scholarly journals Basidiobolomycosis Simulating a Mycobacterium ulcerans Infection in a Togolese Rural Child

2017 ◽  
Vol 2017 ◽  
pp. 1-3 ◽  
Author(s):  
Bayaki Saka ◽  
Waguena Gnassingbe ◽  
Garba Mahamadou ◽  
Sefako Akakpo ◽  
Julienne Teclessou ◽  
...  
Keyword(s):  
General Condition ◽  
Buruli Ulcer ◽  
Granulomatous Inflammation ◽  
Mycobacterium Ulcerans ◽  
Chain Reaction ◽  
Deep Mycosis ◽  
Sharp Edges ◽  
Polymerase Chain ◽  
The Right ◽  
Multiple Ulcers

Background. Basidiobolomycosis is a deep mycosis which preferentially affects rural young people in tropical countries. We report an atypical case, with multiple ulcers, simulating a Buruli ulcer. Case Report. A 5-year-old boy, living in a rural area, was seen for ulcers on the buttocks and at the back and right flank that had been in progress for 4 months. On examination, we found an infiltrated plaque with sharp edges, little painful, located on the buttocks, back, and the right flank. On this plaque, there were multiple ulcers with polycyclic contours and fibrinous bottom. There were inguinal inflammatory lymph nodes. The patient had an altered general condition. Examination of other organs was normal. The diagnosis of Buruli ulcer was evoked first; the search for Mycobacterium ulcerans by polymerase chain reaction was negative. Histology test performed revealed hypodermic granulomatous inflammation with predominant macrophage and eosinophils. The mycological culture was not done. The child was treated successfully with ketoconazole (10 mg/kg/day) during eight weeks. Discussion. Our observation shows great clinical and epidemiological similarities between basidiobolomycosis and Buruli ulcer. It confirms the efficacy of ketoconazole in severe basidiobolomycosis infection with alteration of general condition. Histopathology is very important for differential diagnosis between these two diseases.

Histopathological and polymerase chain reaction-based analyses of Buruli ulcer caused by Mycobacterium ulcerans subspecies shinshuense

2017 ◽  
Vol 86 (2) ◽  
pp. e39
Author(s):  
Toshifumi Takahashi ◽  
Noriki Fujimoto ◽  
Miho Kabuto ◽  
Takeshi Kato ◽  
Takeshi Nakanishi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Buruli Ulcer ◽  
Mycobacterium Ulcerans ◽  
Chain Reaction ◽  
Polymerase Chain

Confirming Autochthonous Buruli Ulcer Cases in Burkina Faso, West Africa

2021 ◽  
Author(s):  
Anselme Millogo ◽  
Dezemon Zingue ◽  
Amar Bouam ◽  
Sylvain Godreuil ◽  
Michel Drancourt ◽  
...  
Keyword(s):  
West Africa ◽  
Burkina Faso ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Buruli Ulcer ◽  
Mycobacterium Ulcerans ◽  
Chain Reaction ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Polymerase Chain

Environmental Mycobacterium ulcerans causes a disabling skin disease called Buruli ulcer. Recent studies completed the knowledge of the evolving geographic extension and epidemiology of Buruli ulcer in West Africa, where Côte d’Ivoire is reporting the highest number of cases. We report seven polymerase chain reaction-documented patients in Burkina Faso, a neighboring country of Côte d’Ivoire, where previously Buruli ulcer cases were confirmed primarily using clinical arguments.

scholarly journals Malacoplakia of the Uterine Cervix: A Case Report

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 343
Author(s):  
Adela Saco ◽  
Natalia Rakislova ◽  
Lorena Marimon ◽  
Aureli Torne ◽  
Berta Diaz-Feijoo ◽  
...  
Keyword(s):  
Uterine Cervix ◽  
Genital Tract ◽  
Vaginal Bleeding ◽  
Female Genital Tract ◽  
Granulomatous Inflammation ◽  
Resonance Imaging ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Cervical Tumors ◽  
Female Genital

Malacoplakia is an uncommon chronic granulomatous inflammation that rarely affects the female genital tract. A case of a 78-year-old woman with malacoplakia involving the uterine cervix and the vagina is described. The patient complained of vaginal bleeding. Clinically, a 13-mm mass was detected in the cervix, which was confirmed by ultrasound scan and magnetic resonance imaging. Histological examination showed a dense histiocytic infiltrate with abundant Michaelis–Gutmann bodies involving the uterine cervix and the upper vagina. The presence of Escherichia coli was confirmed in the lesion by immunohistochemistry and polymerase chain reaction. Only 12 cases of cervical malacoplakia have been reported to date. This condition should be included in the differential diagnosis of cervical tumors.

