scholarly journals Combined Use of Trisodium Citrate and Transglutaminase to Enhance the Stiffness and Water-Holding Capacity of Acidified Yak Milk Gels

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Lin Yang
Keyword(s):  
Electron Microscopy ◽  
Synergistic Effect ◽  
Skim Milk ◽  
Textural Properties ◽  
Trisodium Citrate ◽  
Water Holding Capacity ◽  
Cross Linking ◽  
Yak Milk ◽  
Combined Use ◽  
Water Holding

In this research, the synergistic effect of trisodium citrate (TSC) and microbial transglutaminase (TGase) treatment on the textural properties of acidified yak skim milk gels was investigated. TSC was added to yak skim milk to concentrations of 0, 20, and 40 mmol/L, followed by adjusting the pH to 6.7. The samples were incubated with TGase for the cross-linking reaction, after which the samples were acidified with 1.4% (w/v) gluconodelta-lactone (GDL) at 42°C for 4 h to form gels. The stiffness and water holding capacity (WHC) of gels exhibited higher values at 20 or 40 mmol/L than without TSC. The final storage modulus (G′) of yak milk gels was positively related to the concentration of TSC prior to TGase treatment. Cryoscanning electron microscopy observations showed that the gel networks were more rigid with higher TSC concentrations. Overall, TSC dissociated particles in yak milk into smaller ones. The newly formed particles in yak skim milk could form acid-induced gels with greater stiffness and higher WHC in the presence of TGase.

scholarly journals Synergistic Effect of Heating pH and Transglutaminase on the Gelation Kinetics and Texture of Yak Skim Milk Gels

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Lin Yang ◽  
Zhaomin Li ◽  
Xuedong Gu ◽  
Fumin Chi ◽  
Yahui Zhu
Keyword(s):  
Ph Value ◽  
Synergistic Effects ◽  
Gelation Time ◽  
Skim Milk ◽  
Textural Properties ◽  
Yak Milk ◽  
Ph Values ◽  
Gelation Kinetics ◽  
Opposite Trend ◽  
Water Holding

Textural defects (including syneresis and poor consistency) often occur in yogurt gels produced from yak milk. In this research, the synergistic effects of transglutaminase (TGase) and heating pH on the textural properties of acidified yak skim milk gels, as well as the related mechanism of action, were investigated. The pH values of yak skim milk were adjusted to 6.3, 6.7, and 7.1, respectively. The samples were heated at 80°C for 30 min and then cooled to 42°C. After treatment with different contents of TGase (0, 3, and 10 U TGase per gram proteins), the samples were acidified with glucono-delta-lactone. For a given TGase content, the final storage modulus (G′) of gels was positively related to the heating pH, whereas the opposite trend was observed for the gelation time. This effect was obvious between pH 6.3 and 6.7. At a definite heating pH value, the final G′ of the gels was highest at 3 U TGase per gram proteins. The highest water holding capacity and firmness value were observed in gels prepared using pH 7.1 and 3 U TGase per gram proteins. In the samples treated with 3 U TGase per gram proteins (preheating pH 7.1), more rigid network structures were seen in the gel than 0 or 10 U TGase per gram proteins. Therefore, adjusting the heating pH values and TGase contents is an effective way of improving the textural properties of yak milk gels.

POTENTIAL USE OF OKARA AS MEAT REPLACER IN BEEF SAUSAGE

2016 ◽  
Vol 78 (6-6) ◽  
Author(s):  
Noriham, A. ◽  
Muhammad Ariffaizuddin, R. ◽  
Noorlaila, A. ◽  
Faris Zakry, A. N
Keyword(s):  
Salt Content ◽  
Meat Products ◽  
Textural Properties ◽  
Water Holding Capacity ◽  
Processed Meat ◽  
Color Analysis ◽  
Overall Acceptability ◽  
A Value ◽  
Water Holding ◽  
Potential Use

