scholarly journals Evaluation of the Use of Different Solvents for Phytochemical Constituents, Antioxidants, and In Vitro Anti-Inflammatory Activities of Severinia buxifolia

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Dieu-Hien Truong ◽  
Dinh Hieu Nguyen ◽  
Nhat Thuy Anh Ta ◽  
Anh Vo Bui ◽  
Tuong Ha Do ◽  
...  
Keyword(s):  
Biological Activities ◽  
High Capacity ◽  
Inflammatory Activity ◽  
Chemical Components ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
The Impact ◽  
Promising Source ◽  
Severinia Buxifolia

Severinia buxifolia (Rutaceae) is a promising source of bioactive compounds since it has been traditionally used for the treatment of various diseases. The present study aimed at evaluating the impact of different solvents on extraction yields, phytochemical constituents and antioxidants, and in vitro anti-inflammatory activities of S. buxifolia. The results showed that the used solvents took an important role in the yield of extraction, the content of chemical components, and the tested biological activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w). The extract obtained from methanol exhibited high capacity of antioxidant (IC50 value of 16.99 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 28.86 μg/mL; antiproteinase activity: IC50 = 414.29 μg/mL; and membrane stabilization: IC50 = 319 μg/mL). The antioxidant activity of the S. buxifolia extract was found to be 3-fold higher than ascorbic acid, and the anti-inflammatory activity of S. buxifolia extract was comparable to aspirin. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy.

scholarly journals Inclusion Complexes of β and HPβ-Cyclodextrin with α, β Amyrin and In Vitro Anti-Inflammatory Activity

Biomolecules ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 241 ◽  
Author(s):  
Walter Ferreira da Silva Júnior ◽  
Danielle Lima Bezerra de Menezes ◽  
Luana Carvalho de Oliveira ◽  
Letícia Scherer Koester ◽  
Patrícia Danielle Oliveira de Almeida ◽  
...  
Keyword(s):  
Inclusion Complexes ◽  
Biological Activities ◽  
Physicochemical Characterization ◽  
Inflammatory Activity ◽  
Scanning Calorimetry ◽  
Promising Alternative ◽  
Wide Range ◽  
Natural Mixture ◽  
Anti Inflammatory

α, β amyrin (ABAM) is a natural mixture of pentacyclic triterpenes that has a wide range of biological activities. ABAM is isolated from the species of the Burseraceae family, in which the species Protium is commonly found in the Amazon region of Brazil. The aim of this work was to develop inclusion complexes (ICs) of ABAM and β-cyclodextrin (βCD) and hydroxypropyl-β-cyclodextrin (HPβCD) by physical mixing (PM) and kneading (KN) methods. Interactions between ABAM and the CD’s as well as the formation of ICs were confirmed by physicochemical characterization in the solid state by Fourier transform infrared (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetry (TG) and differential scanning calorimetry (DSC). Physicochemical characterization indicated the formation of ICs with both βCD and HPβCD. Such ICs were able to induce changes in the physicochemical properties of ABAM. In addition, the formation of ICs with cyclodextrins showed to be an effective and promising alternative to enhance the anti-inflammatory activity and safety of ABAM.

scholarly journals Intestinal Anti-Inflammatory Activity of Terpenes in Experimental Models (2010–2020): A Review

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5430
Author(s):  
Maria Elaine Araruna ◽  
Catarina Serafim ◽  
Edvaldo Alves Júnior ◽  
Clelia Hiruma-Lima ◽  
Margareth Diniz ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Biological Activities ◽  
Structural Diversity ◽  
Experimental Models ◽  
New Drugs ◽  
Inflammatory Activity ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Inflammatory bowel diseases (IBDs) refer to a group of disorders characterized by inflammation in the mucosa of the gastrointestinal tract, which mainly comprises Crohn’s disease (CD) and ulcerative colitis (UC). IBDs are characterized by inflammation of the intestinal mucosa, are highly debilitating, and are without a definitive cure. Their pathogenesis has not yet been fully elucidated; however, it is assumed that genetic, immunological, and environmental factors are involved. People affected by IBDs have relapses, and therapeutic regimens are not always able to keep symptoms in remission over the long term. Natural products emerge as an alternative for the development of new drugs; bioactive compounds are promising in the treatment of several disorders, among them those that affect the gastrointestinal tract, due to their wide structural diversity and biological activities. This review compiles 12 terpenes with intestinal anti-inflammatory activity evaluated in animal models and in vitro studies. The therapeutic approach to IBDs using terpenes acts basically to prevent oxidative stress, combat dysbiosis, restore intestinal permeability, and improve the inflammation process in different signaling pathways.

scholarly journals Biochemical properties and in vitro biological activities of extracts from seven folk medicinal plants growing wild in southern Tunisia

