scholarly journals Synthesis of Poly-(R-hydroxyalkanoates) byCupriavidus necatorATCC 17699 Using Mexican Avocado(Persea americana)Oil as a Carbon Source

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Araceli Flores-Sánchez ◽  
Ma. del Rocío López-Cuellar ◽  
Fermín Pérez-Guevara ◽  
Ulises Figueroa López ◽  
José Mauricio Martín-Bufájer ◽  
...  
Keyword(s):  
Carbon Source ◽  
Bacterial Species ◽  
Persea Americana ◽  
Growth Stages ◽  
Vast Number ◽  
Chromatography Analysis ◽  
Avocado Oil ◽  
Gas Chromatography Analysis ◽  
Third Stage ◽  
Pha Production

Poly-R-hydroxyalkanoates (PHAs) are polymers produced by a vast number of bacterial species under stress conditions. PHAs exhibit different thermal and mechanical properties that depend on their molecular structure. In this work, PHAs were produced using avocado oil as the carbon source.Cupriavidus necatorH16 was cultured in three-stage fermentation using fructose during the cell growth stages and avocado oil during the PHA synthesis stage. Different concentrations of avocado oil were used during the third stage to test the incorporation of various monomeric units into the PHAs. Biomass and PHA production were measured during the fermentation. DSC, FTIR, and gas chromatography analysis aided the PHA characterization. Different proportions of 3-hydroxyvalerate were present in the 3-hydroxybutyrate main chain depending on the concentration of avocado oil. The results suggest that avocado oil is a viable new substrate for PHA production.

scholarly journals Is There Daily Growth Hysteresis versus Vapor Pressure Deficit in Cherry Fruit?

Horticulturae ◽  
2021 ◽  
Vol 7 (6) ◽  
pp. 131
Author(s):  
Matteo Zucchini ◽  
Arash Khosravi ◽  
Veronica Giorgi ◽  
Adriano Mancini ◽  
Davide Neri
Keyword(s):  
Vapor Pressure ◽  
Vapor Pressure Deficit ◽  
Fruit Growth ◽  
Hysteresis Curve ◽  
Growth Stages ◽  
Fourth Stage ◽  
The Third ◽  
Third Stage ◽  
Rainy Days ◽  
Clockwise Hysteresis

The growth of cherry fruit is generally described using a double sigmoid model, divided into four growth stages. Abiotic factors are considered to be significant components in modifying fruit growth, and among these, the vapor pressure deficit (VPD) is deemed the most effective. In this study, we investigated sweet cherry fruit growth through the continuous, hourly monitoring of fruit transversal diameter over two consecutive years (2019 and 2020), from the beginning of the third stage to maturation (forth stage). Extensometers were used in the field and VPD was calculated from weather data. The fruit growth pattern up to the end of the third stage demonstrated three critical steps during non-rainy days: shrinkage, stabilization and expansion. In the third stage of fruit growth, a partial clockwise hysteresis curve of circadian growth, as a response to VPD, appeared on random days. The pattern of fruit growth during rainy days was not distinctive, but the amount and duration of rain caused a consequent decrease in the VPD and indirectly boosted fruit growth. At the beginning of the fourth stage, the circadian growth changed and the daily transversal diameter vs VPD formed fully clockwise hysteresis curves for most of this stage. Our findings indicate that hysteresis can be employed to evaluate the initial phenological phase of fruit maturation, as a fully clockwise hysteresis curve was observable only in the fourth stage of fruit growth. There are additional opportunities for its use in the management of fruit production, such as in precision fruit farming.

Avocado oil ( Persea americana ) protects SH‐SY5Y cells against cytotoxicity triggered by cortisol by the modulation of BDNF, oxidative stress, and apoptosis molecules

2021 ◽  
Vol 45 (2) ◽  
Author(s):  
Jéssica Rosso Motta ◽  
Ivo Emilio da Cruz Jung ◽  
Verônica Farina Azzolin ◽  
Cibele Ferreira Teixeira ◽  
Luiza Elizabete Braun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Persea Americana ◽  
Avocado Oil

Use of bacteriostatic antibiotics for the optimization of new media in view of supplementing POME as an alternative carbon source for PHA production - a statistical approach

2019 ◽  
Vol 16 ◽  
pp. 1692-1701
Author(s):  
Ponnaiah Paulraj ◽  
Harvie Anak Shukri ◽  
Vnootheni Nagiah ◽  
Nagaraja Suryadevara ◽  
Balavinayagamani Ganapathy
Keyword(s):  
Carbon Source ◽  
New Media ◽  
Statistical Approach ◽  
Alternative Carbon Source ◽  
Pha Production

Diversity of Root Bacteria from Peanut Cropping Systems

2004 ◽  
Vol 31 (2) ◽  
pp. 86-91 ◽  
Author(s):  
D. T. Gooden ◽  
H. D. Skipper ◽  
J. H. Kim ◽  
K. Xiong
Keyword(s):  
Fatty Acids ◽  
Biological Control ◽  
Gas Chromatography ◽  
Cropping Systems ◽  
Cropping System ◽  
Critical Research ◽  
Chromatography Analysis ◽  
Rotation System ◽  
Gas Chromatography Analysis ◽  
Peanut Production