Processed meat products are particularly unhealthy because of high fat, preservative and salt content. This study is carried out with the aim to determine the physicochemical and sensorial properties of sausage incorporated with okara flour. There were four different sausage formulations labelled as Control (0% okara flour, 100% beef), F1 (10% okara flour, 90% beef), F2 (20% okara flour, 80% beef), F3 (30% okara flour, 70% beef) and F4 (40% okara flour, 60% beef). Formulations were subjected to proximate, water holding capacity, color, texture and sensorial analysis. Results for proximate composition, revealed that carbohydrate, ash and fiber content increased while moisture, fat and protein content decreased as the okara flour addition were increased. Water holding capacity (WHC) was found to increase as the incorporation of okara flour increased. In term of color analysis, increased in okara flour content in sausage significantly increased lightness (L*) and yellowness (b*) values while decreasing in redness (a*) value. As for textural properties, the values for hardness, cohesiveness, springiness and chewiness were decreased as the incorporation of okara flour increased. Sensorial results showed that F4 had the lowest overall acceptability due to its poor texture and unacceptable taste. Hence this study concludes that okara flour has the potential to replace meat at certain levels in sausage formulations which is not more than 20% okara flour.

scholarly journals Preparation and sensory evaluation of functional drink based on papaya (Carica papaya L.) pulp

2019 ◽  
Vol 17 (3) ◽  
pp. 388-395
Author(s):  
Nobel Dev ◽  
Md. Sajjad Hossain ◽  
Abdullah Iqbal
Keyword(s):  
Moisture Content ◽  
Milk Powder ◽  
Skim Milk ◽  
Egg White ◽  
Skim Milk Powder ◽  
Sensory Properties ◽  
Water Holding Capacity ◽  
Drying Time ◽  
Functional Drink ◽  
Water Holding

This research was aimed to develop functional drink based on foam-mat drying of papaya. Papaya pulp was foamed by the addition of 10, 15 and 20% egg white (w/w). Foamed papaya slurry was dried by using hot air at 60 0C for 5 hours. Drying yield and protein content of dried powder increased with the increase of egg white. Vitamin C decreased in powder due to heat degradation. Higher foam thickness resulted in longer drying time. Drying time of foamed pulp was much lower than the non-foamed pulp. Moisture content, reconstitution and water holding capacity of foam-mat dried powder were in the range of 3.27-3.93%, 82.43-83.53% and 5.23-5.93 g/g, respectively. Functional drinks were formulated by dry blending of foam-mat dried papaya powder and other ingredients at the ratio of 6:4, 5:5 and 4:6 (w/w) of foam-mat dried papaya powder and skim milk. Different ratio of foam-mat dried papaya and skim milk powder affected the physicochemical properties (moisture content, reconstitution rate, and water holding capacity) and sensory properties (color, viscosity, flavor and taste) of the product. The reconstituted drink at a ratio of 4:6 of foam-mat dried papaya powder and skim milk retained the best sensory properties. J Bangladesh Agril Univ 17(3): 388–395, 2019

scholarly journals Physico-chemical properties of probiotic yoghurt produced with transglutaminase

2007 ◽  
pp. 45-52 ◽  
Author(s):  
Spasenija Milanovic ◽  
Marijana Caric ◽  
Mirjana Djuric ◽  
Mirela Ilicic ◽  
Katarina Durakovic
Keyword(s):  
Chemical Composition ◽  
Starter Culture ◽  
Skim Milk ◽  
Chemical Properties ◽  
Enzyme Activation ◽  
Water Holding Capacity ◽  
Laboratory Scale ◽  
Sensory Characteristics ◽  
Physico Chemical ◽  
Water Holding

The effect of different concentration of transglutaminase - TG (0.02%, 0.06% and 0.12%) on physico-chemical properties of probiotic yoghurt was investigated. Two series of yoghurt were manufactured on a laboratory scale from pasteurised skim milk (0.1 % w/w fat). Series I was produced with TGase activation during a period of 2 h at 40?C, while series II was produced without enzyme activation. Then, the adequate quantities of TGase and probiotic starter culture ABT-4 (Chr. Hansen A/S Denmark) were added. Chemical composition, physico-chemical properties (water holding capacity and whey separation) and sensory characteristics of yoghurt samples were determined after production and during 5 days of storage. Addition of TGase to milk (direct or after activation) for probiotic yoghurt manufacture improved its overall characteristics. Activation of TG in yoghurt production increases water holding capacity as well as decreases syneresis during the storage.

