scholarly journals Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Ouko Abigael ◽  
Okoth Sheila ◽  
Amugune Nelson ◽  
Vesa Joutsjoki
Keyword(s):  
Aspergillus Flavus ◽  
Mating Type ◽  
High Mobility ◽  
Aflatoxin Production ◽  
High Mobility Group Protein ◽  
Group Protein ◽  
Mating Type Genes ◽  
Polymerase Chain ◽  
Mat Genes

In this study, the possibility of sexual reproduction in sampled Aspergillus flavus strains was evaluated by assessing the distribution of mating type (MAT) genes, which are known to control sexual character among fungi, for two counties in Kenya. Forty-four isolates from Nandi and Makueni counties were genotyped by MAT using a multiplex polymerase chain reaction assay. The primer pair for the MAT1-1 amplified a 396 base pair (bp) fragment containing an α-box motif, and MAT1-2 primers targeted a 270 bp segment with a high mobility group protein. The MAT1-2 genes dominated in both regions although the frequency was higher in Nandi (75%) than in Makueni (54.17%). There were no MAT1-1 genes sampled in Nandi, and in Makueni their proportion was 15.91%. The percentage of isolates that amplified for both MAT genes in Makueni was 9.09%, while in Nandi it was 11.36%. Currently, use of aggressive aflatoxin non-producing A. flavus strains as biocontrol is the most promising preharvest aflatoxin control strategy in Kenya. However, we address the possibility of introduced biocontrol strains to breed with existing aflatoxin producing strains in nature, which could lead to the generation of A. flavus offspring capable of aflatoxin production while also being aggressive colonizers and possibly increasing the burden of aflatoxin exposure in food.

scholarly journals Mating-Type Genes from Asexual Phytopathogenic Ascomycetes Fusarium oxysporum and Alternaria alternata

2000 ◽  
Vol 13 (12) ◽  
pp. 1330-1339 ◽  
Author(s):  
Tsutomu Arie ◽  
Isao Kaneko ◽  
Takanobu Yoshida ◽  
Masami Noguchi ◽  
Yoshikuni Nomura ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Mating Type ◽  
Alternaria Alternata ◽  
High Mobility ◽  
Hmg Box ◽  
Starting Point ◽  
Mating Type Genes ◽  
Polymerase Chain ◽  
Flanking Regions ◽  
Mat Genes

Mating-type (MAT) loci were cloned from two asexual (mitosporic) phytopathogenic ascomycetes, Fusarium oxysporum (a pyrenomycete) and Alternaria alternata (a loculoascomycete), by a polymerase chain reaction (PCR)-based strategy. The conserved high mobility group (HMG) box domain found in the MAT1-2-1 protein was used as a starting point for cloning and sequencing the entire MAT1-2 idiomorph plus flanking regions. Primer pairs designed to both flanking regions were used to amplify the opposite MAT1-1 idiomorph. The MAT1-1 and MAT1-2 idiomorphs were approximately 4.6 and 3.8 kb in F. oxysporum and approximately 1.9 and 2.2 kb in A. alternata, respectively. In both species, the MAT1-1 idiomorph contains at least one gene that encodes a protein with a putative alpha box domain and the MAT1-2 idiomorph contains one gene that encodes a protein with a putative HMG box domain. MAT-specific primers were used to assess the mating type of F. oxysporum and A. alternata field isolates by PCR. MAT genes from A. alternata were expressed. The A. alternata genes were confirmed to be functional in a close sexual relative, Cochliobolus heterostrophus, by heterologous expression.

scholarly journals Characterization of high mobility group protein levels during spermatogenesis in the rat.

1984 ◽  
Vol 259 (14) ◽  
pp. 8840-8846
Author(s):  
L R Bucci ◽  
W A Brock ◽  
I L Goldknopf ◽  
M L Meistrich
Keyword(s):  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Protein Levels ◽  
Group Protein

Characterization of human SMARCE1r high-mobility-group protein

2002 ◽  
Vol 1574 (3) ◽  
pp. 269-276 ◽  
Author(s):  
Young Mi Lee ◽  
Hyunjin Shin ◽  
Wonja Choi ◽  
Sungmin Ahn ◽  
Wankee Kim
Keyword(s):  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Group Protein

Characterization of high mobility group protein binding to cisplatin-damaged DNA

1992 ◽  
Vol 188 (3) ◽  
pp. 1286-1294 ◽  
Author(s):  
Paul C. Billings ◽  
Richard J. Davis ◽  
Beatrice N. Engelsberg ◽  
Kirsten A. Skov ◽  
Edward N. Hughes
Keyword(s):  
Protein Binding ◽  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Group Protein ◽  
Damaged Dna

scholarly journals Novel Reference Gene, High-mobility-group protein I/Y, Used in Qualitative and Real-Time Quantitative Polymerase Chain Reaction Detection of Transgenic Rapeseed Cultivars

2005 ◽  
Vol 88 (2) ◽  
pp. 577-584 ◽  
Author(s):  
Haibo Weng ◽  
Litao Yang ◽  
Zhili Liu ◽  
Jiayu Ding ◽  
Aihu Pan ◽  
...  
Keyword(s):  
Reference Gene ◽  
High Mobility ◽  
Quantitative Detection ◽  
High Mobility Group Protein ◽  
Qualitative And Quantitative ◽  
Qualitative And Quantitative Pcr ◽  
Group Protein ◽  
Endogenous Reference ◽  
Polymerase Chain ◽  
Transgenic Rapeseed

Abstract With the development of transgenic crops, regulations to label the genetically modified organisms (GMOs) and their derived products have been issued in many countries. Polymerase chain reaction (PCR) methods are thought to be reliable and useful techniques for qualitative and quantitative detection of GMOs. These methods are generally needed to amplify the transgene and compare the amplified results with that of a corresponding reference gene to get the reliable results. Specific primers were developed for the rapeseed (Brassica napus), High-mobility-group protein I/Y (HMG-I/Y) single-copy gene and PCR cycling conditions suitable for the use of this sequence as an endogenous reference gene in both qualitative and quantitative PCR assays. Both methods were assayed with 15 different rapeseed varieties, and identical amplified products were obtained with all of them. No amplification was observed when templates were the DNA samples from the other species of Brassica genus or other species, such as broccoli, stem mustard, cauliflower, Chinese cabbage, cabbage, sprouts, Arabidopsis thaliana, carrot, tobacco, soybean, mung bean, tomato, pepper, eggplant, plum, wheat, maize, barley, rice, lupine, and sunflower. This system was specific for rapeseed. Limits of detection and quantitation in qualitative and quantitative PCR systems were about 13 pg DNA (about 10 haploid genomes) and about 1.3 pg DNA (about 1 haploid genome), respectively. To further test the feasibility of this HMG-I/Y gene as an endogenous reference gene, samples containing transgenic rapeseed GT73 with the inserted glyphosate oxidoreductase (GOX) gene were quantitated. These demonstrated that the endogenous PCR detection systems were applicable to the qualitative and quantitative detection of transgenic rapeseed.

Isolation and characterization of the gene coding for murine high-mobility-group protein HMGI-C

Gene ◽  
1995 ◽  
Vol 167 (1-2) ◽  
pp. 249-253 ◽  
Author(s):  
Guidalberto Manfioletti ◽  
Alessandra Rustighi ◽  
Fiamma Mantovani ◽  
Graham H. Goodwin ◽  
Vincenzo Giancotti
Keyword(s):  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Isolation And Characterization ◽  
Gene Coding ◽  
Group Protein
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