scholarly journals Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Dina Sibouakaz ◽  
Khira Othmani-Mecif ◽  
Amirouche Fernane ◽  
Abdennour Taghlit ◽  
Yasmina Benazzoug
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Negative Impact ◽  
Oxidant Stress ◽  
High Fat ◽  
Plasma Parameters ◽  
Male And Female ◽  
Heart Ultrastructure ◽  
Early Weight Gain ◽  
Stress Parameters

Early weight gain induced by high-fat diet has been identified as a predictor for cardiac disease, one of the most serious public health problems. Our goal is to study the influence of a HFD on biochemical, oxidant stress parameters, and the cardiac ultrastructure in both male and female prepubertal models. Experiments were carried on 24 prepubertal New Zealand white rabbits, randomly assigned to male and female control (MC and FC, resp.) or HFD (MHFD and FHFD, resp.) groups (n=6) for 3 months. Body and heart weights and some biochemical and oxidative stress parameters such as lipids, calcium, CKMB, MDA, uric acid, ascorbic acid, and AOA are evaluated in plasma and the left ventricle. Under HFD effect, plasma parameters, such as lipids (TL, PL, and LDL-C), MDA, and CK-MB, increase more significantly in male than in female groups, when AA decreases. Some cardiac parameters such as TG and UA increase, when AA and AOA decrease; these variations are more significant in FHFD. In both male and female rabbits, HFD caused changes in heart ultrastructure, junctional complexes, mitochondria size and form, and so on. Early HFD feeding induced overweight, oxidative stress, and metabolic alterations in plasma and the heart of prepubertal rabbits, whereas lipotoxicity has especially a negative impact on male plasma but affects more the female heart ultrastructure.

Sex-dependent effect on mitochondrial and oxidative stress parameters in the hypothalamus induced by prepubertal stress and access to high fat diet

2019 ◽  
Vol 124 ◽  
pp. 114-122 ◽  
Author(s):  
Ana Paula Toniazzo ◽  
Danusa Mar Arcego ◽  
Camilla Lazzaretti ◽  
Carina Mota ◽  
Carlos Eduardo Schnorr ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
High Fat ◽  
Dependent Effect ◽  
Oxidative Stress Parameters ◽  
And Oxidative Stress ◽  
Stress Parameters

An experimental MODEL study to investigate the pathogenesis of oxaliplatin-induced liver injury.

2013 ◽  
Vol 31 (4_suppl) ◽  
pp. 184-184
Author(s):  
Stuart M. Robinson ◽  
Jelena Mann ◽  
Derek M. Manas ◽  
Derek A. Mann ◽  
Steve A. White
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
High Fat Diet ◽  
Therapeutic Potential ◽  
Negative Impact ◽  
Acid Synthesis ◽  
Sinusoidal Obstruction Syndrome ◽  
High Fat ◽  
Expression Of Genes ◽  
Folfox Chemotherapy

184 Background: Oxaliplatin based chemotherapy is widely utilized pre-operatively in patients with colorectal liver metastases. Its use is associated with injury to the liver in the form of sinusoidal obstruction syndrome (SOS) the presence of which can have a negative impact on surgical outcomes. The pathogenesis of this condition is poorly understood. Methods: C57Bl/6 mice (n=10 per group) were treated with 5-FU/oxaliplatin/folinic acid chemotherapy (FOLFOX) weekly for 5 weeks or their respective vehicle controls. Animals were culled one week following the final treatment and liver tissue harvested for histological and biochemical analysis. mRNA was extracted from snap frozen liver and subject to genome wide expression analysis the results of which were confirmed using qRT-PCR. To determine the effect of background steatosis on the development of liver injury the experiment was repeated using mice maintained on a high fat diet. To assess the ability of antioxidants to prevent SOS development diet was supplemented with 3% butylated hydroxyanisole (BHA). Results: H&E stained tissue sections confirmed the presence of SOS in all FOLFOX treated animals. Microarray identified changes in expression of over 604 genes in the liver of animals with FOLFOX induced SOS. In particular there was increased expression of genes implicated in oxidative stress (e.g. Metallothionein 1; 22 fold; p<0.001), cell cycle arrest (e.g. p21; 21 fold; p<0.001) and angiogenesis (VEGF-A; 2 fold; p<0.001). Administration of the antioxidant BHA alongside chemotherapy prevented the development of SOS confirming the role of oxidative stress in the pathogenesis of this condition. There was down regulation of a number of genes implicated in fatty acid synthesis (e.g. FASN; 8 fold; p<0.001). In support of this mice maintained on a high fat diet treated with FOLFOX do not develop steatosis unlike those treated with vehicle alone. Conclusions: FOLFOX chemotherapy is specifically associated with SOS and not hepatic steatosis. We have identified some of the molecular pathways involved in the pathogenesis of this condition. Manipulation of these pathways may be of therapeutic potential in preventing the development of chemotherapy-associated liver injury.

scholarly journals Nigella sativaRelieves the Altered Insulin Receptor Signaling in Streptozotocin-Induced Diabetic Rats Fed with a High-Fat Diet

