scholarly journals Guava Leaf Extract Diminishes Hyperglycemia and Oxidative Stress, Prevents β-Cell Death, Inhibits Inflammation, and Regulates NF-kB Signaling Pathway in STZ Induced Diabetic Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Muthukumaran Jayachandran ◽  
Ramachandran Vinayagam ◽  
Ranga Rao Ambati ◽  
Baojun Xu ◽  
Stephen Sum Man Chung
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Leaf Extract ◽  
Diabetes Management ◽  
Diabetic Rats ◽  
Lipid Peroxidation Product ◽  
Diabetes In Animals ◽  
Standard Drug ◽  
Medical Practitioners ◽  
Guava Leaf

Traditional Chinese medication has been utilized by Chinese medical practitioners to treat the varied symptoms of diabetes mellitus (DM). Notably, guava leaf has been used to treat diabetes in Asia. Our present study has been designed to analyze the action of guava leaf extract (GLE) at the molecular level in treating DM. A low dose of streptozotocin (STZ) was used to induce experimental diabetes in animals. Rats were treated with GLE at different concentrations (100, 200, and 400 mg/kg b.w.). The standard drug glibenclamide (GB) (600 μg/kg b.w.) was used for comparison. The diabetic rats showed a reduced level of insulin, accompanied by exaggerated levels of blood glucose, lipid peroxidation product, and augmented expressions of inflammatory cytokines, and showed reduced levels of antioxidants compared to the control rats. Supplementation with GLE counteracted the consequences of STZ. It suppresses the oxidative stress and inhibits the state of inflammation and the results are almost similar to that of standard drug group (GB group 5). Our present research, therefore, provides useful data concerning guava leaf extract by a thorough assessment in diabetes management. Being a natural product, additional analysis on GLE can shed light on finding effective phytochemicals within the field of diabetes mellitus.

Extract of Moringa concanensis Nimmo leaves ameliorates hyperglycemia and oxidative stress, and improves β-cell function in Alloxan monohydrate induced diabetic rat

2021 ◽  
Vol 17 ◽  
Author(s):  
Amerendra Singh ◽  
Jai Narayan Mishra ◽  
Santosh Kumar Singh ◽  
Vishal Kumar Vishwakarma ◽  
Shravan Kumar Paswan
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Diabetes Management ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Β Cells ◽  
Standard Drug ◽  
Alloxan Monohydrate ◽  
And Oxidative Stress

Background: The ethanomedicinal importance of Moringa concanensis Nimmo plant is reflected in Ayurvedic and traditional system of medicine. It brings out its importance as diverse plant in Ayurvedic preparation and diabetes management. Aims of study: The research was centred to bring out the Hyperglycemiccapabilities of Moringa concanensis Nimmo leaves Ethanolic extract (PE) on Alloxan monohydrate (AXM) induceddiabetic rat model. Materials and Methods: Wistar rats were made diabetic by AXM and treated with PE (200 mg/kg body weight) and glibenclamide as a standard drug. All Essential parameters like Fasting blood glucose (FBS), Post prandial blood glucose (PPBS), AST, ALT, ALP, ACP, LDH and oxidative stress markers were measured. Also to see β-cells structures histology of pancreas was also done. Results: The non toxicity of PE dose was confirmed by acute toxicity study and also this study models helped to know about the anti-hyperglycemic effects of PE by decreasing FBS and PPBS levels in the diabetic rats. It also enhances oxidative stress by decreasing MDA levels and elevating the GSH and SOD. The histopathological analysis helped us to know about structure decay of β-cells of pancreas tissue of diabetic rats. PEpotential was confirmed by serum enzymes AST, ALT, ALP, ACP and LDH as it showed significant decrease in diabetic rats. Conclusion: It was confirmed from the data that PE is efficient in governance of diabetes and its control, so there is a need to work at molecular level to bring out all its potential for the benefit of the society.

scholarly journals Yacon (Smallanthus sonchifolius) Leaf Extract Attenuates Hyperglycemia and Skeletal Muscle Oxidative Stress and Inflammation in Diabetic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Klinsmann Carolo dos Santos ◽  
Bianca Guerra Bueno ◽  
Luana Ferreira Pereira ◽  
Fabiane Valentini Francisqueti ◽  
Mariana Gobbo Braz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
The Other ◽  
Inflammatory Biomarkers ◽  
Smallanthus Sonchifolius ◽  
Hydroethanolic Extract

