scholarly journals Exploiting Microwave Imaging Methods for Real-Time Monitoring of Thermal Ablation

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Rosa Scapaticci ◽  
Gennaro G. Bellizzi ◽  
Marta Cavagnaro ◽  
Vanni Lopresto ◽  
Lorenzo Crocco
Keyword(s):  
Real Time ◽  
Thermal Ablation ◽  
Ex Vivo ◽  
Local Heating ◽  
Biological Tissues ◽  
Microwave Imaging ◽  
Electromagnetic Properties ◽  
Potential Candidate ◽  
Real Time Monitoring ◽  
Concept Validation

Microwave thermal ablation is a cancer treatment that exploits local heating caused by a microwave electromagnetic field to induce coagulative necrosis of tumor cells. Recently, such a technique has significantly progressed in the clinical practice. However, its effectiveness would dramatically improve if paired with a noninvasive system for the real-time monitoring of the evolving dimension and shape of the thermally ablated area. In this respect, microwave imaging can be a potential candidate to monitor the overall treatment evolution in a noninvasive way, as it takes direct advantage from the dependence of the electromagnetic properties of biological tissues from temperature. This paper explores such a possibility by presenting a proof of concept validation based on accurate simulated imaging experiments, run with respect to a scenario that mimics an ex vivo experimental setup. In particular, two model-based inversion algorithms are exploited to tackle the imaging task. These methods provide independent results in real-time and their integration improves the quality of the overall tracking of the variations occurring in the target and surrounding regions.

scholarly journals TDOA-based microwave imaging algorithm for real-time microwave ablation monitoring

2017 ◽  
Vol 10 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Shouhei Kidera ◽  
Luz Maria Neira ◽  
Barry D. Van Veen ◽  
Susan C. Hagness
Keyword(s):  
Real Time ◽  
Microwave Ablation ◽  
Microwave Imaging ◽  
Ablation Zone ◽  
Real Time Monitoring ◽  
Imaging Algorithm ◽  
Array Measurements ◽  
Time Operation ◽  
Real Time Operation ◽  
Propagation Media

Microwave ablation is widely recognized as a promising minimally invasive tool for treating cancer. Real-time monitoring of the dimensions of the ablation zone is indispensable for ensuring an effective and safe treatment. In this paper, we propose a microwave imaging algorithm for monitoring the evolution of the ablation zone. Our proposed algorithm determines the boundary of the ablation zone by exploiting the time difference of arrival (TDOA) between signals received before and during the ablation at external antennas surrounding the tissue, using the interstitial ablation antenna as the transmitter. A significant advantage of this method is that it requires few assumptions about the dielectric properties of the propagation media. Also the simplicity of the signal processing, wherein the TDOA is determined from a cross-correlation calculation, allows real-time monitoring and provides robust performance in the presence of noise. We investigate the performance of this approach for the application of breast tumor ablation. We use simulated array measurements obtained from finite-difference time-domain simulations of magnetic resonance imaging-derived numerical breast phantoms. The results demonstrate that our proposed method offers the potential to achieve millimeter-order accuracy and real-time operation in estimating the boundary of the ablation zone in heterogeneous and dispersive breast tissue.

scholarly journals Ex vivo real-time monitoring of volatile metabolites resulting from nasal odorant metabolism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aline Robert-Hazotte ◽  
Rachel Schoumacker ◽  
Etienne Semon ◽  
Loïc Briand ◽  
Elisabeth Guichard ◽  
...  
Keyword(s):  
Real Time ◽  
Ex Vivo ◽  
Real Time Monitoring ◽  
Volatile Metabolites

scholarly journals Publisher Correction: Ex vivo real-time monitoring of volatile metabolites resulting from nasal odorant metabolism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aline Robert-Hazotte ◽  
Rachel Schoumacker ◽  
Etienne Semon ◽  
Loïc Briand ◽  
Elisabeth Guichard ◽  
...  
Keyword(s):  
Real Time ◽  
Ex Vivo ◽  
Real Time Monitoring ◽  
Volatile Metabolites

scholarly journals Monitoring Thermal Ablation via Microwave Tomography: An Ex Vivo Experimental Assessment

