scholarly journals Synthesis, Antimicrobial Properties, and Inhibition of Catalase Activity of 1,4-Naphtho- and Benzoquinone Derivatives Containing N-, S-, O-Substituted

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Semih Kurban ◽  
Nahide Gulsah Deniz ◽  
Cigdem Sayil ◽  
Mustafa Ozyurek ◽  
Kubilay Guclu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Catalase Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Significant Inhibition ◽  
Inhibition Activity ◽  
Low Concentrations ◽  
Test Culture ◽  
First Time

A series of new 1,4-naphtho- and benzoquinone derivatives possessing N-, S-, O-substituted groups which has not been reported yet has been synthesized from 2,3-dichloro-1,4-naphthoquinone 1 and 2,3,5,6-tetrachlorocyclohexa-2,5-diene-1,4-dione 15 involving a Michael addition. In the synthesized compounds, antimicrobial activity at low concentrations against Escherichia coli B-906, Staphylococcus aureus 209-P, and Mycobacterium luteum B-917 bacteria and Candida tenuis VKM Y-70 and Aspergillus niger F-1119 fungi in comparison with controls was identified. 2-Chloro-3-((2-(piperidin-1-yl)ethyl)amino)naphthalene-1,4-dione 3g and 2,5-dichloro-3-ethoxy-6-((2,4,6-trifluorophenyl)amino)cyclohexa-2,5-diene-1,4-dione 17 were the most potent, with a minimum inhibitory concentration value of 15.6 μg/mL against test-culture M. luteum and S. aureus, respectively. Furthermore, in this work, a catalase activity of benzo- and naphthoquinone derivatives was examined for the first time. The catalase activity of benzo- and naphthoquinone derivatives was determined, showing that compound 3g had significant inhibition activity for catalase enzyme.

scholarly journals Bio-Guided Fractionation of Prenylated Benzaldehyde Derivatives as Potent Antimicrobial and Antibiofilm from Ammi majus L. Fruits-Associated Aspergillus amstelodami

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4118 ◽  
Author(s):  
Noha Fathallah ◽  
Marwa M. Raafat ◽  
Marwa Y. Issa ◽  
Marwa M. Abdel-Aziz ◽  
Mokhtar Bishr ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Antibiofilm Activity ◽  
Moderate Activity ◽  
Ammi Majus ◽  
First Time

Ammi majus L.; Family Apiaceae; is a plant indigenous to Egypt. Its fruits contain bioactive compounds such as furanocoumarins and flavonoids of important biological activities. An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708) by morphology, microscopical characterization, and molecular identification. To our knowledge this is the first time an endophytic fungus has been isolated from the fruits. The antimicrobial activity of the Ammi majus ethanol fruits extract (AME) and fungal ethyl acetate extract (FEA) were investigated, where the FEA showed higher antimicrobial activity, against all the tested standard strains. Phytochemical investigation of the FEA extract yielded five prenylated benzaldehyde derivative compounds isolated for the first time from this species: Dihydroauroglaucin (1), tetrahydroauroglaucin (2), 2-(3,6-dihydroxyhepta-1,4-dien-1-yl)-3,6-dihydroxy-5-(dimethylallyl)benzaldehyde (3), isotetrahydroauroglaucin )4), and flavoglaucin (5). Structure elucidation was carried out using (1H- and 13C-NMR). Fractions and the major isolated compound 1 were evaluated for their antimicrobial and antibiofilm activity. Compound 1 showed high antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC) = 1.95 µg/mL, Streptococcus mutans (MIC = 1.95 µg/mL), and Staphylococcus aureus (MIC = 3.9 µg/mL). It exhibited high antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) = 7.81 µg/mL against Staphylococcus aureus and Escherichia coli biofilms and MBIC = 15.63 µg/mL against Streptococcus mutans and Candida albicans and moderate activity (MBIC = 31.25 µg/mL) against Pseudomonas aeruginosa biofilm. This reveals that dihydroauroglaucin, a prenylated benzaldehyde derivative, has a broad spectrum antimicrobial activity. In conclusion, it was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of Ammi majus may be due to the ability of its endophytic fungi to produce effective secondary metabolites.

scholarly journals Physicochemical Properties and Antimicrobial Activity of Evaporated Extract of Cow Dung Against Some Pathogens

2012 ◽  
Vol 5 (1) ◽  
pp. 135-141
Author(s):  
M. Waziri ◽  
J. S. Suleiman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Analytical Techniques ◽  
Food Additive ◽  
Cow Dung ◽  
Northern Nigeria ◽  
Elemental Contents ◽  
Mic Value

