scholarly journals Studies on Chemical Composition and Antioxidant Activity of Rudbeckia triloba

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Zenovia Moldovan ◽  
Mihaela Buleandră ◽  
Eliza Oprea ◽  
Zamfirica Mînea
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Molecular Weights ◽  
Aerial Parts ◽  
Phenolic And Flavonoid ◽  
Vegetable Material

The paper describes the physicochemical studies made on the decorative plant, Rudbeckia triloba (Asteraceae). For this purpose, essential oil, infusion, decoction, and hydroalcoholic macerate obtained from different aerial parts of Rudbeckia triloba were analyzed. The main phytochemical constituents identified by GC-MS analysis were found to be α-pinene (in dried leaves (46.0%) and flowers (40.1%)) and β-phellandrene (in essential oil of dried inflorescences (26.09%)). The Folin–Ciocalteu and quercetin assays revealed different values of total phenolic and flavonoid contents of petals, leaves, and seeds as a function of the solvent used and extraction procedure. The hydroalcoholic macerate of petals was found to present the maximum phenolic and flavonoid contents (130.29 ± 5.58 mg gallic acid equivalent/g dry vegetable material and 30.72 ± 1.35 mg quercetin equivalent/g dry vegetable material, resp.) and also exhibits the lower value of EC50 (0.32% (v/v)), obtained by applying the DPPH⋅ assay. Comparing the extraction methods applied, the maceration was found to be the most effective for phenolic compounds, most likely due to the solvent (70% ethanol). The use of water-alcohol mixture leads to an improvement of the extraction yield of phenolic compounds (including those with higher molecular weights) than by using water as extractive solvent, in the case of infusions and decocts.

scholarly journals Phenolic Compounds from An Algerian Endemic Species of Hypochaeris laevigata var. hipponensis and Investigation of Antioxidant Activities

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 514 ◽  
Author(s):  
Nabila Souilah ◽  
Zain Ullah ◽  
Hamdi Bendif ◽  
Kamel Medjroubi ◽  
Tahar Hazmoune ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Quinic Acid ◽  
Total Phenolic ◽  
Antioxidant Action ◽  
Endemic Plant ◽  
Aerial Parts ◽  
Phenolic Constituents ◽  
First Time ◽  
Phenolic And Flavonoid

Hypochaeris laevigata var. hipponensis (Asteraceae) is an endemic plant from Algeria. In the current study, we analyzed for the first time its chemical composition, especially phenolic constituents of dichloromethane (DCM), ethyl acetate (EA), and n-butanol (BuOH) fractionsof the aerial parts of Hypochaeris laevigata var. hipponensis by liquid chromatography-mass spectrometry (LC-MS/MS). The number of phenolic compounds detected in DCM, EA, and BuOH fractions were found to be 9, 20, and 15, respectively. More specifically, 12 phenolic acids were detected. Among them, quinic acid, chlorogenic acid, and caffeic acid were the most abundant ones. Meanwhile, only seven flavonoids were detected. Among them, rutin, apigetrin, and isoquercitrin were the major ones. We also determined the total phenolic and flavonoid contents, and fraction EA showed the highest values, followed by BuOH, and DCM fractions. Furthermore, the antioxidant action was dictated by five methods and the tested plant fractions demonstrated a noteworthy antioxidant action.

scholarly journals Volatile Fraction Composition and Total Phenolic and Flavonoid Contents of Elionurus hensii—Antioxidant Activities of Essential Oils and Solvent Extracts

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yin Yang ◽  
Marie-Cécile De Cian ◽  
Samuel Nsikabaka ◽  
Pierre Tomi ◽  
Thomas Silou ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Volatile Fraction ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Phenolic And Flavonoid

