scholarly journals Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Lucía Arévalo-Híjar ◽  
Miguel Ángel Aguilar-Luis ◽  
Stefany Caballero-García ◽  
Néstor Gonzáles-Soto ◽  
Juana Del Valle-Mendoza
Keyword(s):  
Enterococcus Faecalis ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Inhibition Zone ◽  
Bactericidal Effect ◽  
Growth Inhibition Zone ◽  
Methanolic Extracts

Objective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results. The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions. In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.

scholarly journals EFEK ANTI JAMUR AIR RENDAMAN DAUN KELOR (Moringa oleifera) TERHADAP PERTUMBUHAN JAMUR Trichophyton mentagrophytes SECARA IN VITRO

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Jirna Inyoman
Keyword(s):  
Moringa Oleifera ◽  
Antimicrobial Properties ◽  
Test Group ◽  
Average Diameter ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Growth Inhibition Zone ◽  
Post Test ◽  
Test Use

Abstract Background Moringa oleifera is a plant that all parts can be used for medicinal purposes. Roots, flowers, bark, stems, leaves and seeds of Moringa has antimicrobial properties. Compound content in leaves of moringa, such as tannin,steroid, alkaloid, saponins ,and flavonoids . ObjectiveThe purpose of this research was to know the different of Trichophyton mentagrophytes growth on various leaves of moringa (Moringa oleifera) concentration extract. Methods This research uses a true experimental with post test only kontrol group design. The antifungal activity test use diffusion disk method, these were divided into four test group with concentration 25%, 50%, 75%, 100% and two kontrol group were positive kontrol (ketokonazole 2%) and negatif kontrol (sterile distilled water). The result of this study showed that the average diameter of inhibition zone of concentration 25% until 100% is 0 mm. Statistic analysis was done by using Kolmogorof Smirnov test and Kruskal Wallis test. The test showed there was not a significant mean difference between group. All consentration haven’t an effect on Trichophyton mentagrophytes growth inhibition zone diameter. For next research with same topics using pure extract leaves of moringa was recommended. Keywords: Inhibition zone, Moringa oleifera, Trichophyton mentagrophytes.

scholarly journals Antibacterial Effect of Hydroalcoholic Extract ofPunica granatumLinn. Petal on Common Oral Microorganisms

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Farnaz Hajifattahi ◽  
Elham Moravej-Salehi ◽  
Maryam Taheri ◽  
Arash Mahboubi ◽  
Mohammad Kamalinejad
Keyword(s):  
Growth Inhibition ◽  
Antibacterial Effect ◽  
Brain Heart Infusion ◽  
Inhibition Zone ◽  
Maximum Effect ◽  
Streptococcus Sobrinus ◽  
Bacterial Strains ◽  
Hydroalcoholic Extract ◽  
Growth Inhibition Zone

Objectives. This study aimed to assess the effect of hydroalcoholic extract ofPunica granatumLinn. (P. granatum) petal onStreptococcus sanguinis,Streptococcus mutans,Streptococcus salivarius,Streptococcus sobrinus,andEnterococcus faecalis.Materials and Methods. In this in vitro study,P. granatumextract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX), and ampicillin was evaluated on brain heart infusion agar (BHIA) using the cup-plate method. By assessing the diameter of the growth inhibition zone, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were determined for the above-mentioned bacteria.Results. Hydroalcoholic extract ofP. granatumpetal had inhibitory effects on the proliferation of all five bacterial strains with maximum effect onS. mutanswith MIC and MBC of 3.9 mg/mL. The largest growth inhibition zone diameter belonged toS. sanguinisand the smallest toE. faecalis. Ampicillin and CHX had the greatest inhibitory effect onS. sanguinis.Conclusions. Hydroalcoholic extract ofP. granatumhad a significant antibacterial effect on common oral bacterial pathogens with maximum effect onS. mutans, which is the main microorganism responsible for dental plaque and caries.

Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Olubukola H. Oyeniran ◽  
Adedayo O. Ademiluyi ◽  
Ganiyu Oboh
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Methanolic Extract ◽  
Array Detector ◽  
Oh Radicals ◽  
Inhibitory Effects ◽  
Methanolic Extracts ◽  
Phenolic Constituents ◽  
Rauvolfia Vomitoria

AbstractObjectivesRauvolfia vomitoria is a medicinal plant used traditionally in Africa in the management of several human diseases including psychosis. However, there is inadequate scientific information on the potency of the phenolic constituents of R. vomitoria leaf in the management of neurodegeneration. Therefore, this study characterized the phenolic constituents and investigated the effects of aqueous and methanolic extracts of R. vomitoria leaf on free radicals, Fe2+-induced lipid peroxidation, and critical enzymes linked to neurodegeneration in rat’s brain in vitro.MethodsThe polyphenols were evaluated by characterizing phenolic constituents using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). The antioxidant properties were assessed through the extracts ability to reduce Fe3+ to Fe2+; inhibit ABTS, DPPH, and OH radicals and Fe2+-induced lipid peroxidation. The effects of the extracts on AChE and MAO were also evaluated.ResultsThe phenolic characterization of R. vomitoria leaf revealed that there were more flavonoids present. Both aqueous and methanolic extracts of R. vomitoria leaf had inhibitory effects with the methanolic extract having higher significant (p≤0.05) free radicals scavenging ability coupled with inhibition of monoamine oxidases. However, there was no significant (p≤0.05) difference obtained in the inhibition of lipid peroxidation and cholinesterases.ConclusionThis study suggests that the rich phenolic constituents of R. vomitoria leaf might contribute to the observed antioxidative and neuroprotective effects. The methanolic extract was more potent than the aqueous extract; therefore, extraction of R. vomitoria leaf with methanol could offer better health-promoting effects in neurodegenerative condition.

