scholarly journals Redox Profiling Reveals Clear Differences between Molecular Patterns of Wound Fluids from Acute and Chronic Wounds

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Edina Bodnár ◽  
Edina Bakondi ◽  
Katalin Kovács ◽  
Csaba Hegedűs ◽  
Petra Lakatos ◽  
...  
Keyword(s):  
Chronic Wounds ◽  
Chronic Wound ◽  
Skin Diseases ◽  
Cell Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leg Ulcers ◽  
Local Circulation ◽  
Compensatory Mechanisms ◽  
Parp Activation

Wound healing is a complex multiphase process which can be hampered by many factors including impaired local circulation, hypoxia, infection, malnutrition, immunosuppression, and metabolic dysregulation in diabetes. Redox dysregulation is a common feature of many skin diseases demonstrated by virtually all cell types in the skin with overproduction of reactive oxygen and nitrogen species. The objective of this study was to characterize the redox environment in wound fluids and sera from patients suffering from chronic leg ulcers (n=19) and acute wounds (bulla fluids from second degree burns; n=11) with serum data also compared to those from healthy volunteers (n=7). Significantly higher concentrations of TNF-α, interleukine-8, vascular endothelial growth factor, and lactate dehydrogenase (measure of cell damage) were found in fluids from chronic wounds compared to acute ones. The extent of protein carbonylation (measure of protein oxidation), lipid peroxidation, and tyrosine nitration (indicator of peroxynitrite production) was similar in acute and chronic wound fluids, while radical scavenging activity and glutathione (GSH) levels were elevated in chronic wound fluids compared to acute wounds. Sera were also assessed for the same set of parameters with no significant differences detected. Nitrotyrosine (the footprint of the potent oxidant peroxynitrite) and poly(ADP-ribose) (the product of the DNA damage sensor enzyme PARP-1) could be detected in wound biopsies. Our data identify multiple signs of redox stress in chronic wounds with notable differences. In chronic wounds, elevations in antioxidant levels/activities may indicate compensatory mechanisms against inflammation. The presence of nitrotyrosine and poly(ADP-ribose) in tissues from venous leg ulcers indicate peroxynitrite production and PARP activation in chronic wounds.

Biological Activities of Carlina Oxide Isolated from the Roots of Carthamus caeruleus

2020 ◽  
Vol 10 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Imane Rihab Mami ◽  
Rania Belabbes ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Penicillium Expansum ◽  
Natural Food ◽  
Antioxidant Power

Background: Carthamus caeruleus belongs to the Asteraceae family. The roots are traditionally used as healing agents. They help to heal burns and treat skin diseases. They are also used against joint inflammation and are very effective against diseases such as irritable bowel syndrome for cancer patients. Objectives: The purpose of this work was i) to study the chemical composition of i) the essential oil and hydrosol extract of Carthamus caeruleus, ii) to isolate the major component of both extracts and iii) to evaluate their antioxidant, antifungal and insecticidal activities. Methods: The essential oil and hydrosol extract obtained from the roots were studied by GC and GC/MS. The antioxidant activities were performed using two different methods i) Radical scavenging activity (DPPH) and ii) the Ferric-Reducing Antioxidant Power (FRAP), using BHT as a positive control. Whereas, the antifungal activity of the essential oil and Carlina oxide was investigated against plant fungi. The fumigation toxicity of C. caeruleus essential oil besides Carlina oxide was evaluated against adults of Bactrocera oleae better known as the olive fly. Results: The essential oil and hydrosol extract were mainly represented by acetylenic compounds such as carline oxide and 13-methoxy carline oxide. Carlina oxide was isolated and identified by 1H and 13C NMR spectroscopic means. The results showed that Carlina oxide presented interesting antioxidant and antifungal properties, while C. caeruleus root essential oil had better insecticidal activity. Furthermore, Carlina oxide has demonstrated promising in vivo antifungal activity to control infection of apples by Penicillium expansum. Conclusion: Carlina oxide can be used as a natural food preservative and alternative to chemical fungicides to protect stored apple against Penicillium expansum.

scholarly journals POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

2021 ◽  
Vol 45 ◽  
Author(s):  
Andréa Michel Sobottka ◽  
Elisandra Tessaro ◽  
Suelen Maier da Silva ◽  
Marina Pedron ◽  
Lara Tortini Seffrin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Butanol Fraction ◽  
Degraded Ecosystems

ABSTRACT Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

Chemical Composition of Essential Oil, Antioxidant, Antidiabetic, Anti-obesity, and Neuroprotective Properties of Prangos gaubae