The objective of this study was to characterize Elionurus hensii in terms of the chemical composition of its essential oil and volatile fractions, the total phenolic and flavonoid contents of solvent extracts and antioxidative activity. Analysis of E. hensii essential oil samples from Congo was carried out using Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (MS). The essential oil of the aerial parts consisted predominantly of oxygenated monoterpene compounds, of which the main components were menthane alcohol stereoisomers, whereas the essential oil of the root was characterized by a high content of oxygenated sesquiterpenes, the main component of which was aristolone. The amounts of total phenolics and flavonoids in the solvent extracts (dichloromethane, ethyl acetate and methanol) were determined spectrometrically. The essential oil did not exhibit significant antioxidant activity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS•+) scavenging ability, and exhibited only slight Fe3+ reducing ability. In contrast, the antioxidant activity of the solvent extracts of the aerial parts increased in the order ethyl acetate > methanol > dichloromethane, and that of the solvent extracts of the root increased in the order methanol > ethyl acetate > dichloromethane. Finally, a relationship was observed between the antioxidant activity and the total phenolic and flavonoid levels of the extract.

Microwave Pretreatment of Vegetable Materials to Increase the Extraction Yield of Natural Products

2018 ◽  
Vol 69 (8) ◽  
pp. 1976-1979
Author(s):  
Ioana Asofiei ◽  
Ioan Calinescu ◽  
Adina Ionuta Gavrila ◽  
Daniel Ighigeanu ◽  
Diana Martin
Keyword(s):  
Natural Products ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Microwave Pretreatment ◽  
Pretreatment Time ◽  
Conventional Extraction

It was designed and built a laboratory experimental installation (LEI) for the microwave pretreatment of vegetable materials. To study the influence of microwave pretreatment on the total phenolic content (TPC), a conventional extraction of polyphenols from treated and untreated fresh sea buckthorn leaves was performed. For short extraction times, the amount of phenolic compounds was higher for the extracts obtained from treated leaves, but a long pretreatment time (28 s) led to a decrease in TPC. The qualitative analysis showed that the chemical composition is not affected by the microwave pretreatment.

scholarly journals Obtaining Bioactive Compounds from the Coffee Husk (Coffea arabica L.) Using Different Extraction Methods

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 46
Author(s):  
Mariana de Oliveira Silva ◽  
John Nonvignon Bossis Honfoga ◽  
Lorena Lucena de Medeiros ◽  
Marta Suely Madruga ◽  
Taliana Kênia Alencar Bezerra
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Extraction Methods ◽  
Water Bath ◽  
Raw Material ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ethanol Mixture ◽  
Coffee Husk ◽  
Coffea Arabica L

Coffee husks (Coffea arabica L.) are characterized by exhibiting secondary metabolites such as phenolic compounds, which can be used as raw material for obtaining bioactive compounds of interest in food. The objective of this study is to evaluate different methods for obtaining the raw material and extracting solutions of bioactive compounds from coffee husks. Water bath and ultrasound-assisted extraction methods were used, using water (100%) or ethanol (100%) or a mixture of both (1:1) as extracting solutions and the form of the raw material was in natura and dehydrated. The extracts were evaluated by their antioxidant potential using DPPH radicals, ABTS, and iron reduction (ferric reducing antioxidant power (FRAP)), and later total phenolic compounds, total flavonoids, and condensed tannins were quantified the phenolic majority compounds were identified. It was verified that the mixture of water and ethanol (1:1) showed better extraction capacity of the compounds with antioxidant activity and that both conventional (water bath) or unconventional (ultrasound) methods showed satisfactory results. Finally, a satisfactory amount of bioactive compounds was observed in evaluating the chemical composition (total phenolic compounds, total flavonoids, condensed tannins, as well as the analysis of the phenolic profile) of these extracts. Corroborating with the results of the antioxidant activities, the best extracting solution was generally the water and ethanol mixture (1:1) using a dehydrated husk and water bath as the best method, presenting higher levels of the bioactive compounds in question, with an emphasis on chlorogenic acid. Thus, it can be concluded that the use of coffee husk as raw material to obtain extracts of bioactive compounds is promising. Last, the conventional method (water bath) and the water and ethanol mixture (1:1) stood out among the methods and extracting solutions used for the dehydrated coffee husk.

scholarly journals Polyphenolic compounds and essential oil analysis of selected species of the genus Thymus / Analýza fenolových zložiek a analýza silice vybraných druhov rodu Thymus

