scholarly journals Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Katsunori Shiraishi ◽  
Naosuke Kamei ◽  
Shunsuke Takeuchi ◽  
Shinobu Yanada ◽  
Hisashi Mera ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mrna Expression ◽  
Cartilage Repair ◽  
Expression Profiles ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Cartilage Differentiation ◽  
And Cartilage

Quality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs (miRNAs) were analyzed in human bone marrow MSCs from five different donors. We investigated the correlations of these expression profiles with the capacity of the MSCs for proliferation, chondrogenic differentiation, and cartilage repair in vivo. The mRNA expression of MYBL1 was positively correlated with proliferation and cartilage differentiation. By contrast, the mRNA expression of RCAN2 and the protein expression of TIMP-1 and VEGF were negatively correlated with proliferation and cartilage differentiation. However, MSCs from all five donors had the capacity to promote cartilage repair in vivo regardless of their capacity for proliferation and cartilage differentiation. The mRNA expression of HLA-DRB1 was positively correlated with cartilage repair in vivo. Meanwhile, the mRNA expression of TMEM155 and expression of miR-486-3p, miR-148b, miR-93, and miR-320B were negatively correlated with cartilage repair. The expression analysis of these factors might help to predict the ability of bone marrow MSCs to promote cartilage repair.

scholarly journals Exosome-transported circRNA_0001236 enhances chondrogenesis and suppress cartilage degradation via the miR-3677-3p/Sox9 axis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guping Mao ◽  
Yiyang Xu ◽  
Dianbo Long ◽  
Hong Sun ◽  
Hongyi Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Model ◽  
Chondrogenic Differentiation ◽  
Cartilage Degradation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Luciferase Reporter ◽  
Gene And Protein Expression

Abstract Objectives Aberrations in exosomal circular RNA (circRNA) expression have been identified in various human diseases. In this study, we investigated whether exosomal circRNAs could act as competing endogenous RNAs (ceRNAs) to regulate the pathological process of osteoarthritis (OA). This study aimed to elucidate the specific MSC-derived exosomal circRNAs responsible for MSC-mediated chondrogenic differentiation using human bone marrow-derived MSCs (hMSCs) and a destabilization of the medial meniscus (DMM) mouse model of OA. Methods Exosomal circRNA deep sequencing was performed to evaluate the expression of circRNAs in human bone marrow-derived MSCs (hMSCs) induced to undergo chondrogenesis from day 0 to day 21. The regulatory and functional roles of exosomal circRNA_0001236 were examined on day 21 after inducing chondrogenesis in hMSCs and were validated in vitro and in vivo. The downstream target of circRNA_0001236 was also explored in vitro and in vivo using bioinformatics analyses. A luciferase reporter assay was used to evaluate the interaction between circRNA_0001236 and miR-3677-3p as well as the target gene sex-determining region Y-box 9 (Sox9). The function and mechanism of exosomal circRNA_0001236 in OA were explored in the DMM mouse model. Results Upregulation of exosomal circRNA_0001236 enhanced the expression of Col2a1 and Sox9 but inhibited that of MMP13 in hMSCs induced to undergo chondrogenesis. Moreover, circRNA_0001236 acted as an miR-3677-3p sponge and functioned in human chondrocytes via targeting miR-3677-3p and Sox9. Intra-articular injection of exosomal circRNA_0001236 attenuated OA in the DMM mouse model. Conclusions Our results reveal an important role for a novel exosomal circRNA_0001236 in chondrogenic differentiation. Overexpression of exosomal circRNA_0001236 promoted cartilage-specific gene and protein expression through the miR-3677-3p/Sox9 axis. Thus, circRNA_0001236-overexpressing exosomes may alleviate cartilage degradation, suppressing OA progression and enhancing cartilage repair. Our findings provide a potentially effective therapeutic strategy for treating OA.

scholarly journals The cytokinetic and cytotoxic effects of ICRF-159 and ICRF-187 in vitro and ICRF-187 in human bone marrow in vivo

1983 ◽  
Vol 1 (4) ◽  
Author(s):  
RichardH. Wheeler ◽  
DanielJ. Clauw ◽  
RonaldB. Natale ◽  
RaymondW. Ruddon
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytotoxic Effects

Exosomes derived from human bone marrow mesenchymal stem cells promote tumor growth in vivo

2012 ◽  
Vol 315 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Wei Zhu ◽  
Ling Huang ◽  
Yahong Li ◽  
Xu Zhang ◽  
Jianmei Gu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Tumor Growth ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Promote Tumor Growth ◽  
Marrow Mesenchymal Stem Cells

In Vivo Effects of Human Bone Marrow Mesenchymal Stromal Cells on the Development of Experimental B16 Melanoma in Mice