The evaporated extract of cow dung is traditionally used in Northern Nigeria and Cameroun as food additive and in treatment of infectious diseases. In this study, the cow dung ash extract was prepared and tested for some elemental contents as well as the antimicrobial activity against Cyanobacteria (C.bacteria), Staphylococcus aureus (S.aureus), Bacillus subtilis (B.subtilis) and Escherichia coli (E.coli) using different analytical techniques. The extract was highly basic with pH of 11.7 and the elements vary in the following decreasing order of concentration; K>Na>Mg>Ca>Fe>Al>Zn.  S. aureus was the most sensitive bacteria with minimum inhibitory concentration (MIC) value of 0.082 mg/mL while B. subtilis was the least sensitive with MIC value of 4.3 mg/mL. The result of this study indicate that the extract can supplement the dietary Na and K requirements for the users and supports the folkloric use of the extract in treatment of infections.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i1.11962        J. Sci. Res. 5 (1), 135-141 (2013)

Antimicrobial Activity of N,N'-bis(decylmethyl)- α,ω-alkanediamine Dioxides [1]

1983 ◽  
Vol 38 (1-2) ◽  
pp. 151-152 ◽  
Author(s):  
Ferdinand Devínsky ◽  
Ivan Lacko ◽  
Ludovít Krasnec ◽  
Dušan Mlynarčík
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration

Antimicrobial activity of N,N′-bis(decylmethyl)-α,ω-alkanediamine dioxides determined on Staphylococcus aureus, Escherichia coli, and Candida albicans is presented as minimal inhibitory concentration (MIC). The effect of the length of linking alkylene chain on this activity has been followed.

scholarly journals Antimicrobial Activity of Cultivable Endophytic and Rhizosphere Fungi Associated with “Mile-a-Minute,” Mikania cordata (Asteraceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Pavithra L. Jayatilake ◽  
Helani Munasinghe
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Pharmaceutical Products ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Disk Diffusion Method ◽  
Rhizosphere Fungi

Endophytic and rhizosphere fungi are understood to be aiding the host plant to overcome a range of biotic and abiotic stresses (nutrition depletion, droughts, etc.) hence, they remain to be reservoirs of plethora of natural products with immense use. Consequently, this investigation of endophytic and rhizosphere fungi isolated from Mikania cordata (a perennial vine that is well established in Sri Lanka) for their antimicrobial properties was performed with the aim of future derivation of potential beneficial pharmaceutical products. Leaves, twigs, and roots of M. cordata were utilized to isolate a total of 9 endophytic fungi out of which the highest amount (44%) accounted was from the twigs. A sample of the immediate layer of soil adhering to the root of M. cordata was utilized to isolate 15 rhizosphere fungi. Fusarium equiseti and Phoma medicaginis were endophytes that were identified based on colony and molecular characteristics. The broad spectrum of antimicrobial activity depicted by F. equiseti (MK517551) was found to be significantly greater (p≤0.05, inhibitory against Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) than P. medicaginis (MK517550) (inhibitory against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) as assessed using the Kirby-Bauer disk diffusion method. Trichoderma virens and Trichoderma asperellum were rhizospere fungi that exhibited remarkable antimicrobial properties against the test pathogens chosen for the study. T. asperellum indicated significantly greater bioactivity against all four bacterial pathogens and Candida albicans ATCC 10231 under study. The ranges of minimum inhibitory concentrations (MICs) of the fungi depicting antimicrobial properties were determined. The results obtained suggest that F. equiseti, P. medicaginis, T. asperellum, and T. virens of M. cordata harness bioprospective values as natural drug candidates. This is the first report on isolation and evaluation of the antimicrobial properties of endophytic and rhizosphere fungi of Mikania cordata.

scholarly journals Assessment of antimicrobial activity of Cola milleni seed and pulp extracts against Staphylococcus aureus, Escherichia coli and Penicillium notatum

2020 ◽  
Vol 12 (1) ◽  
pp. 71-75
Author(s):  
A.M. Aliyu ◽  
S.J. Oluwafemi ◽  
S. Kasim
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Bioactive Components ◽  
Zone Of Inhibition ◽  
Penicillium Notatum ◽  
Standard Techniques

