scholarly journals Further Validation of a Rapid Screening Semiquantitative Thin-Layer Chromatographic Method for Marketed Antimalarial Medicines for Adoption in Malawi

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Dorcas Osei-Safo ◽  
Ibrahim Chikowe ◽  
Jerry Joe Ebow Kingsley Harrison ◽  
Daniel Konadu Yeboah ◽  
Ivan Addae-Mensah
Keyword(s):  
Thin Layer ◽  
Screening Tool ◽  
Retention Factor ◽  
Essential Medicines ◽  
Rapid Screening ◽  
Hplc Assay ◽  
Pharmaceutical Ingredients ◽  
Accuracy And Precision ◽  
Field Estimation

A recently developed semiquantitative thin-layer chromatographic (SQ-TLC) assay has been employed in postmarketing surveillance of antimalarial medicines used in Malawi prior to HPLC assay. Both methods gave analogous results in a significant majority of the samples, with a good correlation (r = 0.9012) for the active pharmaceutical ingredients of the dosage forms assayed. Artemether-containing medicines had the highest percentage (92.67%) of samples with comparable results for both assays. The lowest percentage (66.67%) was observed in artesunate-containing medicines. The SQ-TLC method was validated for specificity, accuracy, precision, linearity, and stability according to the International Conference on Harmonisation guidelines, with the results falling within acceptable limits. For specificity, retention factor values of the test samples and reference standards were comparable, while accuracy and precision of 91.1 ± 5.7% were obtained for all samples. The method was linear in the range 1.0–2.0 µg/spot with a correlation coefficient ofr = 0.9783. Stability tests also fell within acceptable limits. In this study, we present the validation of the SQ-TLC method and propose its adoption as a rapid screening tool for field estimation of the quality of antimalarial and other essential medicines in Malawi and other parts of the developing world prior to a more accurate HPLC assay.

scholarly journals Infrared Authentication of Ginseng Species: The Use of the 2-6PC Rule

2009 ◽  
Vol 92 (2) ◽  
pp. 672-679 ◽  
Author(s):  
Kevin Yi-Lwern Yap ◽  
Tommy Kok Henglai ◽  
Sui Yung Chan ◽  
Chu Sing Lim
Keyword(s):  
Calcium Oxalate ◽  
Screening Tool ◽  
Chemical Constituents ◽  
Effective Means ◽  
Biologically Active ◽  
Rapid Screening ◽  
Active Components ◽  
Herbal Products ◽  
Consumer Safety

Abstract The quality of herbal products is important for ensuring efficacy and consumer safety. Traditional methods of authenticating herbs like ginseng via their morphology are hardly reliable. Different chemical constituents in herbs like ginseng tend to exhibit characteristic IR fingerprints that enable their identification. We previously introduced an IR-based protocol known as the 2-6PC rule to categorize and identify ginseng and its products, as well as distinguishing it from morphological fakes. Here, we describe the use of this rule as a rapid and effective means of analyzing the IR spectral fingerprints of the biologically active components of ginseng, as well as distinguishing among its species. Our results show that Panax ginseng, P. quinquefolius, and P. notoginseng can be differentiated from each other. Our results also indicate the presence of starch, carbohydrates, calcium oxalate, and ginsenosides Re and Rg1 in commercial ginseng roots sold in Singapore. This work effectively demonstrates the usefulness of the 2-6PC rule as a rapid screening tool in the authentication of ginseng species.

scholarly journals Evaluation of the Quality of Artemisinin-Based Antimalarial Medicines Distributed in Ghana and Togo

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Dorcas Osei-Safo ◽  
Amegnona Agbonon ◽  
Daniel Yeboah Konadu ◽  
Jerry Joe Ebow Kingsley Harrison ◽  
Mamadou Edoh ◽  
...  
Keyword(s):  
Failure Rate ◽  
Quality Evaluation ◽  
Active Pharmaceutical Ingredients ◽  
Hplc Assay ◽  
Pharmaceutical Ingredients ◽  
Cross Border ◽  
Border Towns ◽  
Medicine Quality ◽  
Accurate Quantification

This study, conducted as part of our overall goal of regular pharmacovigilance of antimalarial medicines, reports on the quality of 132 artemisinin-based antimalarial medicines distributed in Ghana and Togo. Three methods were employed in the quality evaluation—basic (colorimetric) tests for establishing the identity of the requisite active pharmaceutical ingredients (APIs), semi-quantitative TLC assay for the identification and estimation of API content, and HPLC assay for a more accurate quantification of API content. From the basic tests, only one sample totally lacked API. The HPLC assay, however, showed that 83.7% of the ACTs and 57.9% of the artemisinin-based monotherapies failed to comply with international pharmacopoeia requirements due to insufficient API content. In most of the ACTs, the artemisinin component was usually the insufficient API. Generally, there was a good correlation between the HPLC and SQ-TLC assays. The overall failure rates for both locally manufactured (77.3%) and imported medicines (77.5%) were comparable. Similarly the unregistered medicines recorded a slightly higher overall failure rate (84.7%) than registered medicines (70.8%). Only two instances of possible cross-border exchange of medicines were observed and there was little difference between the medicine quality of collections from border towns and those from inland parts of both countries.