scholarly journals Refractory pemphigus vulgaris associated with herpes infection: case report and review

2011 ◽  
Vol 53 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Maria Luiza Figueiredo Braga Brandão ◽  
Nurimar C. Fernandes ◽  
Danielle Pereira De Oliveira Batista ◽  
Norma Santos
Keyword(s):  
Sequence Analysis ◽  
Pemphigus Vulgaris ◽  
Chain Reaction ◽  
Upper Eyelid ◽  
Triggering Factor ◽  
Polymerase Chain ◽  
Genetic And Environmental Factors ◽  
The Right ◽  
Hsv 1

BACKGROUND: Pemphigus vulgaris (PV) is an autoimmune disease characterized by blistering of the skin and mucosa, which develops due to the interaction between predisposing genetic and environmental factors. Infections caused by members of the Herpesviridae family have been suggested as a possible triggering factor for PV. OBJECTIVE AND METHODS: In this report, we investigate the presence of herpesviruses in refractory lesions on the right upper eyelid. The lesion has persisted despite the treatment with corticosteroids. Polymerase chain reaction (PCR) and DNA sequence analysis have been used to detect the DNA of HSV 1/2, VZV, EBV, CMV, HHV-6, HHV-7, and HHV-8. RESULTS: The sample collected from the right upper eyelid has tested positive for HSV 1/2. Sequence analysis has confirmed the PCR results and allowed the identification of the HSV strain as belonging to type 1. After treatment with acyclovir, the lesion of the right upper eyelid has cleared and not relapsed. CONCLUSION: When patients present PV lesions which are refractory to corticosteroid therapy, herpetic infection should be considered.

scholarly journals Adult gonococcal keratoconjunctivitis: early detection is key

2020 ◽  
Vol 17 (1) ◽  
pp. 30-34
Author(s):  
Daniel Lai ◽  
Keith Ong
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Neisseria Gonorrhoeae ◽  
Ophthalmic Solution ◽  
Careful Monitoring ◽  
Corneal Ulceration ◽  
Chain Reaction ◽  
Bacterial Conjunctivitis ◽  
Polymerase Chain ◽  
The Right

We describe a case of a 52-year-old male presenting with severe mucopurulent conjunctivitis of the right eye. Corneal ulceration and associated anterior chamber activity was noted later in the course of the disease. Neisseria gonorrhoeae was positive on polymerase chain reaction (PCR) testing earlier than traditional microscopy and culture. He was successfully treated with ceftriaxone 500 mg intravenously and azithromycin 1 g orally as single doses in addition to ofloxacin ophthalmic solution 0.3% hourly to the right eye. This case highlights the need to consider the possibility of gonococcus in cases of suspected bacterial conjunctivitis, careful monitoring for corneal involvement and the importance of early detection with PCR.

scholarly journals Vibrio cholerae non-O1 - the first reported case of keratitis in a healthy patient

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wei-Dar Chen ◽  
Li-Ju Lai ◽  
Wei-Hsiu Hsu ◽  
Tsung-Yu Huang
Keyword(s):  
Vibrio Cholerae ◽  
Corneal Ulcer ◽  
Positive Outcome ◽  
Ocular Injury ◽  
Significant Morbidity ◽  
Chain Reaction ◽  
Case Presentation ◽  
First Case ◽  
Polymerase Chain ◽  
The Right

Abstract Background Vibrio cholerae non-O1 is a virulent pathogen that causes significant morbidity and mortality in humans. Herein, we report a case of corneal ulcer caused by this pathogen. Case presentation A 59-year-old fisherman with no systemic history was struck in the right eye by a marine shrimp and developed keratitis. Corneal scrapping culture revealed the presence of the V. cholerae non-O1, and its identification was confirmed by Analytical Profile Index 20E system and polymerase chain reaction. He was successfully treated with topical levofloxacin (0.3%) and fortified amikacin (12.5 mg/mL) for 2 weeks. The visual acuity recovered to 20/25 after treatment without complications. Conclusions This is the first case report of keratitis caused by V. cholerae non-O1 strain. Ocular injury by marine creatures and contaminated seawater can contribute to severe corneal ulcer. Early diagnosis can be achieved by meticulous history taking and a comprehensive laboratory workup. Simultaneously, an effective antibiotic therapy can lead to a positive outcome.

