scholarly journals Changes in Nitric Oxide Releases of the Contralateral Acupoint during and after Laser Acupuncture at Bilateral Same-Name Acupoints in Human

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Wan-Ling Jiang ◽  
Hua-Jiang Wei ◽  
Zhou-Yi Guo ◽  
Yi-Rong Ni ◽  
Hong-Qin Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Healthy Subjects ◽  
Vessel Wall ◽  
Time Course ◽  
Contralateral Side ◽  
Control Area ◽  
Laser Acupuncture ◽  
Blinded Fashion ◽  
No Release ◽  
No Releases

Objective.The purpose of the study was to examine the effects of laser acupuncture (LA) at right Neiguan (RPC6)/left Neiguan (LPC6) acupoints on the releases of nitric oxide (NO) in the treated and contralateral/nontreated PC6, compared to the nonacupoint control area.Methods. 24 mW LA at RPC6, LPC6, and nonacupoint in 22 healthy subjects for 40 min: sterilized dialysis tube was taped to the nontreated PC6/nonacupoint during the treatment and immediately taped to the treated and nontreated PC6/nonacupoint after LA removal. NO-scavenging compound was injected into the tube for 40 min to absorb the molecular which was tested by spectrophotometry in a blinded fashion.Results. LA-induced NO releases over PC6 acupoints for the nontreated and treated sides all significantly increased after LA removal, but for the nontreated acupoints they did not change during LA stimulation. LA at RPC6 induced the more release of the NO at contralateral side than stimulating LPC6, but not on nonacupoints. The results suggest that LA-induced NO release over contralateral acupoint and NO release resulting from the lateralized specificity all are different and specific to the acupoint within different time course.Conclusions. LA-evoked NO release over acupoints could improve the neurogenic, endothelial activity of the vessel wall to further facilitate microcirculation.

scholarly journals Response of Local Nitric Oxide Release to Manual Acupuncture and Electrical Heat in Humans: Effects of Reinforcement Methods

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Sheng-Xing Ma ◽  
Paul C. Lee ◽  
Thomas L. Anderson ◽  
Xi-Yan Li ◽  
Isabelle Z. Jiang
Keyword(s):  
Nitric Oxide ◽  
Skin Surface ◽  
Manual Acupuncture ◽  
Local Circulation ◽  
Local Skin ◽  
Nitric Oxide Release ◽  
Beneficial Effects ◽  
Blinded Fashion ◽  
No Release

This study was to examine the influences of manual acupuncture (MA) and electrical heat corresponding to reinforcing methods on nitric oxide (NO) release over the skin regions in humans. A device with collecting solution was taped to the skin surface along pericardium (PC) or lung (LU) meridian. Acupuncture needles were gently inserted into PC 4 with reinforcing stimulation (low force/rate) for 20 minutes in the MA group. LU11 on the finger was heated (43-44°C) by electrical heat for 20 minutes. Biocapture was consecutively conducted for two 20-minute intervals during and after each treatment. Total nitrite and nitrate (NOx-) in the collecting samples were quantified using chemiluminescence in blinded fashion. BaselineNOx-levels are higher and tended to be higher over PC and LU acupoints during the 1st biocapture.NOx-levels over PC regions were consistently increased by MA during both intervals.NOx-concentrations over LU acupoints were increased and tended to be increased by electrical heat in the 1st and 2nd biocapture. The results suggest that reinforcing MA and electrical heat induce NO released from the local skin regions with higher levels at acupoints, which improve local circulation and contribute to the beneficial effects of the therapies.

Advective transport of nitric oxide in a mathematical model of the afferent arteriole

2003 ◽  
Vol 284 (5) ◽  
pp. F1080-F1096 ◽  
Author(s):  
Kayne M. Smith ◽  
Leon C. Moore ◽  
Harold E. Layton
Keyword(s):  
Nitric Oxide ◽  
Mathematical Model ◽  
Transit Time ◽  
Vessel Wall ◽  
Transport Model ◽  
Afferent Arteriole ◽  
Advective Transport ◽  
Binding Rate ◽  
No Release ◽  
Rapid Removal

Endothelium-derived nitric oxide (NO) is thought to be short-lived in blood because of rapid removal from plasma, mainly by binding to Hb. The extent to which removal limits NO advection is unclear, especially for blood flow in the renal afferent arteriole (AA), which has a transit time of 3–30 ms. A mathematical model of AA fluid dynamics and myogenic response that includes NO diffusion, advection, degradation, and vasorelaxant action was used to estimate NO advective transport. Model simulations indicate that advective transport of locally produced NO is sufficient to yield physiologically significant NO concentrations along much of the AA. Advective transport is insensitive to NO scavenging by Hb because the NO-Hb binding rate is slow relative to AA transit time. Hence, plasma NO concentration near the vessel wall is influenced by both diffusion from endothelial cells and advection from upstream sites. Simulations also suggest that NO advection may constitute a mechanism to stabilize arteriolar flow in response to a localized vasoconstriction accompanied by enhanced NO release.

