scholarly journals Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Li-mei Lin ◽  
Ling-jia Zhao ◽  
Jing Deng ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Steepest Ascent ◽  
Penthorum Chinense Pursh ◽  
Ft Ir ◽  
Experimental Yield ◽  
Burman Design ◽  
Anti Inflammatory ◽  
Penthorum Chinense ◽  
Optimized Conditions ◽  
Plackett Burman Design

Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

DESIGN OF CULTURAL CONDITIONS FOR PRODUCTION OF CHONDROITINASE BY ASPERGILLUS NIGER USING DESIGN OF EXPERIMENTS

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (06) ◽  
pp. 24-32
Author(s):  
N Kasinathan ◽  
◽  
V. M. Subrahmanyam ◽  
V. R Josyula
Keyword(s):  
Design Of Experiments ◽  
Yeast Extract ◽  
Spinal Cord Injuries ◽  
Box Behnken Design ◽  
Medium Components ◽  
Cultural Conditions ◽  
Burman Design ◽  
Level Of Significance ◽  
Optimized Conditions ◽  
Plackett Burman Design

Chondroitinases are useful in treating cancer and spinal cord injuries. The work reported so far does not focus on production of fungal chondroitinase. The present study focuses on the optimization of cultural conditions using design of experiments for production of chondroitinase by a fungal isolate CHCS-2, obtained from humus containing poultry waste. The cultural conditions were optimized systematically using a one-factor-at-a-time (OFAT) approach, factorial design, Plackett-Burman design (PBD) and Box- Behnken design to identify the composition of the production medium and the level of the variables. The level of significance of the medium components selected by OFAT study viz., yeast extract, chondroitin, and phosphate, was analyzed by factorial studies. The effect of eleven minerals on the enzyme yield was studied by PBD. The interaction among the medium components selected using factorial and PBD viz., yeast extract, cobalt and aluminum was investigated using the Box-Behnken design (BBD). Under the optimized conditions, maximum chondroitinase yield was 22.5 U/mL.

scholarly journals Improved Production of Sublancin 168 Biosynthesized byBacillus subtilis168 Using Chemometric Methodology and Statistical Experimental Designs

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Shengyue Ji ◽  
Weili Li ◽  
Haiyun Xin ◽  
Shan Wang ◽  
Binyun Cao
Keyword(s):  
Soybean Meal ◽  
Incubation Temperature ◽  
Optimal Combination ◽  
Fermentation Condition ◽  
Box Behnken Design ◽  
Steepest Ascent ◽  
Burman Design ◽  
Fermentation Parameters ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced byBacillus subtilis168, is effective in killing specific microorganisms. However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture ofB. subtilis168 by using the method in the literature. Thus optimization of fermentation condition for efficiently producing sublancin 168 is required. Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment. Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C. The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achieved.

scholarly journals Optimization of phenol degradation by Antarctic bacterium Rhodococcus sp.

2020 ◽  
Vol 32 (6) ◽  
pp. 486-495 ◽  
Author(s):  
Tengku Athirrah Tengku-Mazuki ◽  
Kavilasni Subramaniam ◽  
Nur Nadhirah Zakaria ◽  
Peter Convey ◽  
Khalilah Abdul Khalil ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Phenol Degradation ◽  
Antarctic Bacterium ◽  
Burman Design ◽  
High Concentrations ◽  
Predicted Values ◽  
The Antarctic ◽  
Optimized Conditions ◽  
Plackett Burman Design ◽  
Central Composite

AbstractThis study focused on the ability of the Antarctic bacterium Rhodococcus sp. strain AQ5-14 to survive exposure to and to degrade high concentrations of phenol at 0.5 g l-1. After initial evaluation of phenol-degrading performance, the effects of salinity, pH and temperature on the rate of phenol degradation were examined. The optimum conditions for phenol degradation were pH 7 and 0.4 g l-1 NaCl at a temperature of 25°C (83.90%). An analysis using response surface methodology (RSM) and the Plackett-Burman design identified salinity, pH and temperature as three statistically significant factors influencing phenol degradation. The maximum bacterial growth was observed (optical density at 600 nm = 0.455), with medium conditions of pH 6.5, 22.5°C and 0.47 g l-1 NaCl in the central composite design of the RSM experiments enhancing phenol degradation to 99.10%. A central composite design was then used to examine the interactions among these three variables and to determine their optimal levels. There was excellent agreement (R2 = 0.9785) between experimental and predicted values, with less strong but still good agreement (R2 = 0.8376) between the predicted model values and those obtained experimentally under optimized conditions. Rhodococcus sp. strain AQ5-14 has excellent potential for the bioremediation of phenol.

