scholarly journals Removal of Nitrate in Simulated Water at Low Temperature by a Novel Psychrotrophic and Aerobic Bacterium, Pseudomonas taiwanensis Strain J

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Tengxia He ◽  
Qing Ye ◽  
Quan Sun ◽  
Xi Cai ◽  
Jiupai Ni ◽  
...  
Keyword(s):  
Low Temperature ◽  
Carbon Source ◽  
Phospholipid Fatty Acid ◽  
Nitrate Removal ◽  
Aerobic Denitrification ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
High Ph ◽  
Removal Efficiencies ◽  
Psychrotrophic Strain

Low temperatures and high pH generally inhibit the biodenitrification. Thus, it is important to explore the psychrotrophic and alkali-resisting microorganism for degradation of nitrogen. This research was mainly focused on the identification of a psychrotrophic strain and preliminary explored its denitrification characteristics. The new strain J was isolated using the bromothymol blue solid medium and identified as Pseudomonas taiwanensis on the basis of morphology and phospholipid fatty acid as well as 16S rRNA gene sequence analyses, which is further testified to work efficiently for removing nitrate from wastewater at low temperature circumstances. This is the first report that Pseudomonas taiwanensis possessed excellent tolerance to low temperature, with 15°C as its optimum and 5°C as viable. The Pseudomonas taiwanensis showed unusual ability of aerobic denitrification with the nitrate removal efficiencies of 100% at 15°C and 51.61% at 5°C. Single factor experiments showed that the optimal conditions for denitrification were glucose as carbon source, 15°C, shaking speed 150 r/min, C/N 15, pH≥7, and incubation quantity 2.0 × 106 CFU/mL. The nitrate and total nitrogen removal efficiencies were up to 100% and 93.79% at 15°C when glucose is served as carbon source. These results suggested that strain J had aerobic denitrification ability, as well as the notable ability to tolerate the low temperature and high pH.

Remediation of nitrate-contaminated wastewater using denitrification biofilters with straws of ornamental flowers added as carbon source

2016 ◽  
Vol 74 (2) ◽  
pp. 416-423 ◽  
Author(s):  
Junjun Chang ◽  
Luyao Ma ◽  
Yuanyang Zhou ◽  
Shenghua Zhang ◽  
Weilu Wang
Keyword(s):  
Carbon Source ◽  
Significant Positive Correlation ◽  
Inorganic Carbon ◽  
Nitrate Removal ◽  
Temporal Variations ◽  
Phosphate Removal ◽  
Aerobic Degradation ◽  
Organic Substances ◽  
Batch Mode ◽  
Removal Efficiencies

Straws of four ornamental flowers (carnation, rose, lily, and violet) were added into denitrification biofilters using gravel as matrix through vertically installed perforated polyvinylchloride pipes to provide organic carbon for the treatment of nitrate-contaminated wastewater operating in batch mode. Removal efficiencies of nitrate and phosphate, as well as temporal variations of nitrogen and carbon during batches 10 and 19, were investigated and assessed. Nitrate removal was efficiently enhanced by the addition of flower straws, but decreased gradually as the organic substances were consumed. Phosphate removal was also improved, although this very limited. High nitrate removal rates were achieved during the initial 12 h in the two batches each lasting for 3 days, along with the depletion of influent dissolved oxygen due to aerobic degradation of the organic compounds. NO2−-N of 0.01–2.83 mg/L and NH4+-N of 0.02–1.69 mg/L were formed and both positively correlated to the nitrate reduced. Inorganic carbon (IC) concentrations increased during the batches and varied conversely with the nitrate contents, and could be indicative of nitrate removal due to the highly significant positive correlation between NO3−-N removed and IC concentration (r2 = 0.881, p < 0.0001). It is feasible and economical to use the denitrification biofilter to treat nitrate-contaminated wastewater, although further optimization of carbon source addition is still required.