Transcutaneous electrical stimulation applied to the infratrochlear nerve induces a homatropine-resistant miosis in humans

1990 ◽  
Vol 78 (5) ◽  
pp. 457-462 ◽  
Author(s):  
B. M. Fusco ◽  
M. Alessandri ◽  
V. Campagnolo ◽  
M. Fanciullacci
Keyword(s):  
Electrical Stimulation ◽  
High Intensity ◽  
Healthy Subjects ◽  
Time Course ◽  
Contralateral Side ◽  
Pupillary Response ◽  
Transcutaneous Electrical Stimulation ◽  
Antidromic Activation ◽  
Light Reflex ◽  
Low Intensity

1. Both high- and low-intensity transcutaneous electrical stimuli were applied to the emergence of the infratrochlear nerve in 18 healthy subjects. The effect on the size of the homolateral pupil was investigated. The width of the pupil was also measured when high-intensity transcutaneous electrical stimulation was applied to the contralateral side. 2. The high-intensity pulse resulted in constriction of the pupil when the stimulation was homolateral. The miosis was slow in onset (120 s latency) and long-lasting (80 s). No pupillary changes were detected after either ipsilateral low-intensity or contralateral high-intensity stimuli. 3. In 11 healthy subjects, the pupillary response to transcutaneous electrical stimulation was evaluated during iris parasympathetic blockade induced by homatropine eyedrops. The disappearance of the light reflex due to homatropine was considered an index of the parasympathetic blockade. Afterwards, a high-intensity pulse was transcutaneously delivered to the emergence of the infratrochlear nerve and the ipsilateral pupil size was measured. 4. A reduction in the pupillary size followed the electrical stimulation, still under the effect of homatropine which abolished the light reflex. The time course of this pupillary constriction was similar to that seen without the influence of homatropine. 5. The findings suggest that homolateral miosis, observed after unilateral high-intensity stimulation of the infratrochlear nerve, does not stem from cholinergic activation. It has been suggested that miosis induced by transcutaneous electrical stimulation may be due to an antidromic activation of the iris sensory fibres.

Decrease in ambient [Cl-] stimulates nitric oxide release from cultured rat mesangial cells

1994 ◽  
Vol 267 (1) ◽  
pp. F190-F195 ◽  
Author(s):  
H. Tsukahara ◽  
Y. Krivenko ◽  
L. C. Moore ◽  
M. S. Goligorsky
Keyword(s):  
Nitric Oxide ◽  
Mesangial Cells ◽  
Ionic Composition ◽  
Juxtaglomerular Apparatus ◽  
Cytosolic Calcium ◽  
No Synthase ◽  
Tubuloglomerular Feedback ◽  
No Production ◽  
Rapid Reduction ◽  
No Release

It has been hypothesized that fluctuations of the ionic composition in the interstitium of juxtaglomerular apparatus (JGA) modulate the function of extraglomerular mesangial cells (MC), thereby participating in tubuloglomerular feedback (TGF) signal transmission. We examined the effects of isosmotic reductions in ambient sodium concentration ([Na+]) and [Cl-] on cytosolic calcium concentration ([Ca2+]i) in cultured rat MC. Rapid reduction of [Na+] or [Cl-] in the bath induced a concentration-dependent rise in [Ca2+]i. MC are much more sensitive to decreases in ambient [Cl-] than to [Na+]; a decrease in [Cl-] as small as 14 mM was sufficient to elicit a detectable [Ca2]i response. These observations suggest that MC can be readily stimulated by modest perturbations of extracellular [Cl-]. Next, we examined whether activation of MC by lowered ambient [Cl-] influences cellular nitric oxide (NO) production. Using an amperometric NO sensor, we found that a 13 mM decrease in ambient [Cl-] caused a rapid, Ca2+/calmodulin-dependent rise in NO release from MC. This response was not inhibitable by dexamethasone, indicating the involvement of the constitutive rather than the inducible type of NO synthase in MC. In addition, the NO release was blunted by indomethacin pretreatment, suggesting that a metabolite(s) of cyclooxygenase regulates the activation of NO synthase in MC. Our findings that small perturbations in external [Cl-] stimulate MC to release NO, a highly diffusible and rapidly acting vasodilator, provide a possible mechanism to explain the transmission of the signal for the TGF response within the JGA.

scholarly journals Time Course of Inducible Nitric Oxide Synthase Activity Following Endotoxin Administration in Dogs

Nitric Oxide ◽  
2001 ◽  
Vol 5 (2) ◽  
pp. 208-211 ◽  
Author(s):  
Jean-Charles Preiser ◽  
Haibo Zhang ◽  
Bernard Vray ◽  
Andreas Hrabak ◽  
Jean-Louis Vincent
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Time Course ◽  
Endotoxin Administration ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity ◽  
Inducible Nitric Oxide

