scholarly journals Metabolomic Profiling of Human Spermatozoa in Idiopathic Asthenozoospermia Patients Using Gas Chromatography-Mass Spectrometry

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Kai Zhao ◽  
Jianzhong Zhang ◽  
Zhen Xu ◽  
Yue Xu ◽  
Aiming Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Krebs Cycle ◽  
Human Sperm ◽  
Gas Chromatography Mass Spectrometry ◽  
Sperm Cells ◽  
Spectrometric Data ◽  
Metabolomic Profiling ◽  
Chromatography Mass Spectrometry ◽  
First Time

The purpose of this study was to describe the first metabolic profile of human sperm cells through the application of an untargeted platform based on gas chromatography-mass spectrometry (GC-MS). Sperm cell samples from patients diagnosed with idiopathic asthenozoospermia (n=30) and healthy subjects (n=30) were analyzed using a nontargeted metabolomics method based on GC-MS spectroscopy. The mass spectrometric data were collected using multivariate and univariate analyses to identify metabolites related to idiopathic asthenozoospermia. By using metabolomic strategies, we identified 33 metabolites, 27 of which were decreased in the idiopathic asthenozoospermia group compared with the normozoospermic group and six were increased in idiopathic asthenozoospermia. With respect to human sperm cells, some of these metabolites are reported here for the first time. Pathways for nucleoside, amino acid and energy metabolism, and the Krebs cycle were disturbed and were associated with idiopathic asthenozoospermia. The metabolic profiling provides an important first step in studying the pathophysiological mechanisms involved in IAS, and the identified metabolites may become potential biomarkers for its diagnosis and treatment.

Identification by Gas Chromatography-Mass Spectrometry of 150 Compounds in Propolis

1991 ◽  
Vol 46 (1-2) ◽  
pp. 111-121 ◽  
Author(s):  
W. Greenaway ◽  
J. May ◽  
T. Scaysbrook ◽  
F. R. Whatley
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Alcoholic Extract ◽  
Headspace Volatiles ◽  
Chromatography Mass Spectrometry ◽  
First Time

Abstract Propolis was analyzed by gas chromatography-mass spectrometry for both its headspace volatiles and for the less volatile components of its alcoholic extract (propolis balsam). 181 peaks were located of which 171 representing 150 compounds were identified, including 28 identified in propolis for the first time. The majority of compounds were typical of poplar bud exudate.

Determination of Polychlorodibenzo-p-dioxins and Related Compounds in Commercial Chlorophenols

1972 ◽  
Vol 55 (1) ◽  
pp. 85-92 ◽  
Author(s):  
David Firestone ◽  
John Ress ◽  
N L Brown ◽  
R P Barron ◽  
J N Damico
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Electron Capture ◽  
Gas Chromatography Mass Spectrometry ◽  
Alumina Column ◽  
Spectrometric Data ◽  
Chromatography Mass Spectrometry ◽  
Related Compounds ◽  
Chromatographic Mass

Abstract Twenty-one commercial chlorophenols were examined for the presence of polychlorodibenzo- p-dioxins (chlorodioxins) and related compounds. The chlorophenols were dissolved in aqueous alkali, extracted with petroleum ether, and fractionated on an alumina column. Alumina fractions were examined by electron capture gas chromatography and combined gas chromatography-mass spectrometry. Chlorodioxin content was estimated by electron capture gas chromatography. The presence of chlorodioxins, polychlorodibenzofurans (chlorofurans), and polychlorodiphenyl ethers (chloroethers) was confirmed by combined gas chromatography-mass spectrometry. The 2,3,-7,8-tetrachlorodioxin was found in 3 of 6 samples of 2,4,5-trichlorophenol but was not detected in any of the 11 samples of tetra- and pentachlorophenol that were examined. Hexachlorodioxin was present at levels ranging from 0.17 to 39 ppm in all 8 pentachlorophenols examined. Hexa-, hepta-, and octachlorodioxins as well as a wide variety of chlorofurans and chloroethers of varying chlorine content were present in most of the tetra- and pentachlorophenols. In addition, the gas chromatographic- mass spectrometric data suggested that some of the chlorophenols contained methoxy- and dimethoxypolychlorofurans and methoxypolychloroethers as well as polychlorohy droxybiphenyl.

Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry

2004 ◽  
Vol 385 (9) ◽  
pp. 853-861 ◽  
Author(s):  
Sergey Strelkov ◽  
Mirko von Elstermann ◽  
Dietmar Schomburg
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Corynebacterium Glutamicum ◽  
Growth Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Fast Method ◽  
Chromatography Mass Spectrometry ◽  
High Matrix ◽  
First Time ◽  
Derivatives Of

AbstractAn analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation ofCorynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation ofC. glutamicumto different growth conditions is demonstrated.

scholarly journals Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry

2012 ◽  
Vol 76 (8) ◽  
pp. 1864-1873 ◽  
Author(s):  
Minoru Hasokawa ◽  
Masakazu Shinohara ◽  
Hiroshi Tsugawa ◽  
Takeshi Bamba ◽  
Eiichiro Fukusaki ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Stent Restenosis ◽  
Metabolomic Profiling ◽  
Chromatography Mass Spectrometry

scholarly journals A validated method for the analysis and profiling of ‘nyaope’ using gas chromatography – mass spectrometry

2021 ◽  
Vol 117 (11/12) ◽  
Author(s):  
Pabalala M. Mthembi ◽  
Ellen M. Mwenesongole ◽  
Michael D. Cole
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Principal Component ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Drug Cocktail ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
First Time ◽  
Problem Drug

Nyaope, a Tswana word for a mixture or ‘mish-mash’, describes a drug cocktail consisting of heroin, cannabis, and on occasion other controlled substances and warfarin. It is highly addictive with extremely unpleasant side effects caused by withdrawal from the drug. It is a problem drug especially in townships in South Africa. However, its prevalence in neighbouring southern African states and further afield is not yet known. There is currently no validated method for the analysis and comparison of nyaope. We describe a validated method for the gas chromatography – mass spectrometry analysis of nyaope so that within-batch and between-batch comparisons of nyaope can successfully be made for the first time. The validated method managed an accuracy within the range 80–120%, the precision was less than 20% for all analytes and managed linearity with R2≥0.99. The detection limits for diamorphine, efavirenz, nevirapine and Δ9-tetrahydrocannabinol were 14.2, 18.6, 18.7 and 9.94 pg on column, respectively, and the limits of quantitation were 43.1, 56.3, 56.6 and 30.1 pg on column, respectively. The simulated and casework samples were successfully discriminated into original batches using the identified nyaope components, the unsupervised chemometric methods principal component analysis and hierarchical clustering, as well as chromatographic profiles.

scholarly journals CHEMICAL COMPOSITION OF THE ESSENTIAL OIL FROM LEAVES OF CALLICARPA ARBOREA ROXB. GROWING IN VIETNAM

2021 ◽  
Vol 58 (6A) ◽  
pp. 280
Author(s):  
Hung Huy Nguyen ◽  
Dai Ngoc Do ◽  
Prabodh Satyal ◽  
Chung Thanh Nguyen ◽  
Nguyen Van Bui ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Chromatography Mass Spectrometry ◽  
First Time

The essential oil from the leaves of Callicarpa arborea Roxb. growing wild in Vietnam, was obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. The major constituents of leaves of C. arborea were β-selinene (23.9%), ar-turmerone (17.5%) and α-copaene (8.9%), with lesser concentrations of caryophyllene oxide (4.9%) and ar-turmerol (3.9%). The chemical composition of this essential oil sample is being reported for the first time.

scholarly journals Quantification of VOC Emissions from Carbonized Refuse-Derived Fuel Using Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3208 ◽  
Author(s):  
Andrzej Białowiec ◽  
Monika Micuda ◽  
Antoni Szumny ◽  
Jacek Łyczko ◽  
Jacek Koziel
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Municipal Waste ◽  
Gas Chromatography Mass Spectrometry ◽  
Voc Emissions ◽  
Chromatography Mass Spectrometry ◽  
Refuse Derived Fuel ◽  
First Time

In this work, for the first time, the volatile organic compound (VOC) emissions from carbonized refuse-derived fuel (CRDF) were quantified on a laboratory scale. The analyzed CRDF was generated from the torrefaction of municipal waste. Headspace solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) was used to identify 84 VOCs, including many that are toxic, e.g., derivatives of benzene or toluene. The highest emissions were measured for nonanal, octanal, and heptanal. The top 10 most emitted VOCs contributed to almost 65% of the total emissions. The VOC mixture emitted from torrefied CRDF differed from that emitted by other types of pyrolyzed biochars, produced from different types of feedstock, and under different pyrolysis conditions. SPME was a useful technology for surveying VOC emissions. Results provide an initial database of the types and relative quantities of VOCs emitted from CRDF. This data is needed for further development of CRDF technology and comprehensive assessment of environmental impact and practical storage, transport, and potential adoption of CRDF as means of energy and resource recovery from municipal waste.

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