2019 ◽  
Author(s):  
Hajer Tlili ◽  
Najjaa Hanen ◽  
Abdelkerim Ben Arfa ◽  
Mohamed Neffati ◽  
Abdelbasset Boubakri ◽  
...  
Keyword(s):  
Plant Species ◽  
Proliferative Activity ◽  
Biological Activities ◽  
Folk Medicine ◽  
Biochemical Properties ◽  
Inflammatory Activity ◽  
Tumour Cell Line ◽  
Marrubium Vulgare ◽  
Anti Inflammatory

ABSTRACTRecently, much attention has been paid to the extracts obtained from plant species in order to analyse their biological activities. Due to the climate diversity in Tunisia, the traditional pharmacopoeia consists of a wide arsenal of medicinal plant species since long used in folk medicine, in foods as spices, and in aromatherapy. Although many of these species are nearly facing extinction, only a small proportion of them have been scientifically studied. Therefore, this study explores the biochemical properties of seven spontaneous plants, which were harvested in the arid Tunisian desert: Marrubium vulgare L., Rhus tripartita (Ucria) D.C., Thymelaea hirsute (L.) Endl., Plantago ovata Forsk., Herniaria fontanesii J. Gay., Ziziphus lotus and Hyoscyamus albus. Extracts from these plants were found to contain different types of secondary metabolites (polyphenols, flavonoids, condensed tannins, crude saponins, carotenoids and alkaloids) that are involved in important biological activities. The biological activity of the extracts obtained from each Tunisian plant was assessed: first of all, leukaemia and colon cancer cell lines (K-562 and CaCo-2 respectively) were treated with different concentrations of extracts, and then the anti-proliferative activity was observed. The results showed, in particular, how the plant extract from Rhus tripartita significantly inhibits cell proliferation, especially on the K-562 tumour cell line. Subsequently, the anti-inflammatory activity was also assessed, and the results showed that Herniaria fontanesii and Marrubium vulgare possess the highest activity in the group of analysed plants. Finally, the greatest acetylcholinesterase inhibitory effect was exhibited by the extract obtained from Rhus tripartita.In conclusion, all the Tunisian plants we analysed were shown to contain a remarkable amount of different bio-active compounds, thus confirming their involvement in several biological activities. Rhus tripartita and Ziziphus lotus were shown to be particularly effective in anti-proliferative activity, while Herniaria fontanesii were shown to have the best anti-inflammatory activity.

scholarly journals Investigation of Photoprotective, Anti-Inflammatory, Antioxidant Capacities and LC–ESI–MS Phenolic Profile of Astragalus gombiformis Pomel

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1937
Author(s):  
Sabrina Lekmine ◽  
Samira Boussekine ◽  
Salah Akkal ◽  
Antonio Ignacio Martín-García ◽  
Ali Boumegoura ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Antioxidant Properties ◽  
High Capacity ◽  
Inflammatory Activity ◽  
Protection Factor ◽  
Esi Ms ◽  
Phenolic Constituents ◽  
Anti Inflammatory ◽  
Skin Conditions

Plant-derived compounds have recently been gaining popularity as skincare factors due to their ability to absorb ultraviolet radiations and their anti-inflammatory, and antioxidant properties. In this light, this work aimed to evaluate in vitro the pharmacological activities of the butanolic extract prepared from the aerial parts of Astragalus gombiformis Pomel, an endemic species to southern Algeria. The sun protection factor was used to assess the photoprotective effect (SPF), the protein denaturation method to determine the anti-inflammatory activity, and brine shrimp nauplii and OxHLIA assay, respectively, to assess the cytotoxicity and antioxidant capacity of A. gombiformis. In addition, LC–ESI–MS analysis was employed for the characterization of the phenolic constituents of A. gombiformis. The results showed that A. gombiformis had high capacity for absorbing UV radiations with an SPF of 37.78 ± 0.85 and significant anti-inflammatory activity with a percentage inhibition of 75.38% which is close to that of diclofenac and ketoprofen. In addition, A. gombiformis was found to have effective cytotoxicity against Artemia nauplii with a DC50 value of about 44.7 µg/mL, but a weak hemolytic effect against human erythrocytes. LC–ESI–MS results detected the presence of 17 phenolic compounds with a predominance of cirsiliol, silymarin, quercitrin (quercetin-3-O-rhamnoside), and kaempferol. Taken together, these results suggest that A. gombiformis extract could be used as a skincare agent in cosmetic formulations, providing excellent antioxidant and anti-inflammatory protection, allowing the treatment of skin conditions, as well as a pharmaceutical agent with multidimensional applications.