Retracted Article: Light-triggered nitric oxide release and targeted fluorescence imaging in tumor cells developed from folic acid-graft-carboxymethyl chitosan nanospheres

RSC Advances ◽  
2014 ◽  
Vol 4 (57) ◽  
pp. 30129-30136 ◽  
Author(s):  
Rijun Gui ◽  
Ajun Wan ◽  
Yalei Zhang ◽  
Huili Li ◽  
Tingting Zhao
Keyword(s):  
Nitric Oxide ◽  
Folic Acid ◽  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Carboxymethyl Chitosan ◽  
Nitric Oxide Release ◽  
No Release ◽  
Retracted Article ◽  
Potential Applications

This article reported the synthesis of CMC–FA–RBS(CQD) nanospheres and studied their potential applications for NO release and fluorescence imaging.

Recovery of subjective visual horizontal after unilateral vestibular deafferentation by intratympanic instillation of gentamicin

2006 ◽  
Vol 16 (1-2) ◽  
pp. 69-73 ◽  
Author(s):  
Yoshinari Takai ◽  
Toshihisa Murofushi ◽  
Munetaka Ushio ◽  
Shinichi Iwasaki
Keyword(s):  
Healthy Subjects ◽  
Time Course ◽  
Early Stage ◽  
Ccd Camera ◽  
The Other ◽  
Spontaneous Nystagmus ◽  
Vestibular Evoked Myogenic Potentials ◽  
Normal Range ◽  
Intratympanic Gentamicin ◽  
Unilateral Vestibular Deafferentation

The time course of the recovery of subjective visual horizontal (SVH) after unilateral vestibular deafferentation by intratympanic instillation of gentamicin was studied. Six patients who underwent intratympanic gentamicin instillation therapy for Meniere's disease (1 man and 5 women, 32 to 69 years of age) were enrolled in this study. For comparison, SVH in 23 healthy subjects (12 men and 11 woman, 23 to 48 years of age) was also measured. The mean ± SD of SVH in healthy subjects was 0.0 ± 1.1 deg. All of the 6 patients showed significantly deviated SVH toward the injected side-down at the early stage after the therapy. Although one patient showed recovery of SVH to the normal range 25 days after the injection, the other patients required more time for recovery. Three patients did not show recovery to the normal range after 1 year. On the other hand, spontaneous nystagmus observed using an infrared CCD camera in total dark disappeared after 35 days (median). Patients who had normal vestibular evoked myogenic potentials before the therapy showed a tendency of delay of recovery of SVH. The reasons why the recovery of SVH took longer than the disappearance of spontaneous nystagmus are discussed in this report.

Hollow double-layered polymer nanoparticles with S-nitrosothiols for tumor targeted therapy

2017 ◽  
Vol 5 (36) ◽  
pp. 7519-7528 ◽  
Author(s):  
Tuanwei Liu ◽  
Jingjing Hu ◽  
Xiaoye Ma ◽  
Bing Kong ◽  
Jilan Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Targeted Therapy ◽  
Polymer Nanoparticles ◽  
No Release

Tumor targeted hollow double-layered polymer nanoparticles (HDPNs) withS-nitrosothiols for nitric oxide (NO)-release as chemotherapy were described.

Low nitric oxide concentrations in exhaled gas and nasal airways of mammals without paranasal sinuses

1998 ◽  
Vol 85 (2) ◽  
pp. 405-410 ◽  
Author(s):  
Klaus Lewandowski ◽  
Thilo Busch ◽  
Hansjörg Lohbrunner ◽  
Susanne Rensing ◽  
Uwe Keske ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Paranasal Sinuses ◽  
Ambient Air ◽  
Face Mask ◽  
Plastic Tube ◽  
Nasal Airways ◽  
No Release ◽  
Age Dependent ◽  
Thin Plastic ◽  
Expiratory Limb

To investigate whether relevant levels of nasal nitric oxide (NO) are produced in the absence of paranasal sinuses, we studied 17 healthy baboons, mammals without any paranasal sinuses. The animals were anesthetized with ketamine hydrochloride and breathed spontaneously. While the baboons breathed through a face mask (mouths closed) connected to a respirator, NO concentrations in exhaled gas were sampled from the expiratory limb and analyzed by chemiluminescence. While the animals were breathing ambient air, nasal gas was sampled via a thin plastic tube and analyzed for NO concentrations by chemiluminescence. Mean NO concentration in the exhaled gas was 1.00 ± 0.59 parts/billion, and NO release was 4.28 ± 2.72 nl/min. A NO concentration of 4.79 ± 2.08 parts/billion was found in the nasal gas (NO release: 7.18 ± 3.13 nl/min). An age-dependent increase in nasal NO levels was not observed. Exhaled and nasal NO concentrations in baboons were markedly lower than in mammals with paranasal sinuses, suggesting that paranasal sinuses might be an anatomic requirement for production of relevant nasal NO levels.

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