scholarly journals One-Pot Aqueous Synthesis of Fluorescent Ag-In-Zn-S Quantum Dot/Polymer Bioconjugates for Multiplex Optical Bioimaging of Glioblastoma Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Alexandra A. P. Mansur ◽  
Herman S. Mansur ◽  
Sandhra M. Carvalho ◽  
Anderson J. Caires
Keyword(s):  
Optical Properties ◽  
Room Temperature ◽  
Causes Of Death ◽  
Glioma Cells ◽  
Human Embryonic Kidney Cell ◽  
One Pot ◽  
Aqueous Synthesis ◽  
Water Dispersible ◽  
Malignant Glioma Cells

Cancer research has experienced astonishing advances recently, but cancer remains a major threat because it is one of the leading causes of death worldwide. Glioblastoma (GBM) is the most malignant brain tumor, where the early diagnosis is vital for longer survival. Thus, this study reports the synthesis of novel water-dispersible ternary AgInS2 (AIS) and quaternary AgInS2-ZnS (ZAIS) fluorescent quantum dots using carboxymethylcellulose (CMC) as ligand for multiplexed bioimaging of malignant glioma cells (U-87 MG). Firstly, AgInS2 core was prepared using a one-pot aqueous synthesis stabilized by CMC at room temperature and physiological pH. Then, an outer layer of ZnS was grown and thermally annealed to improve their optical properties and split the emission range, leading to core-shell alloyed nanostructures. Their physicochemical and optical properties were characterized, demonstrating that luminescent monodispersed AIS and ZAIS QDs were produced with average sizes of 2.2 nm and 4.3 nm, respectively. Moreover, the results evidenced that they were cytocompatible using in vitro cell viability assays towards human embryonic kidney cell line (HEK 293T) and U-87 MG cells. These AIS and ZAIS successfully behaved as fluorescent nanoprobes (red and green, resp.) allowing multiplexed bioimaging and biolabeling of costained glioma cells using confocal microscopy.

Multivariate optimization of optical properties of CdSe quantum dots obtained by a facile one-pot aqueous synthesis

2019 ◽  
Vol 6 (6) ◽  
pp. 1350-1360 ◽  
Author(s):  
Isabelle Moraes Amorim Viegas ◽  
Beate Saegesser Santos ◽  
Adriana Fontes ◽  
Giovannia Araujo de Lima Pereira ◽  
Claudete Fernandes Pereira
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Cdse Quantum Dots ◽  
Multivariate Optimization ◽  
High Quality ◽  
One Pot ◽  
Aqueous Synthesis ◽  
Synthesis Procedure

Four parameters are simultaneously optimized to provide a simple, fast and reproducible synthesis procedure to obtain high-quality CdSe quantum dots.

One-Pot Microwave Synthesis of Water-Dispersible, High Fluorescence Silicon Nanoparticles and Their Imaging Applications in Vitro and in Vivo

2016 ◽  
Vol 88 (23) ◽  
pp. 11631-11638 ◽  
Author(s):  
Hong-Li Ye ◽  
Shi-Jiao Cai ◽  
Si Li ◽  
Xi-Wen He ◽  
Wen-You Li ◽  
...  
Keyword(s):  
Microwave Synthesis ◽  
Silicon Nanoparticles ◽  
One Pot ◽  
Water Dispersible ◽  
High Fluorescence

scholarly journals ATPS-42INHIBITION OF STAT3 ENHANCES THE RADIOSENSITIZING EFFECT OF TEMOZOLOMIDE IN MALIGNANT GLIOMA CELLS IN VITRO AND IN VIVO

2015 ◽  
Vol 17 (suppl 5) ◽  
pp. v27.3-v27
Author(s):  
Tae Jin Han ◽  
Eun Jung Choi ◽  
Bong Jun Cho ◽  
Sang Hyuk Song ◽  
Sun Ha Paik ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Glioma Cells ◽  
Radiosensitizing Effect ◽  
Malignant Glioma Cells

scholarly journals IL-10 Promotes Glioma Cell Growth and Invasion via Upregulation of KPNA2 In vitro

2019 ◽  
Author(s):  
Zihao Zhang ◽  
Xiaoming Huang ◽  
Jie Li ◽  
Haitao Fan ◽  
Fan Yang ◽  
...  
Keyword(s):  
Cell Growth ◽  
Glioma Cell ◽  
Causes Of Death ◽  
Glioma Cells ◽  
Primary Brain Tumor ◽  
Dual Function ◽  
Quantitative Real Time Pcr ◽  
High Incidence ◽  
Important Significance

