scholarly journals Development of a Sauce Using Gymnema sylvestre Leaves

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
O. D. A. N. Perera ◽  
P. Pavitha
Keyword(s):  
Sodium Benzoate ◽  
Bitter Taste ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Gymnema Sylvestre ◽  
Flavonoid Content ◽  
Diverse Range ◽  
Total Antioxidant ◽  
Therapeutic Properties ◽  
Gallic Acid Equivalent

Plant derived products have attracted enormous attention due to their diverse range of biological and therapeutic properties. Gymnema sylvestre is an underutilized medicinal herb that is widely distributed in different parts of the world. It is rich in phytochemicals with antidiabetic and antiobesity properties; however, it is less popular due to its bitter taste. This study was focused on developing a sauce using leaves of Gymnema sylvestre. The most acceptable composition (17.2% water, 4.8% vinegar, 3.2% sweet potato, 2.6% chili, 2.0% sugar, 2.0% cinnamon, 2.0% cardamom, 1.8% ginger, 1.8% garlic, 1.2% onion, 1.2% salt, 0.1% citric acid, and 0.1% sodium benzoate) of the sauce was with 60% leaf extract. Final product contains 73.54% ± 1.68 moisture, 19.92% ± 2.59 carbohydrate, 2.98% ± 0.44 ash, 2.61% ± 0.58 protein, 0.88% ± 0.05 fiber, and 0.05% ± 0.01 fat. The total flavonoid content was 243.67 ± 8.57 µ moles of rutin equivalent, total phenolic content was 65.44 ± 0.51 µ moles of gallic acid equivalent, and % of inhibition of DPPH of total antioxidant capacity was 43.70 ± 0.72. Sauce formulated with 1000 ppm sodium benzoate had six weeks of shelf life. The results showed that development of a sauce from G. sylvestre leaves is an effective way of delivering the health benefits to the potential consumers.

scholarly journals Proximate and Biological Activity Studies of Syzygium samarangense: Study of different extractives of Syzygium samarangense

2021 ◽  
Vol 3 (1) ◽  
pp. 172-177
Author(s):  
Mohammad Shoeb ◽  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ash Content ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Methanolic Extracts ◽  
Total Antioxidant ◽  
Polycyclic Aromatic ◽  
Red Variety

Fruits of red and green varieties of Syzygium samarangense, commonly known as Jamrul in Bangladesh, are very popular for all ages of people. Air-dried fruits of S. samarangense were successively extracted with n-hexane, dichloromethane (DCM) and methanol. The moisture and ash content of the raw fruits were determined by thermal heating at 105 and 700, respectively. Different biologically important functional groups were identified by FT-IR spectroscopy. The n-hexane, DCM and methanolic extracts were tested for cytotoxicity against HeLa and Vero cell lines. Total phenolic content, total flavonoid content and total antioxidant capacity in the three extracts of both varieties were determined. The moisture content and ash content were 92% and 90%, and 0.12% and 0.16% in red variety and green variety, respectively. UV-Vis spectrum revealed the presence of long chain conjugation or polycyclic aromatic chromophores in n-hexane, DCM and methanolic extracts of S. samarangense. The DCM extract of both varieties had highest total phenolic content, flavonoid content and antioxidant capacity compared to n-hexane and methanol extracts. The study concludes that the fruit of S. samarangense (red and green variety) grown in Bangladesh are rich source of phytochemicals which possess antioxidant activity and safe to health for their non-cytotoxic properties. The green variety contains more phenolic content and antioxidant capacity than red variety.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

scholarly journals Optimization of Uruset Apple Vinegar Production Using Response Surface Methodology for the Enhanced Extraction of Bioactive Substances

Foods ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 107 ◽  
Author(s):  
Seydi Yıkmış
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Process Conditions ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Bioactive Substances ◽  
Total Antioxidant