Nitrate Removal of Fluidized-Bed Biofilm Reactors Using Starch / Polyvinyl Alcohol Blends as Carbon Source and Carrier Simultaneously at Low Temperature

2014 ◽  
Vol 501-504 ◽  
pp. 2089-2092
Author(s):  
Hai Hong Zhou ◽  
Fang He
Keyword(s):  
Drinking Water ◽  
Controlled Release ◽  
Low Temperature ◽  
Carbon Source ◽  
Polyvinyl Alcohol ◽  
Fluidized Bed ◽  
Nitrate Concentration ◽  
Nitrate Removal ◽  
Biofilm Reactors ◽  
Resident Time

A kind of controlled-release carbon source, starch / polyvinyl alcohol blends (SPVA), was used as both carbon source and biofilm supporter in laboratory-scale fluidized-bed biofilm reactors (FBBRs) to remove nitrate from groundwater. Results show: when the influent nitrate concentration was 100 mg-N /L, FBBRs packed with SPVA can effectively remove nitrate from groundwater at the condition of temperature 20 °C, hydraulic resident time (HRT) 4 h. The effluent nitrate can meet with the Chinese drinking water standards at low temperature (15-2 °C) by adjusted the HRT of FBBRs. The denitrification rate declined nonlinearly with the decrease of temperature and changed sharply in the range of 20-15 °Cand 10-5 °C.

Comparison of aerobic denitrifying activity among three cultural species with various carbon sources

2004 ◽  
Vol 50 (8) ◽  
pp. 15-22 ◽  
Author(s):  
Y. Otani ◽  
K. Hasegawa ◽  
K. Hanaki
Keyword(s):  
Carbon Source ◽  
Nitrate Reduction ◽  
Sodium Acetate ◽  
Nitrate Removal ◽  
Carbon Sources ◽  
Alcaligenes Faecalis ◽  
Aerobic Denitrification ◽  
Denitrification Activity ◽  
Paracoccus Pantotrophus ◽  
Nitrate And Nitrite

Abilities of three aerobic denitrifiers such as Alcaligenes faecalis, Microvirgula aerodenitrificans and Paracoccus pantotrophus were compared from the viewpoints of nitrate removal efficiency and organic matter utilization. First, the effect of carbon source was investigated. Although nitrate reduction was observed in all strains under aerobic conditions, a change of carbon source considerably affected the denitrification ability. In the case of P. pantotrophus, nitrate and nitrite were completely removed in three days under sodium acetate or leucine as a carbon source. In the case of A. faecalis, sufficient nitrate removal was observed only when sodium acetate or ethanol was added. P. pantotrophus and A. faecalis showed a higher ability of nitrate removal than that of M. aerodenitrificans. Therefore, P. pantotrophus was selected in order to investigate the effects of concentration and repetitive addition of carbon. Sodium acetate was used as a sole carbon source. Nitrate was not reduced when the carbon concentration was below 500 mgC/L. However, when carbon source was added repeatedly, nitrate was reduced under 100 mgC/L after the optical density of the bacterium reached above 1.0. This result indicated that a high enough level of bacterial density was necessary to express aerobic denitrification activity.

Denitrification characteristics of a newly isolated indigenous aerobic denitrifying bacterium under oligotrophic conditions

2015 ◽  
Vol 72 (7) ◽  
pp. 1082-1088 ◽  
Author(s):  
Jun-feng Su ◽  
Kai Zhang ◽  
Ting-lin Huang ◽  
Fang Ma ◽  
Lin Guo ◽  
...  
Keyword(s):  
Nitrate Removal ◽  
Reduction Rate ◽  
Inoculum Size ◽  
Aerobic Denitrification ◽  
Rrna Gene ◽  
Box Behnken Design ◽  
Initial Ph ◽  
Bacterium Strain ◽  
Nitrate Degradation ◽  
Full Factorial

A novel indigenous bacterium, strain JM10, isolated from the oligotrophic Hei He reservoir was characterized and showed aerobic denitrification ability. JM10 was identified as Bacillus sp. by phylogenetic analysis of its 16S rRNA gene sequence. Strain JM10 displayed very high levels of activity in aerobic conditions, consuming over 94.3% NO3−-N (approximately 3.06 mg L−1) with a maximum reduction rate of 0.108 mg NO3−-N L−1 h−1. Full-factorial Box–Behnken design and response surface methodology were employed to investigate the optimal nitrate degradation conditions. The optimum conditions for nitrate degradation, at a rate of 0.140 mg L−1 h−1, were found to be an inoculum size of 16.3% v/v, initial pH of 7.6, C/N ratio of 7.4, and temperature of 27.4 °C, and the C/N ratio and temperature had the largest effect on the nitrate degradation rate. Strain JM10 was added into the water samples from Hei He reservoir and the total nitrogen and nitrate removal rates of the strain reached 66.5% and 100%, respectively. Therefore, our results demonstrate that the strain JM10 favored the bioremediation of the oligotrophic reservoir.

