scholarly journals Salivary MicroRNAs for Early Detection of Head and Neck Squamous Cell Carcinoma: A Case-Control Study in the High Altitude Mestizo Ecuadorian Population

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Carolina Salazar-Ruales ◽  
Jessica-Viviana Arguello ◽  
Andrés López-Cortés ◽  
Alejandro Cabrera-Andrade ◽  
Jennyfer M. García-Cárdenas ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
High Altitude ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Expression Profiles ◽  
Neck Squamous Cell Carcinoma ◽  
Altered Expression ◽  
Mortality And Morbidity ◽  
Tumor Stages

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer with the highest incidence worldwide. HNSCC is often diagnosed at advanced stages, incurring significant high mortality and morbidity. The use of saliva, as a noninvasive tool for the diagnosis of cancer, has recently increased. Salivary microRNAs (miRNAs) have emerged as a promising molecular tool for early diagnosis of HNSCC. The aim was to identify the differential expression of salivary miRNAs associated with HNSCC in the high altitude mestizo Ecuadorian population. Using PCR Arrays, miR-122-5p, miR-92a-3p, miR-124-3p, miR-205-5p, and miR-146a-5p were found as the most representative ones. Subsequently, miRNAs expression was confirmed in saliva samples from 108 cases and 108 controls. miR-122-5p, miR-92a-3p, miR-124-3p, and miR-146a-5p showed significant statistical difference between cases and controls with areas under the curve (AUC) of 0.73 (p < 0.001), 0.70 (p < 0.001), 0.71 (p = 0.002), and 0.66 (p = 0.008), respectively. miRNAs were also deregulated in between HNSCC localizations. A differentiated expression of miR-122-5p between oral cancer and oropharynx cancer (AUC of 0.96 p = 0.01) was found: miR-124-3p between larynx and pharynx (AUC = 0.97, p < 0.01) and miR-146a-5p between larynx, oropharynx, and oral cavity (AUC = 0.96, p = 0.01). Moreover, miR-122-5p, miR-124-3p, miR-205-5p, and miR-146a-5p could differentiate between HPV+ and HPV- (p=0.004). Finally, the expression profiles of the five miRNAs were evaluated to discriminate HNSCC patient’s tumor stages (TNM 2-4). miR-122-5p differentiates TNM 2 and 3 (p = 0.002, AUC = 0.92), miR-124-3p TNM 2, 3, and 4 (p < 0.001, AUC = 98), miR-146a-5p TNM 2 and 3 (p < 0.001, AUC = 0.97), and miR-92a-3p TNM 3 (p < 0.001, AUC = 0.99). Taken together, these findings show that altered expression of miRNAs could be used as biomarkers for HNSCC diagnosis in the high altitude mestizo Ecuadorian population.

scholarly journals MicroRNA expression profiles of cancer stem cells in head and neck squamous cell carcinoma

2015 ◽  
Vol 47 (4) ◽  
pp. 1249-1256 ◽  
Author(s):  
KAZUYA YATA ◽  
LEVENT BEKIR BEDER ◽  
SHUNJI TAMAGAWA ◽  
MUNEKI HOTOMI ◽  
YOSHIHIKO HIROHASHI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Squamous Cell Carcinoma ◽  
Cancer Stem Cells ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Expression Profiles ◽  
Microrna Expression ◽  
Neck Squamous Cell Carcinoma

scholarly journals miRNA expression profiles in head and neck squamous cell carcinoma and adjacent normal tissue

Head & Neck ◽  
2009 ◽  
Vol 31 (5) ◽  
pp. 642-654 ◽  
Author(s):  
Latha Ramdas ◽  
Uma Giri ◽  
Cheryl L. Ashorn ◽  
Kevin R. Coombes ◽  
Adel El-Naggar ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Mirna Expression ◽  
Normal Tissue ◽  
Expression Profiles ◽  
Neck Squamous Cell Carcinoma ◽  
Adjacent Normal Tissue ◽  
Mirna Expression Profiles

scholarly journals Identification and Confirmation of the miR-30 Family as a Potential Central Player in Tobacco-Related Head and Neck Squamous Cell Carcinoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Tingting Zhang ◽  
Xueqin Zhu ◽  
Qiang Sun ◽  
Xing Qin ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Expression Profiles ◽  
Family Members ◽  
Neck Squamous Cell Carcinoma ◽  
Migration And Invasion ◽  
Control Group

