scholarly journals Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Ireneusz Sowa ◽  
Sylwia Zielińska ◽  
Jan Sawicki ◽  
Anna Bogucka-Kocka ◽  
Michał Staniak ◽  
...  
Keyword(s):  
Antimicrobial Activities ◽  
Systematic Evaluation ◽  
Concentration Addition ◽  
Core Shell ◽  
Isoquinoline Alkaloids ◽  
Chelidonium Majus ◽  
Chromatographic System ◽  
Peak Asymmetry ◽  
Chromatographic Parameters ◽  
New Generation

Chelidonium majusL. is a rich source of isoquinoline alkaloids with confirmed anti-inflammatory, choleretic, spasmolytic, antitumor, and antimicrobial activities. However, their chromatographic analysis is difficult because they may exist both in charged and uncharged forms and may result in the irregular peak shape and the decrease in chromatographic system efficacy. In the present work, the separation of mainC. majusalkaloids was optimized using a new-generation XB-C18 endcapped core-shell column dedicated for analysis of alkaline compounds. The influence of organic modifier concentration, addition of salts, and pH of eluents on chromatographic parameters such as retention, resolution, chromatographic plate numbers, and peak asymmetry was investigated. The results were applied to elaborate the optimal chromatographic system for simultaneous quantification of seven alkaloids from the root, herb, and fruit ofC. majus.

scholarly journals Applicability of a Monolithic Column for Separation of Isoquinoline Alkalodis from Chelidonium majus Extract

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3612 ◽  
Author(s):  
Staniak ◽  
Wójciak-Kosior ◽  
Sowa ◽  
Strzemski ◽  
Sawicki ◽  
...  
Keyword(s):  
Monolithic Column ◽  
Gradient Elution ◽  
Root Extract ◽  
Organic Modifier ◽  
Isoquinoline Alkaloids ◽  
Chelidonium Majus ◽  
Peak Asymmetry ◽  
Application Potential ◽  
Chromatographic Plate ◽  
Chromatographic Parameters

Isoquinoline alkaloids are the main group of secondary metabolites present in Chelidonium majus extracts, and they are still the object of interest of many researchers. Therefore, the development of methods for the investigation and separation of the alkaloids is still an important task. In this work, the application potential of a silica-based monolithic column for the separation of alkaloids was assessed. The influence of the organic modifier, temperature, salt concentration, and pH of the eluent on basic chromatographic parameters such as retention, resolution between neighboring peaks, chromatographic plate numbers, and peak asymmetry were investigated. Based on the obtained results, a gradient elution program was developed and used to separate and quantitatively determine the main alkaloids in a Chelidonium majus root extract.

scholarly journals Synthesis and Characterization of a Fe3O4@PNIPAM-Chitosan Nanocomposite and Its Potential Application in Vincristine Delivery

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1704
Author(s):  
Cynthia N. Hernández-Téllez ◽  
Ana G. Luque-Alcaraz ◽  
Maribel Plascencia-Jatomea ◽  
Hiram J. Higuera-Valenzuela ◽  
Mabeth Burgos-Hernández ◽  
...  
Keyword(s):  
Fe3o4 Nanoparticles ◽  
Release Kinetics ◽  
Magnetic Core ◽  
Systematic Evaluation ◽  
Solution Temperature ◽  
Core Shell ◽  
Particle Size And Morphology ◽  
Size And Morphology ◽  
Thermomagnetic Properties ◽  
Release Model

In this research, we conducted a systematic evaluation of the synthesis parameters of a multi-responsive core-shell nanocomposite (Fe3O4 nanoparticles coated by poly(N-isopropylacrylamide) (PNIPAM) in the presence of chitosan (CS) (Fe3O4@PNIPAM-CS). Scanning electron microscopy (SEM) was used to follow the size and morphology of the nanocomposite. The functionalization and the coating of Fe3O4 nanoparticles (Nps) were evaluated by the ζ-potential evolution and Fourier Transform infrared spectroscopy (FTIR). The nanocomposite exhibited a collapsed structure when the temperature was driven above the lower critical solution temperature (LCST), determined by dynamic light scattering (DLS). The LCST was successfully shifted from 33 to 39 °C, which opens the possibility of using it in physiological systems. A magnetometry test was performed to confirm the superparamagnetic behavior at room temperature. The obtained systems allow the possibility to control specific properties, such as particle size and morphology. Finally, we performed vincristine sulfate loading and release tests. Mathematical analysis reveals a two-stage structural-relaxation release model beyond the LCST. In contrast, a temperature of 25 °C promotes the diffusional release model. As a result, a more in-depth comprehension of the release kinetics was achieved. The synthesis and study of a magnetic core-shell nanoplatform offer a smart material as an alternative targeted release therapy due to its thermomagnetic properties.

