scholarly journals A Randomised, Controlled Study of Different Glycaemic Targets during Gestational Diabetes Treatment: Effect on the Level of Adipokines in Cord Blood and ANGPTL4 Expression in Human Umbilical Vein Endothelial Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
P. Popova ◽  
L. Vasilyeva ◽  
A. Tkachuck ◽  
M. Puzanov ◽  
A. Golovkin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Gestational Diabetes ◽  
Cord Blood ◽  
Umbilical Vein ◽  
Fasting Blood Glucose ◽  
Controlled Study ◽  
Control Group ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Our aim was to study the expression of adipokine-encoding genes (leptin, adiponectin, and angiopoietin-like protein 4 (ANGPTL4)) in human umbilical vein endothelial cells (HUVECs) and adipokine concentration in cord blood from women with gestational diabetes mellitus (GDM) depending on glycaemic targets. GDM patients were randomised to 2 groups per target glycaemic levels: GDM1 (tight glycaemic targets, fasting blood glucose < 5.1 mmol/L and <7.0 mmol/L postprandial, N=20) and GDM2 (less tight glycaemic targets, <5.3 mmol/L and < 7.8 mmol/L, respectively, N=21). The control group included 25 women with normal glucose tolerance. ANGPTL4 expression was decreased in the HUVECs from GDM patients versus the control group (23.11 ± 5.71, 21.47 ± 5.64, and 98.33 ± 20.92, for GDM1, GDM2, and controls; p<0.001) with no difference between GDM1 and GDM2. The level of adiponectin gene expression was low and did not differ among the groups. Leptin gene expression was undetectable in HUVECs. In cord blood, leptin/adiponectin ratio (LAR) was increased in GDM2 compared to controls and GDM1 (p=0.038) and did not differ between GDM1 and controls. Tight glycaemic targets were associated with normalisation of increased LAR in the cord blood. ANGPTL4 expression was downregulated in HUVECs of newborns from GDM mothers and was not affected by the intensity of glycaemic control.

scholarly journals Association of tribbles homologue 1 gene expression in human umbilical vein endothelial cells with duration of intrauterine exposure to hyperglycaemia

2018 ◽  
Vol 100 ◽  
Author(s):  
POLINA V. POPOVA ◽  
LIUDMILA B. VASILEVA ◽  
ALEXANDRA S. TKACHUK ◽  
MAXIM V. PUZANOV ◽  
YANA A. BOLOTKO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Control Group ◽  
Control Groups ◽  
Human Umbilical Vein ◽  
Intrauterine Exposure ◽  
The Difference ◽  
Umbilical Vein Endothelial Cells ◽  
And Control

SummaryMaternal gestational diabetes mellitus (GDM) is considered to be an important factor that epigenetically predisposes offspring to metabolic and cardiovascular diseases. However, the mechanisms of how intrauterine hyperglycaemia affects offspring have not been thoroughly studied. The mammalian tribbles homologue 1 (TRIB1) gene is associated with plasma lipid concentrations and coronary artery disease (CAD). Our aim was to study the effect of GDM and its treatment terms on the level of TRIB1 gene expression in human umbilical vein endothelial cells (HUVECs) of newborns from women with and without GDM. The study included 50 women with GDM and 25 women without GDM (control group). Women with GDM were divided into three groups according to their gestational age when the treatment of GDM started: 24–28 weeks (GDM1, N = 16), 29–32 weeks (GDM2, N = 25) and >34 weeks (GDM3, N = 9). The levels of TRIB1 gene expression in GDM3, GDM2, GDM1 and control groups were 2.8 ± 1.1, 4.2 ± 2.4, 6.0 ± 3.4 and 8.1 ± 6.1, respectively (p = 0.001). After comparison in pairs the difference was significant for the following pairs: GDM2–control (p = 0.004), GDM3–control (p = 0.002), GDM1–GDM3 (p = 0.012). Notably, if treatment had been started before the 28th week of gestation, the difference in TRIB1 gene expression in HUVECs was not significant (p = 0.320 for comparison between GDM1 and control groups). Our findings support the hypothesis that TRIB1 gene expression in HUVECs depends on the duration of intrauterine exposure to hyperglycaemia.

