scholarly journals The Distribution Frequency of Interferon-Gamma Receptor 1 Gene Polymorphisms in Interferon-γ Release Assay-Positive Patients

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
Changguo Chen ◽  
Lei Chen ◽  
Changwei Chen ◽  
Qiuyuan Chen ◽  
Qiangyuan Zhao ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Promoter Region ◽  
Gene Polymorphisms ◽  
Statistical Significance ◽  
Interferon Γ ◽  
Mutation Rates ◽  
Gamma Receptor ◽  
Release Assay ◽  
Positive Groups ◽  
Distribution Frequency

Tuberculosis is caused by mycobacterium, a potentially fatal infectious bacterium. In recent years, TB cases increased in the whole world. WHO statistics data shows that the world’s annual tuberculosis incidence was 8~10 million with about 3 million deaths. Several studies have shown that susceptibility to tuberculosis may be associated with IFNGR1 gene polymorphisms. Here, we report the distribution frequency of IFNGR1 gene polymorphisms in 103 cases of IGA-negative patients and 100 cases of IGA-positive patients from China by sequencing the IFNGR1 proximal ~750 bp promoter region. We found a total of 5 types of site mutations: -611 (G/A), -56 (T/C), -255 (C/T), -359 (T/C), and -72 (C/T). The two main types of gene polymorphisms among the IGA-negative and IGA-positive groups were -611 (G/A), with mutation rates of 88.3% and 78.4%, respectively, and -56 (T/C), with mutation rates of 84.5% and 83.8%, respectively, which had no statistical significance, and there was no correlation with the incidence of tuberculosis.

scholarly journals 1344. Interferon Gamma Release Assay (IGRA) Responses in HIV-Infected and -Uninfected Women in Pregnancy

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S486-S487
Author(s):  
Samantha Kaplan ◽  
Daniel Matemo ◽  
Jerphason O Mecha ◽  
John Kinuthia ◽  
Grace John-Stewart ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Test Performance ◽  
Statistical Significance ◽  
Preventive Therapy ◽  
Categorical Variables ◽  
Interferon Gamma Release Assay ◽  
Hiv Positive Women ◽  
Release Assay ◽  
Hiv Women ◽  
Latent Tb Infection

Abstract Background Pregnancy and HIV-associated immunologic changes may affect latent TB infection (LTBI) interferon-gamma release assay (IGRA) QuantiFERON TB Gold Plus (QFT-Plus) diagnostic performance. Methods In this ongoing study, HIV-infected and -uninfected women 20–34 weeks gestation without TB in the past year are enrolled from antenatal clinics in western Kenya and tested with QFT-Plus. Mean quantitative IFN- γ responses to mitogen, and M. tuberculosis antigens (TB1 [primarily CD4+] and TB2 [addition of CD8+ response]) were compared using two-sample t-tests. Proportions for categorical variables were compared using univariate logistic regression. Results Among 306 women (HIV+ 127 [41.5%], HIV− 179 [58.5%]) enrolled between January 2018 and March 2019, median maternal and gestational age were 25 years (IQR 21–28) and 28 weeks (IQR 24–32), respectively. Among HIV-infected women at enrollment, 99.2% were on ART, median CD4 count was 440 cells/mm3 (IQR 235–703), 37.5% were virally suppressed, and 60.6% reported having received isoniazid preventive therapy (IPT). Overall, 95 (31.1%) women were QFT-Plus positive (HIV+ 38 [29.9%], HIV− 57 [31.8%], OR 0.90, 95% CI 0.54–1.48, P = 0.671); 190 (62.1%) were negative (HIV+ 81 [63.8%], HIV− 109 [60.9%]), and 21 had indeterminate results (HIV+ 8 [6.3%], HIV− 13 [7.3%], OR 0.83, 95% CI 0.33–2.09, P = 0.690). Mean response to mitogen was similar between HIV-infected and -uninfected women (6.0 vs. 6.1 IU/mL, P = 0.663]. Among QFT-Plus positive women, HIV+ women had significantly lower TB1 responses than HIV− women (HIV+ 2.7 vs. 4.2 IU/mL, P = 0.035). Mean TB2 responses had a similar pattern, but did not reach statistical significance (HIV+ 3.1 vs. 4.3 IU/mL, P = 0.107). Both TB1 and TB2 were positive for 82 women (86.3%), 4 women were only TB1 positive (4.2%), and 8 women were only TB2 positive (8.4%). Conclusion Among pregnant women, HIV-infection was not associated with increased prevalence of QFT+ responses. However, among QFT-positive women, TB1 responses were lower in HIV-positive women with a similar trend observed for TB2 responses. These findings suggest that HIV-associated immunologic changes may influence QFT test performance. Disclosures All authors: No reported disclosures.