The effects of hydroethanolic extract of Yacon leaves (HEYL) on antioxidant, glycemic, and inflammatory biomarkers were tested in diabetic rats. Outcome parameters included glucose, insulin, interleukin-6 (IL-6), and hydrophilic antioxidant capacity (HAC) in serum and IL-6, HAC, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in soleus. The rats (10/group) were divided as follows: C, controls; C + Y, HEYL treated; DM, diabetic controls; and DM + Y, diabetic rats treated with HEYL. Diabetes mellitus was induced by administration of streptozotocin. C + Y and DM + Y groups received 100 mg/kg HEYL daily via gavage for 30 d. Hyperglycemia was improved in the DM + Y versus DM group. Insulin was reduced in DM versus C group. DM rats had higher IL-6 and MDA and lower HAC in the soleus muscle. HEYL treatment decreased IL-6 and MDA and increased HAC in DM rats. DM + Y rats had the highest CAT activity versus the other groups; GPx was higher in C + Y and DM + Y versus their respective controls. The apparent benefit of HEYL may be mediated via improving glucoregulation and ameliorating oxidative stress and inflammation, particularly in diabetic rats.

scholarly journals The Impact of Aqueous Leaf Extract of Soursop on Glucose and Lipid Profile in Alloxan Induced Diabetic Albino Rats

2021 ◽  
pp. 13-19
Author(s):  
P. E. Ejembi ◽  
J. I. Oche ◽  
J. O. Ejembi ◽  
L. S. Zaccheaus
Keyword(s):  
Lipid Profile ◽  
Leaf Extract ◽  
Diabetes Management ◽  
Diabetic Rats ◽  
Total Serum ◽  
Albino Rats ◽  
Total Serum Cholesterol ◽  
Standard Drug ◽  
Aqueous Leaf Extract ◽  
The Impact

The blood glucose, total serum cholesterol, High Density Lipoprotein (HDL) and Triglyceride (TRIG) levels of all rats in each group were determined before induction, post-induction with alloxan and post treatment with various concentrations of extract and standard drug. The alloxan diabetic rats treated with gliberclamide and aqueous leaf extract of Annona muricata showed average means of body weights as; (235.73±3.14a, 263.94±2.25a and 236.5±1.74a) respectively after 4 weeks of treatment. The Glucose level revealed; 168.43±5.06e, 65.29±4.57f and 57.86±3.93e respectively. Lipid profile raised significantly post exposure of diabetic rats to both standard drug and extract after 4 weeks of treatment. Therefore, A. muricata compared favorably with the standard drug in the context of diabetes management.

Barley microgreen incorporation in diet-controlled diabetes and counteracted aflatoxicosis in rats

2021 ◽  
pp. 153537022110597
Author(s):  
Sara M Mohamed ◽  
Emam A Abdel-Rahim ◽  
Tahany AA Aly ◽  
AbdelMoneim M Naguib ◽  
Marwa S Khattab
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetes Management ◽  
Cell Function ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Unhealthy Lifestyle

Increased environmental pollution and unhealthy lifestyle are blamed for escalated chronic diseases. Exposure to aflatoxins was recently suggested to have a role in the increased incidence of type 2 diabetes mellitus. Diet modification and consumption of different functional food are now gaining attention, especially in diabetes management. This study investigates the effect of a diet containing barley microgreen against diabetes induced by streptozotocin with or without aflatoxin administration in rats. Barley microgreen was rich in 3′-Benzyloxy-5,6,7,4′-tetramethoxyflavone (48.8% of total) followed by 5β,7βH,10α-Eudesm-11-en-1α-ol (18.46%). Streptozotocin injection and/or aflatoxin administration significantly elevated glucose level, decreased insulin level, decreased β-cell function, deteriorated liver and kidney function parameters, and induced oxidative stress in the liver. Histopathology revealed irregular small-sized islets and decreased area % of insulin-positive beta cells in the pancreas, hepatic degeneration, nephropathy, and neuropathy in diabetic and/or aflatoxin administered rats compared to control. Barley microgreen diet fed to diabetic rats with or without aflatoxin alleviated all evaluated parameters. Barley microgreen diet also ameliorated the toxic effect of aflatoxin. In conclusion, exposure to aflatoxin aggravated diabetes and its complication. The incorporation of barley microgreen in the diet was able to control type 2 diabetes mellitus and the improved outcomes observed with barley microgreen treatments involved or occurred in conjunction with improved biomarkers of oxidative stress.