Abstract Background Glioma is one of the leading causes of death worldwide with high incidence, recurrence and mortality. IL-10 is a cytokine with dual function in many types of tumors. Although IL-10 is overexpressed and promotes tumor progression in human primary brain tumor, the mechanisms are largely unknown. Methods Glioma cells were treated with different dosages of IL-10. The cell growth was detected by CCK8, and the invasion was measured by Transwell. The relative expression of mRNAs was detected by Quantitative real-time PCR (q-PCR). Results We found that IL-10 treatment significantly enhanced glioma cell growth and invasion. And KPNA2 was significantly upregulated after treatment with IL-10. By performing knockdown experiments, we found that the glioma cell growth and invasion were significantly declined. Conclusions The results indicated that knockdown of KPNA2 significantly inhibited the growth and invasion of glioma cells. And IL-10 promotes glioma progression via upregulation of KPNA2. This study will be of important significance and provides a potential target for treatment of patients with glioma.

scholarly journals One pot synthesis of silver nanoparticles using a cyclodextrin containing polymer as reductant and stabilizer

2014 ◽  
Vol 5 ◽  
pp. 380-385 ◽  
Author(s):  
Arkadius Maciollek ◽  
Helmut Ritter
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Silver Nanoparticles ◽  
Optical Properties ◽  
Silver Nitrate ◽  
Room Temperature ◽  
One Pot ◽  
One Pot Synthesis ◽  
Transmission Electron ◽  
Vis Spectroscopy

A facile and one pot synthesis of silver nanoparticles with narrow size distributions using silver nitrate and a copolymer 1 from N-isopropylacrylamide (NIPAM) and mono-(1H-triazolylmethyl)-2-methylacryl-β-cyclodextrin acting as reductant and stabilizer without using any additional reducing agent is reported. The reduction was carried out in aqueous solution under pH neutral conditions at room temperature. The results of dynamic light scattering analysis and transmission electron microscopy show adjustable particle sizes from 30–100 nm, due to variation of silver nitrate concentration, the polymeric reducing and stabilisation agent concentration or reaction time. The spherical structure of the silver nanoparticles has been confirmed by UV–vis spectroscopy and transmission electron microscopy. The optical properties of the nanoparticles have also been characterized by fluorescence spectroscopy. The formed spherical particles are stable in aqueous medium at room temperature over a period of several weeks. Furthermore the changes in the optical properties of the nanoparticles due to thermo induced volume phase transition behavior of the thermoresponsive cyclodextrin containing polymer 1 have been characterized by UV–vis spectroscopy.

Targeting adenovirus to CD80 and CD86 receptors increases gene transfer efficiency to malignant glioma cells

2007 ◽  
Vol 107 (3) ◽  
pp. 617-627 ◽  
Author(s):  
Ilya V. Ulasov ◽  
Angel A. Rivera ◽  
Yu Han ◽  
David T. Curiel ◽  
Zeng B. Zhu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Brain Tumors ◽  
Malignant Glioma ◽  
Malignant Gliomas ◽  
Glioma Cells ◽  
Luciferase Reporter ◽  
Transduction Efficiency ◽  
Wild Type ◽  
Malignant Glioma Cells

Object Gene therapy protocols for malignant gliomas utilize adenoviral vectors that rely almost exclusively on the adenovirus serotype 5 (Ad5) backbone. The authors have previously shown that chimeric vectors that bind to the Ad3 receptor, or CD46, increase the transduction efficiency of malignant brain tumors. In light of the debate regarding the efficacy of CD46 compared with CD80/CD86 in binding Ad3 virions, the authors now examine the expression and transduction efficiency of Ad5/3 chimeras that bind via CD80/CD86. Methods The authors first analyzed CD80/CD86 expression in glioma cell lines. They then used three replication-defective vectors containing a luciferase reporter gene: Ad5/3 (containing the tail and shaft domain of Ad5 and the knob domain of Ad3); Ad3/5 (containing the tail of Ad5, shaft of Ad3, and knob of Ad5); and Ad3/3 (containing the tail of Ad5, shaft of Ad3, and knob of Ad3). These vectors were analyzed both in vitro and in vivo against malignant glioma cells. To examine further the effect of Ad5/3 fiber modification, the authors created an oncolytic vector, conditionally replicative Ad5/3 (CRAd5/3). Results The Ad5/3 vector showed a 10- to 100-fold enhanced transduction efficiency of malignant glioma compared with replication-defective wild-type adenovirus (reAd5) (p < 0.05). Moreover the use of Ad5/3 reduced transgene expression by more than 90% in normal human brain cells compared with reAd5. Finally, the use of CRAd5/3 inhibited tumor cell proliferation by 43% more than replication-competent wild-type virus in vitro (p < 0.05). Conclusions The results of this study demonstrate that the Ad5/3 vector offers superior transduction efficiency and low toxicity in the setting of brain tumors, and therefore represents a potential new approach to gene therapy for malignant gliomas.

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