scholarly journals The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Menglei Wang ◽  
Meixuan Li ◽  
Pisong Yan ◽  
Qiang Luo ◽  
Yu Zhang ◽  
...  
Keyword(s):  
High Intensity ◽  
Focused Ultrasound ◽  
Collagen Type ◽  
Anterior Segment ◽  
Collagen Type I ◽  
High Intensity Focused Ultrasound ◽  
Type I ◽  
Corneal Cells ◽  
The Right ◽  
Corneal Collagen

Purpose.To evaluate the safety of high-intensity focused ultrasound keratoplasty as a treatment for presbyopia by examining its effect on the rabbit anterior segment.Methods.The right corneas of 36 New Zealand rabbits were treated with HIFU keratoplasty. The animals were sacrificed at 1, 7, 15, 30, 60, and 90 days after operation. Collagen type I, MMP-2, and MMP-9 were evaluated using immunohistochemistry. For the detection of apoptosis, the TUNEL method was applied. The SOD and MDA levels were analyzed with assay kits.Results.Collagen type I, MMP-2, and MMP-9 levels were altered after the operation but returned to normal within 90 days. The apoptotic index (AI) of the corneal cells decreased from 1 to 30 days gradually. No apoptosis was observed in the epithelial cells of the lens, and the SOD and MDA levels were normal at any time point.Conclusion.After HIFU keratoplasty, the histomorphology of the cornea changed, the corneal collagen type I levels decreased, the corneal MMP-2 and MMP-9 levels increased, and the corneal cells underwent apoptosis for a period of time. Ninety days after the operation, the levels returned to normal, and the lenses were not affected. Thus, HIFU presents good biological safety for eyes.

Targeting accuracy of transcranial magnetic resonance–guided high-intensity focused ultrasound brain therapy: a fresh cadaver model

2013 ◽  
Vol 118 (5) ◽  
pp. 1046-1052 ◽  
Author(s):  
Dorian Chauvet ◽  
Laurent Marsac ◽  
Mathieu Pernot ◽  
Anne-Laure Boch ◽  
Rémy Guillevin ◽  
...  
Keyword(s):  
High Intensity ◽  
Focused Ultrasound ◽  
High Intensity Focused Ultrasound ◽  
Human Cadaver ◽  
Fresh Cadaver ◽  
Array Transducer ◽  
Human Cadavers ◽  
Ct Data ◽  
The Right ◽  
Human Cadaver Model

Object This work aimed at evaluating the accuracy of MR-guided high-intensity focused ultrasound (MRgHIFU) brain therapy in human cadaver heads. Methods Eighteen heads of fresh human cadavers were removed with a dedicated protocol preventing intracerebral air penetration. The MR images allowed determination of the ultrasonic target: a part of the thalamic nucleus ventralis intermedius implicated in essential tremor. Osseous aberrations were corrected with simulation-based time reversal by using CT data from the heads. The ultrasonic session was performed with a 512-element phased-array transducer system operating at 1 MHz under stereotactic conditions with thermometric real-time MR monitoring performed using a 1.5-T imager. Results Dissection, imaging, targeting, and planning have validated the feasibility of this human cadaver model. The average temperature elevation measured by proton resonance frequency shift was 7.9°C ± 3°C. Based on MRI data, the accuracy of MRgHIFU is 0.4 ± 1 mm along the right/left axis, 0.7 ± 1.2 mm along the dorsal/ventral axis, and 0.5 ± 2.4 mm in the rostral/caudal axis. Conclusions Despite its limits (temperature, vascularization), the human cadaver model is effective for studying the accuracy of MRgHIFU brain therapy. With the 1-MHz system investigated here, there is millimetric accuracy.

scholarly journals Histopathological Features in a Case of Peters' Anomaly with Acquired Corneal Staphyloma

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Kumi Shirai ◽  
Yuka Okada ◽  
Yasushi Nakamura ◽  
Shizuya Saika
Keyword(s):  
Collagen Type ◽  
Corneal Stroma ◽  
Posterior Part ◽  
Collagen Type I ◽  
Type I ◽  
Peters Anomaly ◽  
Hematoxylin And Eosin ◽  
Corneal Staphyloma ◽  
The Right ◽  
Peripheral Cornea

We report a case of corneal staphyloma histologically diagnosed as caused by Peters' anomaly. A 62-year-old male had a protruding opaque vascularized cornea that began to bulge from six months ago in the right eye. Since his right eye was blind and he wanted us to remove the eyeball for cosmetic improvement, we enucleated the affected eye. The enucleated tissue was fixed in formalin and embedded in paraffin for histological examination. Hematoxylin and eosin staining showed that the cornea lacked the posterior part of the corneal stroma and Descemet's membrane in the central region and the entire corneal endothelium. The corneal epithelium was keratinized. Collagen type I was strongly positive in peripheral cornea and weakly in protruding stroma. The cells labeled by antibodies againstαSMA were scattered in the entire corneal stroma. As judged by the histological findings, the eye with the central corneal staphyloma was diagnosed as Peters' anomaly.

scholarly journals Calcium Regulation on the Atrial Regional Difference of Collagen Production Activity in Atrial Fibrogenesis

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 686
Author(s):  
Cheng-Chih Chung ◽  
Yung-Kuo Lin ◽  
Yao-Chang Chen ◽  
Yu-Hsun Kao ◽  
Yung-Hsin Yeh ◽  
...  
Keyword(s):  
Heart Failure ◽  
Transient Receptor Potential ◽  
Collagen Type ◽  
Receptor Potential ◽  
Collagen Type I ◽  
Type I ◽  
Collagen Production ◽  
Type Iii ◽  
Transient Receptor ◽  
The Right

