scholarly journals The Protective Effect of New Compound XH-103 on Radiation-Induced GI Syndrome

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Yinping Dong ◽  
Ying Cheng ◽  
Qinlian Hou ◽  
Jing Wu ◽  
Deguan Li ◽  
...  
Keyword(s):  
Survival Rate ◽  
Protective Effect ◽  
Morphological Changes ◽  
Intestinal Injury ◽  
Intestinal Morphology ◽  
Small Intestinal Injury ◽  
Radiation Induced ◽  
Small Intestinal ◽  
Total Body ◽  
Crypt Cells

Background. Radiation-induced intestinal injury is one of the side effects in patients receiving radiotherapy. The aim of the present study was to investigate the protective effect of XH-103 on radiation-induced small intestinal injury and to explore its mechanism. Methods. C57BL/6N mice were irradiated and treated with XH-103. Firstly, the survival rate of mice exposed to 9.0 Gy and 11.0 Gy total body irradiation (TBI) was examined. Subsequently, at 3.5 d after IR, the small intestinal morphological changes were examined by HE. The numbers of crypt cells, the villus height, the expression of Ki67 and Lgr5, and the apoptotic cells in the intestinal crypts were examined by immunohistochemistry. Furthermore, the expression of p53 and Bax was analyzed by WB. Results. Compared to the irradiation group, XH-103 improved the mice survival rate, protected the intestinal morphology of mice, decreased the apoptotic rate of intestinal crypt cells, maintained cell regeneration, and promoted crypt proliferation and differentiation. XH-103 also reduced the expression of p53 and Bax in the small intestine compared to the IR group. Conclusion. These data demonstrate that XH-103 can prevent radiation-induced intestinal injury, which is beneficial for the protection of radiation injuries.

scholarly journals Protective effects of zingerone derivate on ionizing radiation-induced intestinal injury

2019 ◽  
Vol 60 (6) ◽  
pp. 740-746 ◽  
Author(s):  
Jing Wu ◽  
Yuqing Duan ◽  
Jie Cui ◽  
Yinping Dong ◽  
Hongyan Li ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Morphological Changes ◽  
Lethal Dose ◽  
Intestinal Injury ◽  
Intestinal Morphology ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Intestinal Crypt ◽  
Proliferation And Differentiation ◽  
Crypt Cells

Abstract Intestinal injury is the primary toxicity of radiotherapy for pelvic and abdominal tumors, and it is also one of the common acute complications of radiotherapy. At present, there are no effective drugs to prevent intestinal injury in the clinic. Zingerone is a natural product with radioprotective effects. In this study, a novel compound (thiazolidine hydrochloride, TZC01) was synthesized by structural modification of zingerone. The effects of TZC01 on preventing intestinal injury from radiation were further investigated in this study. C57BL/6N mice were exposed to a lethal dose of abdominal irradiation (ABI) with and without TZC01 treatments. The morphological changes of the intestine and various makers of intestinal crypt cells were investigated. Treatment with TZC01 improved the survival rate of mice exposed to 12 Gy ABI. Moreover, TZC01 protected the intestinal morphology of mice, decreased the apoptotic rate of intestinal crypt cells, maintained cell regeneration and promoted crypt cell proliferation and differentiation. This study suggests that TZC01 has preventive and therapeutic effects on radiation enteritis by promoting the proliferation and differentiation of crypt cells to protect the small intestine from the toxic effects of ionizing radiation. Furthermore, the study of TCZ01 lays a strong foundation for developing novel radioprotectors with multiple properties.

IL-13 Receptor α2 Promotes Epithelial Cell Regeneration From Radiation–Induced Small Intestinal Injury in Mice

2006 ◽  
Vol 131 (1) ◽  
pp. 130-141 ◽  
Author(s):  
Rei Kawashima ◽  
Yuki I. Kawamura ◽  
Rie Kato ◽  
Noriko Mizutani ◽  
Noriko Toyama–Sorimachi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Injury ◽  
Cell Regeneration ◽  
Small Intestinal Injury ◽  
Radiation Induced ◽  
Small Intestinal

scholarly journals The Protective Effect of 5-Aminosalicylic Acid against Non-Steroidal Anti-Inflammatory Drug-Induced Injury through Free Radical Scavenging in Small Intestinal Epithelial Cells