scholarly journals Modifikasi Sifat Fisik Yogurt Susu Kambing dengan Penambahan Microbial Transglutaminase dan Sumber Protein Eksternal

2020 ◽  
Vol 9 (2) ◽  
pp. 77-82
Author(s):  
Salvian Setyo Prayitno ◽  
Juni Sumarmono ◽  
Agustinus Hantoro Djoko Rahardjo ◽  
Triana Setyawardani
Keyword(s):  
Physical Properties ◽  
Whey Protein ◽  
Skim Milk ◽  
Goat Milk ◽  
Microbial Transglutaminase ◽  
Water Holding Capacity ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Protein Sources ◽  
Water Holding

Penelitian ini bertujuan untuk mempelajari sifat fisik yogurt susu kambing yang dimodifikasi dengan enzim mTGase dan sumber protein eksternal. Sifat fisik yang diamati meliputi sineresis spontan (wheying-off), sineresis, water holding capacity (WHC) dan viskositas. Materi yang digunakan yaitu susu segar kambing etawah, kultur starter yogurt, enzim mTGase, susu skim bubuk, dan whey protein concentrate (WPC). Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan dan 5 kali ulangan. Perlakuan terdiri atas kontrol yaitu susu kambing segar, penambahan mTGase sebanyak 0,03% (w/w), mTGase dan susu skim 1% (w/w), mTGase dan whey protein concentrate 1% (w/w). Susu dikondisikan selama 24 jam pada refrigerator (10˚C) sebelum difermentasi menjadi yogurt. Parameter yang diuji berupa wheying-off, sineresis, water holding capacity, dan viskositas yang diukur 1 jam setelah yogurt dikeluarkan dari refrigerator. Hasil penelitian menunjukkan bahwa enzim mTGase secara signifikan menyebabkan penurunan sineresis, peningkatan WHC, dan viskositas, namun tidak menyebabkan perbedaan yang signifikan pada wheying-off yogurt. Kombinasi mTGase dan sumber protein eksternal menurunkan sineresis secara signifikan, namun tidak berpengaruh signifikan terhadap wheying-off, WHC, dan viskositas yogurt. Kombinasi mTGase + WPC 1% menghasilkan kualitas fisik yogurt yang tidak jauh berbeda dengan kombinasi mTGase + skim 1% tehadap semua parameter yang diukur. Berdasarkan hasil penelitian, dapat disimpulkan bahwa sifat fisik yogurt susu sapi dapat dimodifikasi dengan enzim mTGase saja atau kombinasi dengan sumber protein eksternal. Manfaat penelitian ini adalah memberikan informasi penggunaan enzim mTGase yang dikombinasikan dengan WPC atau susu skim 1% ternyata dapat meningkatkan kualitas fisik yogurt. Modification of Physical Properties of Goat Milk Yogurt by Addition of Microbial Transglutaminase Enzyme and External Protein SourcesAbstractThe purpose of this research was to study the modification of the physical properties of goat milk yogurt with the addition of the enzyme transglutaminase (mTGase) and external protein. The benefit of this research was to provide information on methods to improve the quality of yogurt in terms of the physical properties of yogurt. The research used fresh goat milk, dry starter culture, mTGase enzyme, skimmed milk powder, and whey protein concentrate (WPC). A completely randomized design with 4 treatments and 5 replications was used as research design. The treatments were fresh goat milk as control, fresh goat milk with 0.03% w/w mTGase, mTGase and 1% w/w skim milk, mTGase and 1% w/w whey protein concentrate. The milk was stored for 24 hours in a refrigerator (10˚C) prior to fermentation process. Wheying-off, syneresis, water holding capacity and viscosity were then measured at an hour after yogurt was removed from the refrigerator. The results showed that mTGase significantly reduced syneresis, increased WHC, and viscosity, but had no significant effect on wheying-off. The combination of mTGase + external protein sources significantly reduced syneresis, but the effect on wheying-off, WHC and yogurt were not significantly detected. The combination of mTGase + 1% WPC had similar characteristics as mTGase + 1% skim milk. In conclusion, the physical characteristics of yogurt from goat milk could be modified by mTGase enzyme or in combination with external protein sources. The use of mTGase enzyme in combination with WPC or skim milk improves the physical characteristics of yogurt. 