In this study, the aim is to produce non-thermal vinegar by using red Uruset apples, which have high bioavailability among apple varieties. For this purpose, Uruset apple vinegar was produced and ultrasound at different times (2, 4, 6, 8 and 10 min) and different amplitudes (40%, 50%, 60%, 70%, and 80%); in addition, a 26 kHz frequency was applied to the samples. Total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (1,1-diphenyl- 2-picrylhydrazyl (DPPH) and cupric reducing antioxidant capacity (CUPRAC)), and color values were evaluated for the optimization of process conditions. At the same time, the differences between commercial apple vinegar (CV), pasteurized Uruset apple vinegar (PV), and a control (C) of untreated apple vinegar were investigated. Ultrasound treatment of Uruset apple vinegar was more successful for the enrichment of bioactive substances than the other samples. At the end of the study, the maximal optimization values for Uruset apple vinegar were 7.4 min and 62.2 amplitude. At the end of optimization, CUPRAC (0.69 mg TEAC/mL), DPPH (0.49 mg TEAC/mL), total flavonoid content (46.95 mg CE/L), and total phenolic content (124.25 mg GAE/L) were determined. As a result, ultrasound technology was successfully used for Uruset apple vinegar production.

Phenolic Profile and Antioxidant Capacity of Walnut Extract as Influenced by the Extraction Method and Solvent

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Ion Trandafir ◽  
Sina Cosmulescu ◽  
Violeta Nour
Keyword(s):  
Dry Matter ◽  
Phenolic Content ◽  
Soxhlet Extraction ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic Contents ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Gallic Acid Equivalent

Abstract Total phenolic content, total flavonoid content, antioxidant activity and individual phenolic compounds were assessed in full fat and defatted walnut kernel. For quantification of phenolic fraction of walnut kernels, two different solvents (methanol and ethanol) and two methods of extraction (ultrasonic-assisted extraction and Soxhlet extraction) were tested. Total phenolics, flavonoid content and antioxidant capacity of alcoholic extracts varied depending on the solvent used and extraction methods. Seventeen phenolic compounds were detected and the study provides evidence on high phenolic contents and high antioxidant potential of full fat walnut kernel and defatted walnut kernel. The Soxhlet extraction is the best in terms of the amounts of total phenolic content (2,089.2 mg gallic acid equivalent/100 g dry matter), while the ultrasonic assisted extraction is a fast method but resulted in significantly lower phenolic content (667.3–1,426.8 mg gallic acid equivalent /100 g dry matter). The concentrations of phenolics (especially (+)-catechin hydrate, juglone, vanillic acid, caffeic acid, ferulic acid, sinapic acid, salicylic acid and ellagic acid) are many fold lower in ultrasonic-assisted extraction as compare to the Soxhlet method using the same extraction solvent. The results of this study provide evidence on high phenolic contents and high antioxidant potential of full fat and defatted walnut kernel.

scholarly journals UV-B Radiation as Abiotic Elicitor to Enhance Phytochemicals and Development of Red Cabbage Sprouts

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 567
Author(s):  
Lorena Martínez-Zamora ◽  
Noelia Castillejo ◽  
Francisco Artés-Hernández
Keyword(s):  
Phenolic Content ◽  
Total Phenolic ◽  
Morphological Development ◽  
Flavonoid Content ◽  
Hypocotyl Length ◽  
Red Cabbage ◽  
Total Antioxidant ◽  
Abiotic Elicitor ◽  
Dose Dependent ◽  
Dependent Behaviour