Pengaruh Kompetensi Coder terhadap Keakuratan dan Ketepatan Pengkodean Menggunakan ICD 10 di Rumah Sakit X Pekanbaru Tahun 2016

2018 ◽  
Vol 1 (1) ◽  
pp. 31-43
Author(s):  
Nur Maimun ◽  
Jihan Natassa ◽  
Wen Via Trisna ◽  
Yeye Supriatin
Keyword(s):  
Medical Record ◽  
Human Error ◽  
Medical Personnel ◽  
Quality Of Data ◽  
Accuracy And Precision ◽  
Result Show ◽  
Study Implementation ◽  
Icd 10 ◽  
The Cost

The accuracy in administering the diagnosis code was the important matter for medical recorder, quality of data was the most important thing for health information management of medical recorder. This study aims to know the coder competency for accuracy and precision of using ICD 10 at X Hospital in Pekanbaru. This study was a qualitative method with case study implementation from five informan. The result show that medical personnel (doctor) have never received a training about coding, doctors writing that hard and difficult to read, failure for making diagnoses code or procedures, doctor used an usual abbreviations that are not standard, theres still an officer who are not understand about the nomenclature and mastering anatomy phatology, facilities and infrastructure were supported for accuracy and precision of the existing code. The errors of coding always happen because there is a human error. The accuracy and precision in coding very influence against the cost of INA CBGs, medical and the committee did most of the work in the case of severity level III, while medical record had a role in monitoring or evaluation of coding implementation. If there are resumes that is not clearly case mix team check file needed medical record the result the diagnoses or coding for conformity. Keywords: coder competency, accuracy and precision of coding, ICD 10

Recent Applications of High Performance Thin Layer Chromatography and Derivative Spectrophotometry in Pharmaceutical Analysis

2020 ◽  
Vol 16 (6) ◽  
pp. 671-689
Author(s):  
Marcin Gackowski ◽  
Marcin Koba ◽  
Katarzyna Mądra-Gackowska ◽  
Piotr Kośliński ◽  
Stefan Kruszewski
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pharmaceutical Analysis ◽  
High Performance ◽  
Degradation Products ◽  
Derivative Spectrophotometry ◽  
Accuracy And Precision ◽  
Post Marketing ◽  
Layer Chromatography

At present, no one can imagine drug development, marketing and post-marketing without rigorous quality control at each stage. Only modern, selective, accurate and precise analytical methods for determination of active compounds, their degradation products and stability studies are able to assure the appropriate amount and purity of drugs administered every day to millions of patients all over the world. For routine control of drugs simple, economic, rapid and reliable methods are desirable. The major focus of current scrutiny is placed on high-performance thin layer chromatography and derivative spectrophotometry methods, which fulfill routine drug estimation’s expectations [1-4]. The present paper reveals state-of-the-art and possible applications of those methods in pharmaceutical analysis between 2010 and 2018. The review shows advantages of high-performance thin layer chromatography and derivative spectrophotometry, including accuracy and precision comparable to more expensive and time-consuming methods as well as additional fields of possible applications, which contribute to resolving many analytical problems in everyday laboratory practice.

scholarly journals Evaluating Alternative Flight Plans in Thermal Drone Wildlife Surveys—Simulation Study

2021 ◽  
Vol 13 (6) ◽  
pp. 1102
Author(s):  
Julia Witczuk ◽  
Stanisław Pagacz
Keyword(s):  
Computer Simulations ◽  
Survey Design ◽  
Distance Sampling ◽  
Line Of Sight ◽  
Density Estimates ◽  
Accuracy And Precision ◽  
Survey Costs ◽  
Random Survey ◽  
Density Population

The rapidly developing technology of unmanned aerial vehicles (drones) extends to the availability of aerial surveys for wildlife research and management. However, regulations limiting drone operations to visual line of sight (VLOS) seriously affect the design of surveys, as flight paths must be concentrated within small sampling blocks. Such a design is inferior to spatially unrestricted randomized designs available if operations beyond visual line of sight (BVLOS) are allowed. We used computer simulations to assess whether the VLOS rule affects the accuracy and precision of wildlife density estimates derived from drone collected data. We tested two alternative flight plans (VLOS vs. BVLOS) in simulated surveys of low-, medium- and high-density populations of a hypothetical ungulate species with three levels of effort (one to three repetitions). The population density was estimated using the ratio estimate and distance sampling method. The observed differences in the accuracy and precision of estimates from the VLOS and BVLOS surveys were relatively small and negligible. Only in the case of the low-density population (2 ind./100 ha) surveyed once was the VLOS design inferior to BVLOS, delivering biased and less precise estimates. These results show that while the VLOS regulations complicate survey logistics and interfere with random survey design, the quality of derived estimates does not have to be compromised. We advise testing alternative survey variants with the aid of computer simulations to achieve reliable estimates while minimizing survey costs.

scholarly journals A rapid screening tool for psychological distress in children 3–6years old: results of a validation study

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Caroline Marquer ◽  
Caroline Barry ◽  
Yoram Mouchenik ◽  
Sarah Hustache ◽  
Douma M Djibo ◽  
...  
Keyword(s):  
Psychological Distress ◽  
Validation Study ◽  
Screening Tool ◽  
Rapid Screening

Assessing pelvic floor quality of life in postpartum women-KHPQ, a novel screening tool

2016 ◽  
Vol 200 ◽  
pp. 128-129
Author(s):  
G. Thiagamoorthy ◽  
D. Robinson ◽  
L. Cardozo ◽  
H. Sekar ◽  
N. Bosompra
Keyword(s):  
Quality Of Life ◽  
Pelvic Floor ◽  
Screening Tool ◽  
Postpartum Women
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