Diagnostics ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 81 ◽  
Author(s):  
Rosa Scapaticci ◽  
Vanni Lopresto ◽  
Rosanna Pinto ◽  
Marta Cavagnaro ◽  
Lorenzo Crocco
Keyword(s):  
Thermal Ablation ◽  
Ex Vivo ◽  
Imaging Techniques ◽  
Bovine Liver ◽  
Electromagnetic Properties ◽  
Microwave Tomography ◽  
Temperature Dependent ◽  
Discussion Section ◽  
Monitoring Technique ◽  
Significant Temperature

Thermal ablation treatments are gaining a lot of attention in the clinics thanks to their reduced invasiveness and their capability of treating non-surgical patients. The effectiveness of these treatments and their impact in the hospital’s routine would significantly increase if paired with a monitoring technique able to control the evolution of the treated area in real-time. This is particularly relevant in microwave thermal ablation, wherein the capability of treating larger tumors in a shorter time needs proper monitoring. Current diagnostic imaging techniques do not provide effective solutions to this issue for a number of reasons, including economical sustainability and safety. Hence, the development of alternative modalities is of interest. Microwave tomography, which aims at imaging the electromagnetic properties of a target under test, has been recently proposed for this scope, given the significant temperature-dependent changes of the dielectric properties of human tissues induced by thermal ablation. In this paper, the outcomes of the first ex vivo experimental study, performed to assess the expected potentialities of microwave tomography, are presented. The paper describes the validation study dealing with the imaging of the changes occurring in thermal ablation treatments. The experimental test was carried out on two ex vivo bovine liver samples and the reported results show the capability of microwave tomography of imaging the transition between ablated and untreated tissue. Moreover, the discussion section provides some guidelines to follow in order to improve the achievable performances.

Quantitative magnetic resonance temperature mapping for real-time monitoring of radiofrequency ablation of the liver: an ex vivo study

2006 ◽  
Vol 16 (10) ◽  
pp. 2265-2274 ◽  
Author(s):  
Olivier Seror ◽  
Matthieu Lepetit-Coiffé ◽  
Bruno Quesson ◽  
Hervé Trillaud ◽  
Chrit T W Moonen
Keyword(s):  
Radiofrequency Ablation ◽  
Magnetic Resonance ◽  
Real Time ◽  
Ex Vivo ◽  
Real Time Monitoring ◽  
Temperature Mapping ◽  
Quantitative Magnetic Resonance ◽  
Ex Vivo Study

Imaging Guided Percutaneous Irreversible Electroporation: Ultrasound and Immunohistological Correlation

2007 ◽  
Vol 6 (4) ◽  
pp. 287-293 ◽  
Author(s):  
Edward W. Lee ◽  
Christopher T. Loh ◽  
Stephen T. Kee
Keyword(s):  
Cell Death ◽  
Real Time ◽  
Thermal Ablation ◽  
Apoptotic Cell ◽  
Irreversible Electroporation ◽  
Apoptotic Cell Death ◽  
Real Time Monitoring ◽  
Apoptotic Marker ◽  
Swine Liver ◽  
Mean Size

Preliminary results of percutaneous irreversible electroporation (PIE) on swine liver as a novel non-thermal ablation are presented. The goal of this study was to evaluate the feasibility of using irreversible electroporation in more clinically applicable manner, a percutaneous method, and to investigate a possible role of apoptosis in PIE-induced cell death. We performed PIE on four swine livers under real-time ultrasound guidance. The lesions created by PIE were imaged with ultrasound and were correlated with histology data, including pro-apoptotic marker. A total of 11 lesions were created with a mean size of 16.8 cm3 in 8.4 ± 1.8 minutes. Real-time monitoring was performed and a correlation of (+) 2 ± 3.2 mm in measurement comparison between ultrasound and gross pathologic measurements was demonstrated. Complete hepatic cell death without structural destruction, unaffected by heat-sink effect, and with a sharp demarcation between the ablated zone and the non-ablated zone were observed. Immunohistological analysis confirmed complete apoptotic cell death by PIE on Von Kossa, BAX, and H&E staining. In summary, PIE can provide a novel and unique ablative method with real-time monitoring capability, ultra-short procedure time, non-thermal ablation, and well-controlled and focused apoptotic cell death.

scholarly journals Real-time monitoring of mesangial cell-macrophage cross-talk using SEAP in vitro and ex vivo

2005 ◽  
Vol 68 (2) ◽  
pp. 886-893 ◽  
Author(s):  
Yiman Meng ◽  
Ayumi Kasai ◽  
Nobuhiko Hiramatsu ◽  
Kunihiro Hayakawa ◽  
Masayuki Takeda ◽  
...  
Keyword(s):  
Real Time ◽  
Cross Talk ◽  
Mesangial Cell ◽  
Ex Vivo ◽  
Real Time Monitoring