Evaluation of the antibacterial effect of tea tree oil on Enterococcus faecalis and biofilm in vitro

2021 ◽  
pp. 114566
Author(s):  
Jianyan Qi ◽  
Min Gong ◽  
Rui Zhang ◽  
Yumeng Song ◽  
Qian Liu ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Tea Tree Oil ◽  
Tea Tree

Antibacterial Effect of Two Mineral Trioxide Aggregate (MTA) Preparations Against Enterococcus faecalis and Streptococcus sanguis In Vitro

2006 ◽  
Vol 32 (11) ◽  
pp. 1053-1056 ◽  
Author(s):  
Khalid Al-Hezaimi ◽  
Thakib A. Al-Shalan ◽  
Jafar Naghshbandi ◽  
Samuel Oglesby ◽  
James H.S. Simon ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Mineral Trioxide Aggregate ◽  
Streptococcus Sanguis

scholarly journals Mammalian Arginase Inhibitory Activity of Methanolic Extracts and Isolated Compounds from Cyperus Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1694
Author(s):  
Kamel Arraki ◽  
Perle Totoson ◽  
Alain Decendit ◽  
Andy Zedet ◽  
Justine Maroilley ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Isolation And Identification ◽  
Vasorelaxant Effect ◽  
Methanolic Extracts ◽  
Phytochemical Investigation ◽  
Ic50 Values ◽  
First Time

Polyphenolic enriched extracts from two species of Cyperus, Cyperus glomeratus and Cyperus thunbergii, possess mammalian arginase inhibitory capacities, with the percentage inhibition ranging from 80% to 95% at 100 µg/mL and 40% to 64% at 10 µg/mL. Phytochemical investigation of these species led to the isolation and identification of two new natural stilbene oligomers named thunbergin A-B (1–2), together with three other stilbenes, trans-resveratrol (3), trans-scirpusin A (4), trans-cyperusphenol A (6), and two flavonoids, aureusidin (5) and luteolin (7), which were isolated for the first time from C.thunbergii and C. glomeratus. Structures were established on the basis of the spectroscopic data from MS and NMR experiments. The arginase inhibitory activity of compounds 1–7 was evaluated through an in vitro arginase inhibitory assay using purified liver bovine arginase. As a result, five compounds (1, 4–7) showed significant inhibition of arginase, with IC50 values between 17.6 and 60.6 µM, in the range of those of the natural arginase inhibitor piceatannol (12.6 µM). In addition, methanolic extract from Cyperus thunbergii exhibited an endothelium and NO-dependent vasorelaxant effect on thoracic aortic rings from rats and improved endothelial dysfunction in an adjuvant-induced arthritis rat model.

New manufacturing process to develop antibacterial dyed polyethylene terephthalate sutures using plasma functionalization and chitosan immobilization

2021 ◽  
pp. 152808372110505
Author(s):  
Nesrine Bhouri ◽  
Faten Debbabi ◽  
Abderrahmen Merghni ◽  
Esther Rohleder ◽  
Boris Mahltig ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Terephthalate ◽  
Manufacturing Process ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
The United States ◽  
Mechanical Tests ◽  
Inflammatory Reactions ◽  
Plasma Functionalization

The main purpose of this paper is to develop a new manufacturing process leading to have antibacterial dyed non-absorbable braided polyethylene terephthalate (PET) sutures using biocompatible and non-toxic products. This manufacturing process allows better visibility of sutures in the surgical field and reduces the risk of infections and inflammatory reactions without affecting the mechanical properties while meeting the United States Pharmacopeia (USP) requirements. Plasma functionalization, acrylic acid (AA) grafting, and bioactive chitosan (CH) coating were used before the dyeing process with a biocompatible non-toxic acid dye, approved by the Food and Drug Administration (FDA). The influence of experimental parameters on the suture properties and the K/S values of the dyed sutures are investigated. Infrared spectroscopy confirms the presence of new bonds to immobilize chitosan on the surface of the suture. Mechanical tests confirm that the mechanical properties of sutures have not been affected. The in vitro antibacterial effect of dyed PET sutures showed an inhibition zone of 11 mm against S. aureus, 4 mm against P. aeruginosa, and 1 mm against E. coli. This study reveals that the new finishing process of sutures is a promising method to achieve an antibacterial effect with a uniform shade and smooth surfaces.

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