Background: The main objective of this study was to evaluate the effect of periodical UV-B illumination during red cabbage germination on morphological development and the phenolics and carotenoid accumulation. Methods: During a sprouting period of 10 days at 20 °C in darkness, seedlings received 5, 10, or 15 kJ m−2 UV-B (T5, T10, and T15) applied in four steps (25% on days 3, 5, 7, and 10). UV untreated sprouts were used as control (CTRL). After 10 days of germination, the sprouts were harvested and stored 10 days at 4 °C as a minimally processed product. Phenolic and carotenoid compounds were analysed 1 h after each UV-B application and on days 0, 4, 7, and 10 during cold storage. Results: The longest hypocotyl length was observed in T10-treated sprouts. The total phenolic content (TPC), total flavonoid content (TFC), and total antioxidant capacity (TAC) increased during germination following a sigmoidal kinetic, especially in the UV-B-treated samples, which reported a dose-dependent behaviour. In this way, T10-treated sprouts increased the TPC by 40% after 10 days at 4 °C compared to CTRL, while TAC and TFC increased by 35 and 30%, respectively. Carotenoids were enhanced with higher UV-B doses (T15). Conclusions: We found that UV-B stimulated the biosynthesis of bioactive compounds, and a dose of 10 kJ m−2 UV-B, proportionally applied on days 3, 5, 7, and 10 days, is recommended.

scholarly journals The Comparison of Total Phenolics, Total Antioxidant, and Anti-Tyrosinase Activities of Korean Sargassum Species

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Su Hyeon Baek ◽  
Lei Cao ◽  
Seung Jin Jeong ◽  
Hyeung-Rak Kim ◽  
Taek Jeung Nam ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Health Benefits ◽  
Dry Weight ◽  
Tyrosinase Activity ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Antioxidant Capacities ◽  
Total Antioxidant ◽  
Gallic Acid Equivalent ◽  
Phenolic And Flavonoid

Sargassum species, a group of marine brown algae consumed in Asian countries, have shown various health benefits, such as improving the conditions of cardiovascular disease, osteoarthritis, and hypopigmentation. Also, these benefits are associated with their phenolic content and strong antioxidant capacities. However, the antioxidant capacities of different Sargassum species had not been thoroughly explored and compared. Thus, this study aimed to compare the total phenolic contents, total flavonoid contents, total antioxidant capacities, and anti-tyrosine activity of eleven Sargassum species harvested off the Korean coast. The results revealed that the total phenolic content (from 20.57 to 88.97 mg gallic acid equivalent/g dry weight (dw)), flavonoid content (from 22.08 to 82.33 mg quercetin equivalent/g dw), anti-tyrosinase activity (from 13.30 to 126.30 mg kojic acid equivalent/dw), and antioxidant capacities of the 11 Sargassum species had wide ranges. Among them, S. miyabei Yendo and S. hemiphyllum showed the highest total antioxidant capacities while S. miyabei Yendo exhibiting the highest total phenolic and flavonoid contents. The highest anti-tyrosinase activity was seen in S. fillicinum and S. yendoi. Sargahydroqunoic acid and sargachromanol, two alga-derived meroterpenoid compounds with strong antioxidant activity, were detected and quantified in S. miyabei Yendo and S. serratifolium. Our findings guarantee further investigation of the health benefits of Sargassum species and maximize the commercial usage of these species.

scholarly journals Phenolic content and antioxidant activity of fruit of Brazilian genotypes of feijoa

2017 ◽  
Vol 52 (12) ◽  
pp. 1223-1230 ◽  
Author(s):  
Cassandro Vidal Talamini do Amarante ◽  
Alexandra Goede de Souza ◽  
Thalita Dal Toé Benincá ◽  
Cristiano André Steffens
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Fresh Weight ◽  
Functional Value ◽  
Dpph Method ◽  
Total Antioxidant ◽  
Acca Sellowiana ◽  
Gallic Acid Equivalent

