scholarly journals “Turn-Off” Fluorescent Sensor for Pamidronate Disodium and Zoledronic Acid Based on Newly Synthesized Carbon Dots from Black Tea

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Peng Li ◽  
Yongcheng Hu
Keyword(s):  
Zoledronic Acid ◽  
Bone Metastases ◽  
Carbon Dots ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Black Tea ◽  
Rapid Analysis ◽  
Pamidronate Disodium

As common bisphosphonates drugs, pamidronate disodium and zoledronic acid have been widely investigated for bone metastases. In this paper, a new “turn-off” model based on carbon dots (CDs) from black tea was established to analyze the two kinds of bisphosphonate drugs, pamidronate disodium and zoledronic acid. Through the new sensor, both of drugs can be quantitative, respectively, with the limit of detection of 5 × 10−9 mol·L−1 and 6 × 10−9 mol·L−1. In addition, the fluorescence of newly prepared CDs can be quenched by two drugs with various degrees via photoinduced electron transfer, which can be perfectly used to distinguish them. Most importantly, this turn-off method has been employed to analyze the two drugs under the influence of foreign interference factors. This method provides a new view and guidance for the rapid analysis and recognition of drugs for bone metastases in vitro and in vivo.

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

scholarly journals Combined In Vitro and In Vivo Approaches to Propose a Putative Adverse Outcome Pathway for Acute Lung Inflammation Induced by Nanoparticles: A Study on Carbon Dots

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 180
Author(s):  
Maud Weiss ◽  
Jiahui Fan ◽  
Mickaël Claudel ◽  
Luc Lebeau ◽  
Françoise Pons ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Lung Inflammation ◽  
Adverse Outcome ◽  
Cellular Responses ◽  
Target Cells ◽  
Inflammasome Activation ◽  
Adverse Outcome Pathway ◽  
Acute Lung Inflammation

With the growth of nanotechnologies, concerns raised regarding the potential adverse effects of nanoparticles (NPs), especially on the respiratory tract. Adverse outcome pathways (AOP) have become recently the subject of intensive studies in order to get a better understanding of the mechanisms of NP toxicity, and hence hopefully predict the health risks associated with NP exposure. Herein, we propose a putative AOP for the lung toxicity of NPs using emerging nanomaterials called carbon dots (CDs), and in vivo and in vitro experimental approaches. We first investigated the effect of a single administration of CDs on mouse airways. We showed that CDs induce an acute lung inflammation and identified airway macrophages as target cells of CDs. Then, we studied the cellular responses induced by CDs in an in vitro model of macrophages. We observed that CDs are internalized by these cells (molecular initial event) and induce a series of key events, including loss of lysosomal integrity and mitochondrial disruption (organelle responses), as well as oxidative stress, inflammasome activation, inflammatory cytokine upregulation and macrophage death (cellular responses). All these effects triggering lung inflammation as tissular response may lead to acute lung injury.

scholarly journals Anti-Cancer Properties of Theaflavins

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 987
Author(s):  
Eric J. O’Neill ◽  
Deborah Termini ◽  
Alexandria Albano ◽  
Evangelia Tsiani
Keyword(s):  
Signaling Pathways ◽  
Cancer Progression ◽  
Treatment Strategies ◽  
B Cell Lymphoma ◽  
Black Tea ◽  
Phosphorylated Akt ◽  
Phenolic Components ◽  
Anti Cancer

Cancer is a disease characterized by aberrant proliferative and apoptotic signaling pathways, leading to uncontrolled proliferation of cancer cells combined with enhanced survival and evasion of cell death. Current treatment strategies are sometimes ineffective in eradicating more aggressive, metastatic forms of cancer, indicating the need to develop novel therapeutics targeting signaling pathways which are essential for cancer progression. Historically, plant-derived compounds have been utilized in the production of pharmaceuticals and chemotherapeutic compounds for the treatment of cancer, including paclitaxel and docetaxel. Theaflavins, phenolic components present in black tea, have demonstrated anti-cancer potential in cell cultures in vitro and in animal studies in vivo. Theaflavins have been shown to inhibit proliferation, survival, and migration of many cancer cellswhile promoting apoptosis. Treatment with theaflavins has been associated with increased levels of cleaved poly (ADP-ribose) polymerase (PARP) and cleaved caspases-3, -7, -8, and -9, all markers of apoptosis, and increased expression of the proapoptotic marker Bcl-2-associated X protein (Bax) and concomitant reduction in the antiapoptotic marker B-cell lymphoma 2 (Bcl-2). Additionally, theaflavin treatment reduced phosphorylated Akt, phosphorylated mechanistic target of rapamycin (mTOR), phosphatidylinositol 3-kinase (PI3K), and c-Myc levels with increased expression of the tumour suppressor p53. This review summarizes the current in vitro and in vivo evidence available investigating the anti-cancer effects of theaflavins across various cancer cell lines and animal models.

