scholarly journals Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Shinya Takahashi ◽  
Masaki Yoshida ◽  
Makoto M. Watanabe ◽  
Hiroko Isoda
Keyword(s):  
Proinflammatory Cytokine ◽  
Tumor Necrosis ◽  
Inflammatory Responses ◽  
Ethanol Extract ◽  
No Production ◽  
Murine Macrophage ◽  
Macrophage Cells ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Necrosis Factor

Aurantiochytrium limacinum 4W-1b (AL4W-1b) is a newly discovered microalgal strain with unique features. In the present study, we investigated the effects of ethanol extracts of AL4W-1b on lipopolysaccharide- (LPS-) induced inflammatory responses in RAW264 murine macrophage cells. Pretreatment of RAW264 cells with the AL4W-1b extract significantly reduced the production of LPS-induced nitric oxide (NO) and the expression of proinflammatory cytokine genes, including tumor necrosis factor α, interleukin- (IL-) 1β, and IL-6. Treatment with the AL4W-1b extract also decreased the production of IL-1β and IL-6. These results suggest that AL4W-1b might have anti-inflammatory effects in RAW264 cells. The NF-κB inhibitor, BAY 11-7082, synergistically prevented LPS-induced NO production after pretreatment with the AL4W-1b extract. Thus, the AL4W-1b extract may affect not only the NF-κB pathway but also other inflammatory pathways. To the best of our knowledge, this is the first study to report the anti-inflammatory effects of AL4W-1b extract and its mechanism of action in LPS-stimulated murine macrophage cells.

scholarly journals Chemical Composition and Anti-inflammatory Activity of Chamaecyparis obtusa f. formosana Wood Essential Oil from Taiwan

2014 ◽  
Vol 9 (5) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Tzu-Chao Chien ◽  
Sheng-Fong Lo ◽  
Chen-Lung Ho
Keyword(s):  
Essential Oil ◽  
Tumor Necrosis ◽  
Chamaecyparis Obtusa ◽  
Composition Analysis ◽  
Murine Macrophage ◽  
Tnf Α ◽  
Cellular Signal ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Necrosis Factor

Chamaecyparis obtusa Siebold & Zucc. f. formosana Hayata (Cupressaceae) is one of the treasured conifers in Taiwan. In this study, the wood essential oil of the species was extracted and its anti-inflammatory bioactivities analyzed. The composition analysis of the essential oil yielded a total of 57 compounds with α-terpineol (19.4%), τ-muurolol (16.9%), borneol (16%), and α-cadinol (10.9%) predominating. The anti-inflammatory assays of the essential oil suggest that C. obtusa f. formosana wood oil has no cytotoxicity, and was capable of inhibiting the expressions of tumor necrosis factor (TNF-α), IL-6, IL-1, pro IL-1β, NO, iNOS, and NLRP3 in murine macrophage cells. It was unable, however, to inhibit the expression of COX-2. Further investigation of the effect of the essential oil on the cellular signal transduction indicated that it was capable of inhibiting ERK1/2, JNK1/2 and p38; it also showed excellent inhibition against ROS. Thus, the overall results indicated that C. obtusa f. formosana wood oil had very good anti-inflammatory efficacies.

Anti-inflammatory Activity of Herbal Medicines: Inhibition of Nitric Oxide Production and Tumor Necrosis Factor-α Secretion in an Activated Macrophage-like Cell Line

2005 ◽  
Vol 33 (03) ◽  
pp. 415-424 ◽  
Author(s):  
Eunkyue Park ◽  
Susan Kum ◽  
Chuanhua Wang ◽  
Seung Yong Park ◽  
Bo Sook Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tumor Necrosis ◽  
No Production ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Tnf Α ◽  
Activated Macrophage ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Necrosis Factor

Houttuynia cordata Thunb. (HC), Glycyrrhiza uralensis Fischer (GU), Forsythia suspense (Thunb.) Vahl (FS), and Lonicera japonica Thunb. (LJ) are Chinese herbs known to possess anti-inflammatory properties. The effects of aqueous extracts of these herbs on the production of the pro-inflammatory mediators, nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) were examined in an activated macrophage-like cell line, RAW 264.7 cells. Aqueous extracts from FS at 0.0625–2.0 mg/ml inhibited in vitro production of NO and secretion of TNF-α in a dose-dependent manner. FS at 1.0–2.0 mg/ml and 0.125–2.0 mg/ml significantly inhibited NO production and TNF-α, respectively. An extract of LJ demonstrated potent inhibition of both NO production and TNF-α secretion in a dose-dependent manner. An aqueous extract from HC inhibited NO production in a dose-dependent manner, but minimally (approximately 30%) inhibited TNF-α secretion at 0.0625 and 0.125 mg/ml. In contrast, an aqueous extract of GU had a minimal effect on both the production of NO and the secretion of TNF-α. Viability of cells at all concentrations studied was unaffected as determined by MTT cytotoxicity assay and trypan blue dye exclusion. These results suggest that aqueous extracts from FS, LJ and HC have anti-inflammatory actions as measured by inhibition of NO production and/or TNF-α secretion.

