scholarly journals Rapid Screening of Chemical Constituents in Rhizoma Anemarrhenae by UPLC-Q-TOF/MS Combined with Data Postprocessing Techniques

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Lanlan Shan ◽  
Yuanyuan Wu ◽  
Lei Yuan ◽  
Yani Zhang ◽  
Yanyan Xu ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Neutral Loss ◽  
Rapid Screening ◽  
Steroidal Saponins ◽  
Chemical Research ◽  
Literature Reviews ◽  
Different Types ◽  
Fragmentation Patterns ◽  
Anemarrhena Asphodeloides ◽  
Tof Ms

Rhizoma Anemarrhenae, a famous traditional Chinese medicine (TCM), is the dried rhizome of Anemarrhena asphodeloides Bge. (Anemarrhena Bunge of Liliaceae). The medicine presents anti-inflammatory, antipyretic, sedative, and diuretic effects. The chemical constituents of Rhizoma Anemarrhenae are complex and diverse, mainly including steroidal saponins, flavonoids, phenylpropanoids, benzophenones, and alkaloids. In this study, UPLC-Q-TOF/MS was used in combination with data postprocessing techniques, including characteristic fragments filter and neutral loss filter, to rapidly classify and identify the five types of substances in Rhizoma Anemarrhenae. On the basis of numerous literature reviews and according to the corresponding characteristic fragments produced by different types of compounds in combination with neutral loss filtering, we summarized the fragmentation patterns of the main five types of compounds and successfully screened and identified 32 chemical constituents in Rhizoma Anemarrhenae. The components included 18 steroidal saponins, 6 flavonoids, 4 phenylpropanoids, 2 alkaloids, and 2 benzophenones. The method established in this study provided necessary data for the study on the pharmacological effects of Rhizoma Anemarrhenae and also provided the basis for the chemical analysis and quality control of TCMs to promote the development of a method for chemical research on TCMs.

scholarly journals Rapid Screening and Quantitative Determination of Active Components in Qing-Hua-Yu-Re-Formula Using UHPLC-Q-TOF/MS and HPLC-UV

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Xin Shao ◽  
Jie Zhao ◽  
Xu Wang ◽  
Yi Tao
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Chemical Constituents ◽  
Rapid Screening ◽  
Active Components ◽  
Limit Of Quantification ◽  
Unknown Compound ◽  
Salvianolic Acid ◽  
Tof Ms

Qing-Hua-Yu-Re-Formula (QHYRF), a new herbal preparation, has been extensively used for treating diabetic cardiomyopathy. However, the chemical constituents of QHYRF remain uninvestigated. In the present study, rapid ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to qualitatively analyze the components of QHYRF. Qualitative detection was performed on a Kromasil C18 column through the gradient elution mode, using acetonitrile-water containing 0.1% formic acid. Twenty-seven compounds were identified or tentatively characterized, including 12 phenolic acids, nine monoterpene glycosides, two flavonoids, three iridoids, and one unknown compound. Among these, six compounds were confirmed by comparing with standards. A high-performance liquid chromatography (HPLC) method was developed to simultaneously determine the following six active components in QHYRF: danshensu, paeoniflorin, acteoside, lithospermic acid, salvianolic acid B, and salvianolic acid C. These HPLC chromatograms were monitored at 254, 280, and 320 nm. The method was well validated with respect to specificity, linearity, limit of detection, limit of quantification, precision, stability, and recovery. The HPLC-UV method was successfully applied to 10 batches of QHYRF.

scholarly journals Determination of Alkaloids and Flavonoids in Sophora flavescens by UHPLC-Q-TOF/MS

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yaqian Dong ◽  
Guoxiang Jia ◽  
Jingwen Hu ◽  
Hui Liu ◽  
Tingting Wu ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Neutral Loss ◽  
Relevant Literature ◽  
Sophora Flavescens ◽  
Fragment Ions ◽  
Mobile Phases ◽  
Characteristic Fragment ◽  
Fragmentation Rules ◽  
Tof Ms

This study is based on UHPLC-Q-TOF/MS and fragment ions to achieve classification and identification of alkaloids and flavonoids in Sophora flavescens. By reviewing the available and relevant literature, the mass fragmentation rules of alkaloids and flavonoids were summarized. 0.1% formic acid water (A) and acetonitrile (B) were used as mobile phases. 37 chemical constituents were identified, including 13 alkaloids and 24 flavonoids. This research method offers a complete strategy based on the fragmentation information of characteristic fragment ions and neutral loss obtained by MS/MS to characterize the chemical composition of Sophora flavescens.