Morus nigra leaf extract improves glycemic response and redox profile in the liver of diabetic rats

2015 ◽  
Vol 6 (11) ◽  
pp. 3490-3499 ◽  
Author(s):  
Carolina Morais Araujo ◽  
Karine de Pádua Lúcio ◽  
Marcelo Eustáquio Silva ◽  
Mauro César Isoldi ◽  
Gustavo Henrique Bianco de Souza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Metabolic Disorder ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Glycemic Response ◽  
Morus Nigra

Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia and increased oxidative stress. The treatment withMorus nigracan improve oxidative stress and complications due to diabetes.

scholarly journals Phytochemical, Antinutrients, Food Toxicants Analysis and Comparative Efficacy of Methanol Extracts of Selected Vegetables of Adamawa State Nigeria on the Haematological Parameters of Alloxan Induced Diabetic Rats

2021 ◽  
Vol 2 (1) ◽  
pp. 1-23
Author(s):  
Nwankwo Rita Ngozi ◽  
Ocheola-Oki Joyce Ene ◽  
Monsurat Moshod Tunrayo
Keyword(s):  
Diabetes Mellitus ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Hibiscus Cannabinus ◽  
Leafy Vegetables ◽  
Haematological Parameters ◽  
Albino Rats ◽  
Adansonia Digitata ◽  
Standard Drug ◽  
Methanol Extracts

Purpose: Phytochemicals present in vegetables has been associated with the health potentials of leafy vegetables. Diabetes has been implicated with increased oxidative stress leading to alteration of haematological parameters among other complications. This study investigated the phytochemicals, antinutrients and toxicants contents of methanol extracts of Hibiscus cannabinus, Adansonia digitata, Sesamum indicum and Cassia tora leaves and compared the effect of their leaves extract on some haematological parameters of alloxan – induced diabetic rats using standard methods of analysis.Methodology: Fifty-five (55) male albino rats weighing 120 – 150g divided into 11 groups of five rats each were used.  One diabetic untreated rat group, one diabetic group treated with standard drug ,8 diabetic groups treated with graded doses of the vegetable extracts and one normal group as control. All the groups received water and feed ad libitum together with their various treatments for 23 days. At the end of the experiment, blood was taken from the rats for determination of PCV, Hb, WBC, and RBC. ANOVA was used to separate the means of all the data collected with significance at p< 0.05 using Statistical Package for Scientist and Engineers (SPSE) version 9.1.Results: Results showed reasonable levels of phytochemicals, antinutrient and toxicants levels within WHO safe limits. Results of animal studies showed that administration of 500mg of Hibiscus cannabinus leaf extract increased the PCV, HB, WBC and RBC of diabetic rats more than all the other vegetable extracts (p<0.05). It also improved the WBC and RBC better than standard drug. This shows that Hibiscus cannabinus leaf extract might improve some haematological parameters associated with diabetes mellitus, however its inhibitory effect on higher dose raised concern for further investigation.Unique contribution to theory, practice and policy: Hibiscus cannabinus leaf extract could be used in the dietary management of haematological abnormalities associated with the pathophysiology of diabetes mellitus by health care systems

scholarly journals Hypoglycemic, Hypolipidemic and Antioxidant Activities of Ocimum gratissimum Leaf Extract on Diabetic Rats

2021 ◽  
pp. 25-40
Author(s):  
Na’ima Musa Sani ◽  
Abdulhamid Abubakar ◽  
Nwagu Jude
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Lipid Profile ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Standard Drug ◽  
Ocimum Gratissimum ◽  
Pancreatic Islets Of Langerhans