Co-Extrusion Technology for Functioned Nature Fiber Reinforced Polymer Composites

2013 ◽  
Vol 773 ◽  
pp. 497-501
Author(s):  
Run Zhou Huang ◽  
Cheng Jun Zhou ◽  
Yang Zhang ◽  
Qing Lin Wu
Keyword(s):  
Polymer Composites ◽  
Shell Structure ◽  
Critical Role ◽  
Core Shell ◽  
Green Composite ◽  
Reinforced Polymer ◽  
Fiber Reinforced Polymer Composites ◽  
Core Shell Structure ◽  
Extrusion Technology ◽  
New Generation

As a new-generation green composite, core-shell structure nature fibers/ polymer composites (NFPC) has been recently developed and used to enhance performance characteristics of composites. The shell layer, made of thermopolymers unfilled or filled with minerals or nature fibers and other additives, plays a critical role in enhancing overall composite properties. The co-extrusion technology for different structural and material combinations are, however, needed to achieve desired processing. In this paper, co-extrusion technology and core-shell structure NFPC have been discussed by systematically studying the effect of structure on properties of NFPC. The result of this paper can help provide a fundamental base for developing new functional applications of core-shell structure NFPCs.

scholarly journals Acetylcholinesterase and Butyrylcholinesterase Inhibitory Compounds from Chelidonium Majus (Papaveraceae)

2010 ◽  
Vol 5 (11) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Lucie Cahlíková ◽  
Lubomír Opletal ◽  
Milan Kurfürst ◽  
Kateřina Macáková ◽  
Andrea Kulhánková ◽  
...  
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Inhibitory Activity ◽  
Dependent Manner ◽  
Isoquinoline Alkaloids ◽  
Chelidonium Majus ◽  
Aerial Parts ◽  
Naturally Occurring ◽  
Inhibitory Compounds ◽  
Dose Dependent

The roots and aerial parts of Chelidonium majus L. were extracted with EtOH and fractionated using CHCl3 and EtOH. Repeated column chromatography, preparative TLC and crystallization led to the isolation of five isoquinoline alkaloids, stylopine (3), chelidonine (4), homochelidonine (5), protopine (6), and allocryptopine (7), along with two isolation artifacts 6-ethoxydihydrosanguinarine (1) and 6-ethoxydihydrochelerythrine (2). All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. The isolation artifacts exhibited the highest activity against HuAChE and HuBuChE with IC50 values of 0.83 ± 0.04 μM and 4.20 ± 0.19 μM for 6-ethoxydihydrochelerythrine and 3.25 ± 0.24 μM and 4.51 ± 0.31 μM for 6-ethoxydihydrosanguinarine. The most active of the naturally-occurring alkaloids was chelidonine, which inhibited both HuAChE and HuBuChE in a dose-dependent manner with IC50 values of 26.8 ± 1.2 μM and 31.9 ± 1.4 μM, respectively.

Synthesis, radical scavenging, and antimicrobial activities of core–shell Au/Ni microtubes

2020 ◽  
Vol 74 (7) ◽  
pp. 2189-2199
Author(s):  
Anastassiya A. Mashentseva ◽  
Milana A. Ibragimova ◽  
Saule B. Akhmetova ◽  
Artem L. Kozlovskiy ◽  
Maxim V. Zdorovets ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Core Shell

scholarly journals Systematic Evaluation of Light-Activatable Biohybrids for Anti-Glioma Photodynamic Therapy

2019 ◽  
Vol 8 (9) ◽  
pp. 1269 ◽  
Author(s):  
Inglut ◽  
Baglo ◽  
Liang ◽  
Cheema ◽  
Stabile ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Systematic Evaluation ◽  
Therapeutic Effects ◽  
End Effects ◽  
Fundamental Understanding ◽  
Effective Use ◽  
Oxygen Yield ◽  
New Generation