scholarly journals Metabolomic Screening of Anti-Inflammatory Compounds from the Leaves of Actinidia arguta (Hardy Kiwi)

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 47 ◽  
Author(s):  
Gyoung-Deuck Kim ◽  
Jin Lee ◽  
Joong-Hyuck Auh
Keyword(s):  
Nitric Oxide ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Reversed Phase ◽  
No Production ◽  
Actinidia Arguta ◽  
Bioassay Guided Fractionation ◽  
Anti Inflammatory ◽  
Ethanol Extracts

The metabolomic screening of potential anti-inflammatory compounds in the leaves of Actinidia arguta was performed by using LC-MS/MS. Ethanol extracts were prepared, and the anti-inflammatory effects were investigated based on nitric oxide (NO) synthesis and inducible nitric oxide synthase expression in lipopolysaccharide-induced RAW 264.7 macrophages. The 75% ethanol extract showed the highest inhibitory effect on nitric oxide (NO) production, and it was further separated by in vitro bioassay-guided fractionation using preparative LC with reversed-phase column separation. Through multiple steps of fractionation, sub-fraction 1-3 was finally purified, and caffeic acid derivatives, such as caffeoylthreonic acid and danshensu (salvianic acid A), were successfully identified as key anti-inflammatory compounds by LC-MS/MS and metabolomics analyses. This is the first study identifying anti-inflammatory compounds in A. arguta (Actinidia arguta) leaves through bioassay-guided fractionation and metabolomics screening. Results of this study would be useful for the application of A. arguta leaves as a nutraceutical.

scholarly journals Comparisons of the Anti-Inflammatory Activity of Dendropanax morbifera LEV Leaf Extract Contents Based on the Collection Season and Concentration of Ethanol as an Extraction Solvent

2020 ◽  
Vol 10 (23) ◽  
pp. 8756
Author(s):  
Kyeong Jin Kim ◽  
Ji Sun Youn ◽  
Young-Jun Kim ◽  
Ji Yeon Kim
Keyword(s):  
Inflammatory Cytokines ◽  
Inflammatory Responses ◽  
Ethanol Extract ◽  
Mapk Signaling ◽  
Hot Water ◽  
Mitogen Activated Protein Kinase ◽  
Inflammatory Activity ◽  
Dendropanax Morbifera ◽  
Anti Inflammatory ◽  
Ethanol Extracts

This study aimed to compare the anti-inflammatory activity of 10-year-old Dendropanax morbifera LEV (DM) leaf extracts. The leaves were collected during different seasons (May, August, and November), and the extracts were prepared using different methods (hot water, 30% ethanol, or 60% ethanol). Lipopolysaccharide-stimulated RAW264.7 cells were treated with these extracts for 12 h. The anti-inflammatory effects were evaluated by measuring the production of nitrite; prostaglandin E2 (PGE2); and inflammatory cytokines such as interleukin (IL)-6, IL-1β, and tumor necrosis factor-alpha, in addition to the mRNA expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 and activation of the nuclear factor κB (NF-κB)/mitogen-activated protein kinase (MAPK) pathways. The amyrin and polyphenol compositions of the extracts were analyzed using a triple time-of-flight mass spectrometer and high-performance liquid chromatography. The 30% ethanol extract harvested in May and 60% ethanol extracts collected in August and November displayed the highest inhibitions of nitrite, PGE2, and inflammatory cytokines. The 60% ethanol extract harvested in August suppressed activation of the NF-κB and MAPK signaling pathways. The contents of amyrin and polyphenol compounds were highly dependent on the ethanol concentration used during each season. These results suggest that ethanol extracts of DM leaves may have the potential to regulate inflammatory responses.

