scholarly journals Preparation of Ampicillin Surface Molecularly Imprinted Polymers for Its Selective Recognition of Ampicillin in Eggs Samples

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yang Tian ◽  
Yue Wang ◽  
Shanshan Wu ◽  
Zhian Sun ◽  
Bolin Gong
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Selective Adsorption ◽  
Water Soluble ◽  
Penicillin G ◽  
Scatchard Analysis ◽  
Template Molecule ◽  
Phase Extraction ◽  
Imprinted Polymers ◽  
Relative Standard

Surface-imprinted polymers (MIPs) microspheres with the ability to specifically recognize water-soluble molecules were prepared using self-made monodisperse porous poly(chloromethylstyrene-co-divinylbenzene) beads as the solid-phase matrix and ampicillin (AMP) as the template molecule. MIPs were synthesized using different template molecule: monomer: crosslinker ratios and the optimum preparation ratio were obtained by measuring adsorption. The maximum equilibrium amount of adsorption by the MIPs reached 115.62 mg/g. Scatchard analysis indicated that the MIPs contained two types of recognition sites: specific and nonspecific. Based on the adsorption kinetics, adsorption equilibrium was reached after 30 minutes. Penicillin G, amoxicillin, and sulbactam acid were used as competitive molecules to research the selective adsorption capacity of the MIPs. The imprinted material was found to have good selectivity with selectivity coefficients for penicillin G, amoxicillin, and sulbactam acid of 5.74, 6.83, and 7.25, respectively. The MIPs were used as solid-phase extraction filler, resulting in successful enrichment and separation of ampicillin residue from egg samples. Standard addition recovery experiments revealed that recovery was good with recoveries from the spiked samples ranging from 91.5 to 94.9% and relative standard deviations from 3.6 to 4.2%. The solid-phase extraction MIPs microcolumn was reused 10 times, where it maintained a recovery rate of over 80%. This work presents a sensitive, fast, and convenient method for the determination of trace ampicillin in food samples.

scholarly journals Synergetic Effect of Dual Functional Monomers in Molecularly Imprinted Polymer Preparation for Selective Solid Phase Extraction of Ciprofloxacin

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2788
Author(s):  
Ut Dong Thach ◽  
Hong Hanh Nguyen Thi ◽  
Tuan Dung Pham ◽  
Hong Dao Mai ◽  
Tran-Thi Nhu-Trang
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Template Molecule ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Dual Functional

Background: Ciprofloxacin (CIP), an important broad-spectrum fluoroquinolone antibiotic, was often used as a template molecule for the preparation of imprinted materials. In this study, methacrylic acid and 2-vinylpyridine were employed for the first time as dual functional monomers for synthesizing ciprofloxacin imprinted polymers. Methods: The chemical and physicochemical properties of synthesized polymers were characterized using Fourier transform-infrared spectroscopy, thermogravimetric analysis-differential scanning calorimetry, scanning electron microscopy, and nitrogen adsorption-desorption isotherm. The adsorption properties of ciprofloxacin onto synthesized polymers were determined by batch experiments. The extraction performances were studied using the solid phase extraction and HPCL-UV method. Results: The molecularly imprinted polymer synthesized with dual functional monomers showed a higher adsorption capacity and selectivity toward the template molecule. The adsorbed amounts of ciprofloxacin onto the imprinted and non-imprinted polymer were 2.40 and 1.45 mg g−1, respectively. Furthermore, the imprinted polymers were employed as a selective adsorbent for the solid phase extraction of ciprofloxacin in aqueous solutions with the recovery of 105% and relative standard deviation of 7.9%. This work provides an alternative approach for designing a new adsorbent with high adsorption capacity and good extraction performance for highly polar template molecules.

scholarly journals Targeted extraction of active compounds from natural products by molecularly imprinted polymers

