 expression, promoting FOXP3+ Treg cell proliferation and differentiation, reducing TNF-α expression, and increasing IL-10 and IL-2 expression.

scholarly journals Profile of vascular and inflammatory aqueous humor cytokines in primary open angle glaucoma patients

2019 ◽  
Author(s):  
Ruyi Zhai ◽  
Huan Xu ◽  
Xinghuai Sun ◽  
Xiangmei Kong
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Antibody Array ◽  
Open Angle ◽  
Aqueous Samples ◽  
Age Related ◽  
Tnf Α ◽  
Cytokine Antibody Array ◽  
Angiopoietin 2

Abstract Purpose: This study was aimed to analyze the profile of vascular and inflammatory cytokines in aqueous humor of primary open angle glaucoma (POAG) patients. Methods: Aqueous humor samples were acquired from 6 POAG patients and 7 age-related cataract patients that constituted the control group. A human cytokine antibody array was used to detect 37 proteins related to inflammation and vascular regulation. Characteristics including age, gender, and intraocular pressure (IOP) of POAG and age-related cataract patients were compared. Correlation analyses between aqueous humor cytokines and characteristics were performed. Results: The cytokine antibody array results showed that the signal intensities of soluble vascular endothelial growth factor receptor 1 (sVEGFR-1), von Willebrand factor (vWF), tumor necrosis factor (TNF)-α, and angiopoietin-2 in POAG patients were significantly higher in aqueous samples compared to controls (p < 0.05, Student’s test or Mann-Whitney U-test). Among cytokines of all aqueous samples, preoperative IOP was positively associated with expression quantity of sVEGFR-1 (r = 0.647, p = 0.02) and VEGF-A (r = 0.602, p = 0.04) and negatively associated with plasminogen activator inhibitor 1 expression (r = -0.593, p = 0.04). Values for sVEGFR-1 were positively correlated with vWF (r = 0.646, p = 0.02) and TNF-α (r = 0.824, p < 0.001) with statistical significance. Conclusion: Pathogenesis of primary open angle glaucoma may be associated with both vascular dysfunction and inflammatory responses. The vascular factors may include endothelial dysfunction and damaged vascular permeability as indicated by abnormal expression of vWF, sVEGFR-1 and angiopoietin-2. Keywords: POAG, aqueous humor, cytokines, sVEGFR-1, vWF, angiopoietin-2

scholarly journals Increased Angiogenin Expression Correlates With Radiation Resistance and Predicts Poor Survival for Patients With Nasopharyngeal Carcinoma

2021 ◽  
Vol 12 ◽  
Author(s):  
Shan-Shan Guo ◽  
Yu-Jing Liang ◽  
Li-Ting Liu ◽  
Qiu-Yan Chen ◽  
Yue-Feng Wen ◽  
...  
Keyword(s):  
Prognostic Significance ◽  
Tumour Recurrence ◽  
Antibody Array ◽  
Therapeutic Approaches ◽  
Elevated Expression ◽  
Risk Patients ◽  
Cytokine Antibody Array ◽  
Patients Experience ◽  
Radio Sensitivity ◽  
Human Cytokine

Background: Despite the development of such multiple therapeutic approaches, approximately 20% patients experience recurrence. Identification of molecular markers for stratifying the different risks of tumour recurrence and progression is considered imperative.Methods: We used a RayBio Human Cytokine Antibody Array that simultaneously detected the levels of 297 proteins and profiled the conditioned medium of HONE1 cells and the radioresistant NPC cells HONE1-IR. We found Angiogenin(ANG) expression to be significantly increased in HONE1-IR and HONE1-IR cells exposed to 4-Gy X-ray radiation.Results: We investigated the expression of ANG in NPC tissues and explored its prognostic significance in patients with NPC. We found that ANG expression was increased in recurrent NPC tissues. Elevated expression of ANG induced radio-resistance in NPC cells, in addition to being significantly associated with shorter PFS, OS, and LRFS in patients with NPC. Multivariate analysis results revealed that ANG was an independent prognostic factor that predicted PFS, OS, and LRFS. Furthermore, a nomogram model was generated to predict OS in terms of ANG expression.Conclusion: Our results found the radioresistant function of ANG and proved the clinical prognostic significance of ANG, and the results could help predict radio-sensitivity and stratify high-risk patients or tumour recurrence.