Abstract Rhizobacteria play an important role in sustainable agriculture via plant growth and biological control of pests in a number of ecosystems. Understanding the interactions of crop rotation and rhizobacteria on peanut production is a critical research need. Development of a database on the rhizobacteria obtained from continuous and rotational fields of peanut was initiated in 1997 and terminated in 2000. Peanut was planted in monoculture for 4 yr. In rotational plots, peanut, cotton, corn, and peanut were planted in sequence. Rhizobacteria were isolated from the roots of crop plants grown in a Norfolk soil near Florence, SC. These isolates were identified by composition of fatty acids from gas chromatography analysis (GC/FAME). Arthrobacter and Bacillus were the major genera from non-rhizosphere soils. At initiation of this study in July 1997, the plots selected for continuous peanut had more diversity in rhizobacteria than those plots selected for rotation. In July 2000, rhizobacteria diversity was greater from peanut roots in the rotation cropping system than continuous peanut. Even though rhizobacteria diversity was greater in the rotation system, higher peanut yields were recorded in the continuous peanut system in 2000. Burkholderia spp. were always isolated from the peanut and other crop rhizospheres at each sampling date.

scholarly journals Musa balbisiana and Musa paradisiaca Starches Increase SCFA and Caspase-3 as well as Decrease β-glucuronidase and MDA of Mouse Model for Colon Cancer

2021 ◽  
Vol 13 (1) ◽  
pp. 91-6
Author(s):  
Diana Nur Afifah ◽  
Fauzia Purnamasari ◽  
Luthfiatul Khusna ◽  
Noviasti Rahma Utami ◽  
Aida Fitri Nazillah ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Mouse Model ◽  
Resistant Starch ◽  
Caspase 3 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Musa Balbisiana ◽  
Chromatography Analysis ◽  
Musa Paradisiaca ◽  
Gas Chromatography Analysis ◽  
Low Glycemic Index

BACKGROUND: Administration of resistant starch (RS) influences the diversity and the composition of microbiota as well as inhibits the growth of cancer cell. Banana as a potential source of RS has been reported. Although Musa paradisiaca has been reported to induce apoptosis in colon cancer cells, Musa balbisiana, which has low glycemic index and suitable for particular patients, has not been investigated yet.METHODS: Starches of M. balbisiana and M. paradisiaca were prepared and mixed with other components to make 3 types of mouse pellets. Mouse model for colon cancer was prepared and fed with different types of mouse pellets. Blood was collected and processed for measuring β-glucuronidase and malondialdehyde (MDA) with Enzyme-linked Immunosorbent Assay (ELISA) method. Resected ceca were incised to collect the inner part for short-chain fatty acid (SCFA) measurement with gas chromatography analysis. Resected colas were fixed and processed for immunohistochemistry to detect Caspase-3.RESULTS: Colon-cancer-mice fed with the M. balbisiana and M. paradisiaca starches-contained pellets had significant higher concentrations of total SCFA (p=0.003), acetic acid (p=0.000), propionic acid (p=0.000) and butyric acid (p=0.000); lower concentration of β-glucuronidase (p<0.001); higher Caspase-3 score (p=0.040); and lower MDA concentration (p<0.001) than colon-cancer-mice fed with standard pellet (control).CONCLUSION: M. balbisiana and M. paradisiaca starches could be suggested as potential anti-colon cancer RS. Further research should be carried out to disclose the starches mechanisms in colon cancer cell.KEYWORDS: Musa balbisiana, Musa paradisiaca, colon cancer, resistant starch, Caspase-3, SCFA, β-glucuronidase, malondialdehyde

scholarly journals Anti-arthritic and gastroprotective activities of Ardisia crispa root partially mediated via its antioxidant effect

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Roslida Abdul Hamid ◽  
Lau Moi Fong ◽  
Yeong Looi Ting
Keyword(s):  
Antioxidant Activities ◽  
Inflammatory Diseases ◽  
Dose Range ◽  
Antioxidant Effect ◽  
Total Phenolic ◽  
Chromatography Analysis ◽  
Gas Chromatography Analysis ◽  
Tnf Α ◽  
Ulcer Model ◽  
Lower Dose Range

AbstractBackgroundThunb A.DC (Myrsinaceae), commonly known as “hen’s eyes”, has been traditionally used in treating various inflammatory diseases. The present study evaluated anti-arthritic, gastroprotective and antioxidant activities ofMethodsAnti-arthritic activity was evaluated in complete Freund adjuvant (CFA)-induced adjuvant arthritis and gastroprotective effect was studied in the ethanol-induced ulcer model in rats. ACRH was further isolated to yield quinone-rich fraction (QRF) and both were analyzed for their total phenolic content, total flavonoid content and antioxidant activities in various antioxidant assays. Both ACRH and QRF were also analyzed for the quinone composition via gas chromatography analysis.ResultsACRH exerted significant reduction of IL-1β and TNF-α at a lower dose range in CFA-induced arthritis, as well as exhibited its cytoprotective effect against ethanol-induced ulcer lesion via involvement of mucosal nonprotein sulfhydryl (NP-SH) groups. ACRH also showed higher phenolic and flavonoid contents, as well as better antioxidant activities than QRF.ConclusionsThese findings demonstrated the plant as a potential anti-inflammatory agent, with ACRH succeeded in inhibiting both arthritic and ulcerogenic effect, possibly mediated via its antioxidant effect.

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