All over the world, hundreds of plants have been identified based on researchers and experimental evidence as good sources of medicinal agents. The bioactive components (phytochemicals) of both the seeds and pulp of Cola milleni were extracted using ethanol as solvent. The bioactive components detected were alkaloids, tanins, saponins, cardiac glycosides, carbohydrates, sterols, resins and terpenes while Flavonoids, anthraquinones, anthracyanides and phenol were not detected for both the seed and pulps. Antimicrobial activity of the ethanol extract (Seed and pulp) against Staphylococcus aureus, Escherichia coli and Penicillium notatum was carried out using standard techniques. Staphylococcus aureus had the highest zone of inhibition for pulp having a range of 9.7mm±0.58mm - 19.7mm±2.52mm while Penicllium notatum had the least with 0.00mm. S.aureus also had the highest zone of inhibition range of 14.3mm±2.08mm - 21.3mm±1.53mm for the seed extract while penicillium had the least inhibition range of 5.0mm±1.00mm - 5.7mm±0.58. E.coli showed the highest minimum inhibitory concentration with ethanol extract of the pulp (160mg/ml) while penicillium notatum was not reactive. The minimum inhibitory concentration of seed against penillium notatum was the highest (160mg/ml) while staphylococcus aureus showed the lowest of 40mg/ml. The antimicrobial activity is as a result of the presence of phytochemicals detected, which suggest the use of the plant for the treatment of diseases caused by these organisms. Key words: Cola millenii, Phytochemical, Antimicrobial activity, Bacteria, Fungi

scholarly journals In vitro antibacterial effect of Euterpe oleracea Mart. and Theobroma grandiflorum hydroalcoholic extracts

2016 ◽  
Vol 21 (2) ◽  
Author(s):  
Lew Kan Sprenger ◽  
Elane Guerreiro Giese ◽  
Jeannie Nascimento Dos Santos ◽  
Marcelo Beltrão Molento
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Treatment ◽  
Significant Inhibition ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Euterpe Oleracea ◽  
Agar Disc ◽  
Euterpe Oleracea Mart

This study evaluated the in vitro antimicrobial activity of these species against strains of Gram-positive and Gram-negative bacteria. The hydroalcoholic extracts were prepared from dried leaves, pulp and seeds of E. oleracea Mart. and T. grandiflorum by continuous percolation with 70% ethyl alcohol. The antimicrobial activity was evaluated against four microorganisms by the agar disc diffusion method and the minimal inhibitory concentration (MIC) assay. The antimicrobial activity showed that the açai pulp and seeds possessed significant inhibition in Clostridium perfringens (320 and 640 MIC), Staphylococcus aureus (80 and 320 MIC) and Pseudomonas aeruginosa (640 and 2560 MIC). Cupuassu extracts showed no effect on any bacteria. The use of açai extract products can be a sustainable, viable and an accessible alternative for antimicrobial treatment. New studies should be conducted to determine better results for acai herbals and new formulations of cupuassu extracts.

scholarly journals Chemical Constituents from the Fruits ofForsythia suspensaand Their Antimicrobial Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ping-Chung Kuo ◽  
Guo-Feng Chen ◽  
Mei-Lin Yang ◽  
Ya-Hua Lin ◽  
Chi-Chung Peng
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Column Chromatography ◽  
Chemical Constituents ◽  
Phenylethanoid Glycosides ◽  
Natural Sources ◽  
Methanol Extracts ◽  
Lead Compounds ◽  
Forsythia Suspensa ◽  
First Time

Lignans and phenylethanoid glycosides purified fromForsythia suspensawere reported to display various bioactivities in the previous literature, including the antimicrobial activity. Therefore, the present research is aimed to purify and identify the chemical constituents of the methanol extracts of fruits ofF. suspensa. The methanol extracts of fruits ofF. suspensawere fractionated and further purified with the assistance of column chromatography to afford totally thirty-four compounds. Among these isolates, 3β-acetoxy-20α-hydroxyursan-28-oic acid (1) was reported from the natural sources for the first time. Some of the purified principles were subjected to the antimicrobial activity examinations againstEscherichia colito explore new natural lead compounds.

scholarly journals Some Biological Activities of Malaysian Leech Saliva Extract

2011 ◽  
Vol 12 (4) ◽  
Author(s):  
Abdualrahman M. Abdualkader ◽  
Ahmed Merzouk ◽  
Abbas Mohammed Ghawi ◽  
And Mohammed Alaama
Keyword(s):  
Antimicrobial Activity ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Anticoagulant Activity ◽  
Thrombin Time ◽  
Inhibition Activity ◽  
Saliva Concentration