scholarly journals Melamine Recognition: Molecularly Imprinted Polymer for Selective and Sensitive Determination of Melamine in Food Samples

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohadese Biabani ◽  
Azizollah Nezhadali ◽  
Ahmad Nakhaei ◽  
Hossein Nakhaei
Keyword(s):  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Food Samples ◽  
Quantitative Measurements ◽  
Sensitive Determination ◽  
Burman Design ◽  
Mip Sensor ◽  
Optimized Conditions ◽  
Plackett Burman Design

In this study, a sensitive and selective sensor is constructed to measure the melamine (MEL) using molecular imprinting polymer (MIP) technique. Chemical and electrochemical techniques are used to construct the MIP and quantitative measurements. The constructed sensor was modified with GO-Fe3O4@SiO2 nanocomposite. Screening and optimization of factors are done using statistical methods, including Plackett–Burman design (PBD) and central composite design (CCD). Under the optimized conditions, an MIP sensor showed a linear range from 5.0 × 10−7 to 1.0 × 10−5 M MEL concentration with a correlation coefficient (R2) of 0.9997. The limit of detection was obtained (0.028 µM) with a highly reproducible response (RSD 2.15%, n = 4). The electrochemical sensor showed good results for the determination of MEL in food samples.

scholarly journals Optimization of Acid Protease Production byAspergillus nigerI1 on Shrimp Peptone Using Statistical Experimental Design

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Rayda Siala ◽  
Fakher Frikha ◽  
Samiha Mhamdi ◽  
Moncef Nasri ◽  
Alya Sellami Kamoun
Keyword(s):  
Culture Conditions ◽  
Medium Composition ◽  
Acid Protease ◽  
Protease Production ◽  
Statistical Experimental Design ◽  
Initial Ph ◽  
Burman Design ◽  
The Cost ◽  
Optimized Conditions ◽  
Plackett Burman Design

Medium composition and culture conditions for the acid protease production byAspergillus nigerI1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH2PO4, and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized conditions, the experimental protease production (183.13 U mL−1) closely matched the yield predicted by the statistical model (172.57 U mL−1) withR2=0.914. Compared with the initial M1 medium on which protease production was 43.13 U mL−1, a successful and significant improvement by 4.25 folds was achieved in the optimized medium containing (g/L): hulled grain of wheat (HGW) 5.0; KH2PO41.0; NaCl 0.3; MgSO4(7H2O) 0.5; CaCl2(7H2O) 0.4; ZnSO40.1; Na2HPO41.6; shrimp peptone (SP) 1.0. The pH was adjusted at 5 and the temperature at30°C. More interestingly, the optimization was accomplished using two cheap and local fermentation substrates, HGW and SP, which may result in a significant reduction in the cost of medium constituents.

scholarly journals Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Xiujun Gao ◽  
Peisheng Yan ◽  
Xin Liu ◽  
Jianbing Wang ◽  
Jiajia Yu
Keyword(s):  
Antioxidant Activity ◽  
Regression Models ◽  
Antioxidant Activities ◽  
High Yield ◽  
Box Behnken Design ◽  
Steepest Ascent ◽  
Cultural Conditions ◽  
Total Antioxidant ◽  
Burman Design ◽  
Plackett Burman Design

To optimize the production conditions for exopolysaccharides with higher antioxidant activities fromXerocomus badiuscultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of4.588±0.346 g/L and a total antioxidant activity of2.956±0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

scholarly journals Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Vinayagam Ramesh ◽  
Vytla Ramachandra Murty
Keyword(s):  
Yeast Extract ◽  
Soluble Starch ◽  
Thermophilic Fungus ◽  
Steepest Ascent ◽  
Proposed Model ◽  
The Media ◽  
Burman Design ◽  
Humicola Grisea ◽  
Plackett Burman Design ◽  
Important Enzyme