Buruli ulcer: Mycobacterium ulcerans infection

2020 ◽  
pp. 1167-1170
Author(s):  
Bouke de Jong ◽  
Françoise Portaels ◽  
Wayne M. Meyers
Keyword(s):  
Polymerase Chain Reaction ◽  
Subcutaneous Tissue ◽  
Buruli Ulcer ◽  
Central Africa ◽  
Skin Grafting ◽  
Surgical Excision ◽  
Mycobacterium Ulcerans ◽  
West And Central Africa ◽  
Cutaneous Nodule ◽  
Polymerase Chain

Buruli ulcer is caused by Mycobacterium ulcerans, which secretes a cytotoxic and immunosuppressive toxin, mycolactone. The disease is characterized by necrosis of skin, subcutaneous tissue, and bone, and is re-emerging as a potentially disabling affliction of inhabitants of tropical wetlands. Major foci are in West and Central Africa with an increasing focus in Australia, Mexico, South America, and Southeast Asia. It is not contagious; environmental sources include water, vegetation, and insects, with humans probably becoming infected by traumatic introduction of the bacillus into the skin from the overlying M. ulcerans-contaminated surface in most instances. Clinical presentation may be as a cutaneous nodule, undermined ulcer, plaque, or widely disseminated oedematous lesion. Clinical diagnosis is often accurate by experienced clinicians, and smears for acid-fast bacilli, culture, polymerase chain reaction assays, and histopathology are confirmatory. Treatment was formerly by wide surgical excision and skin grafting, yet antibiotics have now been found effective, including an all-oral regimen.

Expression of WT1 in Pediatric Small Cell Tumors: Report of Two Cases with a Possible Mesothelial Origin

1999 ◽  
Vol 2 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Paul Thorner ◽  
Jeremy Squire ◽  
Natasha Plavsic ◽  
Roland Jong ◽  
Mark Greenberg ◽  
...  
Keyword(s):  
Wilms Tumor ◽  
Small Cell ◽  
Rhabdoid Tumor ◽  
Round Cell ◽  
Rt Pcr ◽  
Fetal Kidney ◽  
Chain Reaction ◽  
Mesenchymal Phenotype ◽  
Polymerase Chain ◽  
The Right

The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. We examined WT1 expression levels by reverse-transcriptase polymerase chain reaction (RT-PCR) in 38 childhood small-cell tumors including Wilms tumor, embryonal and alveolar rhabdomyosarcoma, Ewing sarcoma, lymphoma, desmoplastic small round-cell tumor (DSRCT), synovial sarcoma, extrarenal rhabdoid tumor, and two tumors that were atypical for this group of tumors. WT1 expression was only detected in Wilms tumor, rhabdoid tumor, and in these two cases of uncertain histogenesis. Both arose in the peritoneal cavity and by immunohistochemistry were diffusely positive for vimentin, keratin, and desmin. Tonofilaments were identified by electron microscopy in one of the cases. RT-PCR failed to detect the t(11;22) translocation associated with DSRCT in either case. Our results suggest that WT1 expression is an unusual feature of childhood non-Wilms tumors and, in the right setting, it may indicate a mesothelial origin. The expression of WT1 may play a role in mesodermal cells acquiring epithelial characteristics, a concept supported by the mixed epithelial and mesenchymal phenotype of these two cases.

Tuberculosis of the glans penis: an important differential diagnosis of genital ulcer disease

2017 ◽  
Vol 28 (14) ◽  
pp. 1453-1455 ◽  
Author(s):  
Archana Singal ◽  
Deepika Pandhi ◽  
Vandana Kataria ◽  
Vinod K Arora
Keyword(s):  
Differential Diagnosis ◽  
Glans Penis ◽  
Chain Reaction ◽  
Genital Tuberculosis ◽  
Ulcer Disease ◽  
Important Differential Diagnosis ◽  
Polymerase Chain ◽  
Systemic Antibiotics ◽  
Multiple Ulcers ◽  
Apparently Healthy

We report a 45-year-old, apparently healthy sero-negative man, presenting with multiple ulcers on the glans penis for a duration of three months. There was no significant inguinal lymphadenopathy. He showed no improvement on systemic antibiotics and acyclovir. Histopathology revealed the diagnosis of genital tuberculosis (TB), and polymerase chain reaction for Mycobacterium tuberculosis tested positive. The patient responded well to category I anti-tubercular treatment with complete resolution of lesions in six months. It is important to consider a differential diagnosis of penile TB in patients with non-healing genital ulcers.

Sign in / Sign up

Export Citation Format

Share Document