Anti-inflammatory Activities of an Ethanol Extract ofEcklonia stoloniferain Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cells

2012 ◽  
Vol 60 (36) ◽  
pp. 9120-9129 ◽  
Author(s):  
Min-Sup Lee ◽  
Mi-Sung Kwon ◽  
Ji-Woong Choi ◽  
Taisun Shin ◽  
Hong Kyoon No ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cells ◽  
Anti Inflammatory

scholarly journals Antioxidant and Anti-Inflammatory Properties of Longan (Dimocarpus longanLour.) Pericarp

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Guan-Jhong Huang ◽  
Bor-Sen Wang ◽  
Wei-Chao Lin ◽  
Shyh-Shyun Huang ◽  
Chao-Ying Lee ◽  
...  
Keyword(s):  
Tumor Necrosis ◽  
Radical Scavenging ◽  
Water Extract ◽  
No Production ◽  
Paw Edema ◽  
Dependent Inhibition ◽  
Reducing Activity ◽  
Anti Inflammatory ◽  
Protection Activity ◽  
Necrosis Factor

This study examined the antioxidant and anti-inflammatory activities of the water extract of longan pericarp (WLP). The results showed that WLP exhibited radical scavenging, reducing activity and liposome protection activity. In addition, WLP also inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophages. Further, administration of WLP, in the range of 100–400 mg/kg, showed a concentration-dependent inhibition on paw edema development following carrageenan (Carr) treatment in mice. The anti-inflammatory effects of WLP may be related to NO and tumor necrosis factor (TNF-α) suppression and associated with the increase in the activities of antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. Overall, the results showed that WLP might serve as a natural antioxidant and inflammatory inhibitor.

scholarly journals Brassica juncea L. (Mustard) Extract Silver NanoParticles and Knocking off Oxidative Stress, ProInflammatory Cytokine and Reverse DNA Genotoxicity

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1650
Author(s):  
Sohair Aly Hassan ◽  
Ali Mohamed El Hagrassi ◽  
Olfat Hammam ◽  
Abdelmohsen M. Soliman ◽  
Essam Ezzeldin ◽  
...  
Keyword(s):  
Liver Failure ◽  
Proinflammatory Cytokine ◽  
Tumor Necrosis ◽  
Total Bilirubin ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Male Rats ◽  
Tnf Α ◽  
Liver Functions ◽  
Necrosis Factor

Detoxification is one of the main vital tasks performed by the liver. The purpose of this study was to investigate whether mustard in its normal or nanoparticles could confer a protective/therapeutic effect against TAA-induced acute liver failure in experimental animal models. Mustard ethanolic extract was analyzed by HPLC/MS. To induce liver failure, male rats were injected with 350 mg/kg bw TAA IP, then treated orally with a dose of 100 mg/kg for 15 d of mustard extract and its nanoform before and following induction. The levels of serum liver functions, total cholesterol (TCHo), total glyceride (TG), total bilirubin (TBIL), hepatic malonaldhyde (MDA) and nitric oxide (NO),glutathione (GSH), sodium oxide dismutase (SOD), as well as tumor necrosis factor (TNF-α,) and interleukin 6 (IL-6), were estimated. DNA genotoxicity and hepatic pathology, and immunohistologic (IHC) changes were assayed. The antioxidant content of Phenolic acids, flavonoids in mustard ethanolic extract substantially decreased the levels of ALT, AST, ALP and rehabilitated the histopathological alterations. In addition, nanoforms of mustard ethanol extract have notably increased the levels of GSH, SOD and significantly reduced the levels of MDA. The expression levels of TNF-α and IL-6 in serum and tissue were markedly downregulated. DNA genotoxicity was significantly reversed. Mustard introduced a protective and medicinal effect against TAA in both its forms.

Tumor Necrosis Factor Alpha Converting Enzyme (TACE) as Possible Therapeutic Target in SARS-CoV-2 Induced Acute Respiratory Distress Syndrome (ARDS)

2020 ◽  
Author(s):  
Joao Batista Junior
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Inflammatory Responses ◽  
Converting Enzyme ◽  
Necrosis Factor Alpha ◽  
Tace Inhibitors ◽  
Factor Alpha ◽  
Activity State ◽  
Necrosis Factor

<div>This study reveals, for the first time, that rosiglitazone and pioglitazone, two thiazolidinedione drugs already approved as therapeutic agents to treat type II diabetes, were found to bind favorably to tumor necrosis factor alpha converting enzyme catalytic site with highlighted binding features.</div><div><br></div>This study suggests that rosiglitazone and pioglitazone, acting as TACE inhibitors agents might avoid or attenuate the hyperexcitability proteolytic activity state of TACE, represent a new potential therapeutic approach to treat SARS-CoV-2 infection-associated severe systemic inflammatory responses observed among severely or critically ill SARS-CoV-2 patients and, consequently, to diminish severe inflammatory‐induced lung injury, ARDS development and death rates.<br><br>

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