2012 ◽  
Vol 10 (3) ◽  
pp. 751-765 ◽  
Author(s):  
Antonelle Pardo ◽  
Laetitia Mespouille ◽  
Philippe Dubois ◽  
Pierre Duez ◽  
Bertrand Blankert
Keyword(s):  
Natural Products ◽  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymers ◽  
High Efficiency ◽  
Solid Phase ◽  
Template Molecule ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Therapeutic Benefits

AbstractOne of the most promising separation techniques that have emerged during the last decade is based on the use of molecularly imprinted polymers (MIPs). MIPs are stable polymers that possess specific cavities designed for a template molecule, endowed with excellent selectivity compared to regular solid phase extraction techniques. Molecularly imprinted solid-phase extraction (MISPE) has already shown a high efficiency for the sample preparation from complex matrices. Natural products received huge attention in recent years. Indeed, the application of MISPE for the screening of natural products appears extremely interesting not only for the selective extraction of a target compound but also for the concomitant discovery of new drug candidates, promising sources of therapeutic benefits. In the present review, examples of recognition and separation of active components from natural extracts are emphasized. MIPs are very promising materials to mimic the recognition characteristics exhibited by enzymes or receptors although further developments are necessary to fully exploit their wide potential.

scholarly journals Application of Molecular Imprinted Polymers for Selective Solid Phase Extraction of Bisphenol A

2016 ◽  
Vol 23 (4) ◽  
pp. 651-664 ◽  
Author(s):  
Anna Poliwoda ◽  
Małgorzata Mościpan ◽  
Piotr P. Wieczorek
Keyword(s):  
Bisphenol A ◽  
Solid Phase Extraction ◽  
Methacrylic Acid ◽  
Solid Phase ◽  
Selective Extraction ◽  
Phase Extraction ◽  
Aqueous Samples ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Relative Standard

Abstract Selective molecularly imprinted polymers (MIPs) with bisphenol A as template were synthesized using the non-covalent imprinting approach. MIPs were prepared using thermally initiated polymerization with 1,1’-azobis(cyclohexanecarbonitryle) (ACHN) as initiator and ethylene glycol dimethacrylate (EDMA) as a cross-linking agent. The tested functional monomers included methacrylic acid, acrylamide, and 4-vinylpyridine. The selectivity of the BPA-MIP for the solid phase extraction of bisphenol A was tested in samples containing other related alkylphenols. The polymers prepared in acetonitrile using methacrylic acid or acrylamide as monomer showed the highest selectivity towards target analyte (the selectivity ratio 8:1, respectively for MIP and NIP). The proposed procedure has been proven to be an effective for selective extraction of bisphenol A in aqueous samples (recoveries over 85%) enabling detection and quantification limits of 25 and 70 μg/dm3, respectively based on 10 cm3 of sample volume, with relative standard deviations (RSD) lower than 6%. The obtained molecularly imprinted material showed interesting properties for selective extraction and preconcentration of studied analyte from large volumes of aqueous samples without any problems of cartridge clogging.

scholarly journals Determination of Penicillin G in Feeds by Liquid Chromatography with Solid-Phase Extraction

2005 ◽  
Vol 88 (3) ◽  
pp. 679-683 ◽  
Author(s):  
Matthew J Gramse ◽  
Paul E Jacobson
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Reversed Phase ◽  
Penicillin G ◽  
Phase Extraction ◽  
Solid Phase Extraction Cartridge ◽  
Relative Standard ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method was developed for the determination of penicillin G in feeds. The method involves extraction of penicillin G with methanol, concentration under a stream of nitrogen, and cleanup using Phenomenex Strata-X solid-phase extraction cartridge. Analyte separation and quantification were achieved by gradient reversed-phase liquid chromatography and ultraviolet absorbance at 230 nm. Average spike recoveries for samples prepared at 3 spiking levels (25, 50, and 200 g/ton) were 96.3, 92.1, and 88.6%, respectively. The overall method precision at each of the 3 spiking levels was ≤5.39% relative standard deviation. The limits of detection and quantititation (g/ton formulation) were 3.89 and 13.0 g/ton, respectively.