scholarly journals Phytochemical Evaluation and Anti-Inflammatory Activity of Ethanolic Extract of Calotropis procera Leaves

2020 ◽  
Vol 11 (1) ◽  
pp. 1-6
Author(s):  
Naveed Aslam Dogar ◽  
Hamza Shahid ◽  
Hafiz Usama Shaukat ◽  
M. Abubakar Khan ◽  
Farooq Saleem
Keyword(s):  
Inflammatory Diseases ◽  
Biological Activities ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Calotropis Procera ◽  
Anti Cancer ◽  
Ethanolic Extraction ◽  
Anti Inflammatory

Background: Medicinal plants have been used since centuries to cure various diseases. There is a huge potential to investigate the medicinal impacts of different parts of plants. Roots, stem, leaves and fruits of Calotropis procera are known for their biological activities. Calotropis procera plant shows multiple pharmacological activities like anti-cancer, anti-microbial, antioxidant, anti-malarial, hepatoprotective and anti-diabetic activities. Objectives: The objective of the current research was ethanolic extraction of Calotropis procera leaves and to study the phytochemistry and anti-inflammatory activity. Methodology: In this effort, we used the extract of Calotropis procera leaves for detection of phytochemicals and anti-inflammatory activity in vitro by hypotonicity induced hemolysis on 2% HRBC suspension, using UV-Vis spectrophotometer. Results: Phytochemicals like alkaloids, terpenoids, and flavonoids were present in large amount while tannins, saponins, steroids and cardiac glycosides were in small amount, whereas phlobatannins and anthraquinone were not detected. The potential of the ethanolic extract of Calotropis procera leaves was compared with Diclofenac sodium (100μl/ml, 200μl/ml). The leaves extract of Calotropis procera (100, 200, 300, 400, 500μl/ml each) showed significant anti-inflammatory activity by hypotonicity induced hemolysis on 2% HRBC suspension. Conclusion: The Calotropis procera leaves have potential to cure inflammatory diseases and can be used as anti-inflammatory medicine and analgesic.

scholarly journals EVALUATION OF THE USE OF DIFFERENT SOLVENTS FOR PHYTOCHEMICAL CONSTITUENTS AND ANTIOXIDANTS ACTIVITY OF THE LEAVES OF MURRAYA KOENIGII (LINN.) SPRENG. (RUTACEAE)

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Aisha Idris Ali ◽  
Virginia Paul ◽  
Amit Chattree ◽  
Ranu Prasad ◽  
Ajit Paul ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Chemical Components ◽  
Murraya Koenigii ◽  
Antioxidants Activity ◽  
Wide Range ◽  
Extraction Solvents ◽  
Phytochemical Constituents ◽  
The Impact ◽  
Promising Source

Murraya koenigii (Rutaceae) is a promising source of bioactive compounds since the leaves of this plant has been traditionally used extensively in the Indian Ayurvedic system of medicine for the treatment of a wide range of diseases and disorders. Although the pharmacological effect of the plant’s bioactive compounds has been extensively studied, however, study on the effect of using different extraction solvents to extract these bioactive componentsis scarce. The aim of the present study was to evaluate the impact of different solvents on extraction yields, phytochemical constituents and antioxidants activity of dehydrated Murrayakoenigi leaves. The results showed that the used solvents play an important role in the yield of extraction and the content of chemical components. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (5.70%) as well as the highest content of phenolic (27.2 mg GAE/g DW) and flavonoid (15.55 mg QE/g DW). The extract obtained from methanol exhibited highest antioxidant scavenging activity (93%), (using 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical scavenging assay), and the antioxidant activity of Murraya koenigi leaves extract was found to be higher than ascorbic acid. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants constituents from Murraya koenigi leaves for utilization in pharmacognosy. To best of our knowledge this is the first report that directly compares these 4 extraction solvents for the extraction of bioactive components from Murraya koenigi leaves.

Legume Proteins as a Promising Source of Anti-Inflammatory Peptides

2019 ◽  
Vol 20 (12) ◽  
pp. 1204-1217 ◽  
Author(s):  
Aline Reyes-Díaz ◽  
Carmen Lizette Del-Toro-Sánchez ◽  
José Carlos Rodríguez-Figueroa ◽  
Santiago Valdéz-Hurtado ◽  
Francisco Javier Wong-Corral ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bioactive Peptides ◽  
Inflammatory Activity ◽  
In Vivo Studies ◽  
Cytokines And Chemokines ◽  
Legume Proteins ◽  
Anti Inflammatory ◽  
Promising Source

Legume proteins are precursors of bioactive components, such as peptides. In the present paper, different types of legume as sources of bioactive peptides and hydrolysates are considered and discussed based on their anti-inflammatory effect. Peptides with anti-inflammatory activity were included from in vitro and in vivo studies. Current strategies for obtaining bioactive peptides, as well as their structure and impact on health, were also reviewed. It was discovered that peptides derived from legume protein, mainly soybean and bean, can regulate several inflammatory markers, which include prostaglandin E2 (PGE2), nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX- 2), cytokines, and chemokines. So far, lunasin, VPY and γ-glutamyl peptides have been identified with anti-inflammatory activity but their mechanisms have not been fully elucidated. Furthermore, it is necessary to gather more information about hydrolysates containing peptides and single peptides with antiinflammatory activity. Considering the wide diversity, legume may be promising components to produce peptides efficient to ameliorate inflammatory disorders.