2020 ◽  
Vol 168 (4) ◽  
pp. 561-565 ◽  
Author(s):  
V. N. Petrov ◽  
E. V. Isaeva ◽  
S. E. Ulyanenko ◽  
E. E. Beketov ◽  
E. M. Yatsenko ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
B16 Melanoma ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vivo Effects

Effectiveness of Cryo-Preserved Human Bone Marrow Stromal Cells at Forming Bone in Vivo

2005 ◽  
Vol 116 (Supplement) ◽  
pp. 150-151
Author(s):  
Mahesh H. Mankani ◽  
Sergei Kuznetsov ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

The in vivo profile of transcription factors during neutrophil differentiation in human bone marrow

Blood ◽  
2003 ◽  
Vol 101 (11) ◽  
pp. 4322-4332 ◽  
Author(s):  
Malene Digmann Bjerregaard ◽  
Jesper Jurlander ◽  
Pia Klausen ◽  
Niels Borregaard ◽  
Jack Bernard Cowland
Keyword(s):  
Bone Marrow ◽  
Transcription Factors ◽  
Protein Extraction ◽  
Magnetic Bead ◽  
Human Bone ◽  
Polymorphonuclear Neutrophils ◽  
Human Bone Marrow ◽  
Ccaat Displacement Protein ◽  
Mature Neutrophil

Abstract In vivo distribution of myeloid transcription factors during granulopoiesis was investigated by Northern and Western blotting in 3 neutrophil precursor populations from human bone marrow: immature (myeloblasts [MBs] and promyelocytes [PMs]); intermediate mature (myelocytes [MCs] and metamyelocytes [MMs]); and mature neutrophil cells (band cells [BCs] and segmented neutrophil cells [SCs]). Nonneutrophil cells were removed with magnetic-bead–coupled antibodies against CD2, CD3, CD14, CD19, CD56, CD61, glycophorin-A, and CD49d (BCs/SCs) before RNA and protein extraction. Polymorphonuclear neutrophils (PMNs) from peripheral blood depleted with anti-CD49d antibodies were also included. Expression of acute myeloid leukemia 1b (AML-1b), c-myb, GATA-1, and CCAAT/enhancer binding protein γ (C/EBP-γ) was seen primarily in MBs/PMs, and little expression was found in more mature cells. The level of C/EBP-α was constant in the bone marrow–derived cells and decreased in PMNs. C/EBP-ϵ was found primarily in MCs/MMs and was almost absent in more mature cells. Expression of C/EBP-β, C/EBP-δ, and C/EBP-ζ was observed from the MC/MM stage onward, with peak levels in the most mature cells. The amount of PU.1 increased throughout maturation whereas the level of Elf-1 reached a nadir in MCs/MMs The PU.1 coactivator c-jun and c-jun's dimerization partner c-fos were both detectable in MCs/MMs and increased in amount with maturity. CCAAT displacement protein (CDP) was found at comparable levels at all stages of differentiation. This demonstrates a highly individualized expression of the transcription factors, which can form the basis for the heterogeneous expression of granule proteins during granulopoiesis and cell cycle arrest in metamyelocytes.

Human Bone Marrow MSCs form Cartilage and Mineralized Tissue on Chitosan/Polycaprolactone (CS/PCL) Combined Nanofibrous Scaffolds

2017 ◽  
Vol 17 (3) ◽  
pp. 1771-1778 ◽  
Author(s):  
Sarah Abuelreich ◽  
Muthurangan Manikandan ◽  
Abdullah Aldahmash ◽  
Musaad Alfayez ◽  
Mohammed Fayez Al Rez ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Bone Marrow Mscs ◽  
Mineralized Tissue ◽  
Nanofibrous Scaffolds ◽  
Human Bone Marrow Mscs

scholarly journals Implantation and maintenance of functional human bone marrow in SCID-hu mice

Blood ◽  
1992 ◽  
Vol 79 (7) ◽  
pp. 1704-1711 ◽  
Author(s):  
S Kyoizumi ◽  
CM Baum ◽  
H Kaneshima ◽  
JM McCune ◽  
EJ Yee ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Activity ◽  
Direct Analysis ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Multilineage Differentiation ◽  
Bone Implants ◽  
Human Erythropoietin ◽  
Bone Fragments

Abstract Human fetal bone fragments implanted in the immunodeficient C.B-17 scid/scid (SCID) mouse were shown to sustain active human hematopoiesis in vivo. Human progenitor cell activity was maintained for as long as 20 weeks after implantation and was associated with multilineage differentiation in the engrafted bone. Thus, the bone implants provided stem cells as well as the microenvironment requisite for their long- term maintenance and multilineage differentiation. Administration of human erythropoietin (Epo) stimulated human erythropoiesis in human bone implants. This animal model may facilitate direct analysis of a wide variety of physiologic and pathologic conditions of human bone marrow (BM) in vivo.

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