scholarly journals Persistence of Brucella abortus in the Bone Marrow of Infected Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Cristina Gutiérrez-Jiménez ◽  
Lisiena Hysenaj ◽  
Alejandro Alfaro-Alarcón ◽  
Ricardo Mora-Cartín ◽  
Vilma Arce-Gorvel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Model ◽  
Bacterial Infection ◽  
Acute Phase ◽  
Brucella Abortus ◽  
Mononuclear Phagocyte ◽  
Trojan Horse ◽  
Mononuclear Phagocyte System ◽  
Histopathological Changes ◽  
Chronic Infections

Brucellosis is a zoonotic bacterial infection that may persist for long periods causing relapses in antibiotic-treated patients. The ability of Brucella to develop chronic infections is linked to their capacity to invade and replicate within the mononuclear phagocyte system, including the bone marrow (BM). Persistence of Brucella in the BM has been associated with hematological complications such as neutropenia, thrombocytopenia, anemia, and pancytopenia in human patients. In the mouse model, we observed that the number of Brucella abortus in the BM remained constant for up to 168 days of postinfection. This persistence was associated with histopathological changes, accompanied by augmented numbers of BM myeloid GMP progenitors, PMNs, and CD4+ lymphocytes during the acute phase (eight days) of the infection in the BM. Monocytes, PMNs, and GMP cells were identified as the cells harboring Brucella in the BM. We propose that the BM is an essential niche for the bacterium to establish long-lasting infections and that infected PMNs may serve as vehicles for dispersion of Brucella organisms, following the Trojan horse hypothesis. Monocytes are solid candidates for Brucella reservoirs in the BM.

scholarly journals A mouse model of HIES reveals pro- and anti-inflammatory functions of STAT3

Blood ◽  
2014 ◽  
Vol 123 (19) ◽  
pp. 2978-2987 ◽  
Author(s):  
Scott M. Steward-Tharp ◽  
Arian Laurence ◽  
Yuka Kanno ◽  
Alex Kotlyar ◽  
Alejandro V. Villarino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Mouse Model ◽  
Bacterial Infection ◽  
Marrow Transplantation ◽  
Key Points ◽  
Anti Inflammatory

Key Points Mice that express a mutation in STAT3 phenocopy patients with HIES. Bone marrow transplantation does not fully correct the susceptibility of these animals to bacterial infection.

Effect of mononuclear phagocyte system deficiency and of yeast polysaccharide injections on heterotopic bone marrow formation

1988 ◽  
Vol 106 (1) ◽  
pp. 1026-1028
Author(s):  
S. V. Gurina ◽  
I. N. Ivasenko ◽  
M. A. Kashkina ◽  
A. Yu. Zaritskii ◽  
G. E. Arkad'eva
Keyword(s):  
Bone Marrow ◽  
Mononuclear Phagocyte ◽  
Mononuclear Phagocyte System ◽  
Heterotopic Bone

scholarly journals A transgenic line that reports CSF1R protein expression provides a definitive marker for the mouse mononuclear phagocyte system

2020 ◽  
Author(s):  
Kathleen Grabert ◽  
Anuj Sehgal ◽  
Katharine M. Irvine ◽  
Evi Wollscheid-Lengeling ◽  
Derya D. Ozdemir ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Mononuclear Phagocyte ◽  
Mononuclear Phagocyte System ◽  
Lymphoid Tissues ◽  
Direct Role ◽  
Hematopoietic Stem ◽  
C Terminus ◽  
Monocytes And Macrophages ◽  
Macrophage Populations

AbstractThe proliferation, differentiation and survival of cells of the mononuclear phagocyte system (MPS, progenitors, monocytes, macrophages and classical dendritic cells) is controlled by signals from the macrophage colony-stimulating factor receptor (CSF1R). Cells of the MPS lineage have been identified using numerous surface markers and transgenic reporters but none is both universal and lineage-restricted. Here we report the development and characterization of a novel CSF1R reporter mouse. A Fusion Red (FRed) cassette was inserted in-frame with the C-terminus of CSF1R, separated by a T2A-cleavable linker. The insertion had no effect of CSF1R expression or function. CSF1R-FRed was expressed in monocytes and macrophages and absent from granulocytes and lymphocytes. In bone marrow, CSF1R-FRed was absent in lineage-negative hematopoietic stem cells (HSC), arguing against a direct role for CSF1R in myeloid lineage commitment. It was highly-expressed in marrow monocytes and common myeloid progenitors (CMP) but significantly lower in granulocyte-macrophage progenitors (GMP). In sections of bone marrow, CSF1R-FRed was also detected in osteoclasts, CD169+ resident macrophages and, consistent with previous mRNA analysis, in megakaryocytes. In lymphoid tissues, CSF1R-FRed highlighted diverse MPS populations including classical dendritic cells. Whole mount imaging of non-lymphoid tissues in mice with combined CSF1R-FRed/Csf1r-EGFP confirmed the restriction of CSF1R expression to MPS cells. The two markers highlight the remarkable abundance and regular distribution of tissue MPS cells including novel macrophage populations within tendon and skeletal muscle and underlying the mesothelial/serosal/capsular surfaces of every major organ. The CSF1R-FRed mouse provides a novel reporter with exquisite specificity for cells of the MPS.

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