Photosensitizing biomolecules (PSBM) represent a new generation of light-absorbing compounds with improved optical and physicochemical properties for biomedical applications. Despite numerous advances in lipid-, polymer-, and protein-based PSBMs, their effective use requires a fundamental understanding of how macromolecular structure influences the physicochemical and biological properties of the photosensitizer. Here, we prepared and characterized three well-defined PSBMs based on a clinically used photosensitizer, benzoporphyrin derivative (BPD). The PSBMs include 16:0 lysophosphocholine-BPD (16:0 Lyso PC-BPD), distearoyl-phosphoethanolamine-polyethylene-glycol-BPD (DSPE-PEG-BPD), and anti-EGFR cetuximab-BPD (Cet-BPD). In two glioma cell lines, DSPE-PEG-BPD exhibited the highest singlet oxygen yield but was the least phototoxic due to low cellular uptake. The 16:0 Lyso PC-BPD was most efficient in promoting cellular uptake but redirected BPD’s subcellular localization from mitochondria to lysosomes. At 24 h after incubation, proteolyzed Cet-BPD was localized to mitochondria and effectively disrupted the mitochondrial membrane potential upon light activation. Our results revealed the variable trafficking and end effects of PSBMs, providing valuable insights into methods of PSBM evaluation, as well as strategies to select PSBMs based on subcellular targets and cytotoxic mechanisms. We demonstrated that biologically informed combinations of PSBMs to target lysosomes and mitochondria, concurrently, may lead to enhanced therapeutic effects against gliomas.

scholarly journals Screening Papaveraceae as Novel Antibiofilm Natural-Based Agents

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4778
Author(s):  
Sylwia Zielińska ◽  
Magdalena Dziągwa-Becker ◽  
Adam Junka ◽  
Ewelina Piątczak ◽  
Anna Jezierska-Domaradzka ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Properties ◽  
Isoquinoline Alkaloids ◽  
Human Pathogens ◽  
Chelidonium Majus ◽  
Bioactive Substances ◽  
Aerial Parts ◽  
Aconitic Acid ◽  
The Impact

The antimicrobial properties of herbs from Papaveraceae have been used in medicine for centuries. Nevertheless, mutual relationships between the individual bioactive substances contained in these plants remain poorly elucidated. In this work, phytochemical composition of extracts from the aerial and underground parts of five Papaveraceae species (Chelidonium majus L., Corydalis cava (L.) Schweigg. and Körte, C. cheilanthifolia Hemsl., C. pumila (Host) Rchb., and Fumaria vaillantii Loisel.) were examined using LC-ESI-MS/MS with a triple quadrupole analyzer. Large differences in the quality and quantity of all analyzed compounds were observed between species of different genera and also within one genus. Two groups of metabolites predominated in the phytochemical profiles. These were isoquinoline alkaloids and, in smaller amounts, non-phenolic carboxylic acids and phenolic compounds. In aerial and underground parts, 22 and 20 compounds were detected, respectively. These included: seven isoquinoline alkaloids: protopine, allocryptopine, coptisine, berberine, chelidonine, sanguinarine, and chelerythrine; five of their derivatives as well as non-alkaloids: malic acid, trans-aconitic acid, quinic acid, salicylic acid, trans-caffeic acid, p-coumaric acid, chlorogenic acid, quercetin, and kaempferol; and vanillin. The aerial parts were much richer in phenolic compounds regardless of the plant species. Characterized extracts were studied for their antimicrobial potential against planktonic and biofilm-producing cells of S. aureus, P. aeruginosa, and C. albicans. The impact of the extracts on cellular metabolic activity and biofilm biomass production was evaluated. Moreover, the antimicrobial activity of the extracts introduced to the polymeric carrier made of bacterial cellulose was assessed. Extracts of C. cheilanthifolia were found to be the most effective against all tested human pathogens. Multiple regression tests indicated a high antimicrobial impact of quercetin in extracts of aerial parts against planktonic cells of S. aureus, P. aeruginosa, and C. albicans, and no direct correlation between the composition of other bioactive substances and the results of antimicrobial activity were found. Conclusively, further investigations are required to identify the relations between recognized and unrecognized compounds within extracts and their biological properties.

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