Abstract: The objective of this work was to quantify the total phenolic content (TPC) and total antioxidant activity (TAA) of fruit from Brazilian genotypes of feijoa (Acca sellowiana). Skin and flesh tissues of the fruit were evaluated for the Alcântara, Helena, Mattos, and Nonante cultivars, and for the 2316 accession. TPC and TAA were both assessed in aqueous and hydroalcoholic extracts, and TAA was assayed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] methods. The TPC values, for aqueous extracts of the skin and flesh tissues, were 114.9 and 88.3 mg gallic acid equivalent (GAE) per 100 g fresh weight (FW), respectively. The corresponding values for TAA determined by the DPPH method, the skin and flesh tissues, given as EC50 (substrate concentration which leads to 50% reduction of the DPPH), were 10.7 and 39.7 mg FW per g DPPH, respectively; and, by the ABTS method, the TAA values were 12.01 and 8.33 μmol L-1 Trolox per g FW, respectively. The greatest values for TPC and TAA were obtained for 'Mattos' and 'Nonante', in the skin, and for 'Nonante' and accession 2316, in the flesh. Feijoa fruit has important functional value, particularly in the skin, characterized by high TPC and TAA.

scholarly journals Qualitative and Quantitative Phytochemical Screening of Bitter and Neem Leaves and their Potential as Antimicrobial Growth Promoter in Poultry Feed

2021 ◽  
pp. 38-49
Author(s):  
Ruth T. S. Ofongo ◽  
Elijah I. Ohimain ◽  
Eustace A. Iyayi
Keyword(s):  
Quantitative Analysis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Qualitative And Quantitative ◽  
Gastrointestinal Health ◽  
Neem Leaves ◽  
Gallic Acid Equivalent

Gastrointestinal health challenges without in-feed antibiotics are a crucial concern to poultry farmers. Although, quite a few substitutes for antibiotics have emerged, phytogenics and antibiotic properties of medicinal plants cannot be overlooked with regard to control, good health and minimizing sub – clinical bacteria-induced infections by zoonotic enteropathogenic bacteria in poultry birds. Vernonia amygdalina (Bitter leaf) and Azadirachta indica (Neem) leaves were collected early in the morning and oven dried to 15% moisture content. The non-essential oil was obtained by cold maceration method using methanol. Appropriate methods were used for the qualitative and quantitative analysis of phytochemicals in the respective oils. Qualitative analysis of phytochemicals showed that, saponins and terpenoids were highly detected (+++) in V. amygdalina. Saponins and terpenoids were slightly detected (+) in A. indica. Phenolic compounds were highly detected (+++) in both plants. Flavonoids were also identified (++) in both plants, but tannins were highly detected (+++) in A. indica. Quantitative analysis revealed a rather high concentration of complete phenolic content and overall flavonoid content in V. amygdalina. Total phenolic content of 44.76 mg Gallic acid equivalent / g of extract and 57.21 mg Rutin equivalent / g of extract (total flavonoid content) was recorded in V. amygdalina and 25.77 mg Gallic acid equivalent / g of extract (total phenolic content) and 24.45 mg Rutin equivalent /g of extract (total flavonoid content) in A. indica. In conclusion, both plant extracts exhibited possibility as suitable substitutes to proprietary antibiotics that can be beneficial to gastrointestinal health and overall performance in animal nutrition.

scholarly journals Phytochemical Composition and in vitro Antioxidant Activity of Golden Melon (Cucumis melo L.) Seeds for Functional Food Application

2019 ◽  
pp. 1-13 ◽  
Author(s):  
Ibidapo P. Olubunmi ◽  
A. Alagbe Olajumoke ◽  
J. Awogbemi Bamidele ◽  
O. Fatunmibi Omolara
Keyword(s):  
Cucumis Melo ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Seed Extracts ◽  
In Vitro Antioxidant Activity