2017 ◽  
Vol 12 (12) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Mir Babak Bahadori ◽  
Gokhan Zengin ◽  
Shahram Bahadori ◽  
Filippo Maggi ◽  
Leila Dinparast
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Skin Diseases ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Volatile Components ◽  
Total Antioxidant ◽  
Pharmaceutical Industries

Chemical composition of essential oil and the potential of Prangos gaubae for the management of public health problems such as Alzheimer's disease, obesity, Diabetes mellitus, and skin diseases were evaluated for the first time. In this direction, enzyme inhibitory effects, antioxidant activity, and total bioactive contents of the plant were determined. EO showed high acetylcholinesterase (2.97 mg GEs/g oil), α-amylase (1.35 mmol ACEs/g oil), α-glucosidase (38.84 mmol ACEs/g oil), and lipase (1.59 mmol OEs/g oil) inhibitory activities. Moreover, strong antioxidant effects were observed in antiradical (DPPH and ABTS), reducing power (CUPRAC and FRAP), total antioxidant, and metal chelating assays. Methanol extract exhibited promising DPPH radical scavenging activity (0.47 mmol TEs/g extract) and also high reducing power in CUPRAC (0.89 mmol TEs/g extract) and FRAP (0.52 mmol TEs/g extract) assays. All extracts showed low total flavonoid but high total phenolics content. Furthermore, they exhibited strong skin-care effect in tyrosinase inhibition assay. EO analysis showed the presence of germacrene D (26.7%), caryophyllene oxide (14.3%), ( E)-caryophyllene (13.8%), and spathulenol (11.3%) as the major volatile components. Results indicated that P. gaubae has promising potential for possible uses in food, cosmetic, and pharmaceutical industries due to its valuable phytoconstituents and biological activities.

scholarly journals Effect of Chitosan Nanoparticle-Loaded Thymus serpyllum on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Salma Baig ◽  
Ainnul Hamidah Syahadah Azizan ◽  
Hanumantha Rao Balaji Raghavendran ◽  
Elango Natarajan ◽  
Sangeetha Naveen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Cell Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Potential Candidate ◽  
Protective Effects ◽  
Active Components ◽  
Thymus Serpyllum

We have determined the protective effects of Thymus serpyllum (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) in vitro. Gas chromatography–mass spectroscopy confirmed the spectrum of active components in the extract. Out of the three different extracts, the hexane extract showed significant free radical scavenging activity. Treatment of MSCs with H2O2 (hydrogen peroxide) significantly increased intracellular cell death; however, pretreatment with TS extract and nanoparticle-loaded TS (200 μg/ml) suppressed H2O2-induced elevation of Cyt-c and MMP13 and increased the survival rates of MSCs. H2O2-induced (0.1 mM) changes in cytokines were attenuated in the extract and nanoparticles by pretreatment and cotreatment at two time points (p<0.05). H2O2 increased cell apoptosis. In contrast, treatment with nanoparticle-loaded TS suppressed the percentage of apoptosis considerably (p<0.05). Therefore, TS may be considered as a potential candidate for enhancing the effectiveness of MSC transplantation in cell therapy.

scholarly journals Lanostane Triterpenoid Metabolites from a Penares sp. Marine Sponge Protect Neuro-2a Cells against Paraquat Neurotoxicity

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5397
Author(s):  
Ekaterina A. Yurchenko ◽  
Sophia A. Kolesnikova ◽  
Ekaterina G. Lyakhova ◽  
Ekaterina S. Menchinskaya ◽  
Evgeny A. Pislyagin ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Cell Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Neuroprotective Activity ◽  
Hsp70 Expression ◽  
Protective Effects ◽  
Intracellular Level ◽  
Intracellular Ros ◽  
Lanostane Triterpenoid

The results of an investigation of the protective effects of five lanostane triterpenoids: 3β-acetoxy-7β,8β-epoxy-5α-lanost-24-en-30,9α-olide (1), 3β-hydroxy-7β,8β-epoxy-5α-lanost-24-en- 30,9α-olide (2), 29-nor-penasterone (3), penasterone (4), and acetylpenasterol (5), from a marine sponge, Penares sp., against paraquat-induced neuroblastoma Neuro-2a cell damage, are described. The influence of all compounds on viability of the Neuro-2a cells treated with paraquat (PQ) was studied with MTT and fluorescein diacetate assays as well as propidium iodide straining. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the compounds as well as their influence on reactive oxygen species (ROS) level and mitochondrial membrane potential in PQ-treated neuronal cells were analyzed. Finally, the effect of the compounds on intracellular level of heat shock protein 70 kDa (Hsp70) and neurite outgrowth in PQ-treated Neuro-2a cells were studied. Studied triterpenoids demonstrated protective effects against PQ-induced neurotoxicity associated with the ability to reduce ROS intracellular level and diminish mitochondrial dysfunction. Acetylpenasterol (5), as a more promising neuroprotective compound, significantly increased the viability of Neuro-2a cells incubated with PQ as well as decreased intracellular ROS level in these cells. Moreover, acetylpenasterol induced Hsp70 expression in PQ-treated cells. It was also shown to inhibit PQ-induced neurite loss and recovered the number of neurite-bearing cells. The relationship between neuroprotective activity of the investigated compounds 1–5 and their chemical structure was also discussed.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND WOUND HEALING ACTIVITY OF WHEATGRASS BY 1,1- DIPHENYL, 2 PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (12) ◽  
pp. 170
Author(s):  
Veermaneni Alekhya ◽  
Thiyagarajan Deepan ◽  
Magharla Dasaratha Dhanaraju
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Wheat Plant ◽  
Wide Range ◽  
Wound Healing Activity ◽  
In Vitro Antioxidant Activity