scholarly journals Spectrofluorimetric Determination of Hydrochlorothiazide by a Carbon Dots-Based Probe via Inner Filtering Effect and Resonance Rayleigh Scattering

2022 ◽  
Author(s):  
Ali Ghafarloo ◽  
Reza Sabzi ◽  
Naser Samadi ◽  
Hamed Hamishehkar
Keyword(s):  
Carbon Dots ◽  
Rayleigh Scattering ◽  
Limit Of Detection ◽  
Fluorescent Sensor ◽  
Emission Spectra ◽  
Cost Effective ◽  
Inner Filter Effect ◽  
Resonance Rayleigh Scattering ◽  
Excitation Spectra ◽  
One Pot

Synthesis of carbon dots (CDs) from natural resources not only enables green synthesis and production of environmentally friendly materials, but also provides a cost-effective probe as a fluorescence nanosensor. The proposed sensor introduces a unique one-pot hydrothermal CDs synthesis from alfalfa leaves, which is promising for sensing hydrochlorothiazide (HCTZ) via inner filter effect (IFE) and resonance Rayleigh scattering (RRS). The as-prepared CDs had wide emission spectra, excitation-dependent emission, high solubility, high stability, and visible fluorescence light with a quantum yield of up to 11%. The absorption of HCTZ overlapped with the excitation spectra of CDs. Therefore, CDs represented excellent quenching due to IFE when HCTZ was gradually added. Furthermore, this fluorescent sensor was successfully used to quantify HCTZ in the linear ranges (0.17-2.50 μg mL-1) with the limit of detection of 0.11 μg mL-1. The sensing system was simple as no surface functionalization was required for CDs, leading to less laborious steps and more cost-effective synthesis. The reaction time was short, i.e., less than 2 min, indicating a simple approach for rapid analysis of HCTZ. By optimizing conditions, successful measurements were carried out on pharmaceutical tablets.

Lysosome-targeted red-fluorescent carbon dots for turn-on detection of permanganate and pH in vivo and in vitro

2021 ◽  
pp. 130774
Author(s):  
Dan Chang ◽  
Zhonghua Zhao ◽  
Jianyang Feng ◽  
Yuye Xin ◽  
Yongxing Yang ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Turn On ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

scholarly journals Efficacy of Guanabenz Combination Therapy against Chronic Toxoplasmosis across Multiple Mouse Strains

2020 ◽  
Vol 64 (9) ◽  
Author(s):  
Jennifer Martynowicz ◽  
J. Stone Doggett ◽  
William J. Sullivan
Keyword(s):  
Limit Of Detection ◽  
Acute Infection ◽  
Antagonistic Effect ◽  
Mouse Strains ◽  
Chronic Infections ◽  
Content Type ◽  
Brain Cyst ◽  
The Brain

ABSTRACT Toxoplasma gondii, an obligate intracellular parasite that can cause life-threatening acute disease, differentiates into a quiescent cyst stage to establish lifelong chronic infections in animal hosts, including humans. This tissue cyst reservoir, which can reactivate into an acute infection, is currently refractory to clinically available therapeutics. Recently, we and others have discovered drugs capable of significantly reducing the brain cyst burden in latently infected mice, but not to undetectable levels. In this study, we examined the use of novel combination therapies possessing multiple mechanisms of action in mouse models of latent toxoplasmosis. Our drug regimens included combinations of pyrimethamine, clindamycin, guanabenz, and endochin-like quinolones (ELQs) and were administered to two different mouse strains in an attempt to eradicate brain tissue cysts. We observed mouse strain-dependent effects with these drug treatments: pyrimethamine-guanabenz showed synergistic efficacy in C57BL/6 mice yet did not improve upon guanabenz monotherapy in BALB/c mice. Contrary to promising in vitro results demonstrating toxicity to bradyzoites, we observed an antagonistic effect between guanabenz and ELQ-334 in vivo. While we were unable to completely eliminate the brain cyst burden, we found that a combination treatment with ELQ-334 and pyrimethamine impressively reduced the brain cyst burden by 95% in C57BL/6 mice, which approached the limit of detection. These analyses highlight the importance of evaluating anti-infective drugs in multiple mouse strains and will help inform further preclinical studies of cocktail therapies designed to treat chronic toxoplasmosis.