Fibrous g-C3N4@Tio2 Nanocomposites-Based Dispersive Micro-Solid Phase Extraction for Chromium Speciation in Cow Milk by ICP-MS after Digestion Treatment with Artificial Gastric Juice

2020 ◽  
Author(s):  
Shizhong Chen ◽  
Yuxiu Liu ◽  
Juntao Yan ◽  
Chunlei Wang ◽  
Dengbo Lu
Keyword(s):  
Solid Phase Extraction ◽  
Gastric Juice ◽  
Solid Phase ◽  
Selective Adsorption ◽  
Cow Milk ◽  
Phase Extraction ◽  
Digestion Solution ◽  
Icp Ms ◽  
Relative Standard ◽  
Artificial Gastric Juice

Abstract Background Chromium is an interesting element because its toxicity depends on its speciation. Thus, knowledge of Cr speciation in cow milk is essential to human health. Objective This study aims to achieve real bioaccessible species in cow milk, including Cr(III), Cr(VI), residual, digestible, and total Cr. Methods Samples were treated with artificial gastric juice, followed by dispersive micro-solid phase extraction (DMSPE) combined with ICP-MS for Cr speciation. Fibrous g-C3N4@TiO2 nanocomposites (FGCTNCs) were used as a novel adsorbent for DMSPE. Results The method detection limits were 110 pg/g (Cr(III)) and 260 pg/g (Cr(VI)) for milk powder (0.1 g), and 5.1 pg/g (Cr(III)) and 13 pg/g (Cr(VI)) for liquid cow milk (2 mL). The relative standard deviations (RSDs), obtained by analyzing the standard solutions containing 1.0 ng/mL of the analytes in sequence for nine times, were 4.3% and 5.1% for Cr(III) and Cr(VI), respectively. Linearity was observed over the range of 4 magnitude orders with correlation coefficients better than 0.9961. The enrichment factor of 100 was obtained. The majority of Cr in the samples was transferred into digestion solution. The content of Cr(III) is much higher than that of Cr(VI) in the digestion solution. Conclusions This method has the advantages of reduced solvent consumption, less adsorbent dosage, and high extraction efficiency. It may become a valuable strategy for elemental species in food samples. Highlights The samples were treated with artificial gastric juice to avoid the inter-conversion of species. FGCTNCs exhibit the merits of N-rich functional groups and selective adsorption for the analytes.

Preparation of Roxarsone Imprinted Polymers and Its Application to Solid Phase Extraction

2013 ◽  
Vol 41 (9) ◽  
pp. 1401
Author(s):  
Gao-Kui ZHANG ◽  
Jian-Wen YANG ◽  
Zong-Nan WANG ◽  
Kui BIAN ◽  
Hai-Cui YANG ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymers

Factorial Design Optimisation of Solid Phase Extraction for Preconcentration of Parabens in Wastewater Using Ultra-High Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry

2020 ◽  
Vol 16 (4) ◽  
pp. 436-446
Author(s):  
Vallerie A. Muckoya ◽  
Philiswa N. Nomngongo ◽  
Jane C. Ngila
Keyword(s):  
Solid Phase Extraction ◽  
Formic Acid ◽  
Factorial Design ◽  
Solid Phase ◽  
Treatment Plant ◽  
Phase Extraction ◽  
Limit Of Quantification ◽  
Analytical Technique ◽  
Relative Standard ◽  
Simulated Wastewater

