scholarly journals Antidiarrheal and Antioxidant Activities of Methanol Extract of Bryophyllum pinnatum Leaf Harvested from South-Eastern Nigeria in Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Samuel O. Onoja ◽  
Ginika Q. Ihejirika ◽  
Oluchi N. Nwankudu ◽  
Yusuf N. Omeh ◽  
Maxwell I. Ezeja
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Intestinal Transit ◽  
Distilled Water ◽  
Small Intestinal Transit ◽  
Antioxidant Power ◽  
South Eastern ◽  
Small Intestinal ◽  
Bryophyllum Pinnatum

Bryophyllum pinnatum belongs to the family Crassulaceae and it is commonly used in the ethnomedical practices. This study investigated the antidiarrheal and antioxidant properties of methanol extract of Bryophyllum pinnatum leaf harvested from South-Eastern Nigeria in mice. Cold maceration method in 80% methanol was adopted in the extract preparation. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays were used to evaluate the antioxidant property while castor oil-induced diarrhea, small intestinal transit, and enteropooling models were used for the antidiarrheal investigation. The effects of the extract (50, 100, and 200 mg/kg) were compared to distilled water (10 ml/kg) and loperamide (5 mg/kg). The extract produced concentration dependent increase in antioxidant effect in both DPPH and FRAP assay. The extract caused a significant (p<0.05) reduction in mean stool output, percentage of wet stools, small intestinal transit, and intestinal fluid accumulation in the treated mice when compared to the distilled water treated mice. The study validates the use of Bryophyllum pinnatum in the ethnomedical management of diarrhea.

Antioxidant, hypoglycemic and anti-hypertensive properties of extracts derived from peel, fruit and kernel of Salak

2020 ◽  
Vol 122 (10) ◽  
pp. 3029-3038
Author(s):  
Seok Shin Tan ◽  
Seok Tyug Tan ◽  
Chin Xuan Tan
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Alpha Amylase ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Distilled Water ◽  
Antioxidant Power ◽  
Content Type ◽  
Fruit Extracts ◽  
Significant Difference

PurposeSalak (Salacca zalacca) is an underutilised fruit. The bioactivities of this fruit have rarely been studied scientifically. Thus, the present study aimed to determine the antioxidant activity of extracts derived from the peel, fruit and kernel of the Salak fruit, as well as the hypoglycemic and anti-hypertensive properties of Salak peel extracts.Design/methodology/approachThe peel, fruit and kernel of the Salak were extracted using distilled water, methanol and ethanol. Antioxidant activities, angiotensin-converting enzyme (ACE) and alpha-amylase inhibition properties of the extracts were estimated via in vitro standard methods. Besides, the total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were also determined in the present study. The antioxidant activities of different parts of Salak extracts were determined by ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) methods. Percent of radical scavenging properties were calculated via DPPH assay. The hypoglycemic and anti-hypertensive properties of Salak peel were evaluated using alpha-amylase inhibition and ACE assays, respectively.FindingsFruit extracts of Salak in methanol were found to exhibit the highest TPC (10.27 ± 0.12 mg GAE/g), TFC (11.04 ± 0.89 mg CE/g) and antioxidant properties amongst all samples whereby the TPC and TFC were strongly correlated with antioxidant activities. On the other hand, distilled water extracted Salak kernel showed to have the lowest TPC (0.53 ± 0.05 mg GAE/g), TFC (0.37 ± 0.01 mg CE/g) and antioxidant properties amongst all the Salak extracts. Peel extracts exhibit comparable antioxidant activities with fruit extracts in the current findings. In addition, peel extracts indicated some extend of ACE and alpha-amylase inhibition activities regardless of the solvents used. Methanol and ethanol peel extracts indicated no significant difference (p < 0.05) ACE (98%) and alpha-amylase (90%) inhibition activities. However, distilled water extracted Salak peel showed significantly lower ACE and alpha-amylase inhibition in comparison to methanol and ethanol peel extracts.Originality/valueThe present findings suggested that the fruit of Salak exhibits the highest antioxidant properties, followed by the peel and lastly, the kernel, which shows the lowest antioxidant properties amongst all the samples. The results also indicated that the peel extracts have ACE and alpha-amylase inhibition activities.

scholarly journals Phytochemical Screening and Antioxidant Potentials of Methanol Extract of Duranta erecta Leaves

2020 ◽  
pp. 16-26
Author(s):  
Christopher Chidiebere Ugwu ◽  
Chioma Assumpta Anosike
Keyword(s):  
Nitric Oxide ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Antioxidant Vitamins ◽  
Composition Analysis ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Antioxidant Power