scholarly journals Improvement of physicochemical and rheological properties of kombucha fermented milk products by addition of transglutaminase and whey protein concentrate

2016 ◽  
pp. 11-18 ◽  
Author(s):  
Mirela Ilicic ◽  
Spasenija Milanovic ◽  
Katarina Kanuric ◽  
Vladimir Vukic ◽  
Dajana Vukic
Keyword(s):  
Rheological Properties ◽  
Whey Protein ◽  
Skim Milk ◽  
Fermented Milk ◽  
Water Holding Capacity ◽  
Whey Protein Concentrate ◽  
Milk Product ◽  
Protein Concentrate ◽  
Water Holding

The objective of this work was to investigate the effect of addition of transglutaminase (TG-0.02%, w/w) and whey protein concentrate (WPC-0.03%, w/w), on quality of kombucha fermented milk product. Samples were prepared from pasteurized semi-skim milk (0.9%, w/w fat) and kombucha inoculum (10%, v/v). The pH values were measured during the fermentation of milk (lasted until reached 4.5). Syneresis, water holding capacity and the product texture (firmness and consistency,) were assessed after production. Rheological properties of kombucha fermented milk samples were measured during ten days of storage. The sample containing TG had the lowest syneresis (21 ml), the highest water holding capacity (62%) and the highest textural characteristics (firmness - 23.99g, consistency - 626.54gs) after production. The addition of WPC to milk improved the rheological properties, while the addition of TG improved it even to a significantly greater extent after the production and during 10 days of the storage.

scholarly journals Quality of Plain Set Yoghurt as affected by Levels of Ultrafiltration Concentration of Milk and Inoculum of Yoghurt Culture

2016 ◽  
Vol 4 (6) ◽  
pp. 508 ◽  
Author(s):  
Narayana Mudiyanselage Nayana Kumari Narayana ◽  
Vijay Kumar Gupta
Keyword(s):  
Concentration Level ◽  
Skim Milk ◽  
Titratable Acidity ◽  
Streptococcus Thermophilus ◽  
Water Holding Capacity ◽  
Lactobacillus Delbrueckii ◽  
Inoculum Level ◽  
Overall Acceptability ◽  
Water Holding

Quality of plain set yoghurt was investigated by varying levels of ultrafiltration concentration of cow skim milk (1-without ultrafiltration, 1.5 and 2 folds) and inoculum (2, 2.5 and 3%) of yoghurt culture (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus 1:1). Titratable acidity development and pH reduction were significantly faster during incubation of yoghurt with increasing ultrafiltration concentration level and inoculum level. Increased ultrafiltration concentration level decreased spontaneous whey syneresis, but increased water holding capacity and firmness of the product significantly. Body & texture and overall acceptability scores were significantly better in yoghurts prepared from 1.5 fold concentrated milk irrespective of the inoculum level studied. Inoculum level did not show any significant effect on the quality of yoghurt, but higher inoculum level decreased the incubation period significantly. Optimized product was obtained with 1.5 fold concentrated skim milk standardized to 3.3% fat and incubated with 2% inoculum level in about five and a half hour of incubation. Optimized product had 13.60±0.02% total solids, 3.31±0.01% fat, 5.27±0.04% protein, 4.20±0.03% lactose and 0.82±0.02% ash. Whey syneresis, water holding capacity and firmness in optimized product were nil, 63.49% and 1.89 N, respectively. Thus, high quality plain yoghurt could be prepared by employing ultrafiltration, without addition of stabilizers, which is otherwise widely used commercially for the manufacture of yoghurt to control its wheying off and body.

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