Background: Parabens are synthetic esters used extensively as preservatives and/or bactericides in personal care personal products. Objective: Development and validation of a novel robust chemometric assisted analytical technique with superior analytical performances for the determination of ethylparaben, methylparaben and propylparaben, using simulated wastewater matrix. Methods: An automated Solid Phase Extraction (SPE) method coupled with liquid chromatographymass spectrometry was applied in this study. A gradient elution programme comprising of 0.1% formic acid in deionised water (A) and 0.1% formic acid in Methanol (B) was employed on a 100 x 2.1 mm, 3.0 μm a particle size biphenyl column. Two-level (2k) full factorial design coupled with response surface methodology was used for optimisation and investigation of SPE experimental variables that had the most significant outcome of the analytical response. Results: According to the analysis of variance (ANOVA), sample pH and eluent volume were statistically the most significant parameters. The method developed was validated for accuracy, precision, Limits of Detection (LOD) and Limit of Quantification (LOQ) and linearity. The LOD and LOQ established under those optimised conditions varied between 0.04-0.12 μgL−1 and 0.14-0.40 μgL−1 respectively. The use of matrix-matched external calibration provided extraction recoveries between 78-128% with relative standard deviations at 2-11% for two spike levels (10 and 100 μgL-1) in three different water matrices (simulated wastewater, influent and effluent water). Conclusion: The newly developed method was applied successfully to the analyses of parabens in wastewater samples at different sampling points of a wastewater treatment plant, revealing concentrations of up to 3 μgL−1.

Green Dispersive Micro Solid-Phase Extraction using Multiwalled Carbon Nanotubes for Preconcentration and Determination of Cadmium and Lead in Food, Water, and Tobacco Samples

2020 ◽  
Vol 16 (4) ◽  
pp. 381-392
Author(s):  
Ayman A. Gouda ◽  
Ali H. Amin ◽  
Ibrahim S. Ali ◽  
Zakia Al Malah
Keyword(s):  
Carbon Nanotubes ◽  
Solid Phase Extraction ◽  
Multiwalled Carbon Nanotubes ◽  
Solid Phase ◽  
Certified Reference Materials ◽  
Phase Extraction ◽  
Multiwalled Carbon ◽  
Flame Atomic Absorption ◽  
Relative Standard ◽  
Separation And Preconcentration

Background: Cadmium (Cd2+) and lead (Pb2+) have acute and chronic effects on humans and other living organisms. In the present work, new, green and accurate dispersive micro solid-phase extraction (DμSPE) method for the separation and preconcentration of trace amounts of cadmium (Cd2+) and lead (Pb2+) ions in various food, water and tobacco samples collected from Saudi Arabia prior to its Flame Atomic Absorption Spectrometric (FAAS) determinations was developed. Methods: The proposed method was based on a combination of oxidized multiwalled carbon nanotubes (O-MWCNTs) with a new chelating agent 5-benzyl-4-[4-methoxybenzylideneamino)-4H- 1,2,4-triazole-3-thiol (BMBATT) to enrich and separate trace levels of Cd2+ and Pb2+. The effect of separation parameters was investigated. The validation of the proposed preconcentration procedure was performed using certified reference materials. Results: Analyte recovery values ranged from 95-102%, indicating that the method is highly accurate. Furthermore, precision was demonstrated by the relative standard deviation (RSD < 3.0%). The limits of detection were 0.08 and 0.1 μg L−1 for Cd2+ and Pb2+ ions, respectively. The preconcentration factor was 200. Conclusion: The proposed method was used for the estimation of Cd2+ and Pb2+ ion content in various real samples, and satisfactory results were obtained. The proposed method has high adsorption capacity, rapid adsorption equilibrium, extremely low LODs, high preconcentration factors and shortens the time of sample preparation in comparison to classical SPE.

Highly selective enrichment and direct determination of imazethapyr residues from milk using magnetic solid-phase extraction based on restricted-access molecularly imprinted polymers

2021 ◽  
Author(s):  
Shicong Jia ◽  
yanqiang zhou ◽  
Jianmin Li ◽  
Bolin Gong ◽  
Shujuan Ma ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Direct Determination ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
Restricted Access ◽  
Imprinted Polymers ◽  
Magnetic Solid

The restricted access media magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared as magnetic solid phase extraction (M-SPE) material by reversible addition fragmentation chain transfer (RAFT) technique. The resulting RAM-MMIPs had...

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