scholarly journals Analytical Strategies Involved in the Detailed Componential Characterization of Biooil Produced from Lignocellulosic Biomass

2017 ◽  
Vol 2017 ◽  
pp. 1-19 ◽  
Author(s):  
Yao Lu ◽  
Guo-Sheng Li ◽  
Yong-Chao Lu ◽  
Xing Fan ◽  
Xian-Yong Wei
Keyword(s):  
Mass Spectrometry ◽  
Lignocellulosic Biomass ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Value Added ◽  
Organic Species ◽  
Analytical Strategies ◽  
Main Components ◽  
Heteroatomic Organic Compounds

Elucidation of chemical composition of biooil is essentially important to evaluate the process of lignocellulosic biomass (LCBM) conversion and its upgrading and suggest proper value-added utilization like producing fuel and feedstock for fine chemicals. Although the main components of LCBM are cellulose, hemicelluloses, and lignin, the chemicals derived from LCBM differ significantly due to the various feedstock and methods used for the decomposition. Biooil, produced from pyrolysis of LCBM, contains hundreds of organic chemicals with various classes. This review covers the methodologies used for the componential analysis of biooil, including pretreatments and instrumental analysis techniques. The use of chromatographic and spectrometric methods was highlighted, covering the conventional techniques such as gas chromatography, high performance liquid chromatography, Fourier transform infrared spectroscopy, nuclear magnetic resonance, and mass spectrometry. The combination of preseparation methods and instrumental technologies is a robust pathway for the detailed componential characterization of biooil. The organic species in biooils can be classified into alkanes, alkenes, alkynes, benzene-ring containing hydrocarbons, ethers, alcohols, phenols, aldehydes, ketones, esters, carboxylic acids, and other heteroatomic organic compounds. The recent development of high resolution mass spectrometry and multidimensional hyphenated chromatographic and spectrometric techniques has considerably elucidated the composition of biooils.

scholarly journals High performance liquid chromatography coupled with high resolution mass spectrometry-based characterization of lipidomic responses from rats with kidney injuries

RSC Advances ◽  
2018 ◽  
Vol 8 (36) ◽  
pp. 20250-20258
Author(s):  
Qun Liang ◽  
Han Liu ◽  
Xiuli Li ◽  
Yang Zhang
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lipid Metabolism ◽  
High Resolution ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Resolution Mass

In this study, we present targeted and non-targeted lipidomics strategies to discover the lipid metabolism variation in serum in rats with sepsis-induced kidney injuries.

scholarly journals VDACs Post-Translational Modifications Discovery by Mass Spectrometry: Impact on Their Hub Function

2021 ◽  
Vol 22 (23) ◽  
pp. 12833
Author(s):  
Maria Gaetana Giovanna Pittalà ◽  
Stefano Conti Nibali ◽  
Simona Reina ◽  
Vincenzo Cunsolo ◽  
Antonella Di Francesco ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Characterization ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Complex Mixture ◽  
Reversed Phase ◽  
Vital Role ◽  
Membrane Pore ◽  
Post Translational Modifications

VDAC (voltage-dependent anion selective channel) proteins, also known as mitochondrial porins, are the most abundant proteins of the outer mitochondrial membrane (OMM), where they play a vital role in various cellular processes, in the regulation of metabolism, and in survival pathways. There is increasing consensus about their function as a cellular hub, connecting bioenergetics functions to the rest of the cell. The structural characterization of VDACs presents challenging issues due to their very high hydrophobicity, low solubility, the difficulty to separate them from other mitochondrial proteins of similar hydrophobicity and the practical impossibility to isolate each single isoform. Consequently, it is necessary to analyze them as components of a relatively complex mixture. Due to the experimental difficulties in their structural characterization, post-translational modifications (PTMs) of VDAC proteins represent a little explored field. Only in recent years, the increasing number of tools aimed at identifying and quantifying PTMs has allowed to increase our knowledge in this field and in the mechanisms that regulate functions and interactions of mitochondrial porins. In particular, the development of nano-reversed phase ultra-high performance liquid chromatography (nanoRP-UHPLC) and ultra-sensitive high-resolution mass spectrometry (HRMS) methods has played a key role in this field. The findings obtained on VDAC PTMs using such methodologies, which permitted an in-depth characterization of these very hydrophobic trans-membrane pore proteins, are summarized in this review.

scholarly journals Retraction: High performance liquid chromatography coupled with high resolution mass spectrometry-based characterization of lipidomic responses from rats with kidney injuries

RSC Advances ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 6234-6234
Author(s):  
Laura Fisher
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Resolution Mass

Retraction of ‘High performance liquid chromatography coupled with high resolution mass spectrometry-based characterization of lipidomic responses from rats with kidney injuries’ by Qun Liang et al., RSC Adv., 2018, 8, 20250–20258, DOI: 10.1039/C8RA02805B.

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