2015 ◽  
Vol 62 (s9) ◽  
pp. 12-17
Author(s):  
M. Kameníková ◽  
S. Fialová ◽  
A. Ťažký ◽  
I. Čičová
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Spectrophotometric Method ◽  
Total Phenolics ◽  
Chemical Constituents ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Oil Analysis ◽  
European Pharmacopoeia

Abstract The content of phenolic compounds (total phenolic compounds, tannins, flavonoids and anthocyanidins) of three species of thyme (Thymus pulegioides, Thymus pannonicus, Thymus praecox) of different origin (Bohemian-Moravian highlands, Křivoklat and Považsky Inovec) was determined using spectrophotometric methods of European Pharmacopoeia 8th edition. Furthermore, the determination of the content of essential oil and analysis of its constituents was realised. The amount of total phenolics was determined by a spectrophotometric method using the Folin-Ciocalteu reagent. Their content ranged from 3.87 to 8.86%. The content of tannins was established on a preliminary determination of the total phenolics, followed by adsorption of tannins on hide powder. The amount of tannins in our samples varied from 1.96 to 5.65%. The content of total flavonoids was determined by a spectrophotometric method using aluminium chloride. Quantitative analysis of flavonoids has shown that content in our samples ranged from 0.59 to 1.52% expressed as luteolin-7-O-glucoside (λ = 392 nm) and from 0.41 to 1.12% expressed as rutin (λ = 420 nm). Anthocyanins represent a small amount of total polyphenol content in Thymus species. In our samples, the content of anthocyanins expressed as cyanidin-3-O-glucoside varied from 0.02 to 0.1%. The determination of essential oil was realised by method of European Pharmacopoeia 8th edition. Its contents in our samples ranged from 0.2 to 0.75%. Gas chromatography-mass spectrometry was used for essential oil analysis. According to the presence of main monoterpene in Thymus essential oil, we can distinguish three chemotypes: thymol, carvacrol and linalool. Due to variations of chemical constituents of thyme essential oil, it was possible to observe a relationship between occurrence of certain chemotype and origin of sample. Differences in content of flavonoids have not shown a significant relationship to locality of origin.

Investigation of antioxidant activity and analysis of phenolic compounds of some Asteraceae plants by HPLC: A comparison between Methanol and Ethanol extracts

2021 ◽  
Vol 02 ◽  
Author(s):  
Donia Anvari ◽  
Rashid Jamei
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Sinapic Acid ◽  
Total Phenolic ◽  
Therapeutic Effects ◽  
Antioxidant Power ◽  
Antioxidant Agents ◽  
Ethanol Extracts

Background: Medicinal and healing plants have been used in treating human diseases for centuries because of their therapeutic effects. They may assist in curing common ailments such as a multitude of skin problems, various disorders from muscle spasms to cuts and wounds. They can be used to relieve symptoms of different illnesses from a cold to some forms of arthritis or some allergies as well. The Asteraceae plant is a strong source of antimicrobial and antioxidant agents and this paper focuses on its specifications. Objectives: This study aimed to investigate the antioxidant potential and radical scavenging of different solvents (Methanol and Ethanol) of five species, i.e., Artemisia absinthium L., Arctium lappa L., Centaurea cyanus L., Silybum marianum L., and Echinops ritro L., belonging to the Asteraceae family. Methods: Methanol and ethanol extracts of the above plants were prepared. The obtained sections were evaluated for total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), chain-breaking activity (CBA), thiobarbituric acid (TBA), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Also, Ferric reducing antioxidant power (FRAP), Nitric oxide (NO) radical scavenging, Hydrogen peroxide (H2O2) radical scavenging, and Superoxide (O2-) radical inhibition assay were measured. Phenolic compounds were determined and measured by high-performance liquid chromatography (HPLC) as well. Results: The collected and analyzed data showed that the highest values for the TPC, TFC, TBA, and DPPH were related to methanol extract of A. lappa L. Moreover, the maximum values for the CBA, H2O2, and O2- were observed in the Ethanol extract of E. ritro L., while methanol extract of E. ritro L. showed the highest amount of FRAP and NO. Eventually, the highest value for TAC was related to A. absintium L., and it was also realized that methanol compared to ethanol solvent was more successful in the extraction procedure. Conclusions: These findings suggest that A. lappa L. and E. ritro L. extracts can be considered excellent natural antioxidant agents. The type of solvent can affect the extraction of phenolic compounds. Sinapic acid, as the highest level of phenolic acid, was found in S. marianum L.

