scholarly journals Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Raha Orfali ◽  
Nasir Ali Siddiqui ◽  
Perwez Alam ◽  
Tawfeq Abdullah Alhowiriny ◽  
Areej Mohammad Al-Taweel ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ursolic Acid ◽  
Folk Medicine ◽  
Biological Evaluation ◽  
Bioactive Metabolites ◽  
Cytotoxic Activities ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Hptlc Method ◽  
Acid Compound

Nepeta deflersiana (Lamiaceae) is a well-known medicinal plant that grows in Saudi Arabia. This plant is used in Saudi and Yemeni folk medicine as an anti-inflammatory, carminative, and antirheumatic agent. In order to prove its use in folk medicine, four different extracts from the aerial parts of the plant: petroleum ether, chloroform, ethyl acetate, and n-butanol extracts were subjected to biological assays to screen PPARα and PPARϒ agnostic, antioxidant, anti-inflammatory, and cytotoxic activities. Ethyl acetate and n-butanol extracts of N. deflersiana NDEE and NDBE, respectively, showed a decrease in oxidative stress and inhibition of both NF-kB and iNOS activities with no cytotoxic effects on four human cancer cell lines. Both active extracts were standardized using two bioactive metabolites which were isolated from the aerial parts of the same plant [8-epi-7-deoxyloganic acid (compound 1) and Ursolic acid (compound 2)] by developing a validated HPTLC method. It was found to provide a sharp and compact band of compound 1 at Rf = 0.07 and Rf = 0.57 for compound 2, using chloroform, methanol, and formic acid (8.9:0.8:0.3, v/v/v) as mobile phase at 550 nm. Compounds 1 and 2 were found in NDEE by 9.59 %, w/w, and 84.63 %, w/w, respectively, and by 11.97 %, w/w, and 21.26 %, w/w, respectively, in NDBE.

scholarly journals Phytochemical Screening, Biological Studiesand GC-MS Analysis of Extract of Biden pilosa L

2019 ◽  
Vol 5 (1) ◽  
pp. 79-93
Author(s):  
Dharma Prasad Khanal ◽  
Rupa Rana ◽  
Bechan Raut ◽  
Rabindra Prasad Dhakal
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Inflammatory Activity ◽  
Alcoholic Extract ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Bidens Pilosa ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Anti Inflammatory

Objective: The aim of the research work was to carry out the extraction of areal parts of Biden pilosa L by hydroalcholic and Hexane, ethyl acetate and acetone mixture followed by qualitative phytochemical analysis, acute oral toxicity test, anti-inflammatory test and GC-MS analysis of the extracts. Method: The hydro-alcoholic and HEA(n-hexane, ethyl acetate and acetone) extraction was done from aerial parts using ethanol and water in the ratio 70:30 and n-hexane, ethyl acetate and acetone in the ratio of 1:1:1 (HEA extract)  respectively. Acute oral toxicity testwas performed OECD guidelines. The single spot in TLC was obtained using n-hexane as solvent for HEA fraction and finally phytocomponents were identified by GC-MS present in that spot.In vitro anti-inflammatory activity was performed by human RBC membrane stabilization method. Result: The phytochemical test results obtained indicate that hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. Both extracts showed significant in vitro anti-inflammatory activity in a concentration dependent manner. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds from partially separated extract from TLC plates. Conclusion: Hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds.

scholarly journals Three New Cytotoxic Withanolides from the Chinese Folk Medicine Physalis angulata

2015 ◽  
Vol 10 (12) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Cai-Yun Gao ◽  
Ting Ma ◽  
Jun Luo ◽  
Ling-Yi Kong
Keyword(s):  
Cell Line ◽  
Folk Medicine ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Spectroscopic Techniques ◽  
Aerial Parts ◽  
Physalis Angulata ◽  
The Absolute ◽  
New Compounds

Physagulides M-O, three new withanolides (1–3), were isolated from the aerial parts of Physalis angulata L. Their structures were elucidated through extensive spectroscopic techniques, including 1D and 2D NMR, and HRESIMS. The absolute configurations (22- R) of these new compounds were determined by CD analysis. Compounds 1 and 3 showed significant selective cytotoxic activities on the MG-63 cell line, with IC50 values of 4.28 and 5.44 μM, respectively.

