scholarly journals Gibberellic Acid Production by Different Fermentation Systems Using Citric Pulp as Substrate/Support

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Juliana de Oliveira ◽  
Cristine Rodrigues ◽  
Luciana P. S. Vandenberghe ◽  
Marcela C. Câmara ◽  
Nelson Libardi ◽  
...  

Gibberellic acid (GA3) is an important phytohormone, a member of gibberellins family, which acts as a promoter and regulator of plant growth. This study aimed to evaluate GA3 production by Fusarium moniliforme LPB03 and Gibberella fujikuroi LPB06 using different techniques of fermentation, solid state fermentation (SSF), submerged fermentation (SmF), and semisolid state fermentation (SSSF), and different types of bioreactors. In all techniques, citric pulp (CP), a subproduct obtained from the extraction of orange juice, was employed as the substrate/support. GA3 production by SSF reached 7.60 g kg−1 and 7.34 g kg−1 in Erlenmeyer flasks and column bioreactors, respectively. For SmF, the highest concentration of GA3 obtained was 236.00 mg L−1 in Erlenmeyer flasks, 273.00 mg L−1 in a 10 L stirred tank reactor (STR), and 203.00 mg L−1 in a 1.5 L bubble column reactor (BCR). SSSF was conducted with a CP suspension. In this case, GA3 concentration reached 331.00 mg L−1 in Erlenmeyer flasks and 208 mg L−1 in a BCR. The choice of the fermentation technique is undoubtedly linked to the characteristics and productivity of each process. The methods studied are inexpensive and were found to produce good proportions of GA3, making them suitable for several applications.

2014 ◽  
Vol 592-594 ◽  
pp. 2427-2431 ◽  
Author(s):  
Sushovan Chatterjee ◽  
Sanjoy Paul

Under optimum environmental conditions, microalgae are able to accumulate significant amounts of lipids within diminutive span. They are therefore established as promising candidates for the production of biodiesel. In order to develop this, and also to cut down the cost parameter involving the production of biodiesel from algae, the growth rate of algae has to be enhanced. To maintain the proper condition for their growth, closed cultivation is the best option for which different types of reactor are used. Bubble column reactor, is one among them, where the mixing rate of algae with nutrients, growth rate increases. This paper reported the design modification of spiral column reactor which will have better mixing rate than conventional bubble column reactor in turn more mass transfer due to more turbulence which can be seen by analyzing the turbulence kinetic energy against radius for both the reactors, for that CFX solver is been used.


Energies ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 4130 ◽  
Author(s):  
Okkyoung Choi ◽  
MinJeong Kim ◽  
Youngwook Go ◽  
Moon-Gi Hong ◽  
Bomin Kim ◽  
...  

Methane production was carried out in two different types of reactors using a thermophilic and hydrogenotrophic methanogen, Methanothermobacter sp. KEPCO-1, which converts hydrogen and carbon dioxide into methane at 60 °C. The two reactors used for methane production were stirred-tank reactor (ST) and a bubble column reactor (BC), which were selected because they can provide a good comparison between the medium agitation type and gas–liquid mass transfer. The specific growth rate of KEPCO-1 in the ST and BC was 0.03 h−1 and 0.07 h−1, respectively. The methane conversion rate increased to 77.8 L/L/d in the ST and 19.8 L/L/d in the BC. To prevent the dilution of nutrients in the medium by the water generated during the hydrogenotrophic methanation reaction, a membrane distillation (MD) process was applied to selectively remove water from the culture medium. The MD process selectively removed only water from the medium. Fouling by KEPCO-1 had a negligible effect on flux and showed a high removal performance flux of 16.3 ± 3.1 L/m2/h. By operating the MD process in conjunction with the hydrogenotrophic methanation process, it is possible to prevent the dilution of the nutrients in the medium by the water generated during the methanation process, thereby maintaining stable microbial growth and methanation activity.


Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 626
Author(s):  
Alexander Rudolph ◽  
Amna El-Mohamad ◽  
Christopher McHardy ◽  
Cornelia Rauh

Fruits have an important economic impact in the context of plant-based food production. The consumption of fruit juices, mostly produced from concentrates, is particularly noteworthy. Conventional concentration methods do not always enable a sustainable and gentle concentration. The innovative gas hydrate technology addresses this point with its energy-saving, gentle character, and high concentration potential. In this study, the concentration of fruit juices and model solutions using CO2 hydrate technology was investigated. To find a suitable operating point for hydrate formation in the used bubble column, the hydrate formation in a water–sucrose model solution was evaluated at different pressure and temperature combinations (1, 3, 5 °C and 32.5, 37.5, 40 bar). The degrees of concentration indicate that the bubble column reactor operates best at 37.5 bar and 3 °C. To investigate the gentle processing character of the hydrate technology, its quantitative effects on vitamin C, betanin, polyphenols, and carotenoids were analyzed in the produced concentrates and hydrates via HPLC and UV/VIS spectrophotometry. The results for fruit juices and model solutions imply that all examined substances are accumulated in the concentrate, while only small amounts remain in the hydrate. These amounts can be related to an inefficient separation process.


Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 175-189 ◽  
Author(s):  
Jin-rong Xu ◽  
John F Leslie

Abstract We constructed a recombination-based map of the fungal plant pathogen Gibberella fujikuroi mating population A (asexual stage Fusarium moniliforme). The map is based on the segregation of 142 restriction fragment length polymorphism (RFLP) markers, two auxotrophic genes (arg1, nic1), mating type (matA+ / matA−), female sterility (ste1), spore-killer (Sk), and a gene governing the production of the mycotoxin fumonisin B1 (fum1) among 121 random ascospore progeny from a single cross. We identified 12 linkage groups corresponding to the 12 chromosome-sized DNAs previously observed in contour-clamped homogeneous electric field (CHEF) gels. Linkage groups and chromosomes were correlated via Southern blots between appropriate RFLP markers and the CHEF gels. Eleven of the 12 chromosomes are meiotically stable, but the 12th (and smallest) is subject to deletions in 3% (4/121) of the progeny. Positive chiasma interference occurred on five of the 12 chromosomes, and nine of the 12 chromosomes averaged more than one crossover per chromosome. The average kb/cM ratio in this cross is ~32.


2017 ◽  
Author(s):  
Nur Afizah Ibrahim ◽  
Amir Khalid ◽  
Izzuddin Zaman ◽  
Azwan Sapit ◽  
Bukhari Manshoor

Fuel ◽  
2021 ◽  
pp. 122481
Author(s):  
Hung Hai Pham ◽  
Suk Hyun Lim ◽  
Kang Seok Go ◽  
Nam Sun Nho ◽  
Eun Hee Kwon ◽  
...  

2009 ◽  
Vol 146 (2) ◽  
pp. 184-188 ◽  
Author(s):  
José M. Navaza ◽  
Diego Gómez-Díaz ◽  
M Dolores La Rubia

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