scholarly journals Dengue Virus Induces Novel Changes in Gene Expression of Human Umbilical Vein Endothelial Cells

2003 ◽  
Vol 77 (21) ◽  
pp. 11822-11832 ◽  
Author(s):  
Rajas V. Warke ◽  
Kris Xhaja ◽  
Katherine J. Martin ◽  
Marcia F. Fournier ◽  
Sunil K. Shaw ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Dengue Virus ◽  
Immune Cell ◽  
Umbilical Vein ◽  
Oligoadenylate Synthetase ◽  
Functional Responses ◽  
Human Umbilical Vein ◽  
Phospholipid Scramblase ◽  
Umbilical Vein Endothelial Cells

ABSTRACT Endothelial cells are permissive to dengue virus (DV) infection in vitro, although their importance as targets of DV infection in vivo remains a subject of debate. To analyze the virus-host interaction, we studied the effect of DV infection on gene expression in human umbilical vein endothelial cells (HUVECs) by using differential display reverse transcription-PCR (DD-RTPCR), quantitative RT-PCR, and Affymetrix oligonucleotide microarrays. DD identified eight differentially expressed cDNAs, including inhibitor of apoptosis-1, 2′-5′ oligoadenylate synthetase (OAS), a 2′-5′ OAS-like (OASL) gene, galectin-9, myxovirus protein A (MxA), regulator of G-protein signaling, endothelial and smooth muscle cell-derived neuropilin-like protein, and phospholipid scramblase 1. Microarray analysis of 22,000 human genes confirmed these findings and identified an additional 269 genes that were induced and 126 that were repressed more than fourfold after DV infection. Broad functional responses that were activated included the stress, defense, immune, cell adhesion, wounding, inflammatory, and antiviral pathways. These changes in gene expression were seen after infection of HUVECs with either laboratory-adapted virus or with virus isolated directly from plasma of DV-infected patients. Tumor necrosis factor alpha, OASL, and MxA and h-IAP1 genes were induced within the first 8 to 12 h after infection, suggesting a direct effect of DV infection. These global analyses of DV effects on cellular gene expression identify potentially novel mechanisms involved in dengue disease manifestations such as hemostatic disturbance.

scholarly journals In vitro effects of low-level aldehyde exposures on human umbilical vein endothelial cells

2015 ◽  
Vol 4 (5) ◽  
pp. 1250-1259 ◽  
Author(s):  
Nuan P. Cheah ◽  
Jeroen L.A. Pennings ◽  
Jolanda P. Vermeulen ◽  
Roger W.L. Godschalk ◽  
Frederik J. van Schooten ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cardiovascular Disease ◽  
Endothelial Cells ◽  
Tobacco Smoke ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
In Vitro Effects ◽  
Umbilical Vein Endothelial Cells ◽  
Disease Exposure

Aldehydes cause gene expression changes for genes associated with cardiovascular disease. Exposure to aldehydes from tobacco smoke needs to be controlled.

Comparative analysis of effects of cyclic uniaxial and equiaxial stretches on gene expression of human umbilical vein endothelial cells

2015 ◽  
Vol 39 (6) ◽  
pp. 741-749 ◽  
Author(s):  
Shahrokh Shojaei ◽  
Mohammad Tafazzoli-Shahdpour ◽  
Mohammad Ali Shokrgozar ◽  
Nooshin Haghighipour
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Comparative Analysis ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

The Influence of Cyclic and Uniform Shear Stresses Concurrent with Cyclic Stretch on the Gene Expression of Human Umbilical Vein Endothelial Cells

2013 ◽  
Vol 3 (6) ◽  
pp. 673-678 ◽  
Author(s):  
Shahrokh Shojaei ◽  
Mohammad Tafazzoli-Shadpour ◽  
Mohammad Ali Shokrgozar ◽  
Nooshin Haghighipour ◽  
Najmeh Safaei ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Cyclic Stretch ◽  
Shear Stresses ◽  
Human Umbilical Vein ◽  
Uniform Shear ◽  
Umbilical Vein Endothelial Cells
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