scholarly journals NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

2017 ◽  
Vol 9 (2) ◽  
pp. 166 ◽  
Author(s):  
Sayed A. El-toumy ◽  
Joslin Y. Salib ◽  
Nabila H. Shafik ◽  
Asmaa S. Abd Elkarim ◽  
Gihan A. Mick
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Secondary Metabolites ◽  
Gallic Acid ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Dpph Method ◽  
Phenolic And Flavonoid

<p><strong>Objective: </strong>The current study was to deal the isolation and identification of secondary metabolites from <em>Polygonum equisetiforme</em> and evaluation of antioxidant activity of its extract.</p><p><strong>Methods: </strong>The methanol-water extract (7:3) of the air-dried aerial parts of <em>Polygonum equisetiforme</em> was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for <em>in vitro</em> antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.</p><p><strong>Results: </strong>Ten secondary metabolites were isolated from <em>Polygonum equisetiforme</em> in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-<em>O-β</em>-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-<em>O-β</em>-D-glucucorinde methyl ester (4), quercetin 3-<em>O-</em>β-D-glucopyranoside (5), quercetin 7-<em>O-β-</em>D-glucopyranoside (6),<em> </em>quercetin(7)<sub>, </sub>myricetin (8), <em>P</em><sub>-</sub>methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of <em>P. equisetiforme</em> extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC<sub>50 </sub>= 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.</p><p><strong>Conclusion: </strong><em>Polygonum equisetiforme</em> is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.</p>

scholarly journals Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss. from Macedonia

2014 ◽  
Vol 66 (1) ◽  
pp. 401-413 ◽  
Author(s):  
Ksenija Mileski ◽  
Ana Dzamic ◽  
Ana Ciric ◽  
Slavica Grujic ◽  
M. Ristic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts ◽  
Positive Controls

This study was undertaken to determine the antioxidant and antimicrobial effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam. Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana essential oil was characterized by the presence of methyl eugenol (60.40%), p-cymene (11.18%) and ?-phellandrene (10.23%). The free radical scavenging activity of extracts and essential oil was evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger antioxidant activity compared to hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and ABTS tests, respectively. The total phenolic and flavonoid concentrations in the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The antimicrobial properties of the extracts and essential oil were investigated using a micro-well dilution technique against human pathogenic strains. The results were comparable with the effects of the positive controls, streptomycin and fluconazole. These findings indicate that E. sibthorpiana extracts and oil can be used in preventive treatments and as an alternative for synthetic preservatives.

scholarly journals Total phenolic and flavonoid contents and biological activities of Cachrys cristata DC. extracts

2014 ◽  
Vol 66 (3) ◽  
pp. 1117-1123
Author(s):  
Jelena Matejic ◽  
Ana Dzamic ◽  
Tatjana Mihajilov-Krstev ◽  
Vladimir Randjelovic ◽  
Ksenija Mileski ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Antimicrobial Agents ◽  
Biological Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Potential Source ◽  
Dilution Assay ◽  
Phenolic And Flavonoid

The total phenolic/flavonoid contents and antioxidant potential of the methanol, ethyl-acetate, acetone and water extracts obtained from the aerial parts and fruits of Cachrys cristata DC.(Apiaceae) were compared. The total phenolic contents of the tested extracts were determined using Folin-Ciocalteu?s reagent. The amounts per g of dry plant extract of gallic acid (GA) and quercetin (Qu) ranged between 22.60-166.97 mg, and 8.91-46.02 mg, respectively. The antioxidant activity, expressed as IC50, ranged from 1.784-17.621 mg/mL and from 1.01-3.42 mg L(+)-ascorbic acid (Vitamin C)/g when tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ABTS, respectively. The antimicrobial activity of the extracts was investigated by the microwell dilution assay, for the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and yeast Candida albicans ATCC 10231. The results indicate that C. cristata can be regarded as a potential source of antioxidant and antimicrobial agents.

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