scholarly journals Biochar enhanced the nitrogen removal performance of heterotrophic nitrification-aerobic denitrification strain pseudomonas fluorescent sp. Z03 for insufficient carbon source wastewater under low temperature

2021 ◽  
Author(s):  
Jie Jiang ◽  
XiaoyanXu ◽  
Zhina Guo ◽  
Lianglun Sun ◽  
Meizhen Tang
Keyword(s):  
Electron Transfer ◽  
Low Temperature ◽  
Carbon Source ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Redox Activity ◽  
Aerobic Denitrification ◽  
Control Group ◽  
N Removal

Abstract In this study, biochar BC400 and BC700 were prepared, characterized and coupled with heterotrophic nitrification-aerobic denitrification (HNAD) strain Z03 for nitrogen removal experiments. The characterization results showed that BC700 has a higher specific surface area and a more complex multilayered pore structure, with increased aromatic condensation and higher crystallinity. BC400 and BC700 both have good redox activity, while BC400 has stronger electron donor capacities and BC700 owns better electron transfer properties. In addition, both BC400 and BC700 contain relatively high levels of dissolved organic carbon (DOC), reaching at 62.95 and 51.617mg/g respectively. BC400/BC700 coupled with strain Z03 can significantly improve the NH4+-N removal performance of low-temperature and low C/N wastewater compared with the control group. At a dosage of 4.0 g/L, the removal rate of NH4+-N reached to 95.16% (BC400 + Z03) and 84.37% (BC700 + Z03) within 72h, respectively. Higher than the sum of adsorption by BC400/BC700 (16.19%/18.85%) and microbial degradation (41.03%). Besides, the BC400 + BC700 + Z03 NH4+-N removal systems provide higher nitrogen removal efficiencies than BC400/BC700 + Z03 nitrogen removal systems. When the dosage (BC400 + BC700, mass ratio 5:1) reaches 3.0g/L, it can achieve more than 90% NH4+-N removal rate within 48h. The reasons for the promotion of biochar on microbial denitrification were analyzed as follows: 1) DOC can provide an additional carbon source for microorganisms; 2) biochar, as a pH buffer, can neutralize the acidity due to nitrification; 3) BC400 and BC700, as materials with good redox activity, may play a role in promoting the activity of electron transfer system and enzyme activity.

scholarly journals Oceanobacillus arenosus sp. nov., a moderately halophilic bacterium isolated from marine sand

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2943-2948 ◽  
Author(s):  
Wonyong Kim ◽  
Chatuphon Siamphan ◽  
Jong-Hwa Kim ◽  
Ampaitip Sukhoom
Keyword(s):  
Halophilic Bacterium ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Marine Sand ◽  
Moderately Halophilic Bacterium ◽  
Distinct Lineage ◽  
Lipid Pattern

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterium, designated CAU 1183T, was isolated from marine sand and its taxonomic position was investigated by using a polyphasic approach. The bacterium grew optimally at 30 °C, at pH 8.5 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1183T formed a distinct lineage within the genus Oceanobacillus and exhibited the highest similarity to Oceanobacillus chungangensis CAU 1051T (97.6 %). The strain contained MK-7 as the predominant isoprenoid quinone and anteiso-C15 : 0 was the major cellular fatty acid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipid pattern of strain CAU 1183T consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified lipids, including two phospholipids, two glycolipids, a phosphoglycolipid and two lipids. The G+C content of the genomic DNA was 37.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CAU 1183T should be assigned to a novel species in the genus Oceanobacillus, for which the name Oceanobacillus arenosus sp. nov. is proposed. The type strain is CAU 1183T ( = KCTC 33037T = CECT 8560T).

