scholarly journals Effects of Fine Particulate Matter onPseudomonas aeruginosaAdhesion and Biofilm FormationIn Vitro

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Seon Hee Woo ◽  
Sang Moog Lee ◽  
Ki Cheol Park ◽  
Gyeong Nam Park ◽  
Byeolnimhee Cho ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Epithelial Cells ◽  
Biofilm Formation ◽  
Protein Concentration ◽  
Fine Particulate Matter ◽  
Surface Hydrophobicity ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Surface ◽  
Fine Particulate

Respiratory infections ofPseudomonas aeruginosaare a major cause of mortality and morbidity for hospitalized patients. Fine particulate matter (FPM) is known to have interactions with some bacterial infection in the respiratory system. In this report, we investigate the effect of different concentration of FPM onP. aeruginosaattachment and biofilm formation usingin vitrocell culture systems.P. aeruginosawere cultured to form mature biofilms on hydroxyapatite-coated peg and the number of bacteria in the biofilms was enumerated. Morphology of biofilm was imaged with scanning electron microscopy and confocal laser scanning microscopy. Bacterial affinity change to the cell membrane was evaluated with attached colony counting and fluorescence microscopy images. Alteration of bacterial surface hydrophobicity and S100A4 protein concentration were explored as mechanisms ofP. aeruginosaadhesion to human cells. There were a concentration-dependent increase of thickness and surface roughness of biofilm mass.P. aeruginosaadherence to respiratory epithelial cells was increased after FPM treatment. Bacterial surface hydrophobicity and S1000A4 protein concentration were increased with proportionally the dose of FPM in media. FPM in the airway could enhance both the adhesion ofP. aeruginosato epithelial cells and biofilm formation. Bacterial surface hydrophobicity and human cell plasma membrane injury are associated with binding ofP. aeruginosaon airway epithelial cells and biofilm formation.

scholarly journals Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells

2020 ◽  
Vol 7 ◽  
pp. 859-866
Author(s):  
Nobuyuki Yamagishi ◽  
Tomoki Yamaguchi ◽  
Takahisa Kuga ◽  
Masanari Taniguchi ◽  
Mohammad Shahriar Khan ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Fine Particulate Matter ◽  
Tracheal Epithelial Cells ◽  
Fine Particulate ◽  
Tracheal Epithelial

scholarly journals Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zhiyan He ◽  
Xu Zhang ◽  
Zhongchen Song ◽  
Lu Li ◽  
Haishuang Chang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Periodontal Disease ◽  
Biofilm Formation ◽  
Cell Structure ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Antimicrobial Effects ◽  
Virulence Properties

Abstract Porphyromonas gingivalis is a causative agent in the onset and progression of periodontal disease. This study aims to investigate the effects of quercetin, a natural plant product, on P. gingivalis virulence properties including gingipain, haemagglutinin and biofilm formation. Antimicrobial effects and morphological changes of quercetin on P. gingivalis were detected. The effects of quercetin on gingipains activities and hemolytic, hemagglutination activities were evaluated using chromogenic peptides and sheep erythrocytes. The biofilm biomass and metabolism with different concentrations of quercetin were assessed by the crystal violet and MTT assay. The structures and thickness of the biofilms were observed by confocal laser scanning microscopy. Bacterial cell surface properties including cell surface hydrophobicity and aggregation were also evaluated. The mRNA expression of virulence and iron/heme utilization was assessed using real time-PCR. Quercetin exhibited antimicrobial effects and damaged the cell structure. Quercetin can inhibit gingipains, hemolytic, hemagglutination activities and biofilm formation at sub-MIC concentrations. Molecular docking analysis further indicated that quercetin can interact with gingipains. The biofilm became sparser and thinner after quercetin treatment. Quercetin also modulate cell surface hydrophobicity and aggregation. Expression of the genes tested was down-regulated in the presence of quercetin. In conclusion, our study demonstrated that quercetin inhibited various virulence factors of P. gingivalis.

scholarly journals Mosaic-CLSM Assessment of Bacterial Spatial Distribution in Cosmetic Matrices According to Matrix Viscosity and Bacterial Hydrophobicity

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 32
Author(s):  
Samia Almoughrabie ◽  
Chrisse Ngari ◽  
Romain Briandet ◽  
Valérie Poulet ◽  
Florence Dubois-Brissonnet
Keyword(s):  
Spatial Distribution ◽  
Laser Scanning ◽  
Rapid Assessment ◽  
Challenge Test ◽  
Surface Hydrophobicity ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Surface ◽  
Bacterial Hydrophobicity ◽  
The Impact

The reliability of the challenge test depends, among other parameters, on the spatial distribution of microorganisms in the matrix. The present study aims to quickly identify factors that are susceptible to impair a uniform distribution of inoculated bacteria in cosmetic matrices in this context. We used mosaic confocal laser scanning microscopy (M-CLSM) to obtain rapid assessment of the impact of the composition and viscosity of cosmetic matrices on S. aureus spatial distribution. Several models of cosmetic matrices were formulated with different concentrations of two thickeners and were inoculated with three S. aureus strains having different levels of hydrophobicity. The spatial distribution of S. aureus in each matrix was evaluated according to the frequency distribution of the fluorescence values of at least 1350 CLSM images. We showed that, whatever the thickener used, an increasingly concentration of thickener results in increasingly bacterial clustered distribution. Moreover, higher bacterial hydrophobicity also resulted in a more clustered spatial distribution. In conclusion, CLSM-based method allows a rapid characterization of bacterial spatial distribution in complex emulsified systems. Both matrix viscosity and bacterial surface hydrophobicity affect the bacterial spatial distribution which can have an impact on the reliability of bacterial enumeration during challenge test.

scholarly journals Development of a system for the detection of the inflammatory response induced by airborne fine particulate matter in rat tracheal epithelial cells

2020 ◽  
Vol 7 ◽  
pp. 900-908 ◽  
Author(s):  
Nobuyuki Yamagishi ◽  
Tomoki Yamaguchi ◽  
Takahisa Kuga ◽  
Masanari Taniguchi ◽  
Mohammad Shahriar Khan ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Fine Particulate Matter ◽  
Tracheal Epithelial Cells ◽  
Fine Particulate ◽  
Tracheal Epithelial
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