scholarly journals TIE2 Induces Breast Cancer Cell Dormancy and Inhibits the Development of Osteolytic Bone Metastases

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 868
Author(s):  
Florian Drescher ◽  
Patricia Juárez ◽  
Danna L. Arellano ◽  
Nicolás Serafín-Higuera ◽  
Felipe Olvera-Rodriguez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Metastases ◽  
Kinase Inhibitors ◽  
Disseminated Tumor Cells ◽  
Tyrosine Kinase Receptor ◽  
In Vivo Models ◽  
Hematopoietic Stem ◽  
Cell Dormancy

Breast cancer (BCa) cells disseminating to the bone can remain dormant and resistant to treatments for many years until relapsing as bone metastases. The tyrosine kinase receptor TIE2 induces the dormancy of hematopoietic stem cells, and could also induce the dormancy of BCa cells. However, TIE2 is also a target for anti-angiogenic treatments in ongoing clinical trials, and its inhibition could then restart the proliferation of dormant BCa cells in bone. In this study, we used a combination of patient data, in vitro, and in vivo models to investigate the effect of TIE2 in the dormancy of bone metastases. In BCa patients, we found that a higher TIE2 expression is associated with an increased time to metastases and survival. In vitro, TIE2 decreased cell proliferation as it increased the expression of cyclin-dependent kinase inhibitors CDKN1A and CDKN1B and arrested cells in the G0/G1 phase. Expression of TIE2 also increased the resistance to the chemotherapeutic 5-Fluorouracil. In mice, TIE2 expression reduced tumor growth and the formation of osteolytic bone metastasis. Together, these results show that TIE2 is sufficient to induce dormancy in vitro and in vivo, and could be a useful prognostic marker for patients. Our data also suggest being cautious when using TIE2 inhibitors in the clinic, as they could awaken dormant disseminated tumor cells.

Monitoring tricyclic antidepressant concentrations in serum by fluorescence polarization immunoassay compared with gas chromatography and HPLC

1994 ◽  
Vol 40 (6) ◽  
pp. 929-933 ◽  
Author(s):  
M L Rao ◽  
U Staberock ◽  
P Baumann ◽  
C Hiemke ◽  
A Deister ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Fluorescence Polarization ◽  
Tricyclic Antidepressants ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Tricyclic Antidepressant ◽  
Therapeutic Drug ◽  
Fluorescence Polarization Immunoassay

Abstract The fluorescence polarization immunoassay (FPIA) developed by Abbott to diagnose intoxication with tricyclic antidepressants was adapted for therapeutic drug monitoring and validated with chromatograpic methods to investigate its potential for this use. We compared serum concentrations of tricyclic antidepressants in vivo and in vitro obtained by FPIA with those by gas chromatography and HPLC. For amitriptyline, imipramine, clomipramine, and doxepin, the detection limit of the FPIA was 72, 71, 64, and 72 nmol/L (approximately 20 micrograms/L), respectively; that by gas chromatography was 18, 18, and 16 nmol/L (approximately 5 micrograms/L) for amitriptyline, imipramine and clomipramine, respectively; with HPLC the lower limit of detection for doxepin was 36 nmol/L (10 micrograms/L). The intra- and interassay CVs ranged from 3% to 6%. In patients being treated with amitriptyline, imipramine, clomipramine, and doxepin, at steady-state the correlation coefficients between FPIA and GC/HPLC results for split samples were 0.95, 0.92, 0.90 and 0.70, respectively. However, the slopes were close to unity only for amitriptyline and doxepin, being 0.6 for imipramine and 1.9 for clomipramine.

In vitro and in vivo evaluation of cationic liposomes containing zoledronic acid as anticancer agent

2016 ◽  
Vol 12 (2) ◽  
pp. 513
Author(s):  
Xinjun Cai ◽  
Zeng Wang ◽  
Congyao Wang ◽  
Huijun Zhou ◽  
Jianjun Ni ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Anticancer Agent ◽  
Cationic Liposomes ◽  
In Vivo Evaluation
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