P6308Use of cell-embedded fully implantable biosensor system for real-time, in-vivo monitoring of NF-Kappa B inflammatory pathway

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
S Rosero ◽  
F Machet ◽  
M Kowarz ◽  
E Brown ◽  
J Lopes-Vicente ◽  
...  
Keyword(s):  
Real Time ◽  
Cellular Response ◽  
Time Course ◽  
Ex Vivo ◽  
Recovery Period ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Pathway Activation ◽  
Biosensor System

Abstract Background 21st century health care remains anchored to sporadic measurements of traditional physiologic variables such as heart rate, blood pressure, weight, physical exam findings and general biochemical values. Currently there is no sensor capable of monitoring in intra-cellular protein and gene level signaling in real-time and in-vivo. This gap between biological signaling and its translation into clinically relevant therapeutics targeting the individual has limited precision medicine approaches to heart and vascular diseases. Inflammatory processes have been implicated in numerous cardiovascular diseases providing an ideal target for using Biologically based-Implantable Electronic Devices (BIED) approaches. Objective We aimed to test an implantable electro-photonic biosensor system in which living cells are integrated within the BIED and 1) serve as the primary sensor element providing in-vivo, real-time monitoring of intra-cellular processes such as gene expression, protein signaling, and target pathway activation, and 2) provide intelligent biologically based-processing in which the the output reflects biological response to an event. The engineered sensor cells provide real-time monitoring and respond to prespecified biologic signals using green fluorescence protein (GFP). The fluorescence is then detected via the BIED's photonic system and the cellular response data transmitted providing remote monitoring capabilities to facilitate the development of innovative personalized therapeutics. Methods A biologically-based implantable biosensor (BIED) platform which provides fluorescence detection, data acquisition and transmission from living cells integrated within the device was tested. The sensor cells communicate with the surrounding implant environment via a biomembrane. NRK rat cells engineered to express GFP in response to NF-κB pathway activation were cultured and housed within the sensor. Prior implant studies confirmed NRK sensor cells remained viable for 21 days in-vivo as part of a fully functional implanted BIED hardware system. Ex-vivo experiments characterized the expected magnitude and time course of NRK response to TNF-α and Lipopolysaccharide (LPS) exposure used to elicit a proinflammatory inflammatory response. The biosensor was implanted in the subcutaneous space of male Sprague Dawley rats (n=2) for a total of 11 days consisting of a baseline post-surgical recovery period of 7 days, with subsequent challenge with intraperitoneal LPS on Day 8 and 96 hours of post LPS monitoring. Results Rats implanted with the Biological based sensor and challenged with intraperitoneal LPS showed real-time expression of GFP under NF-κB transcriptional control following time course similar to ex-vivo experiments (p<0.05) (Figure 1). Figure 1. Implantable Cell-Embedded Biosensor Conclusion We present the first in-vivo use of a new class of BIEDs to detect biological cell response which may herald real-time personalize health management at the molecular and cellular level. Acknowledgement/Funding Clinical and Translational Sciences Institute-University of Rochester, Efferent Labs

scholarly journals Fiber Optic Sensor for Real-time Monitoring of Freezing–Thawing Cycle in Cryosurgery

2020 ◽  
Vol 10 (3) ◽  
pp. 1053
Author(s):  
Dimosthenis Spasopoulos ◽  
George Rattas ◽  
Archontis Kaisas ◽  
Thomas Dalagiannis ◽  
Ioannis D. Bassukas ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Transmission ◽  
Fiber Optic ◽  
Expert Knowledge ◽  
Ex Vivo ◽  
Fiber Optic Sensor ◽  
Real Time Monitoring ◽  
Fiber Array ◽  
Optic Sensor ◽  
Optical Fiber Array

Cryosurgery/cryotherapy is a widely used, freezing–thawing technique for the renewal or destruction of pathological tissues by applying localized rapid cooling; however, it still relies on the subjective “expert knowledge” of the physicians without, up to now, real-time monitoring of the treatment. This work focused on assessing the depth of freezing using optical transmission and backscattering measurements from frozen/unfrozen porcine ex-vivo skin samples. An optical fiber-array sensor was subsequently developed to determine the depth of freezing and the associated kill zone during freeze–thawing cycles with sub-millimeter accuracy within the skin tissue.

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