Medicina ◽  
2020 ◽  
Vol 56 (10) ◽  
pp. 515
Author(s):  
Eun Suk Jung ◽  
Hyun Joo Jang ◽  
Eun Mi Hong ◽  
Hye Li Lim ◽  
Sang Pyo Lee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Protective Effect ◽  
Cell Apoptosis ◽  
Radical Scavenging ◽  
Intestinal Injury ◽  
Activity Measurement ◽  
Aminosalicylic Acid ◽  
Small Intestinal Injury ◽  
Small Intestinal ◽  
Anti Inflammatory

Background and objectives: Non-steroidal anti-inflammatory drugs (NSAIDs) have been among the major causes of small intestinal injury in clinical practice. As such, the current study investigated the protective effect of 5-aminosalicylic acid (5-ASA) against an NSAID-induced small intestinal injury. Materials and Methods: IEC-6 cells were treated with various concentrations of indomethacin with or without 5-ASA in a serum-free medium, after which an 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Dromide (MTT) assay, a cell apoptosis assay, a caspase-3 activity assay, a reactive oxygen species (ROS) content and Superoxide dismutase 2 (SOD2) activity measurement, a Western blotting for occludin and zonula occludens-1 (ZO-1) and a wound healing assay were conducted. Results: 5-ASA ameliorated indomethacin-induced cell apoptosis and an increase in the intracellular ROS content while augmenting the indomethacin-induced suppression of SOD2 activity in IEC-6 cells. Moreover, 5-ASA reversed the indomethacin-induced attenuation of occludin and ZO-1 expression and promoted faster wound healing effects in IEC-6 cells following an indomethacin-induced injury. Conclusions: Our results suggested that 5-ASA protects small intestinal cells against an NSAID-induced small intestinal injury by scavenging free radicals. Therefore, 5-ASA could be a potential treatment for an NSAID-induced small intestinal injury.

scholarly journals Anti-Oxidative and Immuno-Protective Effect of Camel Milk on Radiation-Induced Intestinal Injury in C57BL/6 J Mice

Dose-Response ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 155932582110037
Author(s):  
Yu-Zhong Chen ◽  
Chao Li ◽  
Jia Gu ◽  
Si-chen Lv ◽  
Jia-ying Song ◽  
...  
Keyword(s):  
Survival Rate ◽  
Protective Effect ◽  
Survival Time ◽  
Intestinal Injury ◽  
Camel Milk ◽  
Whole Body ◽  
Inflammatory Factors ◽  
Control Group ◽  
Irradiation Group ◽  
Radiation Induced

Purpose: The main objective is to investigate the protective effect of camel milk (CM) on radiation-induced intestinal injury. Methods: The C57BL/6 J mice in 2 experiments were assigned into control group (Con), irradiation group (IR), and CM+irradiation group (CM+IR). After receiving the CM via gavage for 14 days, the mice in the first experiment were exposed to 6 Gy X-ray whole body irradiation, and survival rate was compared among the groups. Mice in the second experiment were exposed to 4 Gy irradiation and sacrificed at day 7. The small intestines were collected to examine the histopathological changes and to determine the anti-oxidative index and HMGB1/TLR4 inflammatory pathway. Fasting blood was used to measure serum pro-inflammatory factors. Results: Compared with the IR group, the survival time was prolonged, and survival rate was increased in the CM+IR group. CM increased levels of SOD and GSH and decreased MDA in the jejunum. Furthermore, intestinal protein expression of HMGB1/TLR4 pathway (TLR4, NF-κB, and HMGB1) was up-regulated by CM intervention. CM decreased the serum levels of TNF-α and IL-1β and increased IL-10 level. Conclusions: CM extended the survival time and had a protective effect against radiation-induced jejunum injury by regulation of antioxidant capacity and HMGB1/TLR4/NF-κB/MyD88 inflammatory signaling pathway.

scholarly journals Prevention of Traditional NSAID-Induced Small Intestinal Injury: Recent Preliminary Studies Using Capsule Endoscopy

Digestion ◽  
2010 ◽  
Vol 82 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Shunji Fujimori ◽  
Yoko Takahashi ◽  
Tsuguhiko Seo ◽  
Katya Gudis ◽  
Akihito Ehara ◽  
...  
Keyword(s):  
Capsule Endoscopy ◽  
Intestinal Injury ◽  
Traditional Nsaid ◽  
Small Intestinal Injury ◽  
Small Intestinal
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