Formulation and Evaluation of Anti-inflammatory Herbal gel using Sarcosteema acidum

2020 ◽  
Vol 1 (28) ◽  
pp. 62-69
Keyword(s):  
Medicinal Plants ◽  
Blood Cells ◽  
Defense Mechanism ◽  
Folk Medicine ◽  
Biological Evaluation ◽  
Safety And Efficacy ◽  
Complex Events ◽  
Anti Inflammatory ◽  
Living Tissues ◽  
Local Accumulation

Abstract-Inflammation is a pathophysiological response of living tissues to injuries that leads to the local accumulation of plasmatic fluid and blood cells. Although it is a defense mechanism, the complex events and mediators involved the inflammatory reaction can induce, maintain or aggravate many diseases. Therefore, the uses of anti-inflammatory agents are helpful in the therapeutic treatment of these pathologies. In this context, medicinal plants are widely used in folk medicine of many countries to treat differentinflammatoryconditionsand,inparticular, skin inflammations. Present work was designed to formulate herbal gel using extract of Sarcosteema acidumstem which provide better efficacy and to ensure biological evaluation of prepared herbal gel for safety and efficacy.

scholarly journals Anticancer activity and concurrent analysis of ursolic acid, β-sitosterol and lupeol in three different Hibiscus species (aerial parts) by validated HPTLC method

2018 ◽  
Vol 26 (7) ◽  
pp. 1060-1067 ◽  
Author(s):  
Perwez Alam ◽  
Hanan M. Al-Yousef ◽  
Nasir A. Siddiqui ◽  
Tawfeq A. Alhowiriny ◽  
Saleh I. Alqasoumi ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Ursolic Acid ◽  
Aerial Parts ◽  
Hptlc Method ◽  
Concurrent Analysis

Anti-inflammatory effect of the methanol, ethyl acetate and chloroform extracts of Tragopogon porrifolius aerial parts

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
EH Hariri ◽  
M El Asmar ◽  
SA Demirdjian ◽  
CF Daher ◽  
MA Mroueh
Keyword(s):  
Ethyl Acetate ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Metabolomic Profiling, Antioxidant and Anti-inflammatory Activities of Hypericum Species Growing in South Korea

2017 ◽  
Vol 12 (7) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Heung Joo Yuk ◽  
Kyeong Yeol Oh ◽  
Doo-Young Kim ◽  
Hyuk-Hwan Song ◽  
Jun Young Kim ◽  
...  
Keyword(s):  
Biological Activities ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Principal Component ◽  
Ultra Performance Liquid Chromatography ◽  
Raw 264.7 Cells ◽  
Bioactive Metabolites ◽  
Flight Mass Spectrometry ◽  
Anti Inflammatory ◽  
Hypericum Species

Although Hypericum species are best known as plants that produce hypericin and are used in folk medicine, their other chemical constituents are poorly understood. Polyphenolic secondary metabolites from whole plants of representative Korean Hypericum species ( H. laxum Koidz., H. erectum Thunb., and H. ascyron L.) were analyzed using a ultra performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS)-based approach combined with unsupervised principal component analysis (PCA) multivariate analysis. On the loading scatter plot, significant changes in metabolites were found between species, and three flavonol glycosides (8: quercetin-3- O-galactoside, 12: kaempferol-3- O-glucoside, and 13: quercetin-3- O-rhamnoside) were evaluated as key markers among 17 isolated metabolites. The extracts of H. laxum Koidz. exhibited significant quenching effects on DPPH and ABTS radicals, with IC50 values of 10–20 μg/mL, and were slightly higher in total phenol (TP) and total flavonoid (TF) contents than other species. Additionally, anti-inflammatory activity was observed by reduced nitric oxide (NO) and interleukin-6 (IL-6) production from lipopolysaccharide (LPS)-stimulated macrophages (RAW 264.7 cells). This is the first study to report the presence of bioactive metabolites and their correlating biological activities in H. laxum Koidz.

scholarly journals Ducrosia spp., Rare Plants with Promising Phytochemical and Pharmacological Characteristics: An Updated Review

2020 ◽  
Vol 13 (8) ◽  
pp. 175
Author(s):  
Javad Mottaghipisheh ◽  
Anahita Boveiri Dehsheikh ◽  
Mohammad Mahmoodi Sourestani ◽  
Tivadar Kiss ◽  
Judit Hohmann ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Common Species ◽  
Scientific Data ◽  
Rare Plants ◽  
Cytotoxic Activities ◽  
Analgesic Effects ◽  
Aerial Parts ◽  
Ethnobotanical Uses