Objective: This study was designed to evaluate in vitro antioxidant activity and wound healing activity in Triticum aestivum (wheat grass).Methods: T. aestivum commonly known as Wheatgrass had a wide range of health benefits among the young grass of common wheat plant components includes chlorophyll, flavonoids, and Vitamins A, C, and E. Wheatgrass is used in Folklore medicine for treatment of skin diseases and wound healing. In our present study, petroleum ether, ethanol and aqueous extracts of T. aestivum have been evaluated for in vitro antioxidant activity and wound healing activity by 1,1- diphenyl, 2 Picrylhydrazyl radical scavenging activity, and Chick chorioallantoic method, respectively.Results: The results of both the assay showed that all the extracts of T. aestivum have significant antioxidant and wound healing activity on dose-dependent manner.Conclusion: The wheatgrass has antioxidant and wound healing activity.

scholarly journals QUALITATIVE AND QUANTITATIVE PHYTOCHEMICALANALYSIS AND DPPH RADICAL SCAVENGING ACTIVITY OF DIFFERENT EXTRACTSOF FLOWER OF BAUHINIA ACUMINATA LINN

2017 ◽  
Vol 9 (6) ◽  
pp. 23
Author(s):  
Sreeja Sanjeev ◽  
Regi Raphael K.
Keyword(s):  
Secondary Metabolites ◽  
Skin Diseases ◽  
Quantitative Estimation ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Primary And Secondary Metabolites ◽  
Qualitative And Quantitative

Objective: Bauhinia acuminate L (Fabaceae) is a species of flowering shrub native to tropical south eastern Asia. The flowerof B. acuminataare used against various skin diseases,worms, tumours and diabetes. The Indian vaiydas recommended as a remedy to treat severe biliousness.The qualitative and quantitative estimation of phytochemical constituents in various extracts and the antioxidant potential of flower of Bauhinia acuminata were evaluated.Methods: All the analysis was done based on standard protocols.Results: The phytochemical screening reveals the presence of various primary and secondary metabolites like sugar, carbohydrate, amino acid, fat, quinone, steroids, phenol, saponin, alkaloids, and acid content. In quantitative analysis the important secondary metabolites such as alkaloids and phenolic compounds were estimated in all the extracts.The ethanol and chloroformextract produced significant antioxidant properties in a dose-dependent manner. DPPH free radical scavenging assay of ethanol and chloroform extracts of flower exhibited IC 50 values of 24.44±1.201μg/ml and 196.68±0.456μg/ml respectively. At 1000 μg/ml concentration both extracts shows maximum radical scavenging activity (98.97% and 85.67 %).Conclusion: The findings of this study indicate that this plant is medicinal with prominent antioxidant property.

scholarly journals A mechanistic approach to anti-nociceptive potential of Nymphaea lotus Linn (Nymphaeaceae) in rodents

2020 ◽  
Vol 9 (5) ◽  
pp. 291-299
Author(s):  
E.O Agbaje ◽  
◽  
A Iwe ◽  
OJ Oguntokun ◽  
◽  
...  
Keyword(s):  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
K Channel ◽  
Total Phenolic ◽  
Hot Plate ◽  
Adrenergic Systems ◽  
Tail Clip