scholarly journals Rapid Identification of the Chemical Components of Ilex rotunda Thunb Using UPLC-Q-TOF-MS/MS

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Xinxin Chen ◽  
Junmao Li ◽  
Renyikun Yuan ◽  
Youqiong Zhuo ◽  
Yangling Chen ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Neutral Loss ◽  
Sample Pretreatment ◽  
Practical Method ◽  
Chemical Components ◽  
Flavonoid Glycosides ◽  
Active Components ◽  
Triterpenoid Saponins ◽  
Ilex Rotunda ◽  
Tof Ms

Ilicis Rotundae Cortex (IRC) consists of the bark of Ilex rotunda Thunb, and its chemical constituents mainly include flavonoid glycosides, phenols, and triterpenoid saponins. In this study, a preliminary analysis was performed to identify and obtain the chemical components from IRC to better control the quality of the medicinal materials and provide a chemical basis for the study of the efficacy of the active components. Simple and efficient sample pretreatment and ultrasonic-assisted extraction methods were used to analyze the mass spectrum fragments and fracture modes in the anion mode by UPLC-Q-TOF-MS/MS. Using a two-step strategy, the neutral loss, diagnostic ions, and characteristic fragments were studied to screen diverse skeletons and substitutions, and the possible compounds were identified by comparison with databases. The representative compounds were compared with the standard, and the mass spectrogram was found to match perfectly. Thus, our findings reveal that this method is feasible and reliable and can be used to analyze the chemical components of IRC. We identified 105 compounds, including 22 triterpenoid saponins, 15 chlorogenic acids, 33 phenylpropanoids and phenylpropanosides, 3 iridoids, 1 flavonoid, 10 lignans, 12 glycosides, and 9 other compounds. This method lays the foundation for further elucidating the pharmacodynamics of IRC and provides a practical method for the identification of IRC.

HPLC-TOF/MS in identification of chemical constituents of Fuzhengpingxiao Capsule

2013 ◽  
Vol 32 (7) ◽  
pp. 770-779
Author(s):  
Liang ZHAO ◽  
Wen-jun TIAN ◽  
Lei LU ◽  
Hai ZHANG ◽  
Xin-xia WANG ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

Qualitative characterization of chemical constituents in Diwu-Yanggan-Fang by UPLC-Q-TOF/MS

2014 ◽  
Vol 6 (6) ◽  
pp. 725
Author(s):  
Chao Zhou ◽  
Hong-mei Jia ◽  
Hong-wu Zhang ◽  
Zhong-mei Zou
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

scholarly journals New Steroidal Saponins from the Rhizomes of Anemarrhena asphodeloides BUNGE (Liliaceae).

1994 ◽  
Vol 42 (11) ◽  
pp. 2342-2345 ◽  
Author(s):  
Setsuo SAITO ◽  
Satoshi NAGASE ◽  
Koki ICHINOSE
Keyword(s):  
Steroidal Saponins ◽  
Anemarrhena Asphodeloides

scholarly journals Quantification of Fragments of Human Serum Inter-α-Trypsin Inhibitor Heavy Chain 4 by a Surface-Enhanced Laser Desorption/Ionization-Based Immunoassay

2006 ◽  
Vol 52 (6) ◽  
pp. 1045-1053 ◽  
Author(s):  
Jin Song ◽  
Manisha Patel ◽  
C Nicole Rosenzweig ◽  
Yee Chan-Li ◽  
Lori J Sokoll ◽  
...  
Keyword(s):  
Human Serum ◽  
Heavy Chain ◽  
Trypsin Inhibitor ◽  
Laser Desorption ◽  
Blood Collection ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Surface Enhanced ◽  
Fragmentation Patterns ◽  
Tof Ms