scholarly journals 1390. A Novel Application of the Interferon-Gamma Release Assay (IGRA) Among End-Stage Heart Failure Patients Awaiting Heart Transplantation

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S505-S505
Author(s):  
Ming-Jui Tsai ◽  
Aristine Cheng ◽  
Hsin-Yun Sun ◽  
Yih-Sharng Chen ◽  
Nai-Kuan Chou ◽  
...  
Keyword(s):  
Heart Failure ◽  
Heart Transplantation ◽  
Interferon Gamma ◽  
Heart Transplant ◽  
Interferon Γ ◽  
Positive Control ◽  
Cell Mediated Immunity ◽  
Release Assay ◽  
Transplant Candidates ◽  
Overall Mortality

Abstract Background The optimal approach to assay the immune status in heart failure is challenging because of the inherent complexity of chronic inflammation. Interferon-gamma release assays (IGRAs) measure an aspect of cell-mediated immunity encompassing both the innate and adaptive immunity. In this study, we evaluated the utility of a commercial IGRA for predicting mortality and infectious complications among heart transplant candidates. Methods This prospective cohort study was conducted between August 1, 2014 and January 31, 2019 at a medical center in Taiwan. All heart transplant candidates received an IGRA (QuantiFERON®-TB Gold In-Tube, QFT-GIT) at baseline as part of the initiative to screen for latent tuberculosis. Impaired cell-mediated immunity was defined as the release of <1 IU/mL of interferon-γ (IFN-γ) in response to the common mitogen in the positive control tube.The patients were then followed until death or January 31, 2019. Results A total of 102 patients were enrolled; of whom, 23 (22.5%) had impaired cell-mediated immunity at baseline. During the study period with a median follow-up of 1.90 years (IQR 1.17–3.56), 23 (22.5%) patients died and 45 (44.1%) patients developed an infectious complication. Overall mortality was significantly greater among those with impaired cell-mediated immunity [39.1% (9/23) vs. 17.7% (14/79), P = 0.031]. A trend toward higher rates of infection was observed among impaired cell-mediated immunity group [60.9% (14/23) vs. 39.2% (31/79), P = 0.066]. The most common cause of death was infection (56.5%). No patient developed active tuberculosis during the study and the most common infection was bacteremia (35.6%). In the age-adjusted multivariate analysis, impaired cell-mediated immunity was an independent predictor of mortality (HR 2.87, CI 1.23–6.68, P = 0.014) and subsequent infectious event (HR 3.00, CI 1.56–5.76, P = 0.001). Conclusion An interferon-γ release assay utilizing the positive control tube of the QuantiFERON®-TB Gold In-Tube kit was predictive of overall mortality and infections among patients with advanced heart failure awaiting heart transplantation. Disclosures All authors: No reported disclosures.

scholarly journals Influence of interferon-gamma Receptor 1 gene polymorphisms on the susceptibility to pulmonary tuberculosis among sudanese population

2018 ◽  
Vol 7 (1) ◽  
pp. 26
Author(s):  
MogahidMohammed Elhassan ◽  
AfraHasabelrsoul Ali ◽  
AlfadhilAlobeid Omer ◽  
NageebSuleiman Saeed ◽  
ElhagE Mansour
Keyword(s):  
Pulmonary Tuberculosis ◽  
Interferon Gamma ◽  
Gene Polymorphisms ◽  
Gamma Receptor
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