Anti-inflammatory Plant Polyphenolics and Cellular Action Mechanisms

2020 ◽  
Vol 16 (6) ◽  
pp. 809-817
Author(s):  
Reyaz H. Mir ◽  
Mubashir H. Masoodi
Keyword(s):  
Biological Activities ◽  
Wide Distribution ◽  
Research Literature ◽  
Inflammatory Activity ◽  
Systemic Review ◽  
Bibliographic Databases ◽  
Cellular Mechanisms ◽  
Anti Inflammatory

Background: Polyphenolics, a group of natural substances with a wide distribution in the plant kingdom present a great diversity of biological activities such as anti-oxidative, anti-inflammatory, anti-mutagenic, anti-carcinogenic, modulation of enzyme activity, prevention of CHD, etc. The objective of this review was to describe the relevant aspects of polyphenolics, reporting the different known groups, the probable mechanisms by which they act as anti-inflammatory agents. An attempt was also made to enumerate the possible leads e.g. curcumin, resveratrol, baicalein for further development. Methods: For peer-reviewed research literature we undertook a structured search of bibliographic databases using a focused review question. The quality of retrieved papers was appraised using standard tools. The systemic review consists of research using scientific databases such as PubMed, Scopus, Science Direct, and Google Scholar. Research: Compounds like Quercetin, luteolin, apigenin, fisetin, wogonin, and baicalein showed antiinflammatory activity in-vitro through different cellular mechanisms and these were also reported to possess significant anti-inflammatory activity in animal models of inflammation. Conclusion: It is evident that polyphenolic compounds such as flavonoids, lignans, phloroglucinols, stilbenes, diarylheptanoids, quinones, and phenylpropanoids exhibited significant anti-inflammatory activity in-vivo as well as in-vitro. Although, these active compounds are not drugs per se, however, they deserve further investigation as potential candidates for anti-inflammatory drug development through preclinical and clinical studies.

scholarly journals Polyphenolic content, Antioxidant and Anti-inflammatory activities of Melon (Cucumis melo L. var. inodorus) Seeds

2020 ◽  
Vol 10 (2-s) ◽  
pp. 22-26
Author(s):  
Amel Bouaziz ◽  
Saliha Djidel ◽  
Assia Bentaher ◽  
Seddik Khennouf
Keyword(s):  
Antioxidant Activity ◽  
Cucumis Melo ◽  
Inflammatory Activity ◽  
Food Ingredients ◽  
Dry Extract ◽  
Anti Inflammatory ◽  
Cucumis Melo L ◽  
Promising Source ◽  
Β Carotene

The aim of this study was to estimate the content of polyphenols, flavonoids and tannins compounds and to evaluate the antioxidant activity and  the  in vitro anti-inflammatory  of    the ethanolic extract of  melon (Cucumis melo L.  var inodorus) seeds. The Folin-Ciocalteau and AlCl3 methods were applied in order to quantify the polyphenolic and flavonoids contents, respectively. However, DPPH and  β-carotene bleaching  method were applied  to evaluate the  in vitro antioxidant activity. The polyphenolic  and flavonoids contents  of    seeds  extract  were  found to be 37,10 ± 1,11 mg GAE/g of dry extract and 0,43 ± 0,27 mg QE/ g  of dry extract, respectively. Whereas the amount of tannins was  48, 30 ±  0,90 mg TAE/ g of dry extract. The scavenger effect of  seeds extract against DPPH radicals showed IC50 value of  4,13 ± 0,07 mg/ ml. The β-carotene bleaching assay indicated a strong inhibition percentage of the lipid peroxidation with value of 69,47 %. The in vitro anti-inflammatory activity was evaluated using the inhibition of   proteins denaturation revealed that the seeds extract had a moderate anti-inflammatory activity which is estimated at 49,09 % compared to the aspirin (97,73  %) at 1 mg/ ml.  Finally, melon seeds may be considered as a promising source of natural antioxidants which possess remarkable therapeutic action as inflammatory activity and may serve as food ingredients. Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Oxidative stress,  Anti-inflammatory activity.

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