Aims: Ocimum gratissimum is a medicinal plant that has been traditionally used in the management of many diseases including diabetes mellitus. The aim of this research was to evaluate hypoglycemic, hypolipidemic and antioxidant activities of Ocimum gratissimum leaf extracts on diabetic rats. Study Design: Mention the design of the study here. Place and Duration of Study: Department of Biochemistry, Faculty of Life Science, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria. Between february 2021 and June 2021. Methodology: The phytochemical screening was carried out using standard procedures. The extract was administered orally (100, 200 and 400 mg/kg body weight, for 21 days) to alloxan-induced diabetic rats. Hypoglycemic effects, change in body weight, lipid profile and antioxidant activities of diabetic rats treated with the extract were assessed and compared with normal, diabetic control and standard drug treated rats. Histological examination of the pancreas during 21 days of treatment was also carried out. Results: The extract produced a significant reduction in fasting blood glucose level in alloxan-induced diabetic rats. Significant differences were also observed in body weights, serum lipid profile and levels of antioxidant vitamins and enzymes of Ocimum gratissimum methanol extract treated diabetic rats, when compared with diabetic, normal and standard drug treated rats. Histopathological studies of the pancreas showed comparable regeneration of the cells by extract which were earlier necrosed by alloxan. Methanol leaf extract of Ocimum gratissimum exhibit significant hypoglycemic, hypolipidemic and antioxidant activities in alloxan-induced diabetes in rats. The extract could be further processed towards the management of diabetes mellitus. Conclusion: Ocimum gratissimum extract showed a promising good hypoglycemic effect, the extracts also exhibit hypolipidemic and antioxidant activities on diabetic rats. There was regeneration of pancreatic islets of Langerhans. Therefore at acute dose the extracts can serve as an alternative in the management of diabetes mellitus.

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

scholarly journals Allopurinol ameliorates liver injury in type 1 diabetic rats through activating Nrf2

2021 ◽  
Vol 35 ◽  
pp. 205873842110314
Author(s):  
Fei Zeng ◽  
Jierong Luo ◽  
Hong Han ◽  
Wenjie Xie ◽  
Lingzhi Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Caspase 3 ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Heme Oxygenase 1 ◽  
Liver Protein ◽  
Lipid Peroxidation Product ◽  
Protein Expressions

Hyperglycemia-induced oxidative stress plays important roles in the development of non-alcoholic fatty liver disease (NAFLD), which is a common complication in diabetic patients. The Nrf2-Keap1 pathway is important for cell antioxidant protection, while its role in exogenous antioxidant mediated protection against NAFLD is unclear. We thus, postulated that antioxidant treatment with allopurinol (ALP) may attenuate diabetic liver injury and explored the underlying mechanisms. Control (C) and streptozotocin (STZ)-induced diabetes rats (D) were untreated or treated with ALP for 4 weeks starting at 1 week after diabetes induction. Serum levels of alanine aminotransferase (ALT) and aspartate transaminase (AST), production of lipid peroxidation product malondialdehyde (MDA), and serum superoxide dismutase (SOD) were detected. Liver protein expressions of cleaved-caspase 3, IL-1β, nuclear factor-erythroid-2-related factor-2 (Nrf2), heme oxygenase-1 (HO-1), P62, Kelch-like ECH-associated protein 1 (Keap1), and LC3 were analyzed. In vitro, cultured rat normal hepatocytes BRL-3A were grouped to normal glucose (5.5 mM, NG) or high glucose (25 mM, HG) and treated with or without allopurinol (100 µM) for 48 h. Rats in the D group demonstrated liver injury evidenced as increased serum levels of ALT and AST. Diabetes increased apoptotic cell death, enhanced liver protein expressions of cleaved-caspase 3 and IL-1β with concomitantly increased production of MDA while serum SOD content was significantly reduced (all P < 0.05 vs C). In the meantime, protein levels of Nrf2, HO-1, and P62 were reduced while Keap1 and LC3 were increased in the untreated D group as compared to control ( P < 0.05 vs C). And all the above alterations were significantly attenuated by ALP. Similar to our findings obtained from in vivo study, we got the same results in in vitro experiments. It is concluded that ALP activates the Nrf2/p62 pathway to ameliorate oxidative stress and liver injury in diabetic rats.

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