The rare genus Ducrosia (Apiaceae family) consists of six species, which are mainly native to Asia, specifically to Iran and Iraq. The aerial parts of D. anethifolia, as the most common species, have been traditionally consumed to relieve headache, backache and colic pain, and have also been used as an anxiolytic, an antidepressant, and for treating insomnia. The antispasmodic and carminative effects of D. assadii, and the analgesic activity of D. flabellifolia, along with the insecticidal activities and use as a remedy of skin infections of D. ismaelis, have been previously documented. Among the 49 non-volatile secondary metabolites identified from D. anethifolia and D. ismaelis, 17 linear furanocoumarins and 8 flavonoids have been characterized. The essential oil compositions of four species, including D. anethifolia, D. assadii, D. flabellifolia and D. ismaelis, have been analyzed, whereby aldehyde hydrocarbons, including decanal (10.1–74.0%) and dodecanal (7.2–33.41%), and α-pinene (4.0–70.3%), were identified as the main aroma constituents. From the species of the genus, the bioactivities of D. anethifolia, as well as D. ismaelis, D. assadii and D. flabellifolia, have been previously investigated. Except one clinical trial, all the pharmacological data are derived from preclinical tests, predominantly focusing on antimicrobial, antioxidant, antiproliferative and cytotoxic activities in vitro, and neuroprotective, antidiabetic and analgesic effects in vivo. Considering the vast ethnobotanical uses of the plants in Iranian folk medicine, the phytochemical and pharmacological analysis of un-investigated species might be promising. Furthermore, due to extensive consumption of the Ducrosia genus, more scientific data are needed to support the safety and efficacy of these plants.

In vivo Analgesic and Anti-Inflammatory Activities of Ursolic Acid and Oleanoic Acid from Miconia albicans (Melastomataceae)

2006 ◽  
Vol 61 (7-8) ◽  
pp. 477-482 ◽  
Author(s):  
Maria Anita L. Vasconcelos ◽  
Vanessa A. Royo ◽  
Daniele S. Ferreira ◽  
Antonio E. Miller Crotti ◽  
Márcio L. Andrade e Silva ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Crude Extract ◽  
Methylene Chloride ◽  
Second Phase ◽  
Dependent Manner ◽  
In Vivo Models ◽  
Isomeric Mixture ◽  
Aerial Parts ◽  
Anti Inflammatory

The aim of this work was to use in vivo models to evaluate the analgesic and anti-inflammatory activities of ursolic acid (UA) and oleanoic acid (OA), the major compounds isolated as an isomeric mixture from the crude methylene chloride extract of Miconia albicans aerial parts, in an attempt to clarify if these compounds are responsible for the analgesic properties displayed by this plant. Ursolic acid inhibited abdominal constriction in a dose-dependent manner, and the result obtained at a content of 40 mg kg-1 was similar to that produced by administration of acetylsalicylic acid at a content of 100 mg kg-1. Both acids reduced the number of paw licks in the second phase of the formalin test, and both of them displayed a significant anti-inflammatory effect at a content of 40 mg kg-1. It is noteworthy that the administration of the isolated mixture, containing 65% ursolic acid/35% oleanolic acid, did not display significant analgesic and anti-inflammatory activities. On the basis of the obtained results, considering that the mixture of UA and OA was poorly active, it is suggested that other compounds, rather than UA and OA, should be responsible for the evaluated activities in the crude extract, since the crude extract samples displayed good activities.

scholarly journals Mushrooms: A Potential Natural Source of Anti-Inflammatory Compounds for Medical Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Elsayed A. Elsayed ◽  
Hesham El Enshasy ◽  
Mohammad A. M. Wadaan ◽  
Ramlan Aziz
Keyword(s):  
Chemical Structure ◽  
Nutritional Value ◽  
Folk Medicine ◽  
Mechanisms Of Action ◽  
Bioactive Metabolites ◽  
Low Molecular Weight ◽  
Potential Source ◽  
Different Types ◽  
Anti Inflammatory ◽  
Different Parts

For centuries, macrofungi have been used as food and medicine in different parts of the world. This is mainly attributed to their nutritional value as a potential source of carbohydrates, proteins, amino acids, and minerals. In addition, they also include many bioactive metabolites which make mushrooms and truffles common components in folk medicine, especially in Africa, the Middle East, China, and Japan. The reported medicinal effects of mushrooms include anti-inflammatory effects, with anti-inflammatory compounds of mushrooms comprising a highly diversified group in terms of their chemical structure. They include polysaccharides, terpenoids, phenolic compounds, and many other low molecular weight molecules. The aims of this review are to report the different types of bioactive metabolites and their relevant producers, as well as the different mechanisms of action of mushroom compounds as potent anti-inflammatory agents.

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