scholarly journals A Laboratory Assessment of Two Local Strains of the Beauveria bassiana (Bals.) Vuill. against the Tetranychus urticae (Acari: Tetranychidae) and Their Potential as a Mycopesticide

2017 ◽  
Vol 2017 ◽  
pp. 1-7
Author(s):  
Serkan Ortucu ◽  
Omer Faruk Algur
Keyword(s):  
Beauveria Bassiana ◽  
Uv Radiation ◽  
Mass Production ◽  
Spore Production ◽  
Laboratory Assessment ◽  
Highly Pathogenic ◽  
Different Temperatures ◽  
Rdna Analysis ◽  
Production Tolerance ◽  
Disk Area

This study was conducted to assess highly pathogenic Beauveria bassiana isolates to be used in biocontrol and to determine their potentials as mycopesticide. For this purpose, two B. bassiana isolates, which were locally isolated from T. urticae, were chosen. Firstly, three suspensions were investigated at the degree of humidity of 65 ± 5% and 100% RH. Secondly, these strains were selected according to their tendency to mass production, tolerance to UV radiation, and capability of producing spore at the different temperatures. Finally, identification of the selected isolate was performed by using ITS rDNA analysis. Both tested fungal isolates were pathogenic to the T. urticae. Mycelial growths of isolate AT076 at 20°C and 30°C were found to be greater than isolate AT007. It was observed that isolate AT076 had more spore production with 1.61 × 107 spore/disc at 30°C and 44.33% germination after UV radiation for 15 min. The numbers of spores per 5 mm disk area for isolates AT076 and AT007 were found to be 1.2 × 106 and 1.0 × 106. These results show that isolate AT076 was more virulent and more UV-tolerant and had higher tendency to mass production compared to isolate AT007 against T. urticae. As a result of this study, isolate AT076 can be used in the biocontrol as mycopesticide.

scholarly journals The temperature of growth and sporulation modulates the efficiency of spore-display in Bacillus subtilis

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Claudia Petrillo ◽  
Stefany Castaldi ◽  
Mariamichela Lanzilli ◽  
Anella Saggese ◽  
Giuliana Donadio ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Spore Production ◽  
Bacterial Spores ◽  
Heterologous Proteins ◽  
Dna Coding ◽  
Gene Coding ◽  
Different Temperatures ◽  
Mucosal Vaccines ◽  
Heterologous Antigens ◽  
Selection Of

Abstract Background Bacterial spores displaying heterologous antigens or enzymes have long been proposed as mucosal vaccines, functionalized probiotics or biocatalysts. Two main strategies have been developed to display heterologous molecules on the surface of Bacillus subtilis spores: (i) a recombinant approach, based on the construction of a gene fusion between a gene coding for a coat protein (carrier) and DNA coding for the protein to be displayed, and (ii) a non-recombinant approach, based on the spontaneous and stable adsorption of heterologous molecules on the spore surface. Both systems have advantages and drawbacks and the selection of one or the other depends on the protein to be displayed and on the final use of the activated spore. It has been recently shown that B. subtilis builds structurally and functionally different spores when grown at different temperatures; based on this finding B. subtilis spores prepared at 25, 37 or 42 °C were compared for their efficiency in displaying various model proteins by either the recombinant or the non-recombinant approach. Results Immune- and fluorescence-based assays were used to analyze the display of several model proteins on spores prepared at 25, 37 or 42 °C. Recombinant spores displayed different amounts of the same fusion protein in response to the temperature of spore production. In spores simultaneously displaying two fusion proteins, each of them was differentially displayed at the various temperatures. The display by the non-recombinant approach was only modestly affected by the temperature of spore production, with spores prepared at 37 or 42 °C slightly more efficient than 25 °C spores in adsorbing at least some of the model proteins tested. Conclusion Our results indicate that the temperature of spore production allows control of the display of heterologous proteins on spores and, therefore, that the spore-display strategy can be optimized for the specific final use of the activated spores by selecting the display approach, the carrier protein and the temperature of spore production.

Solar Energy Collection With Evacuated Tubes

1965 ◽  
Vol 87 (3) ◽  
pp. 270-276 ◽  
Author(s):  
E. Speyer
Keyword(s):  
Solar Energy ◽  
Mass Production ◽  
Air Conditioning ◽  
Tube Wall ◽  
Glass Tube ◽  
Different Temperatures ◽  
Evacuated Tubes ◽  
Liquid Flows ◽  
Evacuated Tube ◽  
Economic Considerations

Engineering and economic considerations are given for nontracking solar energy collectors where the absorbing surface is insulated by an evacuated space. Each collector is a long glass tube, about two in. in dia, silvered in the lower portion. The selectively blackened conduit through which liquid flows is suspended within the evacuated tube, passing through the tube wall at only one end. Prototype tubular collectors were built, and tested when evacuated and when air-filled. The measured efficiencies at different temperatures are given, and the data fitted by empirical equations. Costs per tube and per Btu are given together with an indication of applicability to solar air conditioning. Mass production of these collectors will depend on the indicated demand.