Aim: Golden melon (Cucumis melo) is an annual herbaceous plant belonging to the family of Cucurbitaceae (Cucurbit). This study was carried out to evaluate the phytochemical composition and in vitro antioxidant activity of golden melon seed extract. Place and Duration of Study: The study was carried out between a period of July and August 2017 at Baking Milling Division, Federal Institute of Industrial Research Oshodi Nigeria.  Methodology: The crude methanolic extracts of the seed were tested for phytochemical and antioxidant activities according to standard analytical procedure. The antioxidant potential of the seed extracts was examined using different assays by determining total phenolic content, total flavonoid content, total antioxidant capacity. The free radical scavenging activities of the extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)  radical scavenging activity,  in vitro lipid peroxidation, and nitric oxide (NO) scavenging assay were determined spectrophotometrically. Results: The phytochemical screening of the seed extracts revealed the presence of some secondary metabolites such as alkaloids, phenolic, steroids, flavonoids, terpenoids and cardiac glycosides. The total phenolic content of extract was found to be 29.39 mg/100 g while the amount of total flavonoid content was 20.67 mg/100 g. Scavenging ability was observed to increase in proportion to concentration for all the scavenging assays and at the highest concentration of              100 µg/ml. Total antioxidant capacity assay showed 19.44 mg per 100 g. This high scavenging ability in the seed extracts may be attributed to the presence of phenolic and flavonoids compounds in the extract. The DPPH free radical scavenging activity of 100µg/ml Cucumis melo extract was 75.20% ± 0.72 while the reference standard (Ascorbic acid) was 83.24% ±0.31. Lipid peroxidation inhibition ability of 100 µg/ml Cucumis melo extract was 87.18% ± 0.16 while the standard (ascorbic acid) was 94.96% ± 0.16 at the same concentration. Results obtained from this study showed that the nitric oxide scavenging ability of the extract was 80.50%±0.63 while the standard antioxidant was 85.94% ± 0.54. Conclusion: In all the assays, Cucumis melo extract showed maximum percentage of antioxidant potentials at 100 µg/ml. Additionally, golden melon seed possess appreciable amount of phenols and high antioxidant properties which could be explored and incorporated in functional food applications particularly in baked products. 

scholarly journals In vitro antioxidant, anti-inflammatory and thrombolytic properties of leaf extract and fractions of Pterocarpus mildbraedii harms

2021 ◽  
Vol 22 (3) ◽  
pp. 65-74
Author(s):  
O.A. Fajobi ◽  
B.O. Emma-Okon ◽  
O.O. Oyedapo
Keyword(s):  
Methanolic Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Beneficial Effects ◽  
Total Antioxidant ◽  
Anti Inflammatory ◽  
Ig G ◽  
Gallic Acid Equivalent

The study was undertaken to evaluate the in vitro antioxidant, anti-inflammatory and thrombolytic properties of methanolic extract (PMME) and fractions of the leaf of Pterocarpus mildbraedii with a view to exploring the therapeutic potentials of the plant especially as far as treatment and management of oxidative, inflammatory and heart related disorders are concerned. The study involved collection, identification, extraction with 80%methanol, fractionation of hydromethanolic extract of P. mildbraedii leaves with n-hexane, ethyl acetate and nbutanol; evaluation of the contents for total phenolics, flavonoids as well as vitamins C and E. Evaluation of the antioxidant and anti-inflammatory potentials of the plant using 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power, hydrogen peroxide reduction, total antioxidant capacity (TAC), and stabilization of erythrocyte membrane and inhibition of albumin denaturation were also carried out. The total phenolic content ranged between 13.31 ± 0.12 and 234.99 ± 2.64 GAE (gallic acid equivalent)/g, flavonoid content ranged between 44.30 ± 0.74 and 228.74 ± 7.44 QE (quercetin equivalent)/g, vitamin C content was 11.98 ± 0.04 mg/g while vitamin E content was 8.62 ± 0.29 ìg/g. The fractions exhibited potent and significant antioxidant and antiinflammatory activities which are concentration-dependent, as well as appreciable thrombolytic activities. Moreover, the extract and fractions compared favourably with various reference drugs used in the study. It was observed that the extract and its fractions possessed bioactive components with beneficial effects in the management of oxidative, inflammatory and heart-related conditions. Keywords: Pterocarpus mildbraedii methanolic extract, Antioxidant, Anti-inflammatory, Thrombolytic, Membrane stability, Haemoglobin, Denaturation.

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