Constituents of tobacco that can cause DNA adduct formation and oxidative stress are implicated in the development of head and neck squamous cell carcinoma (HNSCC). However, there are few studies on the mechanism(s) that underlie tobacco-associated HNSCC. Here, we used a model in which tumors were induced in rats using 4-nitroquinoline 1-oxide (4NQO), which mimicked tobacco-related HNSCC, and analyzed the expression profiles of microRNAs and mRNAs. Our results indicated that 57 miRNAs and 474 mRNA/EST transcripts exhibited differential expression profiles between tumor and normal tongue tissues. In tumor tissue, the expression levels of rno-miR-30 family members (rno-miR-30a, rno-miR-30a-3p, rno-miR-30b-5p, rno-miR-30c, rno-miR-30d, rno-miR-30e and rno-miR-30e-3p) were only 8% to 37% of those in the control group. The GO terms enrichment analysis of the differentially expressed miRNAs indicated that oxidation reduction was the most enriched process. Low expression of miR-30 family members in human HNSCC cell lines and tissues was validated by qPCR. The results revealed that the expression of miR-30b-5p and miR-30e-5p was significantly decreased in the TCGA HNSCC dataset and validation datasets, and this decrease in expression further distinguishes HNSCC associated with tobacco use from other subtypes of HNSCC. CCK8, colony formation, transwell migration and HNSCC xenograft tumor assays indicated that miR-30b-5p or miR-30e-5p inhibited proliferation, migration and invasion in vitro, and miR-30b-5p suppressed tumor growth in vivo. Moreover, we uncovered that KRAS might be the potential target gene of miR-30e-5p or miR-30b-5p. Thus, our data clearly showed that decreased expression of miR-30e-5p or miR-30b-5p may play a crucial role in cancer development, especially that of tobacco-induced HNSCC, and may be a novel candidate biomarker and target for this HNSCC subtype.

microRNA Identification on Head and Neck Squamous Cell Carcinoma Cancer Gene based on Male Gender

2021 ◽  
Vol 16 (8) ◽  
pp. 50-54
Author(s):  
Maulani Wijaya Renadya ◽  
David Agustriawan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Expression Data ◽  
Cancer Genes ◽  
Altered Expression ◽  
Data Portal ◽  
Nose And Throat

Head and neck squamous cell carcinoma (HNSC) is a cancer that develops within the mucous membranes around mouth, nose and throat area. The risk factors for developing this type of cancer are tobacco use and serious alcohol consumption. Altered expression of microRNAs (miRNAs) might become novel molecular biomarkers to identify cancer, metastatic site and even the cancer stage. This study aims to identify how miRNA would contribute to the development on HNSC in a specific population of male since males have a higher tendency to get this cancer than females because of the drinking and smoking habits. This study relies on the expression data obtained from NIH GDC Data Portal using TCGA-Assembler. The data were filtered and differentially expressed genes (DEGs) and miRNAs (DEMs) were determined. Afterwards, correlation test was applied to expression data by using MATLAB. Statistical analyses of expression data identified potential miRNAs targeting cancer genes as well as hsa-mir-31, hsa-mir-193b and hsa-mir-205. Further validation analysis can be conducted using RNAhybrid and examination with alternative miRNA databases.

Altered expression of SIRT gene family in head and neck squamous cell carcinoma

Tumor Biology ◽  
2013 ◽  
Vol 34 (3) ◽  
pp. 1847-1854 ◽  
Author(s):  
Chi-Chih Lai ◽  
Pai-Mei Lin ◽  
Sheng-Fung Lin ◽  
Cheng-Hsien Hsu ◽  
Hsin-Ching Lin ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Gene Family ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Altered Expression
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