Abstract Background: Several proteolytically derived fragments from the proline-rich region (PRR) of human inter-α-trypsin inhibitor heavy chain 4 (ITIH4) have been identified by surface-enhanced or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS or MALDI-TOF-MS) as potential disease markers. Methods: Previously, we developed a SELDI-based immunoassay that can simultaneously distinguish and quantify multiple isoforms/variants of a protein/peptide of interest. In this study, we used this high-throughput approach to quantify and characterize the extensive fragmentation within the PRR of human serum ITIH4 and determined its association with different disease conditions. The ITIH4-related fragments were first immunocaptured by use of beads coupled with peptide-specific antibodies. The eluates were then studied by SELDI-TOF-MS. In addition, freshly collected and immediately processed serum and plasma samples were used to analyze the ex vivo stability of these ITIH4 fragments. Results: Human serum ITIH4 was shown to be extensively proteolytically processed within the PRR, and its fragmentation patterns were closely associated with different disease conditions. Fragmentation patterns were generally consistent with cleavages by endoprotease followed by exoprotease actions. Observed fragments changed little under different assay conditions or blood collection and processing procedures. Conclusions: The fragmentation patterns within the PRR of human serum ITIH4 are associated with different disease conditions and may hold important diagnostic information. These fragmentation patterns could be useful as potential biomarkers for detection and classification of cancer.

scholarly journals What you need to know about scoping reviews

Medwave ◽  
2021 ◽  
Vol 21 (02) ◽  
pp. e8144-e8144
Author(s):  
Catalina Verdejo ◽  
Luis Tapia-Benavente ◽  
Bastián Schuller-Martínez ◽  
Laura Vergara-Merino ◽  
Manuel Vargas-Peirano ◽  
...  
Keyword(s):  
Systematic Reviews ◽  
Scoping Review ◽  
Clinical Epidemiology ◽  
Recent Model ◽  
Reporting Guideline ◽  
Scoping Reviews ◽  
Literature Reviews ◽  
Different Types ◽  
Reproducible Method ◽  
Meta Analyses

The increasing amount of evidence has caused an increasing amount of literature reviews. There are different types of reviews —systematic reviews are the best known—, and every type of review has different purposes. The scoping review is a recent model that aims to answer broad questions and identify and expose the available evidence for a broader question, using a rigorous and reproducible method. In the last two decades, researchers have discussed the most appropriate method to carry out scoping reviews, and recently the “Preferred Reporting Items for Systematic Reviews and Meta-Analyses’ for scoping reviews (PRISMA-ScR) reporting guideline was published. This is the fifth article of a methodological collaborative series of narrative reviews about general topics on biostatistics and clinical epidemiology. This review aims to describe what scoping reviews are, identify their objectives, differentiate them from other types of reviews, and provide considerations on how to carry them out.

scholarly journals Characterization and identification of flavonoids from Bambusa chungii leaves extract by UPLC-ESI-Q-TOF-MS/MS

2020 ◽  
Author(s):  
Ting Yuan ◽  
Xue-Feng Guo ◽  
Si-Yue Shao ◽  
Rong-Miao An ◽  
Jin Wang ◽  
...  
Keyword(s):  
High Performance ◽  
Gradient Elution ◽  
High Definition ◽  
Bamboo Leaves ◽  
Fat Reduction ◽  
Accurate Mass Measurements ◽  
Acid Aqueous Solution ◽  
Characteristic Fragmentation ◽  
Fragmentation Patterns ◽  
Tof Ms

AbstractBamboo leaves extract (BLE) has a variety of physiological functions such as antitumour, anti-inflammatory, antioxidant and blood fat reduction activities and the flavonoids of bamboo leaves are the major active constituents. To profile the flavonoids in the complex BLE, a rapid and sensitive analytical method based on ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) was developed for the structural identification of the flavonoids in Bambusa chungii leaves extract using accurate mass measurements and characteristic fragmentation patterns. After separation on an Agilent SB-C18 Rapid Resolution High Definition (RRHD) column (2.1 mm × 150 mm, 1.8 μm) by gradient elution with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase, the sample was analysed by ESI-QTOF-MS/MS in the negative mode. A total of 22 flavonoids were detected, and eight of these were identified by comparison with reference standards, while the other fourteen were tentatively identified according to their MS/MS data. The main fragmentation pathways of flavonoid C-glycosides (compounds 1, 5 and 10), flavonoid di-C,O-glycosides (compound 4), flavonoid di-C-glycosides (compound 7) and flavonoid C,O-di-glycosides (compounds 2 and 14) are shown in this article. This is the first report on the analysis of the flavonoids in the extract of B. chungii leaves. The present work demonstrates that UPLC-ESI-Q-TOF-MS/MS is an efficient technique for identifying multiple flavonoids of BLE.

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