Plant extracts that have antioxidant activities show promising effect in the management of many diseases initiated by oxidative stress. The present study evaluates the phytochemical composition and in vitro antioxidant potentials of methanol extract of Duranta erecta leaves. The extract was subjected to phytochemical screening, DPPH inhibition, ferric reducing antioxidant power (FRAP), nitric oxide determination, antioxidant vitamins and minerals composition analysis. All analyses were carried out using standard biochemical methods. The phytochemical evaluation of the plant extract showed the presence of flavonoids (24.20 ± 0.14 mg QE/g), Alkaloids (15.87 ± 1.71 mg/g), total phenol (12.73 ± 0.61 mg GAE/g), tannins (9.24 ± 0.03 mg TAE/g), terpenoids (8.90 ± 0.96 mg/g), steroids (2.65 ± 0.55 mg/g) and saponins (5.55 ± 0.76 mg/g). The in vitro antioxidant determination showed that the extract had antioxidant properties in a concentration dependent manner. The antioxidant property of the sample was compared to that of ascorbic acid (for DPPH and nitric oxide determination) and gallic acid (for FRAP) as standards. The antioxidant mineral composition of the extract revealed the presence of zinc (1.82 ± 0.03 mg/100g) and selenium (0.59 ± 0.04 mg/100g). The antioxidant vitamins composition of the extract showed moderate concentrations of vitamin C (0.35 ± 0.01 mg/100 g) and vitamin E (0.68 ± 0.07 mg/100g). The result of this study revealed that methanol extract of Duranta erecta leaves could be a good source of antioxidants.

scholarly journals IN VITRO TOTAL PHENOLICS, FLAVONOIDS CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITES OF VARIOUS SOLVENT EXTRACTS FROM THE MEDICINAL PLANT PHYSALIS MINIMA LINN

2017 ◽  
Vol 9 (3) ◽  
pp. 192 ◽  
Author(s):  
Venkanna Banothu ◽  
Uma Adepally ◽  
Jayalakshmi Lingam
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Antioxidant Power

Objective: To estimate the in vitro total phenolics, flavonoids contents, antioxidant and antimicrobial activities of various solvent extracts from the medicinal plant Physalis minima Linn.Methods: The crude bioactive were extracted from the dried powder of Physalis minima using methanol, ethyl acetate, chloroform and hexane solvents. Total phenolic content (TPC) and total flavonoid content (TFC) were estimated using Folin-Ciocalteu and aluminium chloride colorimetric methods respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity. The antimicrobial assay was done through agar well diffusion; minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using broth microdilution methods against the Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris) and Gram-positive bacteria (Staphylococcus aureus).Results: TPC expressed as gallic acid equivalents (GAE) ranged from 60.27±1.73-151.25±2.50 mg GAE/g dry weight, and TFC expressed as quercetin equivalents (QE) ranged from 56.66±0.80-158.84±2.30 mg QE/g dry weight. Methanol extract showed the highest antioxidant activity followed by ethyl acetate, chloroform, hexane extract and the IC50 values of methanol extract for scavenging DPPH and ABTS free radicals were 280.23±5.75-173.40±0.38µg/ml, respectively. All the extracts have shown potent antimicrobial activity for the zone of inhibition ranged from 9-35 mm; MICs and MBCs values ranged from 0.125-4.0 and 0.25-8.0 mg/ml, respectively towards tested pathogenic species.Conclusion: The comprehensive analysis of the present results demonstrated that Physalis minima possess high potential antioxidant properties which could be used as a viable source of natural antioxidants in treating infections caused by above-mentioned pathogens.

scholarly journals UPLC-QTOF-MS/MS and GC-MS Characterization of Phytochemicals in Vegetable Juice Fermented Using Lactic Acid Bacteria from Kimchi and Their Antioxidant Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1761
Author(s):  
Moeun Lee ◽  
Jung Hee Song ◽  
Eun Ji Choi ◽  
Ye-Rang Yun ◽  
Ki Won Lee ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phenyllactic Acid ◽  
Antioxidant Power ◽  
Vegetable Juice ◽  
Daucus Carota L ◽  
Ferric Reducing Antioxidant Power

This study aims to investigate fermentative metabolites in probiotic vegetable juice from four crop varieties (Brassica oleracea var. capitata, B. oleracea var. italica, Daucus carota L., and Beta vulgaris) and their antioxidant properties. Vegetable juice was inoculated with two lactic acid bacteria (LAB) (Companilactobacillus allii WiKim39 and Lactococcus lactis WiKim0124) isolated from kimchi and their properties were evaluated using untargeted UPLC-QTOF-MS/MS and GC-MS. The samples were also evaluated for radical (DPPH• and OH•) scavenging activities, lipid peroxidation, and ferric-reducing antioxidant power. The fermented vegetable juices exhibited high antioxidant activities and increased amounts of total phenolic compounds. Fifteen compounds and thirty-two volatiles were identified using UPLC-QTOF-MS/MS and GC-MS, respectively. LAB fermentation significantly increased the contents of d-leucic acid, indole-3-lactic acid, 3-phenyllactic acid, pyroglutamic acid, γ-aminobutyric acid, and gluconic acid. These six metabolites showed a positive correlation with antioxidant properties. Thus, vegetable juices fermented with WiKim39 and WiKim0124 can be considered as novel bioactive health-promoting sources.

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