Background: Nymphaea lotus Linn. (Nymphaeaceae), commonly known as white water-lily, white lotus or Egyptian lotus, is an important and well-known medicinal plant, widely used in the Ayurveda and Siddha systems of medicine for the treatment of diabetes, inflammation, liver disorders, urinary disorders, fever, skin diseases, cancer, gonorrhoea, pain and bronchitis. Objective: The study was designed to explore anti-nociceptive potential of aqueous extract of Nymphaea lotus leaf, its possible mechanism of action, and antioxidant properties. Methods: The anti-nociceptive activity of Nymphaea lotus (50, 100 and 250 mg/kg) was explored using writhing, formalin, tail clip and hot plate tests, while formalin test was used to investigate the involvement of opioid, dopamine, serotonin, K+ channel blocker, α1-adrenergic and α2- adrenergic systems. The antioxidant effect was carried out using DPPH, nitric oxide free radical scavenging activity and the reducing power effect. Total phenolic and flavonoids contents were also explored. Results: Oral administration of N. lotus in doses of 50, 100 and 250 mg/kg recorded a significant (p<0.05) dose dependent obstruction of nociception. A remarkable effect was recorded with the writhing and formalin tests and a significant effect was also observed in the tail clip and hot plate test, which suggests peripheral and central anti-nociceptive activity of the extract. The anti-nociceptive effect produced by N. lotus was significantly reversed by naloxone and yohimbine, suggesting the possible involvement of opioid and α2–adrenergic systems in its anti-nociceptive activity. N. lotus also displayed a potent antioxidant activity. Conclusion: These findings justify the folkloric use of N. lotus in pain management.

scholarly journals The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Hiromi Murase ◽  
Masamitsu Shimazawa ◽  
Mamoru Kakino ◽  
Kenji Ichihara ◽  
Kazuhiro Tsuruma ◽  
...  
Keyword(s):  
Cell Viability ◽  
White Light ◽  
Cell Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Tetrazolium Salt ◽  
Fibroblast Cells ◽  
Skin Damage ◽  
Uva Irradiation ◽  
Brazilian Green Propolis

Background. We investigated the effects of Brazilian green propolis and its constituents against white light- or UVA-induced cell damage in mouse retinal cone-cell line 661W or human skin-derived fibroblast cells (NB1-RGB).Methods. Cell damage was induced by 3,000lx white light for 24 h or 4/10 J/cm2UVA exposure. Cell viability was assessed by Hoechst33342 and propidium iodide staining or by tetrazolium salt (WST-8) cell viability assay. The radical scavenging activity of propolis induced by UVA irradiation in NB1-RGB cells was measured using a reactive-oxygen-species- (ROS-) sensitive probe CM-H2DCFDA. Moreover, the effects of propolis on the UVA-induced activation of p38 and extracellular signal-regulated kinase (ERK) were examined by immunoblotting.Results. Treatment with propolis and two dicaffeoylquinic acids significantly inhibited the decrease in cell viability induced by white light in 661W. Propolis and its constituents inhibited the decrease in cell viability induced by UVA in NB1-RGB. Moreover, propolis suppressed the intracellular ROS production by UVA irradiation. Propolis also inhibited the levels of phosphorylated-p38 and ERK by UVA irradiation.Conclusion. Brazilian green propolis may become a major therapeutic candidate for the treatment of AMD and skin damage induced by UV irradiation.

Formulation and evaluation of polyherbal cream and lotion to treat psoriasis-induced secondary infections

2019 ◽  
Vol 14 ◽  
Author(s):  
Priyanka Joshi ◽  
Sushil Joshi ◽  
Urvashi Rajani ◽  
Ruchi Badoni Semwal ◽  
Deepak Kumar Semwal
Keyword(s):  
Essential Oils ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Ms Analysis ◽  
Secondary Infections ◽  
Scalp Psoriasis ◽  
Agar Media ◽  
Dpph Radical Scavenging

Background: Psoriasis is one of the most common skin diseases in human and affected a major population worldwide. Aim: The aim of the present study is to evaluate the efficacy of selected polyherbal formulations against psoriasis-induced secondary infections. Methods: Samples were collected from the scalp, behind the ears, chest and back of the patients. The microscopic examination of fungal and bacterial growth was carried out with lactophenol cotton blue stain and gram stain respectively. Volatile constituents of essential oils were identified by GC-MS analysis, in order to investigate the relation between chemical composition and biological activity. Nutrient agar media was used for antibacterial activity whereas Sabourauds dextrose agar media was used for antifungal activity. Results: A total of 24 isolates were obtained from 2 patient of scalp psoriasis. Anti-oxidant activity of the samples was determined using DPPH radical scavenging method. In the present study, EOs showed a good antifungal action against Malassezia pachydermatis and other microbial strains. The GC-MS analysis revealed the presence of eugenol, linalool, citral, neral, limonene, terpenes, eucalyptol and thymol in the essential oils. The formulated retention lotion 1 (L4), 2 (L5), 3 (L6) and cream 1 (C1) showed DPPH radical scavenging activity by 23.52%, 24.48%, 28% and 5.08%, respectively. Conclusion: The present study concluded that most of the formulated lotions and creams showed good antimicrobial activity and may be applied topically against scalp psoriasis.

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