Glucoamylase is an industrially important enzyme which converts soluble starch into glucose. The media components for the production of glucoamylase from thermophilic fungus Humicola grisea MTCC 352 have been optimized. Eight media components, namely, soluble starch, yeast extract, KH2PO4, K2HPO4, NaCl, CaCl2, MgSO4·7H2O, and Vogel’s trace elements solution, were first screened for their effect on the production of glucoamylase and only four components (soluble starch, yeast extract, K2HPO4, and MgSO4·7H2O) were identified as statistically significant using Plackett-Burman design. It was fitted into a first-order model (R2=0.9859). Steepest ascent method was performed to identify the location of optimum. Central composite design was employed to determine the optimum values (soluble starch: 28.41 g/L, yeast extract: 9.61 g/L, K2HPO4: 2.42 g/L, and MgSO4·7H2O: 1.91 g/L). The experimental activity of 12.27 U/mL obtained was close to the predicted activity of 12.15. High R2 value (0.9397), low PRESS value (9.47), and AARD values (2.07%) indicate the accuracy of the proposed model. The glucoamylase production was found to increase from 4.57 U/mL to 12.27 U/mL, a 2.68-fold enhancement, as compared to the unoptimized medium.

scholarly journals The reduction of oil pollutants of petroleum products storage-tanks sludge using low-cost adsorbents

2017 ◽  
Vol 23 (2) ◽  
pp. 237-244
Author(s):  
Zahra Mokhtari-Hosseini ◽  
Razieh Hosseinabadi ◽  
Ashrafalsadat Zarmi
Keyword(s):  
Environmental Concern ◽  
Low Cost ◽  
Freundlich Isotherm ◽  
Petroleum Products ◽  
High Concentration ◽  
Oil And Grease ◽  
Burman Design ◽  
Optimized Conditions ◽  
Plackett Burman Design ◽  
Maximum Removal

Disposal of storage tank sludge in oil depots is a major environmental concern due to the high concentration of hydrocarbons involved. This paper investigates the reduction of the sludge oil pollutants with initial oil and grease concentration of about 50 mass% using low cost adsorbents. Among the examined adsorbents, sawdust indicated the maximum removal of oil and grease. The screening and optimizing of process parameters were evaluated employing Plackett-Burman design and response surface method. For the optimized conditions, more than 60 mass% of oil and grease from the sludge was removed. Moreover, it was found that sawdust adsorption of the oil and grease approximately followed the Freundlich isotherm. The results indicated that oil pollutants of sludge could be reduced using sawdust as a low-cost, available and flammable adsorbent so that thus saturated adsorbents could be used as fuel in certain industries.

scholarly journals Application of Plackett Burman Design for Antioxidant Extraction from Actinodaphne sesquipedalis leaves

2019 ◽  
Vol 8 (6S3) ◽  
pp. 48-51
Keyword(s):  
Microwave Power ◽  
Ethanol Concentration ◽  
Irradiation Time ◽  
Radical Scavenging Activity ◽  
Power Level ◽  
Radical Scavenging ◽  
Solvent Ratio ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Microwave Power Level

Actinodaphne sesquipedalis Hook. F. Var. Glabra (Kochummen), also known as “Medang payung” by the Malay people, belongs to the Lauraceae family. In this study, a Plackett-Burman design was used to evaluate the significant extraction parameters in achieving maximum DPPH radical scavenging activity from ethanol leaves extract of A. sesquipedalis. Microwave-assisted extraction technique was employed using aqueous ethanol. The independent parameters were microwave power level (30–60 W), feed-to-solvent ratio (1:30 g/ml), irradiation time (30-90 s) and ethanol concentration (20–80%). Amongst the examined parameters, ethanol concentration, microwave power level, and irradiation/extraction time were significant, whereas, feed-to-solvent ratio was insignificant. Therefore, antioxidants from the ethanolic extraction leaves of A. sesquipedalis using microwave technique are significantly affected by ethanol concentration, irradiation time and microwave power.

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