scholarly journals Protective Effect of a Polyherbal Aqueous Extract Comprised ofNigella sativa(Seeds),Hemidesmus indicus(Roots), andSmilax glabra(Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Bandula Prasanna Galhena ◽  
S. S. R. Samarakoon ◽  
Myrtle Ira Thabrew ◽  
Solomon F. D. Paul ◽  
Venkatachalam Perumal ◽  
...  
Keyword(s):  
Protective Effect ◽  
Chromosomal Aberrations ◽  
Chromosome Aberrations ◽  
Nigella Sativa ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Cytogenetic Damage ◽  
Hemidesmus Indicus ◽  
Human Pbls ◽  
Different Doses

This study was carried out to determine the chemoprotective potential of a polyherbal aqueous decoction comprised ofNigella sativa(seeds),Hemidesmus indicus(roots), andSmilax glabra(rhizome) against bleomycin induced cytogenetic damage in human lymphocytes. Isolated peripheral blood lymphocytes (PBLs) were exposed to bleomycin at a dose of 40 µg/mL for 2 hrs in the presence or absence of different doses of the decoction (100, 300, and 600µg/mL). Modulatory effect of the decoction on bleomycin induced cytogenetic damage was evaluated by (a) degree of chromosomal aberrations (CA), (b) formation of micronuclei (MN), and (c) induction ofγH2AX foci in lymphocytes exposed to bleomycin. Lymphocytes pretreated with the decoction showed that a significant reduction (p<0.05) in bleomycin induced (a) stable and unstable chromosome aberrations (CA), (b) MN formation, and (c) formation ofγH2AX foci, when compared to lymphocytes treated only with bleomycin. The decoction by itself did not induce any significant cytogenetic damage in PBLs. Overall results of the present study confirm that the decoction can attenuate the cytogenetic damage mediated by bleomycin in human PBLs.

Protective effect of Nigella sativa seeds against spermatocyte chromosomal aberrations and genotoxicity induced by carbon tetrachloride in mice

2017 ◽  
Vol 24 (12) ◽  
pp. 11677-11682 ◽  
Author(s):  
Ashraf M. Abdel-Moneim ◽  
Amina E. Essawy ◽  
Sherifa S. Hamed ◽  
Ashgan A. Abou-Gabal ◽  
Aglal A. Alzergy
Keyword(s):  
Carbon Tetrachloride ◽  
Protective Effect ◽  
Chromosomal Aberrations ◽  
Nigella Sativa

scholarly journals Micronucleus Assay as a Biological Indicator for In Vitro Exposure of Human Lymphocytes to Gamma Rays

2012 ◽  
Vol 6 (1) ◽  
pp. 51-55
Author(s):  
Abbas N. Balasem
Keyword(s):  
Gamma Rays ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Biological Indicator ◽  
Peripheral Blood Lymphocytes ◽  
Radiation Accidents ◽  
In Vitro Exposure ◽  
Different Doses

Micronucleus Assay was employed to detect the effects of acute exposure of human peripheral blood lymphocytes in vitro to Cs -137 gamma rays. Human whole blood samples were irradiated with different doses of gamma rays namely ) 0.1, 0.2, 0.3, 0.4, 0.5, 1.00) Gy, respectively in addition to a control non-irradiated sample. The samples were tissue cultured and cytokinesis blocked method was used to investigate the frequency of micronuclei. In vitro exposure of lymphocytes to this doses led to elevation of micronuclei in comparison with non –irradiated samples However, inclusion of mono-, tri-,and quadrinucleated cells in micronucleus assay probably gives more satisfying result than restriction the test on binucleated cells. Computed programmed were employed to establish dose – response relationships to be used as biological dosimeter during radiation accidents.

scholarly journals Investigation of the Immune Modulatory Potential of Zinc Oxide Nanoparticles in Human Lymphocytes

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 629
Author(s):  
Helena Moratin ◽  
Pascal Ickrath ◽  
Agmal Scherzad ◽  
Till Jasper Meyer ◽  
Sebastian Naczenski ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Immune Modulation ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Drug Carriers ◽  
Physiological Model ◽  
Peripheral Blood Lymphocytes ◽  
Major Influence ◽  
Oxide Nanoparticles

Zinc oxide nanoparticles (ZnO-NP) are commonly used for a variety of applications in everyday life. In addition, due to its versatility, nanotechnology supports promising approaches in the medical sector. NP can act as drug-carriers in the context of targeted chemo- or immunotherapy, and might also exhibit autonomous immune-modulatory characteristics. Knowledge of potential immunosuppressive or stimulating effects of NP is indispensable for the safety of consumers as well as patients. In this study, primary human peripheral blood lymphocytes of 9 donors were treated with different sub-cytotoxic concentrations of ZnO-NP for the duration of 1, 2, or 3 days. Flow cytometry was performed to investigate changes in the activation profile and the proportion of T cell subpopulations. ZnO-NP applied in this study did not induce any significant alterations in the examined markers, indicating their lack of impairment in terms of immune modulation. However, physicochemical characteristics exert a major influence on NP-associated bioactivity. To allow a precise simulation of the complex molecular processes of immune modulation, a physiological model including the different components of an immune response is needed.

The Dose Response of Chromosome Aberrations in Human Lymphocytes InducedIn Vitroby Very Low-Dose γ Rays

2011 ◽  
Vol 175 (2) ◽  
pp. 208-213 ◽  
Author(s):  
Toshiyasu Iwasaki ◽  
Yoshio Takashima ◽  
Toshikazu Suzuki ◽  
Mitsuaki A. Yoshida ◽  
Isamu Hayata
Keyword(s):  
Dose Response ◽  
Chromosome Aberrations ◽  
Low Dose ◽  
Human Lymphocytes ◽  
Γ Rays

The Protective Effect of α-Hederin, the Active Constituent of Nigella sativa , on Lung Inflammation and Blood Cytokines in Ovalbumin Sensitized Guinea Pigs

2015 ◽  
Vol 29 (11) ◽  
pp. 1761-1767 ◽  
Author(s):  
Rana Keyhanmanesh ◽  
Saeideh Saadat ◽  
Mostafa Mohammadi ◽  
Amir-Ali Shahbazfar ◽  
Maryam Fallahi
Keyword(s):  
Protective Effect ◽  
Guinea Pigs ◽  
Lung Inflammation ◽  
Nigella Sativa ◽  
Active Constituent ◽  
Blood Cytokines

Chromosome aberrations in peripheral blood lymphocytes of high-risk HPV-infected women with HGSIL

2008 ◽  
Vol 49 (9) ◽  
pp. 688-694 ◽  
Author(s):  
Rosa E. Álvarez-Rosero ◽  
Jannet Rodríguez-Argote ◽  
Yexania Y. Arboleda-Moreno ◽  
Sulma L. Muñoz-Benítez ◽  
Carlos H. Sierra-Torres
Keyword(s):  
High Risk ◽  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
High Risk Hpv
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