Compatibility of Zineb, Dimethoate and Beauveria bassiana (Balsamo) Vuillemin Against Tarnished Plant Bug (Hemiptera: Miridae)

2003 ◽  
Vol 38 (3) ◽  
pp. 359-367 ◽  
Author(s):  
Mathias Kouassi ◽  
Daniel Coderre ◽  
Silvia I. Todorova
Keyword(s):  
Beauveria Bassiana ◽  
Adult Mortality ◽  
Lygus Lineolaris ◽  
Phenological Stage ◽  
Highly Pathogenic ◽  
Tarnished Plant Bug ◽  
Lactuca Sativa L ◽  
Dimethyl Dithiocarbamate ◽  
Laboratory Bioassays

Experiments were conducted under laboratory and field conditions to assess the effect of the fungicide Zineb (zinc dimethyl-dithiocarbamate) and the insecticide Cygon (dimethoate) on the pathogenicity of the isolate MK 2001 of Beauveria bassiana (Balsamo) Vuillemin, a potential microbial agent for biological control of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois). The isolate was highly pathogenic to adults yielding an LC50 of 7.2 × 103 conidia/mL at 6 d post treatment and an LT50 of 3.07 d at a concentration of 1 × 108 conidia/mL. Zineb inhibited the in vitro growth of the fungus in Sabouraud's dextrose broth and on Sabouraud's dextrose agar. It had no insecticidal activity against adult L. lineolaris in laboratory bioassays. A field test with caged L. lineolaris adults on lettuce Lactuca sativa L. var. longifolia, at a phenological stage of 50 leaves further demonstrated that Zineb and Dimethoate had no effect on B. bassiana against L. lineolaris. Adult mortality 10 d after application did not differ significantly in treatments with B. bassiana alone from treatments with B. bassiana + Zineb, dimethoate alone, B. bassiana + dimethoate, B. bassiana + Zineb, and B. bassiana + Zineb + dimethoate. No larva was observed in cages treated with B. bassiana alone or in combination with Zineb or dimethoate. On the contrary, 11 larvae/plant were recorded in the untreated cages and 5 larvae/plant in the dimethoate-treated cages.

Evaluate on substrates for mass production of entomopathogenic fungus Beauveria bassiana (Balsamo)

2019 ◽  
Vol 4 (01) ◽  
pp. 84-88
Author(s):  
Rajendra Singh ◽  
Vijay Pratap Singh ◽  
Ajay Kumar ◽  
Joginder Singh ◽  
Ankit Kumar ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Beauveria Bassiana ◽  
Vigna Unguiculata ◽  
Mass Production ◽  
Block Design ◽  
Vigna Mungo ◽  
Black Gram ◽  
Potato Dextrose Broth ◽  
Solid Media ◽  
Randomized Block Design

The present investigation entitled “Mass Production of entomopathogenic fungai Beauveria bassiana (Metsch.) on liquid and solid media” was carried out at Bio Control Laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut during 2018-19. In all there were eight treatments including control replicated thrice in a randomized block design. The results obtained in the study are summarized. The effect of different substrates for the mass production of Beauveria bassiana spore/ml was significantly higher recorded. Data recorded on 7, 14, 21, 28, 35 after the day after inoculation of various treatments in the, T10 Savoured dextrose broth (SDB), was the best treatment by bringing down the B. Bassiana production up to (49.48) spore/ml in liquid medium during the 2018-19 years. The other treatments in order of spore producing was with T11 Potato dextrose broth (PDB) (38.52), in liquid medium and solid medium in the treatment and T8 Cowpea Vigna unguiculata + Molasses + 1% YE + 1.0 g Dextrose (28.73), followed by with T7 Black gram Vigna mungo + Molasses + 1% YE + 1.0 g Dextrose (27.52 spore/ml, T5 Cowpea Vigna unguiculata + Molasses + 1% YE (17.53 spore/ml), T4 Black gram Vigna mungo + Molasses + 1% YE (14.87 spore/ml), T2 Cowpea Vigna unguiculata + Molasses (10.50 spore/ml), T1 Black gram Vigna mungo + Molasses (8.35 spore/ml), T9 Gram Cicer aeritinum + Molasses + 1% YE + 1.0 g Dextrose (3.92 spore/ml), T6 Gram Cicer aeritinum + Molasses + 1% YE (2.85 spore/ml) and T3 Gram Cicer aeritinum + Molasses respectively.

Sign in / Sign up

Export Citation Format

Share Document