scholarly journals Rapid Determination of Sotolon in Fortified Wines Using a Miniaturized Liquid-Liquid Extraction Followed by LC-MS/MS Analysis

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Vanda Pereira ◽  
João M. Leça ◽  
João M. Gaspar ◽  
Ana C. Pereira ◽  
José C. Marques
Keyword(s):  
Rapid Determination ◽  
High Sensitivity ◽  
Liquid Extraction ◽  
Single Step ◽  
Alcoholic Beverages ◽  
Liquid Liquid Extraction ◽  
Sweet Wines ◽  
Fortified Wines ◽  
Full Factorial

Sotolon (4,5-dimethyl-3-hydroxy-2,5-dihydrofuran-2-one) is a powerful odorant usually pointed out as being responsible not only for the characteristic curry notes of the finest fortified wines but also for the off-flavour notes in prematurely oxidized white wines. Most methods reported in literature for quantifying sotolon in wines are quite laborious and use large volumes of organic solvents. Thus, in the present study, the development of a simple, fast, and environment-friendly method for the quantification of sotolon in fortified wine is herein presented. The proposed method uses a single-step liquid-liquid extraction followed by RP-LC-MS/MS and was optimized using a full factorial design. The method showed good linearity (R2 = 0.9999), intra- and interday precision lower than 10% RSD, recovery of about 95%, and high sensitivity (LOQ of 0.04 μg/L). The method was applied to analyse 44 fortified wines from different styles (from dry to sweet wines) and ages (3–115 years old), and it was found that it covers the concentration range usually found for this compound in this kind of alcoholic beverages, which was found to be within 6.3–810 μg/L. Thus, it can be concluded that this method can be used as an accurate tool for the rapid analysis of sotolon, since the early stages of its formation up to long ageing periods.

Determination of Benzaldehyde in Wines and Amyl Acetate and Isoamyl Acetate in Liqueurs by Gas-Liquid Chromatography and Confirmation by Mass Spectroscopy

1975 ◽  
Vol 58 (3) ◽  
pp. 582-584
Author(s):  
Glenn E Martin ◽  
Peter C Buscemi ◽  
Boyd B Butts
Keyword(s):  
Liquid Chromatography ◽  
Mass Spectroscopy ◽  
Isoamyl Acetate ◽  
Liquid Extraction ◽  
Amyl Acetate ◽  
Alcoholic Beverages ◽  
Gas Liquid Chromatography ◽  
Liquid Liquid Extraction ◽  
Liquid Chromatographic

Abstract Liquid-liquid extraction was carried out prior to gas-liquid chromatographic (GLC) determination of amyl and isoamyl acetate in alcoholic beverages. The concentrations of amyl acetate and isoamyl acetate ranged from 4 to 29 and 2.5 to 322 ppm, respectively. Recovery studies for isoamyl and amyl acetate were carried out; these compounds were recovered at 90 and 92%, respectively. A liquid-liquid extraction also was carried out prior to the GLC determination of benzaldehyde. When chloroform was used in the liquid extraction, the results obtained by the GLC procedure compared favorably in most cases with those obtained by the ultraviolet spectrophotometric procedure. In the GLC method the benzaldehyde varied from 5 to 150 ppm for both imported and natural wines. The compounds in the effluent from the chromatograph, namely, amyl acetate, isoamyl acetate, and benzaldehyde, were confirmed by mass spectroscopy.

scholarly journals Development and Validation of UPLC-MS/MS Method for the Determination of Aripiprazole in Rat Plasma Using Liquid-Liquid Extraction: Pharmacokinetic and Bioequivalence Application

2020 ◽  
Vol 32 (10) ◽  
pp. 2671-2676
Author(s):  
Ashish Raghuvanshi ◽  
Urooj A. Khan ◽  
Uzma Parveen ◽  
Anshul Gupta ◽  
Gaurav K. Jain
Keyword(s):  
Internal Standard ◽  
Rat Plasma ◽  
Liquid Extraction ◽  
Single Step ◽  
Liquid Liquid Extraction ◽  
Validation Parameters ◽  
Tandem Mass Spectrometric ◽  
Liquid Chromatographic

A selective, simple, sensitive and rapid ultra-performance liquid chromatographic tandem mass spectrometric (UPLC-MS/MS) method for the detection of aripiprazole in rat plasma has been developed and validated using aripiprazole-D8 as internal standard (IS). A simple single step sample preparation process was accomplished by liquid-liquid extraction (LLE). The post-treatment samples were chromato-graphed and analyzed on a UPLC bridged ethyl hybrid (BEH) C-18 column using mobile phase composition of acetonitrile: 0.1% formic acid in water::70:30 (v/v). Aripiprazole was analyzed by MS detector in positive electrospray ionization mode (ESI). Multiple reactions monitoring (MRM) was employed to observed the transition for aripiprazole (m/z 448.35→285.09) and aripiprazole-D8 (m/z 456.2→293.2). The developed method was validated and found linear in the working range of 2-1025 ng/mL with correlation coefficient, r2 = 0.99951 and quantification limit of 2.02 ng/mL. All validation parameters were in accordance with the ICH guidelines and met the acceptance criteria. The method was found to be accurate (recovery, 97.07 to 103.64%, precise (% CV, 2.68 to 7.70%), rapid (run time 4 min) and specific. The validated method was successfully used for the determination of plasma concentration of aripiprazole after single oral administration in rats and hence could be useful for in vivo pharmacokinetic study and bioequivalence testing of aripiprazole formulations.

Note on a Rapid Determination of Aflatoxins in Peanuts and Peanut Products

1970 ◽  
Vol 53 (5) ◽  
pp. 1064-1066
Author(s):  
D H Liem ◽  
P R Beljaars
Keyword(s):  
Rapid Determination ◽  
Peanut Butter ◽  
Extraction Procedure ◽  
Liquid Extraction ◽  
Direct Extraction ◽  
Roasted Peanut ◽  
Rapid Extraction ◽  
Liquid Liquid Extraction ◽  
Extracting Solvent

Abstract A simple and rapid extraction procedure, based on a direct extraction introduced by Pons and a single liquid-liquid extraction proposed by Waltking, is described for peanuts and peanut products. Methanol is used as the extracting solvent. TLC, using the system described by Engstrom, yielded satisfactory results. The method is suitable for aflatoxin Bi levels of 8 μg/kg (or higher) in peanut butter and roasted peanut products. Aflatoxin B1 levels down to 3 μg/kg can be detected in raw peanuts and peanut shavings.

scholarly journals A Microfluidic Platform for the Rapid Determination of Distribution Coefficients by Gravity-Assisted Droplet-Based Liquid–Liquid Extraction

2015 ◽  
Vol 87 (12) ◽  
pp. 6265-6270 ◽  
Author(s):  
Carl Esben Poulsen ◽  
Robert C. R. Wootton ◽  
Anders Wolff ◽  
Andrew J. deMello ◽  
Katherine S. Elvira
Keyword(s):  
Rapid Determination ◽  
Distribution Coefficients ◽  
Liquid Extraction ◽  
Microfluidic Platform ◽  
Liquid Liquid Extraction
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