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Mahmoud Balbaa ◽  
Marwa El-Zeftawy ◽  
Doaa Ghareeb ◽  
Nabil Taha ◽  
Abdel Wahab Mandour
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Blood Glucose ◽  
Insulin Receptor ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Diabetic Rats ◽  
High Fat ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

The black cumin (Nigella sativa) “NS” or the black seeds have many pharmacological activities such as antioxidant, anticarcinogenic, antihypertensive, and antidiabetic properties. In this work, streptozotocin-induced diabetic rats fed with a high-fat diet were treated daily with NS oil (NSO) in order to study the effect on the blood glucose, lipid profile, oxidative stress parameters, and the gene expression of some insulin receptor-induced signaling molecules. This treatment was combined also with some drugs (metformin and glimepiride) and the insulin receptor inhibitor I-OMe-AG538. The administration of NSO significantly induced the gene expression of insulin receptor compared to rats that did not receive NSO. Also, it upregulated the expression of insulin-like growth factor-1 and phosphoinositide-3 kinase, whereas the expression of ADAM-17 was downregulated. The expression of ADAM-17 is corroborated by the analysis of TIMP-3 content. In addition, the NSO significantly reduced blood glucose level, components of the lipid profile, oxidative stress parameters, serum insulin/insulin receptor ratio, and the tumor necrosis factor-α, confirming that NSO has an antidiabetic activity. Thus, the daily NSO treatment in our rat model indicates that NSO has a potential in the management of diabetes as well as improvement of insulin-induced signaling.

scholarly journals Relationship between exercise and high fat diet with oxidative stress parameters in the immune system

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Beatriz Elina Martinez-Carillo ◽  
Roxana Valdés-Ramos ◽  
Ana Laura Guadarrama ◽  
Alexandre Kormanovski
Keyword(s):  
Oxidative Stress ◽  
Immune System ◽  
High Fat Diet ◽  
High Fat ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

Effects of Lentinula edodes consumption on biochemical, hematologic and oxidative stress parameters in rats receiving high-fat diet

2016 ◽  
Vol 56 (7) ◽  
pp. 2255-2264 ◽  
Author(s):  
Sara Rosicler Vieira Spim ◽  
Bruna Giovanna Corrêa Chrispim de Oliveira ◽  
Fernanda Gomes Leite ◽  
Marli Gerenutti ◽  
Denise Grotto
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Lentinula Edodes ◽  
High Fat ◽  
Oxidative Stress Parameters ◽  
And Oxidative Stress ◽  
Stress Parameters

Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD

2021 ◽  
Author(s):  
Jingda Li ◽  
Tianqi Wang ◽  
Panpan Liu ◽  
Fuyuan Yang ◽  
Xudong Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oleic Acid ◽  
Rat Model ◽  
High Fat Diet ◽  
Hepg2 Cells ◽  
Citrus Fruits ◽  
High Fat ◽  
Hepatic Oxidative Stress

Hesperetin as a major bioflavonoid in citrus fruits improves NAFLD by suppressing hepatic oxidative stress and inflammation.

scholarly journals High-fat diet-induced obesity and impairment of brain neurotransmitter pool

2020 ◽  
Vol 11 (1) ◽  
pp. 147-160
Author(s):  
Ranyah Shaker M. Labban ◽  
Hanan Alfawaz ◽  
Ahmed T. Almnaizel ◽  
Wail M. Hassan ◽  
Ramesa Shafi Bhat ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Brain Tissue ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Obese Group ◽  
Control Group ◽  
High Fat ◽  
Brain Neurotransmitter ◽  
The Brain ◽  
Lean Group

AbstractObesity and the brain are linked since the brain can control the weight of the body through its neurotransmitters. The aim of the present study was to investigate the effect of high-fat diet (HFD)-induced obesity on brain functioning through the measurement of brain glutamate, dopamine, and serotonin metabolic pools. In the present study, two groups of rats served as subjects. Group 1 was fed a normal diet and named as the lean group. Group 2 was fed an HFD for 4 weeks and named as the obese group. Markers of oxidative stress (malondialdehyde, glutathione, glutathione-s-transferase, and vitamin C), inflammatory cytokines (interleukin [IL]-6 and IL-12), and leptin along with a lipid profile (cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein levels) were measured in the serum. Neurotransmitters dopamine, serotonin, and glutamate were measured in brain tissue. Fecal samples were collected for observing changes in gut flora. In brain tissue, significantly high levels of dopamine and glutamate as well as significantly low levels of serotonin were found in the obese group compared to those in the lean group (P > 0.001) and were discussed in relation to the biochemical profile in the serum. It was also noted that the HFD affected bacterial gut composition in comparison to the control group with gram-positive cocci dominance in the control group compared to obese. The results of the present study confirm that obesity is linked to inflammation, oxidative stress, dyslipidemic processes, and altered brain neurotransmitter levels that can cause obesity-related neuropsychiatric complications.

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