scholarly journals Protein and Metalloprotein Distribution in Different Varieties of Beans (Phaseolus vulgaris L.): Effects of Cooking

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Aline P. Oliveira ◽  
Geyssa Ferreira Andrade ◽  
Bianca S. O. Mateó ◽  
Juliana Naozuka
Keyword(s):  
Phaseolus Vulgaris ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Graphite Furnace ◽  
Total Concentration ◽  
Absorption Spectrometry ◽  
Protein Digestibility ◽  
Black Beans ◽  
Flame Atomic Absorption ◽  
Negative Effect

Beans (Phaseolus vulgaris L.) are among the main sources of protein and minerals. The cooking of the grains is imperative, due to reduction of the effect of some toxic and antinutritional substances, as well as increase of protein digestibility. In this study, the effects of cooking on albumins, globulins, prolamins, and glutelins concentration and determination of Fe associated with proteins for different beans varieties and on phaseolin concentration in common and black beans were evaluated. Different extractant solutions (water, NaCl, ethanol, and NaOH) were used for extracting albumins, globulins, prolamins, and glutelins, respectively. For the phaseolin separation NaOH, HCl, and NaCl were used. The total concentration of proteins was determined by Bradford method; Cu and Fe associated with phaseolin and other proteins were obtained by graphite furnace atomic absorption spectrometry and by flame atomic absorption spectrometry, respectively. Cooking promoted a negative effect on (1) the proteins concentrations (17 (glutelin) to 95 (albumin) %) of common beans and (2) phaseolin concentration (90%) for common and black beans. Fe associated with albumin, prolamin, and glutelin was not altered. In Fe and Cu associated with phaseolin there was an increase of 20 and 37% for the common and black varieties, respectively.

Trace Elements and Metals in Farmed Sea Bass and Gilthead Bream from Tenerife Island, Spain

2011 ◽  
Vol 74 (11) ◽  
pp. 1938-1943 ◽  
Author(s):  
C. RUBIO ◽  
A. JALILLI ◽  
A. J. GUTIÉRREZ ◽  
D. GONZÁLEZ-WELLER ◽  
F. HERNÁNDEZ ◽  
...  
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Sparus Aurata ◽  
Graphite Furnace ◽  
Sea Bass ◽  
Absorption Spectrometry ◽  
Lead And Cadmium ◽  
Flame Atomic Absorption ◽  
Tenerife Island ◽  
Wet Weight

The aim of this study was to determine the levels of metals (Ca, K, Na, Mg) and trace metals (Ni, Fe, Cu, Mn, Zn, Pb, Cd) in two fish species (gilthead bream [Sparus aurata] and sea bass [Dicentrarchus labrax]) collected from fish farms located along the coast of Tenerife Island. Ca, K, Na, Mg, Fe, Cu, Zn, and Mn were measured by flame atomic absorption spectrometry, whereas Pb, Cd, and Ni were determined using graphite furnace atomic absorption spectrometry. Mean Fe, Cu, Mn, and Zn contents were 3.09, 0.59, 0.18, and 8.11 mg/kg (wet weight) in S. aurata and 3.20, 0.76, 0.24, and 10.11 mg/kg (wet weight) in D. labrax, respectively. In D. labrax, Ca, K, Na, and Mg levels were 1,955, 2,787, 699.7, and 279.2 mg/kg (wet weight), respectively; in S. aurata, they were 934.7, 3,515, 532.8, and 262.8 mg/kg (wet weight), respectively. The Pb level in S. aurata was 7.28 ±3.64 μg/kg (wet weight) and, in D. labrax, 4.42 ±1.56 μg/kg (wet weight). Mean Cd concentrations were 3.33 ±3.93 and 1.36 ± 1.53 μg/kg (wet weight) for D. labrax and S. aurata, respectively. All Pb and Cd levels measured were well below the accepted European Commission limits, 300 and 50 μg/kg for lead and cadmium, respectively.

Optimization of Ultrasound-Assisted Extraction of Cr, Cu, Zn, Cd, and Pb from Sediment, Followed by FAAS and GFAAS Analysis

2016 ◽  
Vol 99 (1) ◽  
pp. 252-259 ◽  
Author(s):  
Aparecida M S Mimura ◽  
Marcone A L Oliveira ◽  
Virginia S T Ciminelli ◽  
Julio C J Silva
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Matrix Effects ◽  
Graphite Furnace ◽  
Absorption Spectrometry ◽  
Ultrasound Assisted Extraction ◽  
Flame Atomic Absorption ◽  
Assisted Extraction ◽  
Graphite Furnace Atomic Absorption ◽  
Standard Additions

Abstract An ultrasound method for simultaneous extraction of Cr, Cu, Zn, Cd, and Pb from sediment, and determination by flame atomic absorption spectrometry (FAAS) and graphite furnace atomic absorption spectrometry (GFAAS) was proposed. The experimental results obtained using analytical curves and the method of standard additions agreed at a confidence level of 95% for all the analytes, as determined by FAAS and GFAAS, indicating no significant matrix effects. Recoveries ranged from 80.1 to 93.7% (certified reference material) and from 89 to 107% (spike tests). The LOD and LOQ results from the method were consistent with the techniques used (FAAS and GFAAS), with high analytical throughput. The proposed method was then used to determine Cr, Cu, Zn, Cd, and Pb in river sediment samples from Rio Doce, Minas Gerais, Brazil. The results indicated levels below those permitted by Brazilian legislation for all the analytes, with the exception of Cr.

scholarly journals A comparison of mineral elements content in conventional and organic milk from Southern Poland

2018 ◽  
Vol 114 (1) ◽  
pp. 137-150
Author(s):  
Michał Halagarda ◽  
Joanna Ptasińska-Marcinkiewicz ◽  
Kamil Fijorek
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Graphite Furnace ◽  
Absorption Spectrometry ◽  
Emission Spectrometry ◽  
Cow’S Milk ◽  
Organic Milk ◽  
Cow's Milk ◽  
Southern Poland ◽  
Flame Atomic Absorption

Milk is one of the most important foodstuffs and raw materials in the food industry. As the first complete food available to infant mammals, it is bioactive and it contains all the indispensable nutrients. Organic farming is deemed to produce high quality food under sustainable conditions and, at the same time, to protect the natural environment within the farm. However, the most recent food and nutrition research does not confirm the extensive health benefits related to the consumption of organic products. The objective of the research study was to evaluate and compare the quality of commercial organic and conventional cow’s milk as regards the contents of some selected mineral compounds. The research was conducted on the organic and conventional cow’s milk available on the market in Southern Poland. The milk samples were analysed for the concentration of selected minerals contained therein, including some selected toxic metals; the analyses were performed with the use of flame atomic absorption spectrometry, atomic emission spectrometry, and graphite furnace atomic absorption spectrometry. The results of the research show that, in terms of the contents of micro- and macroelements, the organic and conventional milk do not differ significantly. The differences were found only between the amounts of sodium and manganese. The organic milk contained, on average, a statistically significantly smaller amount of those elements. Moreover, the presence of lead was detected in one type of the organic milk, although its amount determined (5.24 mg/l) was within the acceptable limits. Cadmium (amounting to 0.12 and 0.15 μg/l) was found in the two types of milk derived from the same company and in one type of organic milk; however, its concentration in the latter milk type was at a relatively low level (0.04 μg/l).

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