scholarly journals Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Zhiwei Jiang ◽  
Yue Xi ◽  
Kaichen Lai ◽  
Ying Wang ◽  
Huiming Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Sheet Thickness ◽  
Collagen Type I ◽  
Type I ◽  
Cell Sheets ◽  
Cell Sheet Technology ◽  
Rat Bone

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

scholarly journals Designing 3D Mesenchymal Stem Cell Sheets Merging Magnetic and Fluorescent Features: When Cell Sheet Technology Meets Image-Guided Cell Therapy

Theranostics ◽  
2016 ◽  
Vol 6 (5) ◽  
pp. 739-751 ◽  
Author(s):  
Gabriel Rahmi ◽  
Laetitia Pidial ◽  
Amanda K. A. Silva ◽  
Eléonore Blondiaux ◽  
Bertrand Meresse ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Image Guided ◽  
Cell Sheet Technology

scholarly journals Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Mitsuyoshi Nakao ◽  
Kyungsook Kim ◽  
Kenichi Nagase ◽  
David W. Grainger ◽  
Hideko Kanazawa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Cellular Structures ◽  
Phenotypic Traits ◽  
Cell Sheets ◽  
Temperature Responsive ◽  
Cell Sheet Technology ◽  
Responsive Cell

Abstract Background In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inconsistent, largely attributed to transplanted MSC difficulties both in engrafting at tissue sites and in retaining their therapeutic functions from suspension formulations. MSC functional components, including cell adhesion and cell–cell junction proteins, and ECM that contribute to essential cellular therapeutic effects, are damaged or removed by proteolytic enzymes used in stem cell harvesting strategies from culture. To overcome these limitations, methods to harvest and transplant cells without disrupting critical stem cell functions are required. Cell sheet technology, exploiting temperature-responsive cell culture surfaces, permits cell harvest without cell protein damage. This study is focused on phenotypic traits of MSC sheets structurally and functionally to understand therapeutic benefits of cell sheets. Methods/results This study verified cleaved cellular proteins (vinculin, fibronectin, laminin, integrin β-1, and connexin 43) and increased apoptotic cell death produced under standard trypsin harvesting treatment in a time-dependent manner. However, MSC sheets produced without trypsin using only temperature-controlled sheet harvest from culture plastic exhibited intact cellular structures. Also, MSCs harvested using enzymatic treatment (i.e., chemical disruption) showed higher pYAP expression compared to MSC sheets. Conclusion Retention of cellular structures such as ECM, cell–cell junctions, and cell–ECM junctions is correlated with human umbilical cord mesenchymal stem cell (hUC-MSC) survival after detachment from cell culture surfaces. Retaining these proteins intact in MSC cultures using cell sheet technology is proposed to enhance stem cell survival and their function in stem cell-based therapy.

scholarly journals Nicotinamide phosphoribosyltransferase postpones rat bone marrow mesenchymal stem cell senescence by mediating NAD+–Sirt1 signaling

Aging ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 3505-3522 ◽  
Author(s):  
Chenchen Pi ◽  
Yue Yang ◽  
Yanan Sun ◽  
Huan Wang ◽  
Hui Sun ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Senescence ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Rat Bone

scholarly journals Suppression of Carbon Tetrachloride-Induced Liver Fibrosis by Transplantation of a Clonal Mesenchymal Stem Cell Line Derived from Rat Bone Marrow

2009 ◽  
Vol 18 (1) ◽  
pp. 89-100 ◽  
Author(s):  
Marhaen Hardjo ◽  
Masahiro Miyazaki ◽  
Masakiyo Sakaguchi ◽  
Takuro Masaka ◽  
Sukaeni Ibrahim ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Mesenchymal Stem Cell ◽  
Cell Line ◽  
Stem Cell Line ◽  
Rat Bone

The performance of bone marrow mesenchymal stem cell – Implant complexes prepared by cell sheet engineering techniques

Biomaterials ◽  
2010 ◽  
Vol 31 (12) ◽  
pp. 3212-3221 ◽  
Author(s):  
Wei Zhou ◽  
Chun Han ◽  
Yingliang Song ◽  
Xingrong Yan ◽  
Dehua Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Cell Sheet Engineering

scholarly journals Preparation of high bioactivity multilayered bone-marrow mesenchymal stem cell sheets for myocardial infarction using a 3D-dynamic system

2018 ◽  
Vol 72 ◽  
pp. 182-195 ◽  
Author(s):  
Yingwei Wang ◽  
Jianhua Zhang ◽  
Zixi Qin ◽  
Zepei Fan ◽  
Cheng Lu ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Dynamic System ◽  
Cell Sheets
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