scholarly journals Screening and Molecular Identification of Pectinase Producing Microbes from Coffee Pulp

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Oliyad Jeilu Oumer ◽  
Dawit Abate
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Selective Growth ◽  
Growth Media ◽  
Commercial Sector ◽  
Coffee Pulp ◽  
Pectinolytic Enzymes ◽  
Pectinase Activity ◽  
Global Food ◽  
Byproduct Utilization

Application of enzymes in biotechnological process has expanded considerably in recent years. In food and related industry, major importance was being attached to the use of enzymes in upgrading quality, increasing yields of extractive processes, product stabilization, and improvement of flavor and byproduct utilization. Pectinases or pectinolytic enzymes are today one of the upcoming enzymes of the commercial sector. It has been reported that microbial pectinases account for 25% of the global food enzymes sales. For this reason, this study was undertaken with aims of screening microorganisms for the pectinase activity from coffee pulp samples and molecular identification of the potential pectinolytic isolates. In the present investigation, in total, ninety-five (95) isolates were identified from thirty coffee pulp samples. Based on characterization on the selective growth media, the isolates were grouped as actinomycete (21.06%), bacteria (65.26%), and fungi (13.68%). Among these, 31.58% showed colonies surrounded by clear zones which indicate the presence of pectinase activity. After rigorous screening steps, the isolates with high potential pectinase activity were identified molecularly by sequencing 16S rDNA region of the isolates. Based on the molecular identifications, about 70% of the isolates are under genus Bacillus.

scholarly journals Improved molecular identification of Thermoactinomyces spp. associated with mushroom worker's lung by 16S rDNA sequence typing

2002 ◽  
Vol 51 (12) ◽  
pp. 1117-1127 ◽  
Author(s):  
JIRU XU ◽  
JAMES EVANS ◽  
J. STUART ELBORN ◽  
JOHN E. MOORE ◽  
JOHN G. BARR ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Rdna Sequence ◽  
16S Rdna Sequence

scholarly journals ISOLATION AND MOLECULAR IDENTIFICATION OF FRESHWATER MICROALGAE IN MANINJAU LAKE WEST SUMATERA

2018 ◽  
Author(s):  
zulkarnain chaidir ◽  
Neri Fadjria ◽  
Armaini ◽  
Rahadian Zainul
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
18S Rdna ◽  
Gene Bank ◽  
Freshwater Microalgae ◽  
Eukaryotic Microorganisms ◽  
West Sumatra ◽  
Percent Similarity

Microalgae are photosynthetic prokaryotic and eukaryotic microorganisms. In this research, isolation of microalgae from Maninjau lake West Sumatra was identified by morphological and molecular identification by PCR using primers for 16S rDNA and 18S rDNA prokaryotic and eukaryotic microalgae. The results of this study were obtained 3 isolates of microalgae that can be isolated from Maninjau lake West Sumatra are Scenedesmus (code isolate AUMA-020) with a percent similarity of 95%, Uncultured cyanobacterium (code isolates AUMA-023) with a percent similarity of 80% and Limnothrix (code isolates AUMA-019) has a percent similarity of 98% based on data gene Bank blast results.

scholarly journals Identifikasi Molekuler Bakteri Endofit Penghasil L-asparaginase yang Diisolasi dari Mangrove Buta-Buta (Excoecaria agallocha)

2019 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Asep Awaludin Prihanto ◽  
Randy Fahrudin Ardiansyah ◽  
Ken Audia Pradarameswari
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Endophytic Bacteria ◽  
Sequence Data ◽  
Enterobacter Cloacae ◽  
Selective Medium ◽  
Mangrove Plant ◽  
Excoecaria Agallocha ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.

Molecular identification of the first human isolate belonging to the Veillonella ratti–Veillonella criceti group based on 16S rDNA and dnaK gene sequencing

2005 ◽  
Vol 156 (4) ◽  
pp. 603-607 ◽  
Author(s):  
Hélène Marchandin ◽  
Corinne Teyssier ◽  
Estelle Jumas-Bilak ◽  
Maxime Robert ◽  
Anne-Catherine Artigues ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Gene Sequencing ◽  
Human Isolate

scholarly journals Isolation and molecular identification of Vibrio spp. by sequencing of 16S rDNA from seafood, meat and meat products in Libya

2016 ◽  
Vol 6 (1) ◽  
pp. 36 ◽  
Author(s):  
SM Azwai ◽  
EA Alfallani ◽  
SK Abolghait ◽  
AM Garbaj ◽  
HT Naas ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Meat Products
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