Expression of Glucocorticoid Receptors in the Early Period After the Return of Spontaneous Circulation Among Patients Who Experienced Cardiac Arrest : A Retrospective Observational Study

2021 ◽  
Author(s):  
Yanan Yu ◽  
Ziren Tang ◽  
Jiabao Li ◽  
Miaorong Xie ◽  
Chenchen Hang ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Glucocorticoid Receptors ◽  
Early Period ◽  
Treg Cells ◽  
Spontaneous Circulation ◽  
Skewed Distribution ◽  
Control Group ◽  
Total Cortisol ◽  
Cell Counts ◽  
Healthy Control

Abstract Background: Rapid changes in glucocorticoid (GC) levels and adrenal insufficiency are related to the development of post-cardiac arrest syndrome. However, changes in glucocorticoid receptors (GR) have not been studied. Hence, this study aimed to investigate the association of early changes in GR and prognosis and immune response in patients who experienced cardiac arrest (CA). Methods: In this observational single-center case-control study, we enrolled patients who were in the early period of return of spontaneous circulation after CA and were admitted to the emergency department of the Beijing Chaoyang Hospital between October 2018 and October 2019. Age- and sex-matched healthy individuals were recruited for the control group after a physical examination.GR expression and cell counts of circulatory T and B lymphocytes, natural killer, and regulatory T (Treg) cells were assessed. Plasma total cortisol and adrenocorticotrophic hormone (ACTH) levels were tested. Since the data for total cortisol and ACTH levels had a skewed distribution, we compared our results with the natural logarithmic conversion values after adding 1 (ln [total cortisol+ 1], ln [ACTH+ 1]). Measurement data with a skewed distribution are expressed as medians (25th and 75th percentiles). The Mann–Whitney U test was used to compare variables between groups. The qualitative parameters in the 2 × 2 contingency table were used for analysis.Results: Overall, 85 patients who experienced CA and 40 healthy individuals were enrolled. All cell counts were lower and plasma total cortisol levels were higher (P<0.001) in patients who experienced CA than those in the healthy control group. GR expression in Treg cells and CD3+CD4+ T lymphocytes was not significantly different, but the mean fluorescence intensity and GR expression in other cells were lower in patients who experienced CA (P<0.05) than those in the healthy control group. ACTH levels did not show any difference. There were no significant differences between survivors and non-survivors. Conclusion: Our findings provide insights into GC sensitivity and immunosuppressive status in these patients, and a new perspective for GC targeted treatment.

scholarly journals Human menstrual blood-derived stem cells mitigate bleomycin-induced pulmonary fibrosis through anti-apoptosis and anti-inflammatory effects

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin Chen ◽  
Yi Wu ◽  
Yanling Wang ◽  
Lijun Chen ◽  
Wendi Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pulmonary Fibrosis ◽  
Therapeutic Potential ◽  
Lung Fibroblasts ◽  
Menstrual Blood ◽  
Inflammatory Factors ◽  
Mouse Lung ◽  
Control Group ◽  
Antibody Array

Abstract Background Idiopathic pulmonary fibrosis is a kind of diffuse interstitial lung disease, the pathogenesis of which is unclear, and there is currently a lack of good treatment to improve the survival rate. Human menstrual blood-derived mesenchymal stem cells (MenSCs) have shown great potential in regenerative medicine. This study aimed to explore the therapeutic potential of MenSCs for bleomycin-induced pulmonary fibrosis. Methods We investigated the transplantation of MenSCs in a pulmonary fibrosis mouse model induced by BLM. Mouse was divided into three groups: control group, BLM group, MenSC group. Twenty-one days after MenSC transplantation, we examined collagen content, pathological, fibrosis area in the lung tissue, and the level of inflammatory factors of serum. RNA sequence was used to examine the differential expressed gene between three groups. Transwell coculture experiments were further used to examine the function of MenSCs to MLE-12 cells and mouse lung fibroblasts (MLFs) in vitro. Results We observed that transplantation of MenSCs significantly improves pulmonary fibrosis mouse through evaluations of pathological lesions, collagen deposition, and inflammation. Transwell coculturing experiments showed that MenSCs suppress the proliferation and the differentiation of MLFs and inhibit the apoptosis of MLE-12 cells. Furthermore, antibody array results demonstrated that MenSCs inhibit the apoptosis of MLE-12 cells by suppressing the expression of inflammatory-related cytokines, including RANTES, Eotaxin, GM-CSF, MIP-1γ, MCP-5, CCL1, and GITR. Conclusions Collectively, our results suggested MenSCs have a great potential in the treatment of pulmonary fibrosis, and cytokines revealed in antibody array are expected to become the target of future therapy of MenSCs in clinical treatment of pulmonary fibrosis.

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