Leeches were fed on the phagostimulatory solution through parafilm membrane. The satiated leeches were forced to regurgitate the solution by soaking them in an ice-container. The anticoagulant activity was ascertained using thrombin time assay (TT). The result revealed that the saliva concentration which increases TT by 100% (IC100) is 43.205µg/ml plasma. The antimicrobial activity of the saliva was tested against several bacterial spp. (E.coli, P.aeruginosa, B.cereus, Sal.typhi and S.aureus)  and fungi spp. (C.albicans and C.neoformans). It was found that saliva has an inhibition activity against Sal.typhi (minimal inhibitory concentration MIC 78.253µg/ml), S.aureus (MIC 78.253µg/ml) and E.coli (MIC 121.256µg/ml).ABSTRAK: Pacat-pacat diberi makan larutan phagostimulatory menerusi membran parafilem. Pacat-pacat yang kekenyangan itu dipaksa memuntahkan larutan tersebut dengan direndam di dalam bekas berisi ais. Aktiviti antigumpal ditentukan menggunakan cerakin masa trombin (TT). Keputusan menunjukkan kepekatan air liur pacat menyebabkan pertambahan TT sebanyak 100% (IC100) iaitu 43.205µg/ml plasma. Aktiviti antimikrob air liur telah diuji dengan pelbagai jenis bakteria (E.coli, P.aeruginosa, B.cereus, Sal.typhi dan S.aureus) dan pelbagai jenis kulat (C.albicans and C.neoformans). Didapati air liur menghasilkan aktiviti perencatan terhadap Sal.typhi (kepekatan perencat minima (Minimal inhibitory concentration - MIC) 78.253µg/ml), S.aureus (MIC 78.253µg/ml) dan E.coli (MIC 121.256µg/ml).

scholarly journals Antimicrobial activity of root canal filling materials

2021 ◽  
Vol 7 (1) ◽  
pp. 01-05
Author(s):  
Jikia Maia
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Antimicrobial Properties ◽  
Root Canal Filling ◽  
Root Canals ◽  
Filling Materials ◽  
Zinc Oxide Eugenol ◽  
Test Culture

The antimicrobial activity of filling materials was studied to improve the quality of treatment of complicated caries in vitro. Resorcin-formalin cement Resodont (“Latus”, Ukraine) - № 1; Endocort zinc oxide-eugenol cement (“Latus”, Ukraine) - No. 2; materials based on zinc oxide eugenol cement with dexamethasone: Endomethasone ivory (Septodont, France) - No. 3 and Endofil ("Produit Dentaires SA", Switzerland) - No. 4. To assess the antimicrobial activity of the studied materials, test microorganism strains were used: Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 6783, Candida albicans ATCC 885-653. As a result of the study, it was established that filling materials have different antimicrobial activity. Resodont has the greatest antimicrobial activity on the test culture. Endomethason is active in relation to the test cultures studied, inferior to Resodont in its antimicrobial properties. Endocort and Endofil have less antimicrobial activity than Resodont and Endomethason, but have approximately equal growth retardation rates. It should be noted that with respect to Staphylococcus aureus, Endocort was found to be almost 1.5 times more active than Endofil. The conducted studies confirm that the materials for filling of root canals by Resodont and Endocort (Latus, Ukraine) have a pronounced antibacterial effect. They are competitive with foreign sealers and are the materials of choice, having a significantly lower cost.

scholarly journals Isolation of Bifidobacterium from Infant’s Feces and Its Antimicrobial Activity

2020 ◽  
Vol 2 ◽  
pp. 00002
Author(s):  
Dyah Fitri Kusharyati ◽  
Pancrasia Maria Hendrati ◽  
Dini Ryandini ◽  
Tsani Abu Manshur ◽  
Meilany Ariati Dewi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Inhibition Activity ◽  
Antimicrobial Substance ◽  
Inhibitory Zone ◽  
E Coli

<p class="Abstract"><i>Bifidobacterium</i> is a group of Lactic Acid Bacteria (LAB) that commonly found in the gastrointestinal tract and vagina. LAB has many health benefits, such as produce an antimicrobial substance against a pathogen. This research aims to isolate <i>Bifidobacterium</i> from an infant’s feces and know its antimicrobial activity against <i>Escherichia coli</i> and <i>Candida albicans.</i> A total of 5 isolates <i>Bifidobacterium</i> spp. were isolated from the sample. <span lang="EN">The largest inhibitory activity against <i>E. coli</i> was shown by isolate Bb3F, with the inhibitory zone of 10.80 mm. While the largest inhibition activity against <i>C. albicans</i> was shown by isolate Bb1B and Bb3F with the inhibitory zone of 9.70 mm.</span><o:p></o:p></p>

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