Background: Atrial fibrosis plays an important role in the genesis of heart failure and atrial fibrillation. The left atrium (LA) exhibits a higher level of fibrosis than the right atrium (RA) in heart failure and atrial arrhythmia. However, the mechanism for the high fibrogenic potential of the LA fibroblasts remains unclear. Calcium (Ca2+) signaling contributes to the pro-fibrotic activities of fibroblasts. This study investigated whether differences in Ca2+ homeostasis contribute to differential fibrogenesis in LA and RA fibroblasts. Methods: Ca2+ imaging, a patch clamp assay and Western blotting were performed in isolated rat LA and RA fibroblasts. Results: The LA fibroblasts exhibited a higher Ca2+ entry and gadolinium-sensitive current compared with the RA fibroblasts. The LA fibroblasts exhibited greater pro-collagen type I, type III, phosphorylated Ca2+/calmodulin-dependent protein kinase II (CaMKII), phosphorylated phospholipase C (PLC), stromal interaction molecule 1 (STIM1) and transient receptor potential canonical (TRPC) 3 protein expression compared with RA fibroblasts. In the presence of 1 mmol/L ethylene glycol tetra-acetic acid (EGTA, Ca2+ chelator), the LA fibroblasts had similar pro-collagen type I, type III and phosphorylated CaMKII expression compared with RA fibroblasts. Moreover, in the presence of KN93 (a CaMKII inhibitor, 10 μmol/L), the LA fibroblasts had similar pro-collagen type I and type III compared with RA fibroblasts. Conclusion: The discrepancy of phosphorylated PLC signaling and gadolinium-sensitive Ca2+ channels in LA and RA fibroblasts induces different levels of Ca2+ influx, phosphorylated CaMKII expression and collagen production.

scholarly journals 1054-146 Feasibility of focal septal myocardial ablation through the right ventricle using high-intensity focused ultrasound

2004 ◽  
Vol 43 (5) ◽  
pp. A320
Author(s):  
Kana Fujikura ◽  
Ryo Otsuka ◽  
Todd Pulerwitz ◽  
Takeki Suzuki ◽  
Robert Muratore ◽  
...  
Keyword(s):  
Right Ventricle ◽  
High Intensity ◽  
Focused Ultrasound ◽  
High Intensity Focused Ultrasound ◽  
The Right

The Ferret as a Surgical Model for Vocal Fold Scar Creation and Treatment

2018 ◽  
Vol 127 (3) ◽  
pp. 146-154 ◽  
Author(s):  
Haruka Kodama ◽  
Yoshihiko Kumai ◽  
Kohei Nishimoto ◽  
Yutaka Toya ◽  
Satoru Miyamaru ◽  
...  
Keyword(s):  
Vocal Fold ◽  
Collagen Type ◽  
Experimental Treatment ◽  
Muscle Layer ◽  
Collagen Type I ◽  
Type I ◽  
India Ink ◽  
Vocal Fold Scar ◽  
Female Wistar Rats ◽  
The Right

Objectives: To develop a vocal fold (VF) scarring procedure in the ferret, characterize the scars histologically, and test the injectability of the lamina propria (LP). Secondarily, to compare laryngeal anatomy of the ferret with rat and rabbit. Materials and Methods: The larynges of 18 male ferrets were prepared by unilateral scarring, and normal larynges from 6 female Wistar rats and 5 male albino rabbits were used for comparative purposes. For scarring, the right VF were electrocauterized, ablating the entire LP. Prior to harvesting the larynges at 4 and 16 weeks, each ferret was re-anesthetized, and in 3 animals, India ink was injected into the LPs of both normal and scarred VFs. Results: Laryngoscopic methods and instrumentation for precise visualization, scarring, and injection were developed. The scarred VFs had reduced hyaluronic acid and increased collagen type I, III, and fibronectin compared with normal VFs. The 2 timepoints (4 and 16 weeks) differed significantly only in collagen type III level (levels were higher at 4 weeks). Injected ink migrated from scarred LP to muscle layer just beneath the scarred tissue 3 hours after injection. Conclusion: The ferret is a promising species for creation and experimental treatment of vocal fold scar.

260: High Intensity Focused Ultrasound Combined with Endocrine Therapy in Treating High or Intermediate Risk Prostate Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 86-86
Author(s):  
Makoto Sumitomo ◽  
Junichi Asakuma ◽  
Yasumasa Hanawa ◽  
Kazuhiko Nagakura ◽  
Masamichi Hayakawa
Keyword(s):  
Prostate Cancer ◽  
Endocrine Therapy ◽  
High Intensity ◽  
Focused Ultrasound ◽  
High Intensity Focused Ultrasound ◽  
Intermediate Risk ◽  
Risk Prostate Cancer

1400: Early Clinical Experience Using High-Intensity Focused Ultrasound for the Treatment of Renal Tumours

2005 ◽  
Vol 173 (4S) ◽  
pp. 379-380
Author(s):  
James E. Kennedy ◽  
Rowland O. Illing ◽  
Feng Wu ◽  
Gail R. ter Haar ◽  
Rachel R. Phillips ◽  
...  
Keyword(s):  
Clinical Experience ◽  
High Intensity ◽  
Focused Ultrasound ◽  
High Intensity Focused Ultrasound ◽  
Early Clinical Experience ◽  
Renal Tumours
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