scholarly journals Retracted: Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons

2019 ◽  
Vol 2019 ◽  
pp. 1-1
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cholinergic Neurons

scholarly journals Methyl 3,4-Dihydroxybenzoate Induces Neural Stem Cells to Differentiate Into Cholinergic Neurons in vitro

2018 ◽  
Vol 12 ◽  
Author(s):  
Jun-Ping Pan ◽  
Yang Hu ◽  
Jia-Hui Wang ◽  
Yi-Rong Xin ◽  
Jun-Xing Jiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cholinergic Neurons

Human Neural Stem Cells Genetically Modified to Express Human Nerve Growth Factor (NGF) Gene Restore Cognition in the Mouse with Ibotenic Acid-Induced Cognitive Dysfunction

2012 ◽  
Vol 21 (11) ◽  
pp. 2487-2496 ◽  
Author(s):  
Hong J. Lee ◽  
In J. Lim ◽  
Seung W. Park ◽  
Yun B. Kim ◽  
Yong Ko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nerve Growth Factor ◽  
Cognitive Function ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Cholinergic Neurons ◽  
Ibotenic Acid ◽  
Learning Deficit ◽  
Human Neural Stem Cells ◽  
The Brain

Alzheimer's disease (AD) is characterized by degeneration and loss of neurons and synapses throughout the brain, causing the progressive decline in cognitive function leading to dementia. No effective treatment is currently available. Nerve growth factor (NGF) therapy has been proposed as a potential treatment of preventing degeneration of basal forebrain cholinergic neurons in AD. In a previous study, AD patient's own fibroblasts genetically modified to produce NGF were transplanted directly into the brain and protected cholinergic neurons from degeneration and improved cognitive function in AD patients. In the present study, human neural stem cells (NSCs) are used in place of fibroblasts to deliver NGF in ibotenic acid-induced learning-deficit rats. Intrahippocampal injection of ibotenic acid caused severe neuronal loss, resulting in learning and memory deficit. NGF protein released by F3.NGF human NSCs in culture medium is 10-fold over the control F3 naive NSCs at 1.2 μg/106 cells/day. Overexpression of NGF in F3.NGF cells induced improved survival of NSCs from cytotoxic agents H2O2, Aβ, or ibotenic acid in vitro. Intrahippocampal transplantation of F3.NGF cells was found to express NGF and fully improved the learning and memory function of ibotenic acid-challenged animals. Transplanted F3.NGF cells were found all over the brain and differentiated into neurons and astrocytes. The present study demonstrates that human NSCs overexpressing NGF improve cognitive function of learning-deficit model mice.

Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat

10.1038/nn974 ◽  
2002 ◽  
Vol 5 (12) ◽  
pp. 1271-1278 ◽  
Author(s):  
Ping Wu ◽  
Yevgeniya I. Tarasenko ◽  
Yanping Gu ◽  
Li-Yen M. Huang ◽  
Richard E. Coggeshall ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cholinergic Neurons ◽  
Human Neural Stem Cells ◽  
Adult Rat

Neural stem cells: isolation and differentiation into cholinergic neurons

Neuroreport ◽  
2006 ◽  
Vol 17 (13) ◽  
pp. 1433-1436 ◽  
Author(s):  
Tim Ting Hua Wang ◽  
Ai-Hong Jing ◽  
Xiang-Ying Luo ◽  
Ming Li ◽  
Yan Kang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cholinergic Neurons

scholarly journals Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Limin Wang ◽  
Feng He ◽  
Zhuoyuan Zhong ◽  
Ruiyan Lv ◽  
Songhua Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cholinergic Neurons ◽  
Plasmid Vector ◽  
Empty Plasmid ◽  
Enhanced Expression ◽  
Mature Neurons ◽  
Cholinergic Cell ◽  
Stimulation Of

Neurotrophic tyrosine kinase type 1 (NTRK1) plays critical roles in proliferation, differentiation, and survival of cholinergic neurons; however, it remains unknown whether enhanced expression of NTRK1 in neural stem cells (NSCs) can promote their differentiation into mature neurons. In this study, a plasmid encoding the rat NTRK1 gene was constructed and transfected into C17.2 mouse neural stem cells (NSCs). NTRK1 overexpression in C17.2 cells was confirmed by western blot. The NSCs overexpressing NTRK1 and the C17.2 NSCs transfected by an empty plasmid vector were treated with or without 100 ng/mL nerve growth factor (NGF) for 7 days. Expression of the cholinergic cell marker, choline acetyltransferase (ChAT), was detected by florescent immunocytochemistry (ICC). In the presence of NGF induction, the NSCs overexpressing NTRK1 differentiated into ChAT-immunopositive cells at 3-fold higher than the NSCs transfected by the plasmid vector (26% versus 9%,P<0.05). The data suggest that elevated NTRK1 expression increases differentiation of NSCs into cholinergic neurons under stimulation of NGF. The approach also represents an efficient strategy for generation of cholinergic neurons.

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