Aggregatimonas Sangjinii Gen. Nov., Sp. Nov., A Novel Silver Nanoparticle Synthesizing Bacterium Belonging to the Family Flavobacteriaceaee

2021 ◽  
Author(s):  
Dawoon Chung ◽  
Jaoon Young Hwan Kim ◽  
Kyung Woo Kim ◽  
Yong Min Kwon
Keyword(s):  
16S Rrna ◽  
Genome Sequence ◽  
Sequence Similarity ◽  
Whole Genome Sequence ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
16S Rrna Sequence ◽  
Respiratory Quinone ◽  
The Family ◽  
The Media

Abstract A gram-negative, orange-pigmented, non-flagellated, gliding, rod-shaped, and aerobic bacterium, designated strain F202Z8T, was isolated from a rusty iron plate found in the intertidal region of Taean, South Korea. Notably, this strain synthesized silver nanoparticles (AgNPs), and 17 putative genes responsible for the synthesis of AgNPs were found in its genome. The complete genome sequence of strain F202Z8T is 4,723,614 bp, with 43.26% G + C content. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain F202Z8T forms a distinct lineage with closely related genera Maribacter, Pelagihabitans, Pseudozobellia, Zobellia, Pricia, and Costertonia belonging to the family Flavobacteriaceae. The 16S rRNA sequence similarity was < 94.5%. The digital DNA–DNA hybridization and average nucleotide identity values calculated from the whole genome-sequence comparison between strain F202Z8T and other members of the family Flavobacteriaceae were in the ranges of 12.7–16.9% and 70.3–74.4%, respectively. Growth was observed at 15–33°C (optimally at 30°C), at pH 6.5–7.5 (optimally at pH 7.0), and with the addition of 2.5–4.5% (w/v) NaCl to the media (optimally at 4.0%). The predominant cellular fatty acids were iso-C15: 0, iso-C15 :1 G, and iso-C17 :0 3-OH; the major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine, five unidentified lipids, and two unidentified aminolipids. Our polyphasic taxonomic results suggested that this strain represents a novel species of a novel genus in the family Flavobacteriaceae, for which the name Aggregatimonas sangjinii gen. nov., sp. nov. is proposed. The type strain of Aggregatimonas sangjinii is F202Z8T (= KCCM 43411T = LMG 31494T).

Aquabacterium soli sp. nov., a novel bacterium isolated from soil under the long-term application of bifenthrin

2021 ◽  
Vol 71 (9) ◽  
Author(s):  
Lina Sun ◽  
Wei Chen ◽  
Kaihua Huang ◽  
Weiguang Lyu ◽  
Xinhua Gao
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Novel Bacterium ◽  
Pr China

Strain SJQ9T, an aerobic bacterium isolated from a soil sample collected in Shanghai, PR China, was characterized using a polyphasic approach. It grew optimally at pH 7.0, 30–35 °C and in the presence of 1 % (w/v) NaCl. A comparative analysis of 16S rRNA gene sequences showed that strain SJQ9T fell within the genus Aquabacterium . The closest phylogenetic relatives of strain SJQ9T were Aquabacterium citratiphilum DSM 11900T (98.6 % sequence similarity) and Aquabacterium commune DSM 11901T (96.4 %). Cells of the strain were Gram-stain-negative, motile, non-spore-forming, rod-shaped and positive for oxidase activity and negative for catalase. The chemotaxonomic properties of strain SJQ9T were consistent with those of the genus Aquabacterium : the major fatty acid was summed feature 3 (C16 : 1  ω6c and/or C16 : 1  ω7c). The isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 65.7 mol%. Strain SH9T exhibited a DNA–DNA relatedness level of 34±2 % with A. citratiphilum DSM 11900T and 28±3 % with A. commune DSM 11901T. Based on the obtained data, strain SJQ9T represents a novel species of the genus Aquabacterium , for which the name Aquabacterium soli sp. nov. is proposed. The type strain is SJQ9T (=JCM 33106T=CCTCC AB 2018284T).

Sign in / Sign up

Export Citation Format

Share Document